protein stabilization in the dried solid state

... domain or full-length MSR results in a quenching of the intrinsic flavin fluorescence, suggesting that the flavin chromophore is shielded from the solvent in the protein protein complex [19] From the ... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... footnote) in the reaction mixture may reduce a proportion of the FMN domain, converting some of the enzyme to the active form The  3-fold increase in activity may arise from the binding of the FMN...

Ngày tải lên: 18/02/2014, 08:20

... of the heparin-binding site in these three crystallized protein heparin complexes were given as starting point for the docking search [30] At least one conformation of the simulated protein peptide ... tubes containing either heparin or buffer Monitoring of FVa activity in these two tubes was continued for another 25 min, during which time the loss of FVa activity in the absence of heparin (open ... obtained in the presence of heparin (closed circles) This indicates that the presence of heparin did not in uence the k306 in the partially active FVa intermediate In a second experiment, the...

Ngày tải lên: 19/02/2014, 13:20

... hydration of the protein domain is the driving force of protein stabilization or folding [3,4] Furthermore, the effect of osmolytes on the functional activity of an enzyme has also been investigated ... references therein] The binding model claims that an increase in the osmolyte-induced stability arises from the preferential hydration (or exclusion of the osmolyte) leading to a shift in the denaturation ... towards the most compact protein species within native state ensembles [32,37,38] The variation in the effect of stabilizing osmolytes in modulating the catalytic efficiency of RNase-A in the presence...

Ngày tải lên: 07/03/2014, 00:20

... death inducer obliterator protein (DIDO), PHF3 and yeast proteins Additional proteins were included Naming conventions are as in Fig S1 The solid purple box indicates the TFS2M domain Pink-boxed ... maintain transcription The PHF3 protein was recovered in initial analyses and also contains a TFS2M domain showing the same architecture as the DIDO1 long isoform, although lacking the dPHD The ... set of DIDO1 homologues in the context of DNA binding and chromatin regulation These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated...

Ngày tải lên: 07/03/2014, 21:20

... PtdIns(4)P, PtdIns(5)P, PtdIns(3,4)P2 and PtdIns(3,5)P2 with the strongest binding to PtdIns(5)P (Fig 1) The ORD domain showed a very similar lipid binding pattern as the fulllength protein While the coiled-coil ... of the yeast oxysterol-binding protein homologs Genetics 157, 1117–1140 Beh CT & Rine J (2004) A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular ... lipid binding and a putative coiled-coil motif for protein protein interaction In this study, Osh6p was demonstrated to bind a pool of phosphatidylinositol phosphates (PtdInsP) including PtdIns(4)P,...

Ngày tải lên: 07/03/2014, 21:20

... repeatedly in studies of the oligomerization of GPCRs and other protein protein interactions [34–40] In these studies, BRET has been measured using Rluc and enhanced yellow fluorescent protein (EYFP) ... However, as these fusion proteins were retained in vesicles inside of the cells, we were not able to use them in the BRET studies It would have been interesting to tag Rluc and GFP molecules to the i2 ... agonist-induced BRET observed in this study is that the translation of agonist binding to the ATDs of the family C GPCR homodimer into G -protein coupling of the 7TM)7TM moiety is mediated by another...

Ngày tải lên: 08/03/2014, 09:20

... Poisoning the Well: How the EPA is Ignoring Atrazine Contamination in Surface and Drinking Water in the Central United States An Atrazine Primer Atrazine is a selective herbicide ... chemicals Poisoning the Well: How the EPA is Ignoring Atrazine Contamination in Surface and Drinking Water in the Central United States Recommendations for Protecting Human Health and the Environment ... atrazine as well as the presence of atrazine in drinking water Under the Safe Drinking Water Act (SDWA), the EPA has determined that no more than parts per billion (ppb) of atrazine (as a running...

Ngày tải lên: 15/03/2014, 10:20

... subdomain 1, where the spin probe is attached, is not directly involved in the intermonomer cross-link, because one of the cross-linking sites is occupied by the spin probe, therefore, the rigidity ... complexes the motion of the probe is less restricted relative to the strong binding state and there is no difference between the two weak binding complexes In the case of cross-linked F-actin, the ... temperature increase produce changes in the conformation of interacting proteins within the same temperature range Indeed, the hyperfine splitting constant decreases as the temperature is increased,...

Ngày tải lên: 16/03/2014, 13:20

... encoding the KPI domain and the lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus and mammalian APLP2 proteins Comparing the amino acid sequences of the two ... mammalian proteins are 91% and 100% identical, respectively, including the GYENPTY sequence that is present in all APP superfamily members and is involved in the intracellular routing of the proteins ... (Fig 1) All 12 cysteine residues in the aminoterminal part of APLP2 are present in X-APLP2 The zinc-binding domain consensus sequence (GxExVCCP [13]) in the N-terminal part of APLP2 is conserved...

Ngày tải lên: 16/03/2014, 16:20

... demonstrate that dexamethasone is an inducer of C/EBPa, exerting a positive effect on the steady -state levels of both the mRNA and protein levels in the liver The increase in the levels of both hepatic ... performed using an anti-C/EBPa Ig Symbol ÔsÕ in panel B and C denotes the position of the C/EBP containing complex and the arrowhead ÔssÕ the position of the DNA protein complex shifted by the specific ... transferred to poly(vinylidene difluoride) membranes, and the membranes incubated with a specific anti-C/EBPa Ig Protein loading control using Amido Black staining of the membrane is shown in the bottom...

