regulation of iron metabolism in mammalian cells
báo cáo khoa học: "Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy" pps
Ngày tải lên :
11/08/2014, 00:23
... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... EMT cells after co-culturing with 500 μM naked AuNPs for 48 h The size distribution in A) was obtained by analyzing individual cells such as that in B) Bars in B: 10 μm Chen et al Journal of ... Figure 8C and 8D (movie of different projection in Additional file 2) shows the results of specimen staining in revealing the detailed localization and shape of the nucleus and of the overall nanoparticle...
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Regulation of antigen presentation in dendritic cells
Ngày tải lên :
11/09/2015, 16:05
... proposed that cystatin C might predominantly control the regulation of CD74 protein degradation via the inhibition of activity of cathepsin S which was essentially involved in generating CLIP (Pierre ... donor Since the trafficking of MHC class II is CD74dependent, the increase in cell surface localisation of MHC class II in maturing DCs could be partly due to the increase in CD74 protein expression ... DCs 48 Figure Inhibition of phagocytosis during maturation of DCs 50 Figure Increase in NO production during maturation of DCs 51 Figure 10 RT-PCR results showing a new isoform of DC-CIITA 52...
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Báo cáo Y học: Regulation of peptide-chain elongation in mammalian cells pptx
Ngày tải lên :
23/03/2014, 21:20
... calmodulin binding and the GxGxxG motif that may be involved in substrate binding (see text) within this motif prevents calmodulin binding and thus also activity [43] Removal of the C-terminus (residues ... sources Instead, activation of PKA results in a marked rise in the maximal activity of eEF2 kinase from ventricular myocytes, suggesting that these cells may contain a distinct isoform of eEF2 kinase, ... existence of multiple forms of eEF2 kinase in phaeochromocytoma cells and the regulatory properties of eEF2 kinase in ventricular cardiomyocytes suggest the existence of a distinct form in these cells...
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Genetics of golgi apparatus regulation in mammalian cells
Ngày tải lên :
10/09/2015, 09:06
... secretory cells It was found that while insulin producing β cells of mice are 3.7-5.8µm3, the Golgi stack comprised of 3.1-3.6µm3 [123] Finally, unlinking of the ribbon is an intrinstic G2/M checkpoint ... activation of SFKs at the Golgi was observed upon increase in incoming secretory cargo [145] This appears to be mediated by the binding of KDEL receptor with incoming luminal chaperones entering the ... glycoproteins that are heavily O-glycosylated, line the surfaces of epithelial cells and goblet or mucous cells of the tracheobronchial, gastrointestinal, and genitourinary tracts In the gastrointestinal...
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Molecular mechanisms underlying the regulation of the positioning and formation of the cleavage furrow in cytokinesis in mammalian cells
Ngày tải lên :
14/09/2015, 14:09
... populations of microtubules in mitotic spindle in positioning of the cleavage furrow 1.2.1.1 Roles of microtubules in positioning of the cleavage furrow Pioneering studies in marine invertebrates ... action Inhibit myosin II ATPase activity by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release of the ADP Inhibit actin polymerization by binding to the barb end of the FCytochalasin ... cytokinesis in mammalian cells requires remodeling of cortical actin network mediated by αactinin in coordination with myosin based contraction 1.2.4 Abscission Abscission is the last step of animal...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
Ngày tải lên :
19/02/2014, 18:20
... maintained in the cells by binding to antigen Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular colocalization was the result of ... 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP proteins allows them to be maintained in the cells To rule out the possibility that the EGFP part of ... measuring protein mobility and activity in living cells Nature Cell Biol 3, 145–147 Bachmair A, Finley D & Varshavsky AK (1986) In vivo half-life of a protein is a function of its amino-terminal...
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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt
Ngày tải lên :
14/03/2014, 23:20
... be induced by incubating cancer cells with oligomycin, an inhibitor of mitochondrial ATP synthetase [159,160] Similarly, reduction of OXPHOS by targeted disruption of frataxin, a protein involved ... enzyme inactive One of the four known mammalian isoforms of the pyruvate dehydrogenase kinase (PDHK-1) (EC 2.7.11.2) has been shown to be inducible by HIF-1 in renal carcinoma cells and in a human ... only expressed in intestinal and renal epithelial cells and endothelial cells at the blood–brain barrier Metabolic control analysis of glycolysis in AS-30D carcinoma and HeLa cells provided evidence...
