real time visualization of axonal transport in neurons

... acquiring high quality confocal images (discussed in [12] The resulting timecourse reflecting the conformational change of the probe due to the binding of cyclic AMP was analysed in terms of (1) time ... full agonists in their studies into the kinetics of recombinant b2-adrenoceptor activation in HEK293 and CHO cells following exposure to both endogenous (epinephrine and norepinephrine) and synthetic ... construct was a kind gift from Dr Kees Jalink (NKI, Amsterdam, Netherlands) Lipofectamine 2000 was from Invitrogen (Paisley, UK) Results Real time emission ratio imaging of Epac-based probe In a previous...

Ngày tải lên: 12/08/2014, 13:22

... pairs of points in the time series where the  threshold P32 =P40 ¼ was crossed in the increasing direction The cycle time is then the time between crossings of this section Noise sometimes caused ... appearance of a cluster of repeated crossings of the section In these cases, we averaged the crossing times in a cluster Calculation of the cycle time based on the absolute maximum of each cycle ... with a computation of the mutual information I(k) between points in the time series separated by k time intervals, i.e between points i and i + k for all values of i The mutual information I(k)...

Ngày tải lên: 07/03/2014, 10:20

... first in the list 35% of the time, while it was in the first three 59% of the time We also simulated the effect of collapsing words in insertion-substitution sequences to allow corrections of insertions ... expected since the matrix has been trained explicitely for that purpose The performance should increase in the future as the amount of training data grows In comparison, the contribution of words ... presented in a list box under each word These lists are always present, instead of being presented only upon selection of a word In this way the user has the opportunity of scanning the lists in advance...

Ngày tải lên: 08/03/2014, 03:20

... is indispensable for information intake and processing within the central nervous system Glutamate neurotransmission is crucial in information intake and information processing within the brain ... blood brain barrier (BBB) is seen early in neuroinflammation, and parallels the release of proinflammatory cytokines [26-28] Mechanisms for disruption of the BBB in neuroinflammation are incompletely ... starts early in some neurodegenerative disease such as Alzheimer's and Parkinson's diseases, being prominent for long time in these diseases and also in neuroinflammatory diseases, in meningitis,...

Ngày tải lên: 19/06/2014, 22:20

... activation in MS lesions Extensive IL-16 immunostaining, confined to infiltrating cells, was observed in MS lesions Less widespread immu- nostaining was found in normal-appearing white matter of brain ... severity of relapsing disease, of inflammation, and of demyelination, as well as the extent of axonal damage [19] We provided evidence of regulation of IL-16 in EAE, which suggested that production of ... markedly increased levels of phosphorylated medium and heavy chains of neurofilament [NF (M+H)P] in MS lesions Relative levels of NF (M+H)P are markedly increased in MS lesions in (A) brain and spinal...

Ngày tải lên: 19/06/2014, 22:20

... synthesis The functions of Enh1 and EnhII were demonstrated for the HBV ayw subtype [4] Protein binding sites DNA binding sites within the central core domain of Enh I Binding sites of C/EBP, p53, IRFα, ... annotated on most HBV genomes It is involved in regulation of translation of the P and C CDSs and is conserved in all HBV genotypes [31] Protein domains: P – Terminal protein, Spacer, Reverse transcriptase, ... overview of the entire application information flow is shown schematically in Figure Figure Schematic overview of the information flow in HBVRegDB Schematic overview of the information flow in HBVRegDB...

Ngày tải lên: 20/06/2014, 01:20

... resulting in peaks in the ODF Instead of making predictions using only the bin magnitudes, the complex domain ODF [18] attempts to improve the prediction for the next value of a given bin using ... signals in the time domain can be mapped onto sinusoidal signals in a frequency domain, in this case, using the discrete cosine transform (DCT) [24] Roughly speaking, the DCT of a transient time- domain ... the limitations of strict real- time operation (defined in Section 2.1) As this article focuses on the evaluation of different ODFs in real- time, the peak-picking and thresholding processes are...

Ngày tải lên: 20/06/2014, 22:20

... resulting in peaks in the ODF Instead of making predictions using only the bin magnitudes, the complex domain ODF [18] attempts to improve the prediction for the next value of a given bin using ... signals in the time domain can be mapped onto sinusoidal signals in a frequency domain, in this case, using the discrete cosine transform (DCT) [24] Roughly speaking, the DCT of a transient time- domain ... the limitations of strict real- time operation (defined in Section 2.1) As this article focuses on the evaluation of different ODFs in real- time, the peak-picking and thresholding processes are...

Ngày tải lên: 21/06/2014, 00:20

... assurance of traffic safety - Norms of evaluating the friendly effect to environment - Norms of evaluating the uses of energy in public transport - Norms of evaluating the improvement of investment ... density in big cities, especially in Hanoi and Ho Chi Minh City, is more and more increasing, leading to the situation of increasing traffic congestion, decreasing average traffic speed, increasing ... failure of urban transport in the world in aspects of awareness, planning, development of public transport and intervention roles of Government (2) Evaluation on current situation of public transport...

Ngày tải lên: 02/07/2014, 20:37

... minimum is attained in a single point In the case of degeneracy, as commonly present in the IMRT setting, the set of minimizers is a sizeable, high dimensional, closed and connected subset of ... set of all beamlets of an arrangement of intensity modulated beams, or the set of all arclets of radiosurgery arcs The basis set defines the parameter space of the optimisation as the space of ... In ∗ ∗ a convex setting, it is possible to go from D1 to D2 along a straight line without leaving the solution space, but the gradient of F in the direction of ηΔ has to increase when ∗ ∗ going...

