ranking differentially expressed genes in oa bone
Báo cáo y học: "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling" pps
... imaging system There was a significant (30%) increase in safranin O staining intensity in the patella on day When the data of all time points were pooled, a significant increase in safranin O ... stained immunohistochemically for VDIPEN (Figure 1i–l) BMP-2 initially induced an increase in VDIPEN staining on day in patellar cartilage In tibial cartilage, elevated levels of VDIPEN staining ... VDIPEN staining (k) and NITEGE staining (q,p) Arrows point to intense staining around chondrocytes FastG, fast green; SafO, safranin O Co-incubation of several dilutions of BMP-2 protein with the...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
... The initiator caspase group includes caspase-2, caspase-8, caspase-9, and caspase-10, having long NH2-terminal prodomains, which interact with adapter molecules to form a death-inducing signaling ... main well-characterized mechanism is the inhibition of active Int J Med Sci 2005 2(1) 34 caspases by the direct binding of IAPs In the many case, for example, in the lung carcinoma cells, the interaction ... HBV infection might alter the cIAPs gene expressing of liver cell, finally instigating the carcinogenesis of the cells it infected This is agreement with the observation that the patients infected...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... ultimate goal, there are some issues to consider when examining clinical tissues with DNA microarrays or qPCR First, tissues Page of 12 contain varying amounts of contamination from neighboring stromal ... After eliminating low-expressing genes, we calculated the expression ratio of each gene in each cancer cell line relative to the normal cell line From this, we identified 18 differentially expressed ... The 86-2 cell line (LC) was different in PC3 from the SC cell lines Subtypes were characterized by PC1-3 and loading number In Figure 2(D), which shows the loading number for genes, PC1 was negatively...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
... vector bearing the potential miR-23a binding site in the fluorescent reporter, and found an increase in EGFP intensity after blocking miR-23a and a decrease in EGFP intensity after overexpressing miR-23a ... elevate the EGFP intensity in the reporter vector containing a mutation of the first miR-23a binding region, but could still elevate the EGFP intensity in the reporter vectors containing mutations ... adenocarcinoma cell line MGC803 by targeting directly the interleukin-6 receptor (IL6R) gene product Results miR-23a is overexpressed in gastric adenocarcinoma An oligonucleotide microarray was...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... maintained in HO-2– ⁄ – mice through appropriate resetting of HO-1 expression In contrast with HO-1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines ... cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma) YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium (Sigma), and K562, KG1 and Jurkat cells were maintained in RPMI-1640 ... of HO-1 Incidentally, the three cell lines with low HO-1 expression, K562, Jurkat, and H146, were maintained in suspension culture (Fig 1), although HO-1 is expressed in two other cell lines, YN-1...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... [27,34] were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM) (Sigma) HeLa and HepG2 cells were maintained in DMEM Each medium contains 10% heat-inactivated FBS, penicillin G Total RNA ... and the intensity representing HO-1 or HO-2 protein was normalized with respect to the intensity for the a-tubulin signal Shown are the relative expression levels of HO-1 and HO-2 proteins in YN-1 ... expression in MEL mouse erythroleukemia cells [32] Moreover, heme induces the expression of the a-globin gene in K562 human erythroleukemia cells [33] and the number of hemoglobin-producing cells in...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... in iron-rich media We have also shown that the increase in the prooxidant state caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein ... further increase in ROS production as determined by an increase in DCFH mean fluorescence intensity (Fig 2B, thick line) In addition, determin- Fig Regulation of iron related parameters by iron loading ... threefold the ferritin content as determined by the increase in mean fluorescence intensity (MFI) (Fig 1C) As shown in Fig 1B, permeabilization with saponin did not increase background staining when rabbit...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... sequence, increasing the number of basic residues of lysine, arginine and histidine, could occur However it seemed probable that the eEF1A isoforms could act as a controlling event in maintaining the ... Eukaryotic EF1A is a protein belonging to the GTPbinding elongation factor family, which promotes the GTPdependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and ... oligomer in a buffer containing 25% glycerol, as described in the Materials and methods After incubation at room temperature for 30 min, the samples were loaded onto a 7% polyacrylamide gel in Tris/...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... alteration in PKB protein was similar to that in PKB activity (Fig 7), suggesting that the insulininduced PKB protein was almost fully phosphorylated and activated Whether insulin was internalized...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... value in Table 1) is taken as 100% (B) Basal incorporation of BrdU into proliferating LXF-289 cells in the presence of the inhibitors for the kinases calcium ⁄ calmodulin-dependent protein kinase ... kinase ⁄ (ERK1 ⁄ 2), p38 mitogen-activated protein kinase (MAPK), protein kinase C and PI3 kinase All values are means ± SD of 3–6 independent experiments run in triplicate (A) B 10 cisplatin, ... ERK-activating kinase (MEK1 ⁄ 2) (20 lm PD98059, lm U0126), p38 MAPK (20 lm SB203580) or phosphatidylinositol-3-kinase (PI3K) (wortmannin), indicating that each pathway seems to be involved in ATP-mediated...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... (all from PharMingen) Anti-(b-actin) Ig was used to confirm equal sample loading (1 : 2000; Oncogene Research Products) Following a washing step in 0.1% (v/v) Tween in Tris-buffered saline (Sigma; ... that CDO alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti-Cdt1 and anti-geminin Ig See Materials ... operate in G0 Geminin is believed to act as a cell cycle inhibitor, with a role to prevent untimely licensing by specifically binding to Cdt1 and inhibiting its MCM loading function [12,15,16] During ... differences in relative amounts of Cdt1 and its inhibitor in different cell lines It would be interesting to investigate whether geminin and/or Cdt1 levels or the ratio of Cdt1 to its inhibitor geminin...