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Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx
Ngày tải lên :
16/03/2014, 12:20
... DNA binding domain and VP16 activation domain Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig 1A) and tested in NIH ... probably in uence the behavior of fusion protein upon binding to ligand Apparently, having activation domain (VP16) and DNA binding domain (GAL4), in that order, on the NH2-terminal end of EcR ... formats Interestingly, this is the arrangement in natural EcRs [activation domain A ⁄ B, DNA binding domain (C) and ligand binding (E) domain] The difference in induction levels between cell lines...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
Ngày tải lên :
16/03/2014, 13:20
... eukaryotic cells including human cells [7] Targets of the kinase in the regulation of DNA replication include ORC2, Cdc6 and Mcm proteins in Saccharomyces cerevisiae [8], and disregulation of these ... prevent binding of MCM complex to chromatin [12] In contrast, a recent finding suggests that an intermediate level of phosphorylation of MCM4 is required for chromatin binding during interphase ... cell cycle in a site-specific manner Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4 To determine which kinase is involved in the phosphorylation of MCM4 in phases G2...
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Báo cáo khoa học: Calcium-induced contraction of sarcomeres changes the regulation of mitochondrial respiration in permeabilized cardiac cells doc
Ngày tải lên :
16/03/2014, 22:20
... reported in this work, the decrease in the Vmax of respiration with an increase in the free calcium concentration (Fig 6A) is of interest, and may be explained by an inhibitory effect of increased ... Reversibility of the effects of calcium-induced contraction of cardiomyocytes on the kinetics of regulation of mitochondrial respiration by exogenous ADP The kinetics of respiratory regulation was ... creatine kinase reactions, including mitochondrial creatine kinase [3840] Increase of the free calcium concentration to lm resulted in some decrease of the Vmax, but its value stayed higher in...
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Báo cáo khoa học: Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx
Ngày tải lên :
17/03/2014, 10:20
... examined the effects of SA on stress response in mammalian cells using a simple screening system, and revealed that SA is a potent Hsp inducer in mammalian cells, thereby protecting cells against ... transcription of hsp genes and the accumulation of Hsps in various mammalian cells and a concomitant increase of thermoresistance of cells Thus, SA may be used for the protection of cells against deleterious ... containing a geneticin resistant gene (3 lg) were cotransfected into C3H10T1/2 cells (1 · 107 cells per 100 mm dish) with 30 lL lipofectAMINE reagent (Invitrogen) according to the manufacturer’s instructions,...
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Báo cáo Y học: Mydj2 as a potent partner of hsc70 in mammalian cells doc
Ngày tải lên :
17/03/2014, 17:20
... R.A., Narain, Y., Rankin, J & Rubinsztein, D.C (2000) Effects of heat shock, heat shock protein 40 (HDJ-2) and proteosome inhibition on protein aggregation in cellular models of Huntington’s disease ... to bind to immobilized Mydj2-His protein (lanes 3, 3¢) In contrast, no binding between the immobilized dj2 protein and the inducible hsp70 protein was observed (Fig 6, lanes: 3, 3¢) Interestingly, ... and incubated in 3% BSA in Ó FEBS 2002 Monkey dj2, a specific partner for hsc70 (Eur J Biochem 269) 1555 NaCl/Pi to prevent nonspecific staining After h of incubation with blocking medium, the cells...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
Ngày tải lên :
17/03/2014, 17:20
... the increased rate of 13C label incorporation into the C6 position of glycogen in the ADM group is indicative of activated 6-phosphofructo-1-kinase, which can lead to increased 13C labeling at ... start of glucose infusion For the calculation of glycogen C6 changes, data acquired within h of the start of the glucose infusion was not included to avoid any in uence of transient changes in the ... up -regulation of glucokinase and a down -regulation of glucose-6-phosphatase gene expression, suggesting that this biofactor may also be involved in balancing the uptake and release of glucose from...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
Ngày tải lên :
30/03/2014, 03:20
... core+1 protein in mammalian cells have failed, including transfection of cells with plasmid DNA encoding the core sequence or infection with recombinant herpes simplex virus expressing the core–E1–E2 ... that its detection in mammalian cells and in the HCV infectious system is elusive This study shows that intracellular levels of the core+1 protein in mammalian cells are strongly in uenced not only ... S–myc-expressing plasmid (pHPI-1496) and increasing amounts of the core-expressing vector (pHPI-1499) (Fig 2A), in the Expression of the HCV-1 core+1 protein presence of MG-132 Immunoblotting indicated...