Ngày tải lên: 09/08/2014, 10:21

... extrema To delineate the extremes of local variation in ext AUC values, we divided each population’s values by determining local peaks and valleys in the data To determine inflection points in the values, ... than Extentmin Intersection of peaks with canonical coding regions To determine genes underlying each peak or peak region, we intersected their coordinates with a set of genes and coding regions ... study [30] showed, using simulation, the profound effect of increasing selection coefficients on the ability of the LDhat [60] recombination inference program to detect recombination This could...

Ngày tải lên: 09/08/2014, 22:23

... were arranged in the surface of the inner cavity of cylindiric Endothelial cells arranged plane originally is a rectangle which forming inner surface of the cylindrical inner cavity of ANs The borders ... destruction of the reflex arches of the ANs due to neurodegeneration in the DRG of L1–6 Although PNs injury has been reported as a cause of power loss at the involved muscles, injury to the feeding vessels ... loss at the involved muscles, but feeding vessels of PNs has not been reported in plegic subjects It has been reported that brain or spinal cord injuries cause neuronal degeneration in spine ganglia...

Ngày tải lên: 10/08/2014, 10:20

... originating from the leads The technology of an offline 3D TEE reconstruction technique helped us to identify that the mass was trapped between the leads of the PM and defined the origin of the ... images of the offline reconstruction AM and HT have been involved in drafting the manuscript and revising it critically for important intellectual content All authors read and approved the final ... OA, Greinacher A: Successful thrombolysis of right atrial and ventricle thrombi encircling a temporary pacemaker lead in a patient with heparin-induced thrombocytopenia type II Pacing Clin Electrophysiol...

Ngày tải lên: 11/08/2014, 02:22

... Denmark) Creatin kinase and the creatin kinase-myocardial bands were determined every hours (BM/Hitachi 717 analyser; Boehringer Mannhein, Inc., Mannheim, Germany) The creatin kinase/creatin kinase-myocardial ... norephinephrine infusion >0.5 µg/kg/min (Table 2) The number of positive inotropic agents necessary and the markers for myocardial ischemia (monitored by ECG), for creatin kinase/creatin kinase-myocardial ... values of tumor necrosis factor/cachectin, interleukin-1, interferonalpha, and interferon-gamma in the serum of patients with septic shock Swiss-Dutch J5 Immunoglobulin Study Group J Infect Dis...

Ngày tải lên: 12/08/2014, 22:21

... each of them was fitted with a diffusion/binding model [14] The assumptions made were that the binding sites are uniform within the volume of the spot and that their number does not change in time ... substantially in mass, pointing to a complex containing both proteins We found that Tat remained bound to nascent HIV-1 RNAs for 55 seconds, and diffused for 60 seconds between two binding events ... transcription machinery, including the Cterminal domain (CTD) of the large sub-unit of RNA polymerase II (RNAPII), and elongation factors DSIF and NELF [5] This transforms RNAPII into a highly processive...

Ngày tải lên: 13/08/2014, 05:22

... investigated time- range, meaning that the rate of increase, dL/dt, remains constant for at least some time in both the beginning and the ending time- periods) Depending on the actual data, this might in ... t (time; min) Time- profiles of the speed (rate) of length-growth (∆L/∆t for the experimental dataobtained from the best-fitting exponential Figurefunctions) (LinBiExp) types of (Exp) and bilinearfor ... length-growth increases [2-9] The difference between the two models is most evident in the time profiles of the speed (rate) of growth increases (dL/dt): that of the bilinear model contains two constant...

Ngày tải lên: 13/08/2014, 23:20

... domain is real- time instrumentation and monitoring of DRE systems In this paper, we compared the performance of using AJAX and WebSockets to support real- time instrumentation and monitoring of ... to internals of both technologies and how they are used in the domain of real- time monitoring while implementing web applications Real- time monitoring is inherently resource extensive domain ... using,) when using AJAX and WebSockets to monitor collected instrumentation data in real- time 5.1.1 Experiment Design & Setup Using the general experimental setup explained at the beginning of...

Ngày tải lên: 24/08/2014, 12:08

... Table 3.5: Contribution of real estate industry to whole economy of Vietnam in nine years at the beginning of 21 century 34 Table: 3.6: Index of housing price/personal income 36 Table ... point of view from investor when they are concerning about opportunity of increasing market value of company Therefore, FPT joined into real estate market in the end of year 2006 Likewise, in year ... Contribution of real estate industry to whole economy of Vietnam in nine years at the beginning of 21 century Contribution of real estate industry to whole economy LongYear Amount of Labor Capital...

Ngày tải lên: 24/11/2014, 01:34

... the local size of element none H The average depth of reservoir m A size of general ROM none Mu A size of ROM of velocity none Mc A size of ROM of contaminant none N The size of full order model ... variables in the system model The objective of control is to flush the contaminant out of the domain in a short time The problem may not be too difficult if we know exactly the location of the contaminant ... loading as a point in ow source of contaminant and developed an optimal control model to determine the optimal pollutant loads at different in ux points along the course of a river in order to reduce...

Ngày tải lên: 09/09/2015, 10:06

... with the on-line spectroscopic diagnostic framework has the potential to become a promising clinical diagnostic tool in adjunct to colposcopy for realizing real- time in vivo diagnosis of cervical ... autofluorescent lung tissue in vivo in realtime.39 Day et al has specially designed a miniaturized confocal endoscopic Raman probe consisting of a single illumination fiber and a single collection fiber ... by means of Raman spectroscopy during clinical colposcopy (integration time: 90 sec).33 In 2001, the group published the first in vivo clinical trial results, demonstrating the use of Raman spectroscopy...

Ngày tải lên: 10/09/2015, 09:24