random amplification of polymorphic dna

... eht yb desuac raef dna ,]23,61[ gnildnah yb noissergga lacisyhp dna gnituohs dna ,]83,92,02[ slamina rof ytlevon a si nep gnildnah ehT sserts lanoitidda esopmi ]32,71,61,9[ gnildnah gnirud elpoep ... ,641-321.pp erafleW laminA rof snoitacilpmI dnA selpicnirP cisaB :ssertS slaminA fo ygoloiB ehT ).sde( AJ hcneM ,PG grebroM :nI sserts fo tnemerusaem ffo-sdnah dna no-sdnaH RL ,swehttaM ,RJ margnI ... mures ,h rof sserts tniartsnoc gniylppa retfa ro erofeb dna metsys gnideef eht ot detsujda dah slamina retfa yad tnemirepxe ht13 eht nO seitreporp gnixaleroim dna ,cityloixna ,evitades sah dna )HTCA(...

Ngày tải lên: 07/08/2014, 18:21

... RNA Effect of incubation temperature and time on DNA degradation and RT-PCR amplification of RNA The effect of temperature on DNA degradation and RNA amplification in the presence of UNG was ... cycles of amplification may provide positive amplification of target RNA in samples with very low concentrations Conclusion Quantitative assessment of the effect of UNG on DNA degradation and RNA amplification ... success of this approach for elimination of contaminating amplicon DNA depends on the availability of a heat-labile UNG enzyme, the heat-inactivation of which prevents cleavage of product DNA amplified...

Ngày tải lên: 19/06/2014, 08:20

... RNA Effect of incubation temperature and time on DNA degradation and RT-PCR amplification of RNA The effect of temperature on DNA degradation and RNA amplification in the presence of UNG was ... cycles of amplification may provide positive amplification of target RNA in samples with very low concentrations Conclusion Quantitative assessment of the effect of UNG on DNA degradation and RNA amplification ... success of this approach for elimination of contaminating amplicon DNA depends on the availability of a heat-labile UNG enzyme, the heat-inactivation of which prevents cleavage of product DNA amplified...

Ngày tải lên: 20/06/2014, 04:20

... sensitive detection of C trachomatis EB DNA in combination with all DNA extraction methods applied None of the other amplification systems allowed higher sensitivity than PCR regardless of the extraction ... results We therefore addressed the question of how detection limits of chlamydial DNA might change after storage of DNA depending on the different DNA extraction methods applied To our knowledge, ... of each serial dilution was performed (n = 5) for each DNA extraction method and amplification system evaluated The range of detection limits of each method is given in brackets, median (M) of...

Ngày tải lên: 12/08/2014, 11:22

... phương pháp RAPD (PCR) II Nguyên lý: _ Về kỹ thuật RAPD thực theo ba bước: Tách chiết DNA tổng số, nhân DNA máy PCR; điện di gel agarose gel polyacrylamid; xác định tính đa dạng di truyền phần ... primer đơn gắn vào hai điểm khác hai mạch đơn đối diện DNA khuôn Nếu điểm gắn primer nằm khoảng nhân (thường 200 - 2000 nucleotide) đoạn DNA nhân lên _ Sự có mặt sản phẩm quan sát thông qua điện ... chứng tỏ có tương đồng hoàn toàn hay phần DNA genome với primer oligonucleotide Các primer dùng RAPD có kích thước ngắn nên dễ tìm đoạn tương đồng mạch đơn DNA genome Vì thế, phương pháp có hiệu...

Ngày tải lên: 06/01/2015, 21:51

... are suggestive of DNA damage as a cause of NS1-induced apoptosis ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of DNA common to ... cellular DNA, and that this damage leads to apoptosis Upon detection of DNA damage, DNA damage response proteins inhibit the cell cycle and are capable of inducing apoptosis if the DNA lesion ... with 5-aminoisoquinolinone The importance of DNA damage to NS1-induced apoptosis is shown by the abrogation of apoptosis in 93 the presence of inhibitors of DNA damage recognition enzymes Both the...

