quantitative pcr and real time quantitative pcr
báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
Ngày tải lên :
12/08/2014, 03:20
... both rice and Arabidopsis thaliana, and are distributed non-randomly throughout the genome [30-32] Clustering of R genes is known to facilitate tandem duplication of paralogous sequences and generation ... closely-related cereal species (wheat, oat and barley), rice and Arabidopsis Primer design for candidate Lolium R genes Locus amplification primers (LAPs) for multiple target genes were designed using standard ... cereal species including barley, wheat, rye and oat [33,59] Results Strategies for specific R gene isolation Three strategies (empirical approaches based on heterologous PCR and degenerate PCR, ...
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Báo cáo y học: "Validation and refinement of gene-regulatory pathways on a network of physical interactions" pps
Ngày tải lên :
14/08/2014, 14:21
... Vertical bars indicate the standard deviations for the random selection method The standard deviations for the information and hub selection curves are less than five and are not shown for clarity ... NIGMS grant GM070743-01 and NSF grant CCF-0425926 T.I was supported by a David and Lucille Packard Fellowship award C.Y and T.J were supported in part by NIH grant(s) GM68762 and GM69676 10 11 12 ... combination of previously documented and novel findings First, in agreement with previous literature, we confirm that MSN4 and HAP4 are inducers [27,28] and that SOK2 is a repressor [29] For...
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Báo cáo y học: "he discovery, positioning and verification of a set of transcription-associated motifs in vertebrates" pps
Ngày tải lên :
14/08/2014, 15:20
... both real and random promoterwise alignments select this threshold We performed simulation studies with random sequence that showed that bit scores >20 bits are extremely rare when aligning randomly ... evolutionary aspects see Wray et al [10] and for a review from the bioinformatics perspective see Wasserman and Sandelin [11] A motif is a subsequence of DNA of between and 20 base pairs (bp) of fixed ... space and time efficient manner, we developed a simpler approach along the lines of the same greedy approach (see Materials and methods) lists these 55 motifs (some occur in both the CpG and the...
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báo cáo hóa học:" Cross-cultural validation and analysis of responsiveness of the QUALIOST®: QUAlity of Life questionnaire In OSTeoporosis" pptx
Ngày tải lên :
20/06/2014, 15:20
... SOTI study (semi -quantitative and quantitative) and as a major clinical expert in the SOTI study, also validated the clinical aspects of the article PJM is the SOTI coordinator and as a major clinical ... (M0) and then every six months using the SF-36 and QUALIOST® The 12 countries of the SOTI trial were Australia, Belgium, Denmark, France, Germany, Greece, Hungary, Italy, Poland, Spain, Switzerland ... consent and the protocol was approved by the Ethics Committee Baseline and annual X-rays were provided and assessed by a centralised procedure (Pr C Roux, Cochin Hospital, France) The prevalent and...
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HIV INFECTION IN THE ERA OF HIGHLY ACTIVE ANTIRETROVIRAL TREATMENT AND SOME OF ITS ASSOCIATED COMPLICATIONS docx
Ngày tải lên :
27/06/2014, 18:20
... Neuropathies Katrin Hahn and Ingo Husstedt Chapter Spondylodiscitis and HIV – Diagnosis and Treatment Strategies 121 Jan Siewe, Kourosh Zarghooni and Rolf Sobottke Chapter Acute Abdomen and HIV Infection ... diabetes, and use of lipid lowering and hypertension therapy 20,775 HIV-infected and 215,158 HIV negative members contributed to 90,961 and 1,133,333 persons-years (py) respectively HIV-infected and ... Atazanavir/ritonavir ↑ LDL-c TG and no change HDL-c Darunavir/ritonavir ↑ TC, LDL-c, TG and no change HDL-c Fosamprenavir/ritonavir ↑ TC, LDL-c, TG and no change HDL-c Indinavir ↑ TC, LDL-c, TG and Lopinavir/ritonavir...
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Báo cáo y học: "Perspectives and limitations of gene expression profiling in rheumatology: new molecular strategies" doc
Ngày tải lên :
09/08/2014, 01:23
... and will reduce our screening and analysis efforts to the principal components and actors Strategies Suggestions by Firestein and Pisetsky [41] underline the importance of an understandable and ... retroviral transcripts in renal tubules and also in brain and lung tissue Azuma and colleagues used microarrays for the detection of new candidates in salivary gland tissue from the MLR/MpJ-lpr/lpr ... and less expense, with the use of real- time PCR technology prefabricated on a card system with up to 384 different reactions In addition, with a relatively low investment, with less working time...