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Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... 30 32P-Loading was further continued for 120 in the presence of protein-kinase inhibitors or proteinphosphatase inhibitors Cells were then rinsed with NaCl/Pi and disrupted with 1% NP-40 in 50 ... digested away in the absence of proteasome inhibitors (lane in Fig 1B, 32P vs WB) Inhibition of TPH degradation in the cell-free proteasome system by protein kinase inhibitors We examined the involvement ... were then disrupted with 1% NP-40 in 50 mM Tris/HCl (pH 7.8) containing the protease inhibitor cocktail (32P): In order to examine 32P-incorporation into proteins, the cell extracts were subjected...
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Báo cáo Y học: The SK-N-MC cell line expresses an orexin binding site different from recombinant orexin 1-type receptor pptx
... for orexin B 18–28 serine and the C-terminal methionine The conformational in uence of the C-terminal part of the orexins remains unclear The introduction of a helix inducers into C-terminal peptide ... of orexin A via the lateral hypothalamus and after administration of orexin A 23–33 and orexin A (A) Food intake after administration of orexin A 23–33 (B) Sequencing To study orexin binding we ... Germany) using Rink Amide resin (30 mg, resin loading 0.6 mmolÆg)1) Amino acids were attached by the Fmoc-strategy in a double coupling procedure, using a 10-fold excess of Fmoc-amino acid, HOBt...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... key amino acid in the legumain-binding site of cystatin C [22] The amino acid substitutions reside in opposing parts of the cystatin C molecule, which makes these protein variants interesting Experiments ... convincingly demonstrate that the cysteine protease inhibitor cystatin C is internalized in all five of the cancer cell lines investigated in this study Using confocal microscopy, western blotting ... fluorescence had increased in all five strains The pattern of internalization was more or less equal in the five strains Two unrelated proteins acting as protein inhibitors, potato carboxypeptidase inhibitor...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... temperature of annealing, and frequently different coexisting competing forms In particular GRO29A needs 56 h annealing at 60 °C in 140 mm KCl to assume a G-quartet containing structure [26] In agreement ... taking into account the different binding ability vs the same cytotoxicity Although a different intracellular localization could explain differences in antiproliferative effect, the protein binding ... protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer Therefore, a fixed aliquot of the protein was incubated with ng of the indicated probes After 30 incubation...
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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
... glycoproteins, respectively, when the incubation was performed in the presence of 20 lM kifunensine after a 20 preincubation G1M9 indicates oligomannosides containing one Glc, nine Man, and two GlcNAc residues ... containing one Glc, nine Man, and two GlcNAc residues M4, M5, M8 and M9 indicate oligomannosides containing four, five, eight and nine Man residues and two GlcNAc residues, respectively X indicates ... Glc1Man5GlcNAc2/Glc1Man9GlcNAc2 obtained with the glycoprotein pattern This indicates that, when glycoproteins bearing Man9 and Man5 are synthesized in the same cell line, those bearing Man5 are more efficiently...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... ROS spin-trapping N-tert-butyl-a-phenylnitrone (PBN) [47,48] Figure shows a trend of increasing aconitase activity in almost all PBN-treated cell lines The increase was most marked in bone q+ ... cells were incubated in NaCl/Pi for 90 min, the intracellular fluorescence signal dramatically increased in all cases (Fig 9, Table 1) The increases, in comparison to the signals observed in standard ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Prohaska, J.R & Ganther, H.E (1976) Selenium and glutathione peroxidase in developing rat brain J Neurochem...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... microdomains contain, among others, a potential regulatory protein of T cell growth, CD48 A pivotal role of cholesterol in maintaining such transient protein assemblies, including also HLA glycoproteins, ... chains with lipid rafts (containing CD48) may have several functional consequences in T cells First, rafts may concentrate the a chains (CD25) in the vicinity of signaling IL-2R b and cc chains, ... glycosylphosphatidylinositolanchored proteins inhibits T cell growth but not function Int Immunol 11, 1381–1393 Marmor, M.D & Julius, M (2001) Role of lipid rafts in regulating interleukin-2 receptor signaling...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... attached monolayers in DMEM containing 10% fetal bovine serum Cells were harvested by treatment with trypsin and counted every 24 h in triplicate (D) Single-cell suspensions containing 10 000 cells ... Medical College (Beijing, China) and maintained in our laboratory HEK293T (American Type Culture Collection, Manassas, VA, USA) and MCF-7 cells were maintained in 10% fetal bovine serum-supplemented ... ectopic increase in either synthesized or vector -expressed miR-22 in MCF-7 led to an 50% reduction in ERa protein levels (Fig 2E) Conversely, ERa expression was significantly elevated by inhibiting...
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