reactive oxygen and nitrogen based species

... these molecules with the level and function of UCP2 and free radical production in macrophages We find that MDP induces reactive oxygen and nitrogen species production and upregulates UCP2 protein ... effect of MDP on ROS and reactive nitrogen species production by murine peritoneal macrophages + n + /An PI Fig The percentage of viable, dead and apoptotic cells in treated and untreated cells ... peritoneal macrophages by MDP increased cellular ROS and reactive nitrogen species (RNS) production The increased production of reactive species was apparent after h of stimulation Since UCP2...

Ngày tải lên: 05/03/2014, 23:20

... travels, and assure us that, after wandering about in all sorts of places, it had returned to us the same little atom of nitrogen which we had owned twenty years before, and which for thousands of ... ammonia? Ammonia is composed of nitrogen and hydrogen It has a pungent smell and is familiarly known as hartshorn The same odor is perceptible around stables and other places where animal matter ... decomposition of nitrogenous matter had sent into the atmosphere, and we find that all rain, snow and dew, contain ammonia This fact may be chemically proved in various ways, and is perceptible...

Ngày tải lên: 29/06/2014, 15:20

... and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and the nucleotide positions 16024±16 324 and ... relationship between ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95 ... activity and expression of base excision repair and mismatch repair are down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA...

Ngày tải lên: 25/10/2012, 11:18

... regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other hand, there ... region, and NADPH-binding and FADbinding domains in the C-terminal cytoplasmic region (Fig 1A), forming a complete apparatus that transports electrons from NADPH via FAD and two hemes to molecular oxygen ... reduce Fe3+ and Cu2+ for iron and copper uptake, but fail to use molecular oxygen as a substrate [19,20] Among the conserved transmembrane segments of gp91phox ⁄ Nox2, the third and fifth helices...

Ngày tải lên: 16/03/2014, 06:20

... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in tissues ... Signorile, A., Stahl, W., Vina-Ribes, J & Astley, S.B (2002) Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function Mol Aspects Med 23, 209–285 Dunbar, D.R., ... mediated by reactive oxygen species and p38 MAPK signaling pathway in human HepG2 hepatoma cells Free Radic Biol Med 32, 1132–1142 Gardner, P.R (2002) Aconitase: sensitive target and measure...

Ngày tải lên: 16/03/2014, 18:20

... dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS-scavenging function of Ngb and on the ... levels For brain and eyes, there was a gradual increase in the amount of H2O2 after hypoxia and reoxygenation In heart, muscle and liver, the H2O2 level dropped after 24 h of reoxygenation The ... in mice under hypoxic conditions and after reoxygenation Cleaved caspase-3 protein expression in heart and liver under hypoxic (H) conditions (48 h) and after reoxygenation (48R8, 48R24: 48 h of...

Ngày tải lên: 23/03/2014, 09:21

... (GPx1; Fig and Eqn 4) and peroxiredoxins III and V (PrxIII and PrxV; Fig and Eqns and 6, respectively; [55]) At least seven GPx enzymes have been described to date in mammalian cells [56], and two ... Intracellular generation of reactive oxygen species by mitochondria Biochem Pharmacol 69, 719–723 Andreyev AY, Kushnareva YE & Starkov AA (2005) Mitochondrial metabolism of reactive oxygen species Biochemistry ... apyrimidinic endonuclease APE ⁄ Ref1; SSB and DSB, single-strand break and double-strand break, respectively ase, may aid in the generation of abasic sites and single-strand breaks This model provides a...

Ngày tải lên: 29/03/2014, 22:21

... radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species and other oxidant species The relationship between ROS and articular ... growth factor isoforms and their receptors are expressed in human osteoarthritic cartilage Am J Pathol 2003, 162:171-181 Henrotin Y, Kurz B, Aigner T: Oxygen and reactive oxygen species in cartilage ... in biochemistry and molecular biology Edited by: Burdon RH Amsterdam: Elsevier; 1991:20 Henrotin YE, Bruckner P, Pujol JP: The role of reactive oxygen species in homeostasis and degradation of...

Ngày tải lên: 09/08/2014, 08:23

... R: Reactive oxygen signaling and abiotic stress Physiol Plant 2008, 133:481-489 38 Van Breusegem F, Bailey-Serres J, Mittler R: Unraveling the tapestry of networks involving reactive oxygen species ... species and reactive nitrogen species by angiosperm stigmas and pollen: potential signalling crosstalk? New Phytologist 2006, 172:221-228 43 Potocky M, Jones MA, Bezvoda R, Smirnoff N, Zarsky V: Reactive ... MJ: Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants Plant Physiol 2008, 148:960-968 53 Lee KP, Kim C, Landgraf F, Apel K: EXECUTER1- and EXECUTER2dependent...

Ngày tải lên: 11/08/2014, 11:22

... reductase; MS: Murashige and Skoog; NBT: nitroblue tetrazolium; O2: molecular oxygen; 1O2: singlet oxygen; O2.-: superoxide radical; PSII: photosystem II; ROS: reactive oxygen species; SOD: superoxide ... Asada K: Production and scavenging of reactive oxygen species in chloroplasts and their functions Plant Physiol 2006, 141(2):391-396 Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman J, ... hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo Plant J 2007, 50(3):475-487 Chao L, Zhou QX, Chen S, Cui S, Wang ME: Single and joint stress...

Ngày tải lên: 12/08/2014, 03:20

... epithelial cells (RLE), reactive oxygen and nitrogen species (ROS/RNS), redox Acknowledgements The study was financially supported by the Ministerium of Wirtschaft, Mittelstand, Technologie und ... and para-aramid fibrils IARC Press, Geneva, Switzerland; 1997 Donaldson K, Borm PJA: The quartz hazard: a variable entity Ann Occup Hyg 1998, 42:287-294 Fubini B, Hubbard A: Reactive oxygen species ... 42:287-294 Fubini B, Hubbard A: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis Free Radic Biol Med 2003, 34:1507-1516 Begin R,...