Ngày tải lên: 25/10/2012, 11:18

... the roles of these DHSs on the transcription of Ig-b gene and on the maintenance of the active chromatin state Results Generation of DT40/Cre cells with a long deletion in one allele of the Ig-b ... region II Isolated nuclei were treated with DNase I for at 20 °C Concentration of DNase I for treatment of nuclei from left to right: 75, 50, 25, UÆmL)1 The DNA was purified from nuclei and digested ... generally sensitive to DNaseI, presence of cell type-specific DHSs [7,8], and active-type modification of core histone tails [9–11] From the earliest stage of the study [6], DNase I general sensitivity...

Ngày tải lên: 19/02/2014, 16:20

... reagent might also be useful in investigating the rate of turnover of mtDNA and in the derivation of mtDNA-free (q°) clones of the large number of cell lines where the classical treatment with ethidium ... addition of a TPP-moiety has been used to drive the selective uptake of a wide variety of Ó FEBS 2003 Fig Selective uptake of mitoDC-81 by mitochondria and subsequent alkylation of mtDNA The uptake of ... electrotransfer showed that the extent of alkylation was proportional to the amount of DNA present and was independent of the size of the DNA fragment (Fig 3A, lane 1) As mtDNA in vivo is negatively supercoiled...

Ngày tải lên: 20/02/2014, 11:20

... phenol/chloroform/isoamyl alcohol (25 : 24 : 1) extraction followed by addition of lL of 5-M NaCl and precipitation of DNA using mL of cold ethanol After centrifugation at 13 800 g for 30 min, the DNA ... inhibition of DNA alkylation, S23906-1 (50 lM) was first incubated with an excess of GSH or derivatives (400 lM) in 20 lL of ammonium acetate for h at 37 °C prior to the addition of the radiolabeled DNA ... further of mild agitation, and finally 200 lL of 0.1 M EDTA, pH 7.5, was added and the mixture incubated for h at 37 °C After addition of 80 lL of M NaCl, the DNA was extracted using mL of phenol/chloroform/isoamyl...

Ngày tải lên: 20/02/2014, 11:20

... PolC and DnaE The ability to bind single-stranded DNA has indeed been demonstrated for the E coli DnaE OB domain [12,16] The very N-terminal region of PolC and the C-terminal domain of DnaE appear ... probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of the so-called type II KH fold [21] One of their major differences from classical type II KH domains is the absence of the ... higher similarity to domain I of the initiator of chromosomal replication DnaA (DnaA-I) 3114 What might the function of these PolC N-terminal domains be? The involvement of related structures in protein–protein...

Ngày tải lên: 05/03/2014, 23:20

... error of the given values of Tm did not exceed ±0.2 °C The relative error of the given values of DHcal did not exceed ±10% G-actin Results Effects of the D-loop cleavage on the thermal unfolding of ... monitored with the DNase I-inhibition assay stabilities of actin isoforms Eur J Biochem 267, 476–486 24 Perieteanu AA & Dawson JF (2008) The real-time monitoring of the thermal unfolding of tetramethylrhodamine-labeled ... The measurement of actin concentration in solution: a comparison of methods Anal Biochem 62, 66–74 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage...

Ngày tải lên: 06/03/2014, 22:21

... represents an important step in assembly of the holoenzyme Results In vitro import and assembly of nDNA-encoded complex IV subunits The assembly of a number of nDNA-encoded complex IV subunits was ... assembly that involve formation of the catalytic core consisting of the mtDNA-encoded subunits CO1, CO2 and CO3 Thus details of the latter stages in which the majority of nDNA-encoded subunits that ... these differences is that further analysis of the biogenesis of the mammalian enzyme is required Assembly of nDNA-encoded complex IV subunits Of the five nDNA-encoded subunits investigated in this...

Ngày tải lên: 07/03/2014, 00:20

... representation of the relative densities of the free DNA measured at increasing concentrations of HbpR The percentage of free DNA was calculated from densitometric measurements of the radiolabeled ... resuspended in Mops medium [5.5 gÆL)1 of Mops free acid, 5.1 gÆL)1 of Mops sodium salt, 0.5 gÆL)1 of NaCl, gÆL)1 of NH4Cl, 0.06 gÆL)1 of Na2HPO4Æ2H2O, 0.05 gÆL)1 of KH2PO4, mm MgSO4, 0.1 mm CaCl2, ... 7, like mutant 5, was impaired in binding of the R13 region Both of these patterns contributed to the loss of binding contacts of mutant 72 Construction of a DA HbpR derivative In order to study...