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Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays" pptx
Ngày tải lên :
09/08/2014, 20:20
... (left) and the robustness of predictions in the face of increasing noise levels (right) these unsuccessful candidates early and at low cost Conversely, careful selection and optimization of candidate ... and candidate biomarkers (Additional data file 1); the XML-based PMML model that MethMarker uses for exporting, importing and storing candidate biomarkers (Additional data file 2) importing and ... immunoprecipitation quantitative PCR (MeDIPqPCR)) Third, the accuracy of all designed assays is computationally assessed, using the DNA methylation map derived in the first step Fourth, the most promising candidate...
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báo cáo khoa học: " Identification, utilisation and mapping of novel transcriptome-based markers from blackcurrant (Ribes nigrum)" pdf
Ngày tải lên :
11/08/2014, 11:21
... from developing fruit and buds, and Sanger-sequenced several thousand ESTs [13,14] From these libraries, forty-three SSR and sixteen SNP markers have been mapped genetically and, together with AFLPs, ... design and molecular biology, and facilitated the 2GS procedures MB and LC provided bioinformatics support for the 2GS data CH analysed the mapping data CB and JAM provided sequencing and genotyping ... genetic and environmental factors to trends and variability in yield over time Theor Appl Genet 2011, 122:225-238 Wall PK, Leebens-Mack J, Chanderbali AS, Barakat A, Wolcott E, Liang H, Landherr...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
Ngày tải lên :
12/08/2014, 03:20
... CH3CN, 0.1% HCOOH and 80% CH3CN, 19.9% H2O, 0.1% CH3COOH The percentage of the latter reached the 60% over a 15 run time, and 100% after 28 Real- time PCR experiments For real- time PCR assays, UV-C ... http://www.biomedcentral.com/1471-2229/9/30 Real- time PCR In order to assess the involvement in the response to UVC irradiation, the expression levels of HQT and HCT were analysed by real- time PCR Based on normalized ... Laboratories, USA), specific primers at 300 nM, and μl of cDNA PCR reactions were carried out in 48-well optical plates using the iCycler Real- time PCR Detection System (Bio-Rad Laboratories, USA)...
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Báo cáo y học: "Value and price of ventilator-associated pneumonia surveillance as a quality indicator" potx
Ngày tải lên :
13/08/2014, 20:21
... interdisciplinary team Crit Care Med 2006, 34:211-218 Horan TC, Andrus M, Dudeck MA: CDC/NHSN surveillance definition of health care-associated infection and criteria for specific types of infections in the ... Intensive Care Med 2004, 30:996-997 doi:10.1186/cc8189 Cite this article as: Aardema LM, et al.: Value and price of ventilator-associated pneumonia surveillance as a quality indicator Critical Care 2010,...
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Báo cáo y học: "Strategy for encoding and comparison of gene expression signatures" pps
Ngày tải lên :
14/08/2014, 07:22
... hit ratio, and this is used to rank the confidence levels of data set matches reports Where N and M are numbers of concordant and discordant matches, respectively, and Siq and Sis (Sjq and Sjs ) ... include those reported by Staunton and coworkers [13] (dataset A), Butte and colleagues [12] (dataset B), Ross and coworkers [14] (dataset C), and GDS89 Datasets A and B were generated using Affymetrix ... described in the Materials and methods section (below) Design and validation of EXALT The EXALT system consists of four components (two program pipelines, SigDB, and search engine) and a web interface...
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Báo cáo y học: "Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish" pdf
Ngày tải lên :
14/08/2014, 08:20
... STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Synteny between regions containing STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Genes ... al R251.21 CS and ML conducted BLAST searches, made alignments and phylogenetic trees, made the figures and wrote the text MC identified the cytokine and cytokine receptor genes and analyzed their ... R251.3 Figure Components of the TLR and IFN signaling pathways and intracellular pattern recognition receptors Components of the TLR and IFN signaling pathways and intracellular pattern recognition...
