purified hematopoietic stem cells can differentiate into hepatocytes in vivo

... These data suggest Stem- Kine supplementation increases circula- tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells with EPC phenotype Agents ... administra- tion of mobilizing agents. In this manner the increased number of circulating stem cells are more available to respond to injury signals, hypothetically resulting in enhanced healing. Granulocyte ... chemotactic interaction during hematopoietic stem cell mobilization induced by G-CSF or cyclophosphamide. J Clin Inves 2003, 111:187-196. 69. Cavaillon JMH-CN: Signals involved in interleukin 1 synthesis...

Ngày tải lên: 18/06/2014, 16:20

... proteins from HSCs/CD34 + cells in HbE/b- thalassemia. We categorized candidate proteins into two major groups. First is the common apoptotic proteins associated with the extrinsic and intrinsic ... caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act ... phosphoproteins in HbE/b-thalassemic stem cells was proposed (Figure 4). Discussion Conventional methods typically have difficulty using fresh human hematopoietic stem cells for studying cell signaling in...

Ngày tải lên: 18/06/2014, 19:20

... phosphoproteins in HbE/b-thalassemic stem cells was proposed (Figure 4). Discussion Conventional methods typically have difficulty using fresh human hematopoietic stem cells for studying cell signaling in ... caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act ... mechanism responsible for induction of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood. Hematopoietic stem cells (HSCs) contain specific markers...

Ngày tải lên: 20/06/2014, 04:20

... research in hematopoiesis that includes defining stepwise molecular changes accompanying functional changes in maturing cells, aggregating cells into functional subgroups, and demonstrating hematopoietic ... Chapter 068. Hematopoietic Stem Cells (Part 1) Harrison's Internal Medicine > Chapter 68. Hematopoietic Stem Cells Hematopoietic Stem Cells: Introduction All of the cell types in the ... know about stem cells is imprecise and based on inferences from genetically manipulated animals. Cardinal Functions of Hematopoietic Stem Cells All stem cell types have two cardinal functions:...

Ngày tải lên: 07/07/2014, 01:20

... Chapter 068. Hematopoietic Stem Cells (Part 2) Developmental Biology of Hematopoietic Stem Cells During development, blood cells are produced at different sites. Initially, the yolk ... progressively increasing in the complexity of cell types from those simply carrying oxygen to platelets supporting a more complex vasculature to the cells of innate immunity and finally to the cells ... the CXCR4/CXCL12 interaction, cleavage of CXCL12, or downregulation of the receptor can all result in the release of stem cells into the circulation. This process is an increasingly important...

Ngày tải lên: 07/07/2014, 01:20

... Chapter 068. Hematopoietic Stem Cells (Part 3) Excess Capacity of Hematopoietic Stem Cells In the absence of disease, one never runs out of hematopoietic stem cells. Indeed, serial transplantation ... proliferation. Hematopoietic stem cells have governing mechanisms that are distinct from the cells they generate. Hematopoietic Stem Cell Differentiation Hematopoietic stem cells sit at ... colony-stimulating factor drives the rapid proliferation of granulocyte precursors but does not affect cell cycling of stem cells. Rather, it changes the location of stem cells by indirect means, altering...

Ngày tải lên: 07/07/2014, 01:20

... restrict entry of stem cells into cell cycle, blocking the G1-S transition. Modifying the levels of molecules such as p21Cip1 and p18INK4c in the laboratory has resulted in increased stem cell proliferation ... angiopoietin 1 on osteoblasts. The regulation of stem cell proliferation also appears to change with age. In mice, the cyclin-dependent kinase inhibitor p16INK4a accumulates in stem cells in older ... quiescent. In the hematopoietic system, stem cells are also highly cytokine-resistant, remaining dormant even when cytokines drive bone marrow progenitors to proliferation rates measured in hours,...

Ngày tải lên: 07/07/2014, 01:20

... refractory to interventions aimed at dividing cells. Does the concept of cancer stem cells provide insight into the cellular origin of cancer? The fact that some cells within a cancer have stem cell–like ... experimentally in the hematopoietic system. Taking advantage of the cell-surface markers that distinguish hematopoietic cells of varying maturity, stem cells, progenitors, precursors, and mature cells can ... determining body patterning in invertebrates. HoxB4 is capable of inducing extensive self-renewal of stem cells through its DNA-binding motif. Other members of the...

Ngày tải lên: 07/07/2014, 01:20

... 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures 4 and 5b). Aggrecan matrix staining within the vicinity of the injected cells ... or Alizarin red staining was observed at any time point. Conclusions MSCs from older individuals differentiate spontaneously into chondrocyte-like NP cells upon insertion into NP tissue in vitro, ... forma- tion or mineralisation in the IVD tissue 4 weeks post injection. The finding that such cells not only survive following injection into IVD tissue but appear to redifferentiate into a chondro- cyte-like...

Ngày tải lên: 09/08/2014, 01:22

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Ngày tải lên: 11/08/2014, 07:21

Ngày tải lên: 12/08/2014, 18:21

... Access Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK Hsiao-Yun Lin 1,2† , Chih-Chien Tsai 1,4† , Ling-Lan ... differentiation with increases in insulin and Glut2 gene expressions, proinsulin and insulin protein levels, and insulin release in response to elevated glucose concentration. Adding FN or LAM induced activation ... mar- row stem cells or MSCs to differentiate into IPCs has been demonstrated in vitro and in vivo. Bone marrow cells when transplanted into lethally irradiated recipient mice expressed insulin in the...

Ngày tải lên: 10/08/2014, 05:21

... like intercalated disc. Our results Figure 2 Immunostaining of anti-a-actinin and anti-a MHC in cells at different time points of culturing. A strong expression of both a-actinin and MHC proteins ... demonstrated in Figure 2, Immunostaining with specific antibodies revealed that cardiomyocyte markers including myosin heavy ch ain (MHC) and sarcomeri c a- actinin were strongly expressed in differentiated ... fluorescent immunostaining of a- actinin of cells from three groups, while, J-L shows the fluorescent immunostaining of MHC of cells from these groups after 5 and 10 days’ culturing. Cells from CMCM and...

Ngày tải lên: 10/08/2014, 05:21

... convert the 32D cells to cytokine independence. In agreement with pre- vious reports [21,22], 32D cells proliferate as undifferen- tiated blasts when maintained in IL-3, but differentiate into mature ... for expanding clinically transplantable stem cells. Keywords: Mouse Hematopoietic Stem Cell, Transplantation, Differentiation Background Hematopoietic stem cells (HSCs) are rare cells def ined by ... Mitogen-activated protein kinase inhibition induces translocation of Bmf to promote apoptosis in melanoma. Cancer Res 2009, 69:1985-94. 36. Yang J, Chai L, Liu F, Fink LM, Lin P, Silberstein LE, Amin HM, Ward...

Ngày tải lên: 10/08/2014, 21:23

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Ngày tải lên: 11/08/2014, 12:21

Ngày tải lên: 11/08/2014, 12:21

Ngày tải lên: 14/08/2014, 08:20