Ngày tải lên: 07/03/2014, 17:20

... oxidation of fatty acids • the oxidation of pyruvate in the citric acid cycle Alternate DNA structures DNA exists in many possible conformations that include A -DNA, B -DNA, and Z -DNA forms only B -DNA ... Proof that DNA is carrier of genetic information - 1928 • Griffith‘s experiment - Bacterial transformation • Recombination of bacterial genetic material by transmission of naked DNA into ... interphase Nucleosome: DNA double helix + histone core • Histone core = octameres of two copies of H2A, H2B, H3, H4 • DNA double helix is winded around the core • spacer segment DNA between two nucleosomes...

Ngày tải lên: 13/03/2014, 16:57

... D melanogaster v-Jun of Homo sapiens dUTPase of Homo sapiens H2B of Saccharomyces cerevisiae c-Myc of Homo sapiens dUTPase of D melanogaster c-Myc of Xenopus laevis RanBP3 of Homo sapiens Bipartite ... physiological isoforms of the enzyme were identified, with apparent molecular masses of 69 and 63 kDa for the native homotrimers (termed long isoform, LD-DUT, and the N-terminally truncated short isoform, ... detection of cellular compartments Results and Discussion Subcellular targeting of UDE Nuclear targeting of UDE may be critical for performance of the suggested degradation function on genomic DNA...

Ngày tải lên: 15/03/2014, 11:20

... perturbation of the DNA- binding interface showed that, in the case of HPV-16, most of the binding energy comes from a ‘direct readout’ recognition mode [22] In fact, recognition of DNA by proteins, ... the presence of Lys349 in the loop connecting helix a2 and strand b4 This residue may have a role in DNA binding, as a single mutation of Lys349 to alanine weakens the DNA binding of the HPV-16 ... in the DNA recognition kinetics and in the stability of the complex [22], suggesting that there is an involvement of this loop in HPV-16 E2 DNA recognition Another interesting aspect of HPV-16...

Ngày tải lên: 16/03/2014, 10:20

... Binding of ATP to DnaK promoted quenching of the single Trp residue of DnaK and a blue-shift of its emission maximum (Fig 4B, upper traces) In addition to these spectral changes, reduction of the ... structure for DnaK and mtHsp70 [35], and also indicates that sequence exchange at the PBD of DnaK did not modify the overall secondary structure of the chimeras The intrinsic fluorescence of DnaK has ... should be noted that in the absence of nucleotide the emission maximum of mtHsp70 is downshifted by 5–6 nm with respect to that of DnaK The twofold reduction of the KSV values estimated for mtHsp70...

Ngày tải lên: 16/03/2014, 22:20

... failure of the primer 2 DNA template hybrid primer extension reaction was due to the effects of the hairpin structure on the process of primer annealing to the DNA template Analysis of secondary ... buffer (10·) containing mM of each dNTP, 10 mM ATP, 100 mM Tris/ Cl (pH 8.0), 50 mM MgCl2, 20 mM dithiothreitol, one unit of T4 DNA polymerase and three units of T4 DNA ligase, and incubating ... conditions of the DNA polymerase-catalysed primer extension reactions Under the same conditions, if the primer 1 DNA template hybrid appeared to be extended by DNA polymerase while the primer 2–DNA...

Ngày tải lên: 23/03/2014, 13:20

... EDITSEQUENCE programs (DNASTAR Inc.) Comparison of amino-acid sequences of M.MspI (P11408) with those of topoisomerases (Fig 1A) and DNA ligases (Fig 1B) revealed a region of similarity; better ... 399–458 of human topoisomerase I; P11387 [29]; Fig 1A), whereas the C-terminal portion of M.MspI (245–418) has sequence similarity to the active site of DNA ligases (region 543–584 of human DNA ligase ... sequence 32–98 of M.MspI was found to have similarity to a region of the topoisomerases, and the sequence 245–287 had similarity to members of the DNA ligase family The N-terminal portion of M.MspI...

Ngày tải lên: 24/03/2014, 00:21