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Báo cáo y học: " Validation and extension of an empirical Bayes method for SNP calling on Affymetrix microarrays" pot
Ngày tải lên :
14/08/2014, 08:21
... (Nsp and Sty) from the Broad Institute and Affymetrix, respectively Again, the gold standard is HapMap calls (c) ADPs are plotted for the 32 and 16 high quality replicates hybridized on XBA and ... HapMap and Affymetrix and, thus, these SNPs were corrected by hand For the replicate data, high quality chips (operationally defined as having an SNR greater than 4.5 and 2.36 for the 100K and 500K ... Hubbell and Chuck Sugnet for helpful discussions; and finally, Ben Bolstad, James MacDonald, Seth Falcon, Marvin Newhouse, Robert Gentleman, Jiong Yang, and Fernando Pineda for help with hardware and...
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Báo cáo y học: " Advances in the genetics and epigenetics of gene regulation and human disease" pps
Ngày tải lên :
14/08/2014, 17:22
... recombination and the position of cross-overs Using in silico and in vitro analyses, Sapienza’s group have shown a relationship between increased rates of meiotic recombination and genomic imprinting ... several generations of offspring He and his colleagues postulate that maternal nutrition before and during pregnancy may affect the establishment of CpG methylation and the life-long expression of ... CpG island methylation rate with 90% accuracy, which was later confirmed by in vitro analyses This study revealed that the DNA composition of CpG islands, the sequence, the structure and the...
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Accelerating Test, Validation and Debug of High Speed Serial Interfaces
Ngày tải lên :
09/04/2015, 17:22
... where standard instruments, such as oscilloscopes, signal generators and logic analyzers are used The standard measurement equipment is also referred to as bench equipment, and the validation and ... throughput and has been widely used in production test It is also more and more widely use for validation and characterization to shorten the time- to-market To validate, characterize and test HSSIs ... the time deviation and amplitude deviation can cause a bit error – bit “0” is received as bit “1” or bit “1” is received as bit “0” 2.2 Timing Jitter 21 If we ignore the setup time and hold time...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
Ngày tải lên :
16/02/2014, 15:20
... interacting candidates and were treated with arsenite (1 mM for 30 min) Cells were fixed and stained with mouse anti-HA and goat anti-TIAR sera Secondary Alexa 594-coupled donkey anti-mouse (red) and FITC-coupled ... ASF/SF2 is associated with TIAR and TIA-1 protein complexes and is a bona fide SG component Because ASF ⁄ SF2 and TIAR shared similar localization patterns both in normal and stressed cells, we FEBS ... candidates Cells were lysed and flag-tagged proteins were immunoprecipitated with sepharose beads coupled with M2 anti-flag serum Inputs and immunoprecipitates (IP) were analyzed by SDS-PAGE and...
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Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx
Ngày tải lên :
31/03/2014, 07:20
... seven times higher in brain than in testes and between 50 and 160 times higher in brain than in muscle, intestine and heart No product was generated by qRT -PCR from liver, ovary, skin and kidney ... between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is higher in the exons and than it ... DNA and gel-purified B-FABP and b-actin RT -PCR products were allowed to bind SYBRÒ Green dye and the amount of bound SYBRÒ Green I was determined by fluorimetry The concentration of B-FABP and...
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Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot
Ngày tải lên :
31/03/2014, 07:20
... capped and complete zebrafish fabp3 gene transcripts A major band of approximately 200 bp, and two minor bands of 250 bp and 180 bp, were observed after agarose gel electrophoresis of nested PCR ... double underlined and an alternative polyadenylation site is marked with a star Underlined sequences correspond to primers used in PCR and RT -PCR experiments (see Materials and methods) A single ... gel-electrophoresis Three bands of 250 bp, 200 bp and 180 bp in the TAP+ reaction were excised from the gel and purified using the Qiaquick gel extraction kit (Qiagen) The two minor bands ( 250 bp and 180...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
Ngày tải lên :
20/06/2014, 01:20
... previously described [63] RNA isolation and quantitative real- time PCR Total RNA was isolated from uninfected, uninfected Vero cell lysate treated, and RSV and RSV mutant virus infected (MOI = 1) ... of a classical negative feedback loop that is time- dependent [24], thus RSV and RSV mutant virus infection of MLE-15 cells and IFNα, IFNβ and SOCS1 and SOCS3 mRNA expression was examined at 48 ... expression also increased The levels of IFNα and IFNβ and SOCS1 and SOCS3 mRNA expression were similar between WT and ΔG virus infected MLE-15 cells Comparing time- points post-WT or ΔG virus infection,...
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