reactive oxygen species and oxidative damage

... MJ: Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants Plant Physiol 2008, 148:960-968 53 Lee KP, Kim C, Landgraf F, Apel K: EXECUTER1- and EXECUTER2dependent ... R: Reactive oxygen signaling and abiotic stress Physiol Plant 2008, 133:481-489 38 Van Breusegem F, Bailey-Serres J, Mittler R: Unraveling the tapestry of networks involving reactive oxygen species ... C, Bogard M, Couee I, Gouesbet G: Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets...

Ngày tải lên: 11/08/2014, 11:22

... reductase; MS: Murashige and Skoog; NBT: nitroblue tetrazolium; O2: molecular oxygen; 1O2: singlet oxygen; O2.-: superoxide radical; PSII: photosystem II; ROS: reactive oxygen species; SOD: superoxide ... Asada K: Production and scavenging of reactive oxygen species in chloroplasts and their functions Plant Physiol 2006, 141(2):391-396 Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman J, ... family with disparate regulation and protein localization Plant Physiol 1998, 118(2):637-650 Apel K, Hirt H: Reactive oxygen species: Metabolism, oxidative stress, and signal transduction Annu Rev...

Ngày tải lên: 12/08/2014, 03:20

... and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and the nucleotide positions 16024±16 324 and ... relationship between ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95 ... 40% and is considerably lower in children and adolescents than in adults and elderly people [1] To date, the pathogenic mechanisms have not been well defined Studies have shown Hp can cause damage...

Ngày tải lên: 25/10/2012, 11:18

... regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other hand, there ... region, and NADPH-binding and FADbinding domains in the C-terminal cytoplasmic region (Fig 1A), forming a complete apparatus that transports electrons from NADPH via FAD and two hemes to molecular oxygen ... reduce Fe3+ and Cu2+ for iron and copper uptake, but fail to use molecular oxygen as a substrate [19,20] Among the conserved transmembrane segments of gp91phox ⁄ Nox2, the third and fifth helices...

Ngày tải lên: 16/03/2014, 06:20

... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in tissues ... Signorile, A., Stahl, W., Vina-Ribes, J & Astley, S.B (2002) Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function Mol Aspects Med 23, 209–285 Dunbar, D.R., ... mediated by reactive oxygen species and p38 MAPK signaling pathway in human HepG2 hepatoma cells Free Radic Biol Med 32, 1132–1142 Gardner, P.R (2002) Aconitase: sensitive target and measure...

Ngày tải lên: 16/03/2014, 18:20

... dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS-scavenging function of Ngb and on the ... levels For brain and eyes, there was a gradual increase in the amount of H2O2 after hypoxia and reoxygenation In heart, muscle and liver, the H2O2 level dropped after 24 h of reoxygenation The ... in mice under hypoxic conditions and after reoxygenation Cleaved caspase-3 protein expression in heart and liver under hypoxic (H) conditions (48 h) and after reoxygenation (48R8, 48R24: 48 h of...

Ngày tải lên: 23/03/2014, 09:21

... between mtDNA repair and degradation pathways ROS induce both single-strand and double-strand breaks in mtDNA, as well as abasic (AP) sites and base damage Both base damage and AP sites are converted ... (GPx1; Fig and Eqn 4) and peroxiredoxins III and V (PrxIII and PrxV; Fig and Eqns and 6, respectively; [55]) At least seven GPx enzymes have been described to date in mammalian cells [56], and two ... Intracellular generation of reactive oxygen species by mitochondria Biochem Pharmacol 69, 719–723 Andreyev AY, Kushnareva YE & Starkov AA (2005) Mitochondrial metabolism of reactive oxygen species Biochemistry...

Ngày tải lên: 29/03/2014, 22:21

... or damage lipids, proteins or DNA [13] In the nomenclature of free radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species ... growth factor isoforms and their receptors are expressed in human osteoarthritic cartilage Am J Pathol 2003, 162:171-181 Henrotin Y, Kurz B, Aigner T: Oxygen and reactive oxygen species in cartilage ... in biochemistry and molecular biology Edited by: Burdon RH Amsterdam: Elsevier; 1991:20 Henrotin YE, Bruckner P, Pujol JP: The role of reactive oxygen species in homeostasis and degradation of...

Ngày tải lên: 09/08/2014, 08:23

... of Analysis DNA oxidative damage Analysis of DNA oxidative damage After 24 h of culture of PBMCs from BLV-infected (n = 3) and control (n = 3) sheep, cells were stained for IgM and 8-hydroxy-2'-deoxyguanosine ... (488 nm) and HeNe (633 nm) lasers Measurement of DNA oxidative damage in B cells The extent of DNA damage resulting from oxidative stress was evaluated by quantification of the major oxidative ... François Debande and André Théwis for support in animal housing facilities, Michèle Nuttings and Daniel Portetelle for providing monoclonal antibodies and Vincenzo Ciminale for helpful comments and critiques...

Ngày tải lên: 12/08/2014, 23:22

... expression by reactive oxygen species Proc Natl Acad Sci USA 2003, 100:15035-15040 Hildeman DA, Mitchell T, Teague TK, Hanson P, Day BJ, Kappler J, Marrack PC: Reactive oxygen species regulate ... Brody AR: Asbestos-derived reactive oxygen species activate TGF-beta1 Lab Invest 2004, 84:1013-1023 Hildeman DA: Regulation of T-cell apoptosis by reactive oxygen species Free Radic Biol Med ... of ROS in TCR- and CD28stimulated CD25- T cells were dramatically upregulated and continued to increase at day (Fig 6A,B and Fig 7B) Interestingly, the increase in ROS in TCR- and CD28-stim-...

