purification of pcr product dna fragments from agarose gel
Genetic approaches to study liver development in zebrafish core component sec13 in COPII complex is essential for liver development in zebrafish
Ngày tải lên :
14/09/2015, 12:11
... 42 2.3.1 Polymerase chain reaction (PCR) 42 2.3.2 Purification of PCR product/ DNA fragments from agarose gel 43 2.3.3 Ligation of DNA insert into plasmid vectors 43 2.3.3.1 ... 44 2.3.4.3 Isolation of plasmid DNA from E coli 44 2.4 DNA sequencing 44 2.5 Zebrafish genomic DNA preparation 45 2.5.1 Prepare genomic DNA from adult zebrafish ... x List of Table Table 2-1 Typical reaction composition of PCR 42 Table 2-2 Typical thermal cycler conditions of PCR 42 Table 2-3 Typical reaction composition of sequencing PCR ...
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Thiết lập quy trình điện di protein SDS -PAGE và ứng dụng đánh giá phản ứng của cây lúa
Ngày tải lên :
06/11/2012, 09:49
... thƣờng sử dụng phƣơng pháp điện di gel không liên tục (discontinuous gel) Trong phƣơng pháp gel gồm lớp: Lớp gel gom (stacking gel) : Thông thƣờng lớp gel có nồng độ gel thấp vào khoảng – 5%, nằm phía ... bơm từ từ dung dịch gel vào khuôn, đổ gel cho mức dung dịch gel cao cm (tránh tạo bọt khí khuôn gel) Sau đó, nhẹ nhàng đặt lớp nƣớc cất lên lớp gel để mặt gel đƣợc phẳng Chờ gel đông (khoảng 20 ... dịch nạp mẫu cách đáy gel khoảng 0,5 – 1cm Sau điện di, tháo gel khỏi khuôn, rửa gel nƣớc phút (cẩn thận tránh làm đứt gel) Nhuộm gel thời gian 30 – 45 phút Sau nhuộm xong, rửa gel với nƣớc phút...
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MÔ HìNH HOá QUá TRìNH TRAO ĐổI NHIệT ẩM TRONG MáY ấP TRứNG GIA CầM Modeling of heat and mass transfer in chicken egg incubators
Ngày tải lên :
28/08/2013, 08:31
... D McDaniel (2004), A Practical Manual for Understanding the Shell Structure of Broiler Hatching Eggs and Measurement of their Quality, Mississipi State University, p 3-5 ... Trans ASEA, pp 31 Husain A., Chen C.S., Clayton J T, Whitney L.F (1972), Mathematical Simulation of Mass and Heat Transfer in High Moisture Foods, Trans ASAE, pp 55-59 224 Husain A., Chen C.S., ... hợp hệ thống điều khiển cho toàn hệ thống Tài liệu tham khảo Crank J., (1975), The Mathematics of Diffusion, Oxford University Press, Bala, B K (1998), Solar drying systems: Simulations and optimization,...
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Effect of periodic suction on three dimensional flow and heat transfer past a vertical porous plate embedded in a porous medium
Ngày tải lên :
05/09/2013, 14:58
... number of research papers of some International Journals Dr Das is currently acting as the honorary member of editorial board of Indian Journal of Science and Technology and as Referee of AMSE ... (India) from 1982-2004 and presently working as the Head of the faculty of Physics in KBDAV College, Nirakarpur, Orissa (India) since 2004 He has 27 years of teaching experience and 10 years of research ... present the effect of suction parameter α and the Reynolds number Re respectively on the temperature of the flow field A careful observation of the above figures shows that the effect of growing suction...
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Arsenic and membrane toxicity
Ngày tải lên :
17/12/2013, 12:23
... Nuran Ercal et al./Current Topics in Medicinal Chemistry, (2001) 529-539 Fenton-like reactions of metals Sản sinh ROS (Reactive Oxygen Species) ROS Sự chuyển hóa sinh học arsenic vô động vật ... arsonoliposomes Time (h) Sự tương tác GSH với arsonoliposome gây nên hóa lỏng màng nhân tạo Journal of Nanoscience and Nanotechnology (2006) 2974–2978 Mạng lưới tín hiệu Các tín hiệu tế bào sinh dẫn ... 403–448 Các cấu trúc hóa học chất mang hòa tan màng …Arsenic liên kết với tất Ivan Stanish, Journal of Membrane Science 192 (2001) 99–113 Arsenoliposomes tác động mạnh lên tế bào ung thư, gây ảnh...
