reactive oxygen species as mediators of signal transduction in cardiovascular diseases
Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps
Ngày tải lên :
09/08/2014, 08:23
... versus SIN-1, p ≤ 0.05; Figure 4a) In cartilage explants, no increase in VEGF was detected at μg of PMA or μM of SIN-1 (Figure 4b) Using higher concentrations, VEGF production was increased 2.7-fold ... participating in these processes, especially the surrounding synovial tissue This reflects the findings of Felson and coworkers [27], who declared OA to be a disease involving the whole joint In summary, ... element of the VEGF promoter The mode of action of SIN-1 is presumably by an increase in HIF-1α (hypoxia inducible factor subunit alpha) Future studies are necessary to investigate the signal transduction...
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Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
Ngày tải lên :
16/09/2015, 08:31
... phosphorylation of a number of serine residues within the distal C-terminal cytoplasmic domain The major intracellular signal that leads to increase in NHE1 activity is an increase in H+ concentration ... apoptosis include a cascade of proteases termed caspases Currently 11 human caspases has been identified Initiator caspases including caspase-1, 2, 4, 5, 9, 11 and 12 interact with upstream adapter ... ROS in various cellular functions In addition, plasma membrane of many cells has another ROS-generating enzyme utilizing NADH as an electron donor Both of these flavin-containing oxidases are inhibited...
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Báo cáo y học: "Endogenous TGF-β activation by reactive oxygen species is key to Foxp3 induction in TCR-stimulated and HIV-1-infected human CD4+CD25- T cells" docx
Ngày tải lên :
13/08/2014, 05:22
... time points and then intracellular ROS was stained with DHE The mean fluorescence intensity (MFI) of DHE in a single cell was measured with FACS A aliquot of cells was stained with Annexin-V and ... induction of Tregs by TCR stimulation in human T cells In the human immune system, Tregs play an important role in regulating immune responses, as well as in controlling immune diseases such as infection ... TCRinduction of Foxp3 in human T cells is not understood Since TGF-β has been implicated in the induction of Tregs in murine cells, we set out to investigate whether TGF-β has a role in the unexpected induction...
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Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
Ngày tải lên :
16/03/2014, 06:20
... FAD-binding domain of Nox5, which increases the Ca2+ sensitivity of the Nox5 activity regulated by the N-terminal Ca2+-binding domain [73] Second, a consensus calmodulin-binding site is present in ... Rac-binding region at the N-terminus, and binding of Ca2+ to the EF-hands prevents Rac from binding to the N-terminal region, resulting in inactivation of this oxidase [76] Structural studies of ... (organelle) protein that is made up of two structural domains, each containing about 150 amino acids: the C-terminal region includes most of the residues involved in NADPH binding, whereas the large...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... activity was expressed as unitsÆmg protein)1, with unit of SOD activity being defined as the amount of proteins causing half-maximal inhibition of the rate of NBT reduction GST activity was assayed in ... cells were incubated in NaCl/Pi for 90 min, the intracellular fluorescence signal dramatically increased in all cases (Fig 9, Table 1) The increases, in comparison to the signals observed in standard ... the ROS spin-trapping N-tert-butyl-a-phenylnitrone (PBN) [47,48] Figure shows a trend of increasing aconitase activity in almost all PBN-treated cell lines The increase was most marked in bone...
