proteomic analysis of human mesenchymal stem cells
Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... used were from Sigma-Aldrich (St Louis, MO, USA), unless stated otherwise Culture of human mesenchymal stem cells Human MSCs were obtained from two sources, the first one being a commercially available ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2...
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... with human mesenchymal stem cells Tissue Eng 2002, 8:131-144 24 Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells ... Research (IZKF) Würzburg References Kolf CM, Cho E, Tuan RS: Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, selfrenewal and differentiation Arthritis Res Ther...
Ngày tải lên: 09/08/2014, 14:22
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... 53(7):1721-1732 Page 10 of 10 doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through...
Ngày tải lên: 10/08/2014, 05:21
Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... there are issues raised on accessibility of tissue sites where the mature cells can be harvested [23-30] Among several types of stem cells, mesenchymal stem cells (MSCs) have been shown to be an...
Ngày tải lên: 09/09/2015, 10:07
Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, ... differentiation of rat bone marrow-derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells using...
Ngày tải lên: 11/09/2015, 09:16
Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... 3.16 Statistical Analysis 87 Chapter – Coculture of Human Umbilical Cord Blood Endothelial Colony-Forming Cells with Human Fetal Mesenchymal Stem Cells for the Generation of Vascularised ... Chong, Z Zhang, MA Choolani, SH Teoh Coculture of human endothelial progenitor stem cells and bone marrow-derived human fetal mesenchymal stem cells potentiates osteogenesis through paracrine...
Ngày tải lên: 09/09/2015, 10:16
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...
Ngày tải lên: 31/10/2012, 16:57
Báo cáo y học: "ene expression analysis of human red blood cells"
... 1: Analysis of total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of ... different donors; B) typical electropherogram of total RNA of human RBCs Microarray analysis of RNA from human RBCs Recent proteomic studies of RBCs based on 1D/2D-electrophoresis 6, or mass spectrometry ... mechanism of globin m-RNA stability in human RBCs Further experiments are needed to understand the mechanisms and the biological meaning of these findings But gene expression profiling of human erythrocyte...
Ngày tải lên: 03/11/2012, 10:58
Atlas of Human Pluripotent Stem Cells doc
... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...
Ngày tải lên: 22/03/2014, 09:20
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx
... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number...
Ngày tải lên: 20/06/2014, 00:20
o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx
... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... is critical for self-renewal of human multipotent adipose-derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin ... homing of human mesenchymal stem cells at exposed sites but promotes their widespread engraftment to multiple organs: a study of their quantitative distribution after irradiation damage Stem Cells...
Ngày tải lên: 09/08/2014, 10:20
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...
Ngày tải lên: 11/08/2014, 12:21
Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen-induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... immunomodulatory activity of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow stromal cells inhibit allogeneic...
Ngày tải lên: 12/08/2014, 11:23
Báo cáo y học: "Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase-related genes in nucleus pulposus cells in rabbits" potx
... Induction of intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 23 Richardson S, Hughes N, Hunt J, Freemont A, Hoyland J: Human mesenchymal stem cell ... A, Sekiya I: Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum Arthritis ... http://arthritis-research.com/content/12/6/R206 Page of 13 Figure Synovial MSCs affect gene profile of nucleus pulposus cells in co-culture system (a) Morphology of mono-culture of rat nucleus pulposus cells and human synovial MSCs...
Ngày tải lên: 12/08/2014, 15:22
Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... cell-mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of human mesenchymal stem cells with disc cells: ... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior of mesenchymal stem cells in the chemical ... E, Bertram H, Abel R, Chen B, Winter A, Richter W: Induction of intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 11 Yoshikawa T, Ueda Y, Miyazaki K,...
Ngày tải lên: 12/08/2014, 15:23
Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... H, Baker SC, et al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell-derived NK cells acquire functional receptors ... the presence of CD34+/ KDR+ bipotential cells in non -human (Cynomolgus) ESCs Kennedy et al [20] recently reported the generation of BLCFCs from hESCs However, the rarity of the cells with hemangioblast...
Ngày tải lên: 14/08/2014, 08:20
Báo cáo y học: "Large-scale and high-confidence proteomic analysis of human seminal plasma" pptx
... Figure GoMiner analysis of the molecular function of identified proteins GoMiner analysis of the molecular function of identified proteins High-confidence and high-coverage analysis of the seminal ... tasks of the spermatozoa [31] The potential use of the proteomic data set for biomarker discovery Identification of disease biomarkers is an overarching aim of large proteomics studies of bodily ... database of the Department of Proteomics and Cell Signaling of the Max-Planck-Institut for Biochemistry [41] LC-MS3 The nano-high-pressure analysis was performed on an Agilent 1100 nanoflow system...
Ngày tải lên: 14/08/2014, 16:21