Ngày tải lên: 13/08/2014, 05:22

... electrochemical gradient (28) 1.13 ROS Reactive Oxygen Species (ROS) are a type of free radical derived from oxygen, and are produced by many bodily functions, including oxidative phosphorylation that ... patience and understanding during my unconventional graduate career, and also my committee members Dr James Marrs and Dr Julie Ji I would like to thank Kevin Zhou for his invaluable help in the lab and ... University, August 2012 Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...

Ngày tải lên: 24/08/2014, 12:59

... prevention and treatment of many diseases [2] Reactive oxygen species (ROS), a group of highly reactive molecules, including singlet oxygen, hydroxyl radicals, superoxide anion, nitric oxide and hydrogen ... production of reactive oxygen species Cancer Res 65, 948–956 12 Pan J, She M, Xu ZX, Sun L & Yeung SC (2005) Farnesyltransferase inhibitors induce DNA damage via reactive oxygen species in human ... overwhelmed by ROS and subsequent oxidative stress occurs, cell damage and cell death result [4] As a mode of type II programmed cell death, autophagy plays a major role in the degradation and recycling...

Ngày tải lên: 18/02/2014, 13:20

... procedures) Results are the mean ± standard deviations for three independent experiments 10b and endogenous reactive oxygen species significantly, by 10.382-fold and 3.212-fold, respectively 25 000 ... respiratory chain and decreased DWm and ATP production (Fig 8) AA is a known antioxidant, and interactions between AA and ROS may attenuate the oxidant effect of ROS and alleviate ROS-induced damage to ... service to our authors and readers, this journal provides supporting information supplied 10b and endogenous reactive oxygen species by the authors Such materials are peer-reviewed and may be re-organized...

Ngày tải lên: 28/03/2014, 23:20

... alginolyticus cells were performed under exclusion of dioxygen In addition, the effect of enzymes that detoxify reactive oxygen species (superoxide dismutase and catalase) on the Na+-NQR activity during ... anaerobic chamber and flushed with N2 In order to investigate the influence of dioxygen and NADH on the activity of the Na+-NQR, membrane vesicles were prepared (A) in the presence of dioxygen without ... Unemoto, with NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox...

Ngày tải lên: 31/03/2014, 21:21

... generation of reactive oxygen species in a nuclear factor kappaB-dependent manner Gastroenterology 2010, 138:2509-2518, 2518 e2501 Mendez-Samperio P, Perez A, Alba L: Reactive oxygen species- activated ... oxidase pathway and Nrf2 in human tracheal smooth muscle cells J Immunol 2008, 181:5098-5110 Lee HM, Shin DM, Kim KK, Lee JS, Paik TH, Jo EK: Roles of reactive oxygen species in CXCL8 and CCL2 expression ... blunted cytokine (TNF-a and IL-1b) and chemokine (CCL2 and CXCL10) protein production in virus-infected microglial cultures (Figure 4A-D) Viral infection activates p38 and p44/42 (ERK1/2) MAPKs...

Ngày tải lên: 19/06/2014, 22:20

... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species ... 2004, 110:1134-1139 Halliwell B, Whiteman M: Measuring reactive species and oxidative damage in vivo and in cell culture: how should you it and what the results mean? Br J Pharmacol 2004, 142(2):231-255 ... mm; PON: Paraoxonase; RNS: Reactive nitrogen species; ROS: Reactive oxygen species; S: Sulfur; SO2: Sulfur dioxide; SOD: Superoxide dismutase; TBARS: Thiobarbituric acid reactive substances; TNFa:...

Ngày tải lên: 20/06/2014, 00:20

... contributions XW and YL designed research and wrote and revised the manuscript; QW performed all research experiments and analyzed data; XLZ assisted with cell death experiment LY and YJZ assisted ... Generation of reactive oxygen species and induction of apoptosis Journal of carcinogenesis 2009, 8:9 26 Lin Y, Shi R, Wang X, Shen HM: Luteolin, a flavonoid with potential for cancer prevention and therapy ... times and the average is shown in each figure For morphological study of cell death, cells were stained with 50 μg/mL of acridine orange and 50 μg/mL of ethidium bromide and then observed and photographed...

Ngày tải lên: 10/08/2014, 10:20

... (PMN); ROS: reactive oxygen species Competing interests The author(s) declare that they have no competing interests Authors' contributions All authors participated in study design and read and approved ... tissue repair by submandibular gland factors Immunol Today 1994, 15(11):527-532 Rougeot C, Rosinski-Chupin I, Mathison R, Rougeon F: Rodent submandibular gland peptide hormones and other biologically ... intracellular oxidative burst activity of neutrophils Several PKC isozymes (α, βII, δ and ζ) are involved in the regulation of NADPH oxidase and the respiratory burst of human and rat neutrophils...

Ngày tải lên: 11/08/2014, 08:21

... glutathione, and DNA injury Besides oxygen- based radicals, there are also reactive nitrogen species such as nitric oxide (NO) and nitrogen dioxide (NO2), sulfur based molecules such as thinyl (RS) and ... calcium handling, which is essential for intracellular signaling Lipid peroxides can also damage DNA and proteins Attack of DNA by ROS results in DNA strand breaks As with lipids, O2•– and H2O2 ... was abandoned because of the hole and rusted when it was no longer cared for Signs of oxidative changes may simply indicate that molecules or cells have been abandoned by the organism and are...

Ngày tải lên: 12/08/2014, 23:21

Ngày tải lên: 14/11/2014, 06:34