querying the database using ado net
Báo cáo y học: " Upregulated Genes In Sporadic, Idiopathic Pulmonary Arterial Hypertension" pdf
Ngày tải lên :
12/08/2014, 16:20
... fingers of the Cys4, CysHis2Cys type that is not present in the other two prolyl hydroxylases The function of the MYND zinc finger domain of EGLN1 is not known, but the MYND domain of the myloid ... monooxygenase (FMO2) is the major FMO enzyme in the lungs of many species, being localised to the pulmonary epithelium and plays a role in the in the oxidation of many xenobiotics and therapeutic drugs ... identified and further studies using techniques such as in situ hybridization are required to elucidate their localization in the lung These genes are candidates for further study in the pathology...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
Ngày tải lên :
18/03/2014, 01:20
... than 50% of the native enzyme (Fig 1C) The distribution of the KE2 mutant between the two fractions was similar to that of the native enzyme (Fig 1C) In contrast, the activity of the KE2RS2 mutant ... and KM20L2 cells (Fig 4B) The expression of other sPLA2s (IB, IIE and IIF) was undetectable The expression of other enzymes involved in the PGE2biosynthetic pathway in these colon carcinoma cell ... tissue-specific Searching the nucleic acid database reveals the presence of the TATA box and the binding motifs for AP-1 and NFjB in the putative 5¢-flanking promoter region of the human sPLA2-IID gene,...
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CURRENT BASIC AND PATHOLOGICAL APPROACHES TO THE FUNCTION OF MUSCLE CELLS AND TISSUES – FROM MOLECULES TO HUMANS pdf
Ngày tải lên :
28/06/2014, 15:20
... representing the position of the particle, were also taken to represent the position of the myosin head The position of the myosin head, determined by the above method, was compared between the two ... by the “slow” calcium pump SERCA2b (the only isoform of SERCA expressed in synthetic VSMCs) The depleted store triggers the translocation of STIM1 towards the plasma membrane, which, through the ... the movement of the myosin heads still remains as a matter of debate and speculation The reason for the present situation in the field of muscle research arises from the fact that the myosin head...
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Biopreservation of cells and engineered tissues
Ngày tải lên :
22/10/2014, 17:43
... [92–94] The hypothermiainduced inhibition of transmembrane pumps, such as the Na+ /K+ ATPase and the mitochondrial electron transport system disrupts the ability of the cell to maintain the necessary ... in the concentration of solutes in the unfrozen fraction The development of a chemical potential difference across the cell membrane provides the driving force for the efflux of water from the ... then the scale of damage done to the cells can be significantly reduced Minimizing the detrimental effects of ice formation during freezing and thawing has therefore been the focal point for the...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... culture, the medium was refreshed, and then the medium changed every other day Cell passaging was performed when cell confluence reached 85% The purity and immunophenotype of MSCs and their potentials ... were placed on the platform, the background image was taken under the lights of an illuminator http://www.medsci.org Int J Med Sci 2011, Then, the illuminator was turned off, and the image of light ... feasible and safe Another concern of intravenous transplantation of MSCs is whether the MSCs can migrate into and proliferate in the target tissues, which is the basis of therapeutic effects of...
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HUMAN MILK OLIGOSACCHARIDES: CHEMICAL STRUCTURE, FUNCTIONS AND ENZYMATIC SYNTHESIS
Ngày tải lên :
28/08/2013, 16:28
... gestation, and with genetic makeup of the mother (McVeagh and Miller, 1997) Amount of HMOs is the highest in the newborn period, rising during the first days and then reducing after the first months ... identification of the HMOs is still lacking because of the complexity and the diversity of the structures (Rockova et al., 2011) Basically, most HMOs contain a lactose at the reducing end as the core ... while Lactobacillus gasseri, another gut commensal did not ferment HMO These results support the hypothesis that HMOs selectively affect the commensal bacteria in the intestinal tract 2.3.2 Potential...