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Báo cáo khoa học: Transient increase of the labile iron pool in HepG2 cells by intravenous iron preparations Brigitte Sturm, Hans Goldenberg and Barbara Scheiber-Mojdehkar doc
Ngày tải lên :
31/03/2014, 07:20
... concentration of iron from the preparation and rate of increase of the labile iron pool, using HepG2 human hepatoma cells as a cell culture model The initiation of translation of ferritin by an increase in ... in impairment of synthesis of iron containing proteins or in cell injury by pro-oxidants [43], such findings in nonreticuloendothelial cells may have important implications in the generation of ... that the cells adapt their iron metabolism according to the size of incoming iron by highly increasing ferritin biosynthesis as a means of protection from further iron loading LIP levels return to...
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Báo cáo y học: "Identification of secondary targets of N-containing bisphosphonates in mammalian cells via parallel competition analysis of the barcoded yeast deletion collection" ppsx
Ngày tải lên :
09/08/2014, 20:20
... a haploinsufficient profile in yeast in the presence of N-BPs Among these was ALF1, a homolog of the mammalian tubulin cofactor B (TBCB) gene, which encodes the α-tubulin folding protein It has ... the initiation of the DNA replication by activating the minichromosome maintenance (MCM) protein Both DBF4-Cdc7 and MCM proteins are phosphorylated by the protein kinases ATM and ATR [25] Cells ... of DBF4 in the nuclei of cells treated with N-BPs is particularly evident in the merged picture (Figure 3b) Interestingly, DBF4 appeared to have a molecular weight of about 118 kDa, instead of...
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Báo cáo y học: "Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells" pptx
Ngày tải lên :
10/08/2014, 05:21
... the GST-miniprecursor fusions are competent for autoprocessing in mammalian cells Darunavir and Indinavir inhibit fusion precursor autoprocessing in transfected 293T cells To further examine autoprocessing ... antibodies In the presence of cell-permeable Darunavir and Indinavir [26,27], precursor autoprocessing was inhibited in a dose-dependent manner, as indicated by the appearance of increasing amounts of ... proximal site) In the present study, a similar fusion construct was engineered for mammalian expression to examine protease autoprocessing in transfected mammalian cells Autoprocessing of the fusion...
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báo cáo khoa học: " Regulation of hypoxia inducible factor-1a expression by the alteration of redox status in HepG2 cells" pdf
Ngày tải lên :
10/08/2014, 10:21
... L-g-glutamyl-L-cysteinyl-glycine, a ubiquitous endogenous antioxidant It plays an important role in maintaining intracellular redox equilibrium and in augmenting cellular defenses in oxidative stress [20,21] In ... glutathione is in the reduced form Shifting redox equilibrium is in favor of a reducing or oxidizing state; that is in modification of the redox status in cells [22,23] The g-glutamylcysteine sythetase ... observed in HepG2 cells It is then very clear that HIF-1a proteins in hypoxic cells were significantly decreased with BSO concentrations gradually increasing In addition, the inhibition of HIF-1a...
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báo cáo khoa học: "Transcriptome analysis by GeneTrail revealed regulation of functional categories in response to alterations of iron homeostasis in Arabidopsis thaliana" pdf
Ngày tải lên :
11/08/2014, 11:20
... successfully applied for data mining The main strength of GeneTrail was that it offered answers to individual biological questions with its feature of incorporation of individually defined categories (such ... overlap in regulatory patterns may become apparent, e.g through assembly of individual pyrimidine, purine and nucleoside metabolism into a large nucleoside/nucleotide metabolism category, or of individual ... strength of microarray interpretation lies in the possibility of extracting information beyond the single gene level to address questions on the co -regulation of genes, on the identification of gene...
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Báo cáo y học: " Differential regulation of neurotrophin expression in human bronchial smooth muscle cells" pptx
Ngày tải lên :
12/08/2014, 16:20
... neurotrophins are operating in human airway inflammation The source and regulation of neurotrophin expression in the airways is not fully understood A local NGF production by structural cells, including ... h of culture (not shown) Effects of IL-1β on neurotrophin mRNA and protein expression We next investigated the ability of the proinflammatory cytokine IL-1β to induce neurotrophin expression in ... involvement of COX in the induction of BDNF but not of NGF To test this, we evaluated the effects of the COXinhibitors indomethacin and NS-398 (both 10 µM) on the secretion of these two neurotrophins...
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