reaction of melatonin with reactive oxygen species

... hypoxia 48R8, 48R24: 48 h of hypoxia, followed by h or 24 h of reoxygenation be the reaction with superoxide to form peroxynitrite In the Fe2+–NO form, Ngb reacts more rapidly with ONOO– than does ... the reaction of met(Fe3+) Ngb with ONOO– or H2O2 does not appear to generate the cytotoxic ferryl (Fe4+) species, which results in the formation of secondary protein radicals This feature of Ngb ... (48 h) and after reoxygenation (48R8, 48R24: 48 h of hypoxia, followed by h or 24 h of reoxygenation) Positive control: U937 monocytes treated with 50 lM etoposide for h such as reactive thiols,...

Ngày tải lên: 23/03/2014, 09:21

... Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science Choose your degree I certify that in the preparation of this ... through regulation of nuclear genes, inactivation of STAT3 is shown to disrupt mitochondrial activities and result in an increased level of reactive oxygen species (ROS) Inactivation of STAT3 suppressed ... University, August 2012 Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...

Ngày tải lên: 24/08/2014, 12:59

... Control % of 8-OHdG positive B cells Red CM-XROS 60 Profile of fluorescence intensities 50 40 30 20 10 Control Figure of Analysis DNA oxidative damage Analysis of DNA oxidative damage After 24 h of ... demonstrated in this report that direct inhibition of apoptosis by bovine leukemia virus correlates with reduced production of reactive oxygen species ex vivo, a mechanism that implicates the thioredoxin ... mixed with EDTA used as an anticoagulant (1 ml of 7.5% EDTA per 25 ml of blood) PBMCs were washed twice with phosphate-buffered saline (PBS) supplemented with 0.075% EDTA and three time with PBS...

Ngày tải lên: 12/08/2014, 23:22

... during the reaction of nitrite with carp hemoglobin at different oxygen saturations (A) Reaction of nitrite with deoxyHb (oxygen saturation = 2%) (B) Reaction of nitrite with hemoglobin with an ... The reactions of nitrite with oxygenated hemoglobin (oxyHb) and deoxygenated hemoglobin (deoxyHb) are very different, and it is only the reaction with deoxyHb that produces NO The reaction of ... Instantaneous reaction rates for the reaction of nitrite with deoxygenated and oxygenated carp hemoglobin at different oxygen saturations in the absence (A, C) and presence (B, D) of ATP Reaction...

Ngày tải lên: 16/03/2014, 06:20

... 3-phosphate moiety of the bound PtdIns(3)P, NH2 and NE in the side chain of this residue forming hydrogen bonds with oxygens of the 3-phosphate; NH1 and NH2 of Arg105 form hydrogen bonds with the 4-OH ... conserved in the C-terminal region of Noxo1, which is capable of interacting with p67phox as well as with Noxa1 [106] The interaction of the Noxa1 SH3 domain with the Noxo1 PPR (Fig 7B) plays ... Fig 13 Interaction of the p67phox C-terminal SH3 domain with the p47phox C-terminus (A) Ribbon diagram of the structure of the complex of the p67phox C-terminal SH3 domain with the p47phox C-terminal...

Ngày tải lên: 16/03/2014, 06:20

... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in ... modification of the procedure of Anderson, as described [31,32] The assay is based on the determination of a chromophoric product, 2-nitro-5-thiobenzoic acid, resulting from the reaction of 5,5¢-dithiobis-(2-nitrobenzoic ... derivatization with 2-vinylpiridine at room temperature for 60 In a typical experiment, 0.15 mL of supernatant was treated with lL of undiluted 2-vinylpyridine Nine microliters of triethanolamine...

Ngày tải lên: 16/03/2014, 18:20

... flushed with N2 In order to investigate the influence of dioxygen and NADH on the activity of the Na+-NQR, membrane vesicles were prepared (A) in the presence of dioxygen without additions (B) with ... proteolytic fragment of the FrdA subunit [19] The stoichiometry and localization of the flavin cofactors of the Na+-NQR are a matter of debate [7,20] A contamination of the Na+-NQR with FrdA that contains ... [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox cofactors in membranes from V alginolyticus In the presence of NADH and dioxygen, the purified Na+NQR from...

Ngày tải lên: 31/03/2014, 21:21

... oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species by neutrophils in contact with PM2.5 A In vitro production of reactive oxygen and nitrogen species by neutrophils ... performed using the GraphPad Prism software (version 5.0 for Windows; GraphPad Software Inc., La Jolla, CA) Quantification of reactive oxygen species To measure the amount of free radicals generated, ... Particulate matter with aerodynamic diameter < 10 mm; PM2.5: Particulate matter with aerodynamic diameter < 2.5 mm; PON: Paraoxonase; RNS: Reactive nitrogen species; ROS: Reactive oxygen species; S:...

Ngày tải lên: 20/06/2014, 00:20

... role of reactive oxygen species in homeostasis and degradation of cartilage Osteoarthritis Cartilage 2003, 11:747-755 Panasyuk A, Frati E, Ribault D, Mitrovic D: Effect of reactive oxygen species ... level of VEGF is the activation of AP-1 (activator protein 1), with subsequent activation of the TPA (12-O-tetradecanoylphorbol-13-acetate) element of the VEGF promoter The mode of action of SIN-1 ... the nomenclature of free radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species and other oxidant species The relationship...