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Tài liệu Báo cáo khoa học: a-Defensins increase lung fibroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc
Ngày tải lên :
18/02/2014, 06:20
... activate the b-catenin signaling pathway We then studied the role of the b-catenin signaling pathway in a-defensininduced increases in the proliferation and collagen synthesis of lung fibroblasts using ... collagen synthesis of lung fibroblasts The stimulatory effects of a-defensins on collagen synthesis reach a plateau at higher concentrations, whereas the proliferative effects are reduced The results ... In the present study, we also found that a-defensin-1 and a-defensin-2 stimulated the proliferation and collagen synthesis of two other cell lines of lung fibroblasts (HFL-1 and LL86) However, the...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
Ngày tải lên :
20/02/2014, 23:20
... cellulase Then, a forward primer F1 was synthesized on the basis of the N-terminal sequence of the HdEG66, while reverse primers R1 and R2 were synthesized on the basis of sequences of LP5 and LP6, ... indicating that the thus cloned cDNAs are of the HdEG66 protein It is noteworthy that the N-terminal 15 residues in the deduced sequence are absent in the HdEG66 protein According to the sequence ... solid lines Bold lines in both sides of the cDNAs indicate primers used for the PCR Lengths of the cDNAs are shown in the parentheses (B) Structure of the genomic fragment, Hdcel-1 DNA Open and...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Ngày tải lên :
21/02/2014, 15:20
... locks the enzyme in the active form for CTP synthesis and thereby stimulates the kcat of the reaction Since GTP actually will activate the uncoupled glutaminase reaction the structure of the monomer ... similar to that of the overall CTP synthesis reaction It was concluded that the effect of GTP was mainly to enhance the rate of chemical steps of the glutaminase reaction The nding that the kcat value ... directly obtained from the data les as the value recorded just prior to the subsequent injections Dividing the power with the molar reaction enthalpy gives the steady-state rate The assay conditions...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
Ngày tải lên :
05/03/2014, 17:20
... coincident with the bottom surface of the ultrasound chamber with the center of rotation offset mm from the center of the transducer face The movement of the transducer mimics its use as a therapeutic ... of the field output over time The distance between the transducer and the scrotum was initially set to cm In an attempt to increase the energy delivered to the testes, the distance between the ... effect on the scrotal epithelia or the dartos fascia (muscular tissue under the skin) that enhances the transmission of ultrasound energy The dartos fascia is responsible for the furrowing of the scrotal...
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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
Ngày tải lên :
07/03/2014, 11:20
... at the ceiling of the cavity The dose absorbed by the irradiated object is constantly measured by a radiometer present in the apparatus, the measurement depending on the ageing of the lamp The ... In the second phase, a series of experiments using NHDFc was performed to confirm the effect of RFR exposure on Hsc70 On the one hand, the Hsc70 expression pattern was different and, on the other ... placed at the centre of the cross-linker at a GSM-900 and cell stress in skin models distance of 13.5 cm (minus the height of the culture dish) from the UV lamp The apparatus calculates the duration...
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Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
Ngày tải lên :
07/03/2014, 21:20
... reaching a peak at the wandering stage late in the last larval stadium At the prepupal stage, EH activity declined to a level equal to that in the early time of the stadium Northern blot analysis ... out using the first round PCR product as a template From the nucleotide sequence of the second PCR product, an additional primer corresponding the internal partial nucleotide sequence was synthesized ... partial sequence of the DmEH The full-length DmEH ORF was amplified by PCR from first strand cDNAs using Pfu polymerase (Promega) The forward primer corresponded to the 5¢-end of the ORF and contained...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
Ngày tải lên :
08/03/2014, 22:20
... expression vector of the pSV2 plasmid series [19] The HCII cDNA spanned the protein coding sequence (including the signal peptide) down to the EcoRI site at position 1559 in the 3¢ untranslated ... irradiance at the surface of 0.2 mm2 spots In addition to the positive mode standard procedure for the detection of sulfated peptides using the reflectron for enhanced resolution, the peptide mixture ... transmitted from the quadrupol mass analyzer into the collision cell Argon was used as the collision gas and the kinetic energy was set from )15 to )60 eV for optimal fragmentation The resulting...