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Tài liệu UNIVERSITY ENTREPRENEURSHIP AND TECHNOLOGY TRANSFER: PROCESS, DESIGN, AND INTELLECTUAL PROPERTY doc
Ngày tải lên :
19/02/2014, 05:20
... Eller Center, The University of Arizona Of ce of Technology Transfer, The University of Arizona NBER, The University of Arizona Academy of Entrepreneurship, University of Illinois, Urbana-Champagne ... SIEGEL AND PHILLIP H PHAN scientist and industry scientists Research productivity is measured using three proxies: number of patents granted, number of products in development, and number of products ... the research productivity of Japanese firms, as measured by the rate of firm patenting, product innovation, and market introductions of new products However, they also report an absence of geographically...
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Tài liệu Taxation and Technology Transfer: Key Issues doc
Ngày tải lên :
19/02/2014, 08:20
... sources of this concrete and practical problem, linking solutions to more deep-going reforms The burdens were the product not simply of predatory or corrupt local of cials They were the product of ... resources, which were extracted from villagers in the form of miscellaneous taxes and assorted fees The process of extraction often turned predatory because of a lack of standardized, legally enforceable ... extraction of resources from Chinese peasants led to loss of support if not loss of legitimacy and therefore required higher levels of coercion to obtain compliance Inevitably, there is a trade-off,...
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Tài liệu Taxation and Technology Transfer: Key Issues ppt
Ngày tải lên :
19/02/2014, 08:20
... per cent of profits from the export of computer software or the provision of technical services related to software Japan allows a special deduction of the income derived from the export of certain ... per cent of the income derived from the transfer or licensing of technology Sri Lanka provides an exemption for income earned from the export of technology by means of the provision of professional ... export of technology For example: India permits the deduction (from taxable income) of 50 per cent of royalty and service fee income earned abroad from the use of patents or inventions, and of 100...
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
Ngày tải lên :
19/02/2014, 16:20
... recorded from 260 to 190 nm at a scanning rate of 50 nmÆmin)1 with a time constant of s, step resolution of 0.1 nm, and bandwidth of nm The final spectra were taken from the average of five scans ... attributed to bH of Ala12 and NH of Val15, and aH of Pro13 and NH of Val15 Ó FEBS 2004 Structural study of internal fusion peptide of ASLV (Eur J Biochem 271) 4733 Conformational alteration of IFP–wt ... of for IFP–wt) and the shape of Ix/x vs x plot Specifically, the initial slow rise of the latter plot (from x ¼ 1) reflects that the self-association of the peptide is not tight (on the scale of...
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Tài liệu Báo cáo khoa học: "Syntactic and Semantic Transfer with F-Structures*" pptx
Ngày tải lên :
20/02/2014, 18:20
... Multiple Adjuncts Consider the sentences in (6) (6) a Oft kocht Hans gerne b Hans kocht gerne oft c Often Hans likes cooking d Hans likes cooking often (8) (6a) is ambiguous between (6c) and (6d), ... [PRED OFT][~] [PREDOE.NE ['4] ] a { like(n4), SUBJ(n4,n2), Hans(n2), XC0MP(n4,nl), cook(nl), SUBJ(nl ,n2), ADJN(nl,n3), often(n3) } LADJN {[paED OFT~.]Sl)J IT :J x[~] Hans(x~]) I ADJN(nl,n3), oft(n3), ... koehen(x~) I 344 { oft(E) x[] Hons()l lT:lHans(x~) I lT :I x[] (9) (ADJN(E,X) } { ADJN(E,X) ] ]- { often(E) } ! (8a) corresponds to only one of the English translations, namely (6d), of (6a) As...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
Ngày tải lên :
21/02/2014, 01:21
... Expression of SmGSTO was also studied at the transcriptional level by RT -PCR using cDNA prepared from miracidia, sporocysts, cercariae and adult worms (Fig 5B) A unique amplification product of the ... transcriptase (Life Technologies) in 50 lL of supplied reaction buffer PCRs were performed on lL of each reverse transcription reaction and resolved by agarose gel electrophoresis Primers used were: ... identity with the GST-like domain of the T cruzi protein A fragment of 549 bp was amplified by PCR using specific primers, designed based on the EST sequence, and cDNA from S mansoni adult worms The...