Ngày tải lên: 09/08/2014, 08:23

... SA, Lin Y: A critical role of luteolininduced reactive oxygen species in blockage of tumor necrosis factoractivated nuclear factor-kappaB pathway and sensitization of apoptosis in lung cancer ... types of human cancer cells to cisplatin-induced cell death Importantly, we found that the chemosensitization effect of saikosaponin is mainly mediated by the induction of cellular reactive oxygen ... cells, or SKOV3 cells were treated with cisplatin or 10 μM of saikosaponin-a or μM of saikosaponin-d or combination of saikosaponin and cisplatin for 48 h The dose of cisplatin is 30 μM for Siha,...

Ngày tải lên: 10/08/2014, 10:20

... allergic reactions Since activation of neutrophils is associated with an increase in respiratory burst with the generation of ROS, an expectation is that feG, as a potent suppressor of several ... marker of cell viability, the number of neutrophils were calculated To evaluate the role of PKC on the release of superoxide leukocytes (1 × 106/ml) were preincubated for at 37°C with one of several ... Phagocytosis following translocation of the neutrophil b-cytochrome from the specific granule to the plasma membrane is associated with an increased leakage of reactive oxygen species Scand J Immunol 1992,...

Ngày tải lên: 11/08/2014, 08:21

... primary events of 1O2 06 0$ 6$ KRXUV KRXUV KRXUV KRXUV Figure Visualization of singlet oxygen detected with the SOSG fluorescent probe Visualization of singlet oxygen detected with the SOSG fluorescent ... coherent with inhibition of photosynthetic activity and of the Mehler reaction, whereby superoxide radical is formed by reduction of oxygen at the PSI site [52] Atrazine binding to D1 protein of PSII ... inhibition of electron transfer through PSI, was associated with a general repression of transcripts encoding the different isoenzymes of SOD and with a decrease of the global activity of this O2....

Ngày tải lên: 12/08/2014, 03:20

... formation of adrenochromes, which are toxic themselves Of interest, in the first identification of SOD, one of the tests of the activity of the enzyme was the prevention of oxidation of catecholamines ... evaluation of the utility of anti-oxidant therapies What is the reactive oxygen species that is causing the oxidative injury? What is the target molecule? What is the source of the ROS? What types of ... random destructive species but regulators of metabolic processes and part of the chemistry of life [10,11] Furthermore, evidence of oxidative injury may be the end result of the inflammatory...

Ngày tải lên: 12/08/2014, 23:21

Ngày tải lên: 14/11/2014, 06:34

... Apoptosis Fig C Mitochondial pathway of Apoptosis Fig D Wide array of functions attributed to Reactive Oxygen Species Fig E A model of ROS-mediated regulation of Apoptosis 15 Fig F Various plasma ... modifications of many of the proteins involved in apoptotic machinery (Dechao L et al 2004; John JH, 2004; Irani K and Pascal JGC, 1998) I.2 Reactive Oxygen Species and Apoptosis Cells generate reactive oxygen ... transport H+ out of the cell in exchange for Na+ with a stoichiometry of 1:1 In mammalian cells, the NHE family consists of nine isoforms, NHE-1 to NHE-9 Whereas most of the isoforms are restricted...

Ngày tải lên: 16/09/2015, 08:31

... Modulation of cellular redox status 26 (iv) Autophagy and metabolism (v) RSV and Reactive oxygen species (ROS)/NADPH Oxidase (NOX) 1.5: Aim of study 29 1.6: Mode of action of ... of ROS species NOX enzymes are one of the major sources of ROS NOX5 belongs to the family of NOX enzymes NOX5 differs from the other members of the NOX family in aspects as such as its mode of ... biochemical reactions involved in the formation of Reactive oxygen species (ROS) FIGURE 2: Diagram illustrating the importance of cellular homeostatic redox balance FIGURE 3: The intracellular sources of...

Ngày tải lên: 26/09/2015, 10:44

... reduction of O2 to water without the formation of ROS However, partial reduction of O2, which results in the generation of ROS, can occur if O2 interacts with the electron transport chain upstream of ... ROS? ROS include a number of chemically reactive partially reduced forms of oxygen including O2−, H2O2, and HO• These ROS are substantially more reactive than molecular oxygen which is itself a ... dissertation List of tables List of tables Table List of antibodies used for flow cytometry analysis 20 Doctoral dissertation Summary SUMMARY I have investigated the role played by reactive oxygen species...

Ngày tải lên: 21/05/2016, 22:38

... Role of oxygen- derived free radicals in Helicobacter pylori water extract-induced mouse skin carcinogenesis Biofactors 2006;28: 45-52 Shi GY, Gao F, Shi XG, et al Detection of cellular reactive oxygen ... microliters of extracts were mixed thoroughly with 20 μl of loading buffer, which were then boiled for Ten microliters of the mixture were subjected to SDS–PAGE, and bands were captured Treatments of ... Hp11638M When compared with the mtDNA of AGS cells without treatment, only three heteroplasmic mutations in the Cytb gene were found in the mtDNA of AGS cells treated with 960 μg/ml Hp11638 extract...

Ngày tải lên: 25/10/2012, 11:18

Ngày tải lên: 07/06/2013, 01:26

... required for the prevention and treatment of many diseases [2] Reactive oxygen species (ROS), a group of highly reactive molecules, including singlet oxygen, hydroxyl radicals, superoxide anion, ... washing twice with NaCl ⁄ Pi, the cells were incubated with mL of PI staining solution (50 mgÆL)1 of PI and gÆL)1 of RNase A) at °C for 30 The percentage of cells at different phases of the cell ... endogenous production of reactive oxygen species Cancer Res 65, 948–956 12 Pan J, She M, Xu ZX, Sun L & Yeung SC (2005) Farnesyltransferase inhibitors induce DNA damage via reactive oxygen species in human...

Ngày tải lên: 18/02/2014, 13:20