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Lecture 3 DNA RNA and protein synthesis great
Ngày tải lên :
13/03/2014, 16:36
... sequence codes The messenger RNA leaves the nucleus and attaches to a ribosome in the cytoplasm Transfer RNA brings amino acids to the ribosome to match the sequence codes At the ribosome, Amino ... molecules found in the food we eat or produced in our cells It happens at the ribosomes DNA and protein synthesis, then, happens this way: DNA sequence codes (how letters are put together) produce ... “missing oxygen” (that’s the de-oxy part) And it’s found in the nucleus of eukaryotic organisms How does DNA code for protein synthesis? First-what is “protein synthesis”? It’s building,...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
Ngày tải lên :
15/03/2014, 09:20
... or the brain, in the adult body They can renew themselves in the body, making identical copies of themselves for the lifetime of the organism, or become specialized to yield the cell types of the ... secure the services of eminent members of appropriate professions in the examination of policy matters pertaining to the health of the public The Institute acts under the responsibility given to the ... by the Academy, the Council has become the principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the...
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Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc
Ngày tải lên :
16/03/2014, 04:20
... treated using laser scanning confocal microscopy with serial confocal sections (xy, 0.2 lm) collected from the top to the bottom of the cell monolayer Then, xz sections were displayed using the ortho ... internalize the antibody for 30 at 37 °C, and the temperature was then shifted again to °C The cell surface was then stripped of any remaining antibody with an acid wash (0 min) The cells were then ... monitored using a specific antibody to GD3 and CTx, respectively Therefore, the results described in this work provide the basis to gain further insight into the molecular mechanisms that operate in the...
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Định lượng mô, tế bào bằng phân tích hình ảnh (Quantifying tissues, cells by image anaslysis) potx
Ngày tải lên :
20/03/2014, 01:20
... máy vi tính theo chơng trình phần mềm xử lý hình ảnh để tăng cờng chất lợng ảnh (tăng độ tơng phản, xác định ranh giới, tăng giảm mầu sắc); tín hiệu số máy tính đợc tự động điều chỉnh theo Có cách ... tìm quét thiết bị đổi tín hiệu điện tơng đồng thành tín hiệu số Thiết bị số hoá đợc chế tạo tuỳ theo chức có lực làm việc riêng tuỳ loại Với ảnh siêu cấu trúc (hình ảnh đen trắng), việc thu nhận ... biên độ (sửa đổi dãy số giá trị xám) * Chuyển đổi hình ảnh không gian * Chuyển đổi hình ảnh mầu theo nguyên lý phân tích Fourier * Chuyển đổi hình ảnh phơng pháp mầu giả Lu ý cách tổng quát để...
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simple and rapid synthesis of a-fe2o3 nanowires under ambient conditions
Ngày tải lên :
20/03/2014, 13:07
... controlled by observing the color of the wire and by varying the applied heating power (see the ESM) SEM observation of the wire after the synthesis of the reddish material revealed that the wire was completely ... to the top of the growing NW is surface diffusion The sword-like shape of the NWs confirms that the growth is determined by a diffusion process from the bottom—where the NWs are thicker—to the ... magnitude lower and thereby cannot significantly affect the ion motion across the wire The iron oxidation process involves iron ion diffusion from the iron wire core to the surface through the iron oxide...
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Báo cáo khoa học: LRRK2 in Parkinson’s disease: function in cells and neurodegeneration doc
Ngày tải lên :
23/03/2014, 04:20
... Elucidating the normal function of LRRK2, and the disease-inducing functions mediated by mutant LRRK2, promises the opportunity to unveil the molecular basis of PD, as well as the discovery of novel therapeutic ... activity), it is relatively clear that the kinase activity of b-RAF is the oncogenic activity associated with the protein [6] Other pathogenic LRRK2 mutations localize to the Ras of complex proteins (ROC) ... retraction, whereas another one in ten show involvement in neurite extension [35] In the described screen, the MAPKKK proteins and other tyrosine-kinase-like family members heavily populate the pool of...
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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
Ngày tải lên :
29/03/2014, 08:20
... mitochondrial ATP biosynthesis [47] Taken together, these observations suggest cell type differences in PHB action that need to be confirmed in further studies The present study did not address the mechanism ... and methods The results are expressed as the mean ± SEM percentage of G2 for (A) and (B); as the mean ± SEM arbitrary units relative to actin for (C); and as the mean ± SEM for (D) the low (5.5 ... prompted us to investigate whether PHB affected mitochondrial function in these cells Because PHB is found in the circulation, we hypothesized that it may enter the cells His-tagged recombinant...
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