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Báo cáo Y học: Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species pot
Ngày tải lên :
31/03/2014, 21:21
... addition of NADH in the presence of thenoyltrifluoroacetone (TTFA), a specific inhibitor of succinate dehydrogenase and fumarate reductase, prevented the increase in signal intensity in the g % ... by succinate [17] As a consequence, an increase in signal intensity of the reduced center I of fumarate reductase in NADH- compared to succinate-reduced membranes might occur, as shown in Fig ... according to [6] In parallel with a decrease in signal intensity of the Fe–S cluster, there was a decrease in the specific succinate dehydrogenase activity (Table 2) No succinate dehydrogenase activity...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
Ngày tải lên :
20/06/2014, 00:20
... role in the pathogenesis of several respiratory disorders, including bronchial asthma [26] In particular, increasing evidence shows that chronic airway inflammation typical of asthma results in increased ... of reactive oxygen species To measure the amount of free radicals generated, a chemiluminescence (CL) assay was performed as described by Trush [22] using a luminescence counter (20/20 n Luminometer, ... neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species by neutrophils in contact with PM2.5 A In vitro...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:20
... effect of saikosaponin was mainly through enhancing the cisplatin-induced apoptosis, which was accompanied by enhanced activation of caspase and the cleavage of caspase substrate PARP, and was blocked ... cisplatin (5-10 μM) alone or both for 48 h Cell death was measured as described in (A) (C) HeLa cells were treated with increasing concentrations of saikosaponin-d or fixed concentration of cisplatin ... cisplatin retained similar trend of O2- induction as treated by the saikosaponins alone Similar trend and more striking extent of H2O2 induction by SSa or SSd, alone or in combination with cisplatin...
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Báo cáo y học: " Early The tripeptide feG regulates the production of intracellular reactive oxygen species by neutrophils" pps
Ngày tải lên :
11/08/2014, 08:21
... a regulation of CD11a/CD18 affinity for counter ligands by a conformational switch in the I domain of this integrin [63] Since engagement of integrins contributes to increases in vascular permeability ... neutrophils Integrin expression on the cell surface of neutrophils was determined by measuring the mean fluorescence intensity (MFI) of specific antibody binding for each integrin Ovalbumin sensitized ... eotaxin and eosinophils in eosinophilic gastrointestinal inflammation Nat Immunol 2001, 2(4):353-360 Teixeira MM, Das AM, Miotla JM, Perretti M, Hellewell PG: The role of lipocortin-1 in the inhibitory...
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báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
Ngày tải lên :
12/08/2014, 03:20
... catabolism, including a number of oxidasebased processes, by soluble sugars [18,53] In contrast, atrazine by itself was found to induce a number of genes encoding oxidases, the most highly induced being ... variation in the presence of atrazine in comparison to control condition In contrast to the repressive effects of atrazine, which were associated with a decrease of DHAR activity, the increase of DHAR ... combined effects of sucrose and atrazine This strongly suggested important interactions of sucrose and xenobiotic signalling or of sucrose and ROS signalling, thus resulting in induction of specific...
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Báo cáo khoa học: "A Reactive oxygen species: toxic molecules or spark of life" docx
Ngày tải lên :
12/08/2014, 23:21
... example, in bacteria the transcription factor OxyR is redox sensitive [13] There is now an increasing number of examples in animals of ROS-based signaling, including protein tyrosine phosphatase 1B ... identification of SOD, one of the tests of the activity of the enzyme was the prevention of oxidation of catecholamines [33] The potential clinical importance of the oxidation of catecholamines was demonstrated ... quantities of O2•–, whereas the NAD(P)H oxidases in non-phagocytic cells produce much smaller amounts of O2•–, consistent with a signalling role The role of ROS in the signaling of a number of growth...
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Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx
Ngày tải lên :
12/08/2014, 23:22
... leukemia in BLV-infected sheep using the VPA histone deacetylase inhibitor [21] Since we showed that VPA acts at least in part through induction of apoptosis, we investigated the involvement of ROS in ... expression of these proteins and disruption of ROS homeostasis has been involved in the development of virus-induced malignancies [26] Intracellular overexpression of TRX was detected in cells ... using an antibody specific for the acetylated form of histone H3 Actin was analyzed in parallel as a loading control B) The extent of B cell apoptosis was measured by determining the level of...
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Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species
Ngày tải lên :
24/08/2014, 12:59
... in STAT3 KO mice is in part caused by an increase in the basal level of ROS and/or insensitivity of ROS levels in response to mechanical loading 30 In order to further investigate the role of ... protein levels markedly increase in response to mechanical loading In addition, as indicated by STAT3 phosphorylation in MC3T3-E1 osteoblastic cells, STAT3 activity significantly increases in response ... microstrains The left ulnas were not loaded, and served as internal controls Calcein was injected days after the first loading bout, and alizarin was injected days after the first loading bout These injections...
