protocols for nuclear transfer in mice pdf

... when assessing lava flow hazard at the ANPP site Results and Discussion Using spatial density estimation Locating the source region of erupting lava is critical in determining the area inundated ... not influence the probability of occurrence of another event We are interested in constructing a spatial density model only using independent events Unfortunately, it is difficult to determine ... number of grid cells, thus increasing computation time as the flow has to pass through an increasing number of grid cells A balance needs to be maintained between capturing important flow detail...

Ngày tải lên: 21/06/2014, 19:20

... method for porcine cloning by nuclear transfer using in vitro-matured oocytes Cloning Stem Cells 2002, 4, 105-112 38 Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS Viable offspring derived ... medium for oocyte manipulation was calcium-free TLH-BSA containing µg/ml cytochalasin B After 40 h of maturation culture, denuded oocytes were incubated for 15 in a manipulation medium that contained ... NepaGene, Japan) The oocytes were incubated for h in TLH-BSA and examined for fusion under a stereomicroscope prior to activation Autologous somatic cell nuclear transfer in pigs 417 Activation and...

Ngày tải lên: 07/08/2014, 20:23

... rate of inbreeding when there is selection within families, but delays inbreeding for three or so generations in populations of this size and minimises the variation in inbreeding level within the ... population formed by crosses of inbred lines of mice selected for body weight, and therefore lines were probably not in linkage equilibrium when selection began A selection programme in mice was ... population before generation and maintained in the same way as these C lines, although they were initially used as controls for the lines selected for appetite [28] Results are given in Table V,...

Ngày tải lên: 09/08/2014, 18:21

... vaccine for preventing H5N1 virus infection in human Currently, two classes of drugs are available with antiviral activity against influenza viruses: the M2 inhibitors (amantadine and rimantadine), ... performed in a bio-safety level (BSL-3) laboratory Antiserum An inactivated influenza H5N1 vaccine strain isolated in 2003, provided by the South China Agricultural University, was performed for ... genetics for rapid development of influenza vaccines Lancet 2004, 363:1099-103 Wood JM, Robertson JS: From lethal virus to life-saving vaccine: developing inactivated vaccines for pandemic influenza...

Ngày tải lên: 12/08/2014, 16:20

... days after inoculation Mice were monitored for evidence of tumor growth by palpation and inspection twice a week For determining of tumor volume, each individual tumor size was measured Line and ... Birnstiel ML: Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene -transfer vehicle ... conjugated to alkaline phosphatase (Sigma, St Louis, MO) in secondary antibody solution Color was developed by incubating the membrane in alkaline phosphate buffer containing NBT (nitro blue...

Ngày tải lên: 14/08/2014, 19:22

... materials with interesting results To begin with, some simplifying assumptions were made Phonetic data were treated as a linear string by simply ignoring their very real grouping properties Furthermore, ... languages in Lower California and around the Colorado River delta His list of around 600 words collected in Paipai was prepared for process- 74 ing Phonetic symbols were transliterated into strings in ... is long A change in the pattern-recognizing approach is being considered to segregate phones at particular positions in syllables, syllables in feet, and feet in contours The intermediate storage...

Ngày tải lên: 16/03/2014, 19:20

... 120 postinjection through the tail vein, and glucose levels were measured Determination of plasma insulin and leptin levels Insulin and leptin levels in the plasma of mice were determined spectrophotometrically ... C57BL/6 mice fed western-type diet for 15 weeks (Fig 5) Interestingly, in our studies, feeding western-type diet to C57BL/6 for 15 weeks did not result in insulin resistance or glucose intolerance ... and analyzed for insulin and leptin At week 0, all three mouse groups had similar plasma insulin levels (0.17 ± 0.05 ngÆmL)1 for ApoE3knock -in mice, 0.12 ± 0.05 ngÆmL)1 for ApoE)/) mice, and 0.16...

Ngày tải lên: 17/03/2014, 17:20

... to increase the rate of innovation in the housing industry and (2) this can be accomplished by identifying, understanding, and removing barriers to innovation, increasing dissemination of information, ... Technology roadmapping identifies industry needs by brainstorming R&D planning processes to find starting points for planning federal and private R&D investments The PATH Industry Steering Committee, ... by identifying, understanding, and removing barriers to innovation, disseminating information to all participants in the housing industry, and undertaking research itself and facilitating privately...

Ngày tải lên: 22/03/2014, 10:20

... roles in initial evaluation and staging of patients, ART initiation, and patient monitoring [3] As nurses are becoming increasingly central points of contact for clinical care of people living ... Task shifting has lead nurses to be heavily involved in performing HIV testing and counselling, assessing patients for ART eligibility, assessing toxicity and treatment failure, and providing patient ... learning objectives for instruction are derived If integrating a new theme into an existing curriculum, these learning objectives can then be mapped to existing courses Then, appropriate teaching...

Ngày tải lên: 18/06/2014, 17:20

... originally trained through the train-the-trainer mechanism have identified and trained additional individuals The original cohorts of trainees are now conducting trainings themselves targeting ... sustainable change [2,11,16,17] Provide train-the-trainer sessions for in- country institution faculty who will teach the training in the future As each successive training round entailed increased ... ratings (% reporting “extremely well”) How well did the training: How well did the training meet its objectives for: Faculty’s ability to teach and manage the training (% reporting “yes, definitely”)...