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Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt
Ngày tải lên :
07/03/2014, 05:20
... Fluorescence emission spectra of CTC in the absence (black line) and presence of increasing concentrations of bis-ANS (B) The efficiency of energy transfer from Tyr to bis-ANS (C) Data from the spectra shown ... fibrils [5] This property of SP-C appears to be relevant to human disease The fibrillar form of SP-C has been isolated from lung lavage fluid obtained from patients suffering from pulmonary alveolar ... were analyzed, 0.5 lL of sample was deposited on top of a thin layer of sinapinic acid precrystallized from a 30 mgÆmL)1 solution in acetone, and cocrystallized with 0.5 lL of sinapinic acid (30...
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Báo cáo Y học: Properties of the Na+/H+ exchanger protein Detergent-resistant aggregation and membrane microdistribution potx
Ngày tải lên :
08/03/2014, 16:20
... amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top of the gels The amount of aggregate present at the top of the Western blot of the gels ... Properties of the Na+/H+ exchanger (Eur J Biochem 269) 4891 Fig Distribution of caveolin and the Na+/H+ exchanger in membrane subfractions from AP-1 cells transfected with the NHE1 isoform of the ... monoclonal antibody of fractions from methyl b-cyclodextrin-treated cells (D) Immunoblotting with anti-caveolin Ig of membrane fractions from methyl b-cyclodextrin-treated cells of the Na+/H+ exchanger,...
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Báo cáo " Sol-gel synthesis and particle size characterization of CdSe Quantum dots " ppt
Ngày tải lên :
14/03/2014, 13:20
... diameter of about nm Fig TEM image of CdSe QDs A typical XRD pattern from the prepared CdSe nanoparticles and the positions of the Xray peaks for CdSe FCC are shown in Fig All the diffraction peaks from ... the mean sizes of the CdSe QDs from the peak width at half-maximum Particle sizes obtained from the width of the (111) diffraction are depicted in the table Table Particle size of samples with ... increased from to 10 minutes, the peaks become broadening but not very considerably The mean sizes of samples obtained from XRD patterns are smaller than those of these samples obtained from TEM...
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Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain" doc
Ngày tải lên :
14/03/2014, 22:20
... monitored the fluorescence of the four proteins in the presence of anionic LUVs as a function of pH The kmax of C shifted from 338 nm to 343 nm between pH 4.9 and 4.1, and then from 343 to 340 nm between ... fluorescent dye release from LUVs (Fig 4D) On the basis of all of the data, we describe the succession of steps leading to membrane binding and membrane penetration of both domains of the protein In ... coefficient of 2.0 For C, the parameters are: pK1 ⁄ = 5.15 and a Hill coefficient of 2.7 For CTW206 ⁄ 281F, the parameters are: pK1 ⁄ = 5.8 and a Hill coefficient of 1.9 (B) Quenching of Trp fluorescence of...
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Chapter 26 Remote Logging, Electronic Mail, and File Transfer pot
Ngày tải lên :
15/03/2014, 12:20
... example of suboption negotiation In this example, the client wants to negotiate the type of the terminal 26.13 Figure 26.5 Example of suboption negotiation 26.14 26-2 ELECTRONIC MAIL One of the ... WAN, we need two UAs, two pairs of MTAs and a pair of MAAs This is the most common situation today 26.24 Figure 26.11 Services of user agent 26.25 Note Some examples of command-driven user agents ... Figure 26.20 The exchange of commands and responses in POP3 26.45 26-3 FILE TRANSFER Transferring files from one computer to another is one of the most common tasks expected from a networking or internetworking...
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Cadmium and zinc interactions and their transfer in
Ngày tải lên :
15/03/2014, 23:22
... levels of these metals in the tissues of two plants from WA was significantly different from those from others by LSD at P- 0.05 Trace elements concerned were markedly different Table Contents of ... contents of soil metals concerned, particularly of soil Cd, had shown a considerable amount of pollution ŽNan and Zhao, 2000 The sampling region consisted of three areas according to the types of irrigation ... bags for analysis Two grams of crop Žroot, stem and grain sample was dissolved in the m ixture of H O ᎐HClO4 ᎐HNO3 , and 0.5 g of air-dried soil sam ple in the m ixture of H Cl ᎐ HNO3 ᎐HClO4 ᎐HF...
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