Ngày tải lên: 12/08/2014, 18:20

... François Debande and André Théwis for support in animal housing facilities, Michèle Nuttings and Daniel Portetelle for providing monoclonal antibodies and Vincenzo Ciminale for helpful comments and critiques ... BLVinfected and control sheep were cultivated in the absence and the presence of mM VPA (Sigma/Aldrich) and the percentages of apoptotic B cells were determined as described above Analysis of reactive oxygen ... Venerando A, Cavallari I, Petronilli V, La Rocca N, Marin O, Chieco-Bianchi L, Di Lisa F, D'Agostino DM, Bernardi P, Ciminale V: Modulation of mitochondrial K(+) permeability and reactive oxygen species...

Ngày tải lên: 12/08/2014, 23:22

... expression by reactive oxygen species Proc Natl Acad Sci USA 2003, 100:15035-15040 Hildeman DA, Mitchell T, Teague TK, Hanson P, Day BJ, Kappler J, Marrack PC: Reactive oxygen species regulate ... Brody AR: Asbestos-derived reactive oxygen species activate TGF-beta1 Lab Invest 2004, 84:1013-1023 Hildeman DA: Regulation of T-cell apoptosis by reactive oxygen species Free Radic Biol Med ... of ROS in TCR- and CD28stimulated CD25- T cells were dramatically upregulated and continued to increase at day (Fig 6A,B and Fig 7B) Interestingly, the increase in ROS in TCR- and CD28-stim-...

Ngày tải lên: 13/08/2014, 05:22

... UNIVERSITY GRADUATE SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science ... patience and understanding during my unconventional graduate career, and also my committee members Dr James Marrs and Dr Julie Ji I would like to thank Kevin Zhou for his invaluable help in the lab and ... University, August 2012 Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...

Ngày tải lên: 24/08/2014, 12:59

... prevention and treatment of many diseases [2] Reactive oxygen species (ROS), a group of highly reactive molecules, including singlet oxygen, hydroxyl radicals, superoxide anion, nitric oxide and hydrogen ... production of reactive oxygen species Cancer Res 65, 948–956 12 Pan J, She M, Xu ZX, Sun L & Yeung SC (2005) Farnesyltransferase inhibitors induce DNA damage via reactive oxygen species in human ... antioxidant by selectively reducing cytotoxic oxygen radicals Nat Med 13, 688–694 Nathan C (2003) Specificity of a third kind: reactive oxygen and nitrogen intermediates in cell signaling J Clin...

Ngày tải lên: 18/02/2014, 13:20

... procedures) Results are the mean ± standard deviations for three independent experiments 10b and endogenous reactive oxygen species significantly, by 10.382-fold and 3.212-fold, respectively 25 000 ... service to our authors and readers, this journal provides supporting information supplied 10b and endogenous reactive oxygen species by the authors Such materials are peer-reviewed and may be re-organized ... generation in C albicans SC5314 cells without and with 10b and MCZ The concentrations of 10b and MCZ were 1, and lgÆmL)1 ROS levels represent the mean ± standard deviations for three independent experiments...

Ngày tải lên: 28/03/2014, 23:20

... alginolyticus cells were performed under exclusion of dioxygen In addition, the effect of enzymes that detoxify reactive oxygen species (superoxide dismutase and catalase) on the Na+-NQR activity during ... anaerobic chamber and flushed with N2 In order to investigate the influence of dioxygen and NADH on the activity of the Na+-NQR, membrane vesicles were prepared (A) in the presence of dioxygen without ... Unemoto, with NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox...

Ngày tải lên: 31/03/2014, 21:21

... generation of reactive oxygen species in a nuclear factor kappaB-dependent manner Gastroenterology 2010, 138:2509-2518, 2518 e2501 Mendez-Samperio P, Perez A, Alba L: Reactive oxygen species- activated ... oxidase pathway and Nrf2 in human tracheal smooth muscle cells J Immunol 2008, 181:5098-5110 Lee HM, Shin DM, Kim KK, Lee JS, Paik TH, Jo EK: Roles of reactive oxygen species in CXCL8 and CCL2 expression ... blunted cytokine (TNF-a and IL-1b) and chemokine (CCL2 and CXCL10) protein production in virus-infected microglial cultures (Figure 4A-D) Viral infection activates p38 and p44/42 (ERK1/2) MAPKs...

Ngày tải lên: 19/06/2014, 22:20

... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species ... hypochlorous acid (HOCl) and reactive nitrogen species (RNS) also increases, resulting in the oxidation of tyrosine and nitrite and subsequent formation of tyrosyl and nitrogen dioxide (.NO2) ... mm; PON: Paraoxonase; RNS: Reactive nitrogen species; ROS: Reactive oxygen species; S: Sulfur; SO2: Sulfur dioxide; SOD: Superoxide dismutase; TBARS: Thiobarbituric acid reactive substances; TNFa:...

Ngày tải lên: 20/06/2014, 00:20

... of light, water and species on leaf CT, N and growth under a fixed level of nutrients (N–) The sources of variation were light levels (L8, L36), water levels (W500, W800) and species (Q ilex, ... of light, nutrients and species on leaf CT, N and growth under a fixed level of water (W800) The sources of variation are light levels (L8, L36), nutrient levels (N−, N+) and species (Q ilex, Q ... different light, nutrients and watering treatments factorially combined Each combination of light, water and nutrients availability included 21 seedlings per species and was repeated in three...

Ngày tải lên: 07/08/2014, 16:21