Ngày tải lên: 18/06/2014, 17:20

... rule in closed-loop (Figure 1) Why we need explicit computerized protocols for mechanical ventilation? The human brain has a limited ability to incorporate data and information in decision making ... and to be clinically relevant before being sent to the control unit [21] The key point is to input relevant, valid, robust, and stable data for the ECP For example, the weaning ECP for children ... receives clinical information from the patient (input data) and “transforms” this information into orders (output data) The basic structure for processing information is rule-based (see above) All potential...

Ngày tải lên: 18/06/2014, 18:20

... glycoprotein synthesis was occurring in ganglia transplanted during acute infection and in ganglia transplanted following reactivation was obtained by performing immunohistochemical staining for HSV-1 ... BALB/c scid mice receiving ganglion grafts containing 10 PFU and of 10 mice receiving grafts containing PFU died within 14 days of receiving the grafts (Table 3) None of the 10 animals receiving the ... sections were stained with hematoxylin and eosin for histopathologic examination Additional sections were stained for cells expressing HSV-1 antigens A direct staining method employing a polyclonal,...

Ngày tải lên: 19/06/2014, 08:20

... undercutting large branches before pruning can eliminate bark tearing • Selecting branches for form pruning; • When to use tip pruning and how to apply it The participants then spent 30 minutes ... practising form pruning and familiarising themselves with the use of the various tools (Plates 2-4) Stand 2: Singling and 1st-lift pruning (Mr Trieu) • Singling and the consequences of not singling ... wood (shavings etc) The participants were able to see all aspects of the operation: rough sawing, drying, measuring and cutting piece sizes; final shaping, planing and form work; drilling, assembly...

Ngày tải lên: 21/06/2014, 06:20

... exploited for combining RTT information measured by all NodeBs in the AS (active set), thus improving the overall Cell ID+RTT accuracy According to regular SHO procedure [35], the radio link is ... constituting a performance measure in respect to the ith NodeB, as expressed in (2) The penalty function is introduced in order to form an applicable solution by employing an unconstrained LS ... and buildings, multiple positioning regions were defined within the same street or square in order to maintain the intended average size of the positioning region RSCP samples required for the...

Ngày tải lên: 22/06/2014, 22:20

... [38] Alizarin red-S and alcian blue staining were used to examine skeletal malformations [9] Results An increase in mortality was seen in the study on dose-incidence response, but the increase ... checked for external malformations under dissection microscope Fetuses were fixed in Bouin's solution, then stored in 70% ethanol The presence of visceral malformations was determined using Wilson's ... 28(100) Malformed offspring 9(9) External malformation Fetus examined Omphalocele Kinky tail Brachyury Club foot Digits Dwarf Anal atresia Hematoma Internal malformation Fetuses examined Dilatation...

Ngày tải lên: 07/08/2014, 15:20

... Linear Model Procedure; SAS ver 6.04, U.S.A.) Results The experiment was performed to examine whether or not CLA in combination with vitamin A (retinol) or selenium enforce the immunoglobulin ... consequences in the animals Effects of Combination Dietary Conjugated Linoleic Acid with Vitamin A (Retinol) and Selenium on the Response of the Immunoglobulin Production in Mice 107 In this experiment, ... immunoglobulin G in vivo is to promote the removal of microorganisms and neutralize toxins [11] Serum immunoglobulin E is an immunoglobulin of major importance in mechanisms against parasites and in the...

Ngày tải lên: 07/08/2014, 17:22

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Ngày tải lên: 07/08/2014, 18:21

... thank Gwendoline JD Teske and Walter Pouwels for technical assistance, Marjolein Vinkenoog for performing the histological stainings, Dr Maarten C Kraan and Dr Huib J Dinant for scoring the x-rays, ... shown In contrast to human CD97, murine CD97 lacks an RGD motif in the stalk region [27,28] which facilitates integrin binding in humans [10] The 1B2 mAb used in this study blocks binding to ... Brisbane, CA, USA) For F4/80 staining, antigen retrieval was performed by heating the sections for minutes at 95°C in 0.1 M citric acid at pH 6.0 Slides were preincubated with PBS containing 10% normal...

Ngày tải lên: 09/08/2014, 08:22

... resource allocation in lactating mice 91 A was significantly higher in the S-line than in the C-line (P < 0.001) Estimates of mature food intake (MFI in g/d) were 4.66 ± 0.0306 for C-line females and ... significantly higher in the S-line than in the C-line; in standardised litters this was significantly higher in the C-line than in the S-line The C-line pups opened their eyes earlier than the S-line pups ... demand during pregnancy and especially lactation Indeed, an increased energy for maintenance with selection for heat loss in mice allowed for a greater litter size as a correlated effect in the...

Ngày tải lên: 09/08/2014, 18:21

... breeding station in Denmark The experiment started in 1979 from a base population formed from a fast growing line of White Cornish origin, which had undergone selection for body weight since ... expecting to impair reproduction were culled Breeding animals were kept individually in cages and females were artificially inseminated In order to keep a generation interval of 11 months, eggs for ... covariance matrices and their inverses for models allowing for dominance and inbreeding Gen Sel Evol 22, 65-91 Sorensen P (1984) Selection for improved feed efficiency in broilers Ann Agric Fenn...

Ngày tải lên: 09/08/2014, 18:22