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A study to investigate the involvement of nadph oxidase 5 (NOX5) in resveratrol (RSV) induced reactive oxygen species (ROS) production in u937 cells
Ngày tải lên :
26/09/2015, 10:44
... proteinases) are a group of proteins involved in regulating cellular migration and metastasis NOX4-derived ROS is involved in the activation of MMPs that lead to regulation of genes involved in ... phase cell cycle arrest In the case of p53, it has 26 Introduction been studied that RSV increases the expression level and activity of p21 (cyclin dependent kinase inhibitor) thus resulting in ... structural proteins such as the integrins and regulatory signaling molecules such as tyrosine kinases and small G-proteins The ROS-generated by NOX present in this compartment serve as intermediate/secondary...
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Role of neutrophil NADPH oxidase derived reactive oxygen species (ROS) in innate immune responses
Ngày tải lên :
21/05/2016, 22:38
... not in this form at physiological pH values Only in a few proteins, including certain phosphatases involved in signal transduction cascades, has a cysteine located in a local environment in which ... Other cargo of this granule class include the defensins, lysozyme, bactericidal/permeability-increasing protein (BPI), and a number of serine proteases: neutrophil elastase (NE), proteinase (PR3), ... oxidase system The long lived macrophages, in contrast, restrict their production of extracellular ROS ROS are increasingly being found to be involved in both intra and intercellular signalling...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... cytokines from gastric epithelial cells resulting in the increased generation of ROS in gastric epithelial cells [24] It has been shown that Hp infection can increase the expression and activity of ... development [31] In the study of Machado et al., their results showed Hp infection resulted in increased mutations in the non-coding D-loop as well as the coding genes ND1 and COI of mtDNA of gastric cells ... cells [12] The increase in the number of mutations was mainly attributed to a rise of transitions, possibly a consequence of oxidative damage The increase in mtDNA mutations was dependent on...
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Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine fibrosarcoma L929 cells doc
Ngày tải lên :
18/02/2014, 13:20
... results indicate that oridonin induced autophagy in L929 cells Inhibition of autophagy up-regulates apoptosis in oridonin-induced L929 cells To investigate the role of autophagy in oridonininduced ... up-regulated p38 activation in oridonin-induced L929 cells Interestingly, we found that inhibition of p38 decreased oridonin-induced NF-jB activation, indicating that oridonin-induced p38 activation ... Specificity of a third kind: reactive oxygen and nitrogen intermediates in cell signaling J Clin Invest 111, 769–778 Finkel T, Serrano M & Blasco MA (2007) The common biology of cancer and aging Nature...
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Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx
Ngày tải lên :
16/03/2014, 18:20
... (v8) in the model was increasingly inhibited (by increasing the concentration of the competitive inhibitor I) This caused an increase in both the amplitude and duration of signaling Both kinase ... predicted that, in a protein kinase signaling pathway, kinases mainly control signaling amplitude, whereas phosphatases control both signaling amplitude and duration of signaling [22] Here, we shall ... second kinase exerted substantial control on signaling amplitude, both in the initial phase of signaling and Fig Time prole of the activity of the four proteins in the model The receptor R is inactivated...
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Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
Ngày tải lên :
23/03/2014, 09:21
... Neuroglobin and cytoglobin: oxygen- binding proteins in retinal neurons Invest Ophthalmol Vis Sci 47, 1016–1023 Stein I, Itin A, Einat P, Skaliter R, Grossman Z & Keshet E (1998) Translation of vascular ... Ngb in the mouse retina is > 100 lm, and is thus within the range of Mb in the muscle, indicating the important role of Ngb in retinal function [18] However, in contrast to Mb, the O2 affinity of ... protein level was studied in second order As can be seen in Fig 3, in eyes, during hypoxic incubation, H2O2 production remained at levels comparable with normoxia This finding was expected, as it...
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