Ngày tải lên: 17/03/2014, 09:20

... a binding buffer containing 100 mM NaCl, and the 35 S-labeled proteins that bound the resin via the His6-fused proteins were eluted with the binding buffer containing 500 mM NaCl The bound proteins ... characterize the RNA-binding properties and the possible protein protein interactions of HCF152, we produced the protein and several truncated versions in bacteria, and analyzed its protein protein interactions ... the background The binding site was found to be located at the C-terminus of the protein although binding of the C-terminus half (CH) is only half of the full-length protein (Fig 3) Interestingly,...

Ngày tải lên: 17/03/2014, 10:20

... {A,} in Let G be the symmetry group describing the symmetries of the branch of states with higher symmetry Then the state {w"Ic}) is an invariant of G In the vicinity of the transition the states ... Besides the instabilities encountered in laser active media, there exists another class of instabilities in nonlinear optics These instabilities are included in passive optical media by shining in ... discrete, rather than continuous, as in our other examples The minima of qY in the ordered state will be discontinuously degenerate Specializing Eq (7.16) for the present case we find The contour lines...

Ngày tải lên: 17/03/2014, 14:58

... polycomb protein Pc2 and the nucleoporin RanBP2 [33] PIAS proteins PIAS proteins are characterized by a so-called SPRING (Siz ⁄ PIAS-RING) domain, and bind both ubc9 and substrate proteins However, they ... with the adenylation domain at the base, and the UFD and cysteine domains on both sides The catalytic cysteine residue lies in a long loop between the cysteine domain and the adenylation domain ... nearly all other proteins involved in the modification reaction It possesses at least five protein interaction sites (a) The a1 region contains the a1 helix and surrounding residues of the b1b2 loop...

Ngày tải lên: 30/03/2014, 04:20

... perspectives in archaeology enrich knowledge about the lives of people in the past and their interactions with people in other gender groups in their communities The early gender studies in archaeology ... are subdivided into those activities relating to acquiring and processing food; making and repairing tools, clothing, and buildings; and maintaining social relations For each step in an activity, ... contemplate whether it is sex or gender they are interested in investigating and how or whether sex data can be converted into gender data (Damm 1991) Gender-free Writing In addition to the assumptions...

Ngày tải lên: 11/06/2014, 13:10

... one-third of the patellar tendon (1 × mm) from the distal apex of the patellar to the insertion of the tibial tuberosity was then removed and the gap was left open The wound was then closed in layers ... Louis, MO) or saline were injected into the patellar tendon intratendinously with a 30G needle in one limb while the contralateral limb was injected with saline [6] Sample harvest The rats with ... Care therefore should be taken when interpreting the mRNA data and studying the expression also at the protein level by immunohistochemistry is suggested in tissue samples Figure calcific injury...

Ngày tải lên: 20/06/2014, 04:20

... water and dried in airflow The binary complexes were obtained by mixing 10 μl of μM solutions of appropriate individual proteins in 50 KP, pH 7.4 Then, the mixture was incubated for 10 min, diluted ... r(h)AdR/Ad for binary mixtures (d) Arrows (1) indicate the images of protein monomers Arrows (2) indicate the images of the binary protein complexes Arrows (3) indicate the images of the ternary ... described before [34] All protein samples were diluted in 10 mM KP (pH 7.4) Possible changes in the secondary structures of the proteins were investigated by recording CD spectra in the range of 195–260...

Ngày tải lên: 21/06/2014, 11:20

... ‘R,’” Southern Speech Journal 9: 102–6 Phliponneau, Catherine (ed.) 1995 Sociolinguistic Studies and Language Planning: Proceedings of the XVIth Annual Meeting of the Atlantic Provinces Linguistic ... “Investigating variation and change in written documents,” in Chambers, Trudgill, and Schilling-Estes (eds.), 67–96 forthcoming The English dialect heritage of the Southern United States,” in ... article: the Linguistic Atlas of the Gulf States,” American Speech 68: 263–318 1993b The Southern accent – alive and well,” Southern Cultures, inaugural issue: 47–64 1993c The verb be in American...

Ngày tải lên: 24/07/2014, 09:21

... $70,000 a year,” in her words In selling mailing lists over the telephone, she finds the strategy of switching into a southern-sounding way of talking and interacting to be particularly useful ... southern-sounding speech King may well sound southern in other contexts too, for other reasons, including ones such as those discussed above These examples illustrate just four points on a continuum ... southern style in her bid to “sound southern.” The resources of southernness are available to these women because of where they are from: they have heard people sounding like Southerners all their...

Ngày tải lên: 24/07/2014, 09:21

... about them, by outsiders and insiders This idea structures much discourse about Southerners, whether the Southerners in question are from the coastal or the mountain South and whether they are ... of the specific linguistic features which have been observed in the speech of some Southerners (and in literary and other representations of southern speech) Then I talk about some of the things ... “playback” phase in which she asked Southerners to comment on the meanings of these address terms while watching clips of the film Like Ching, she found that the core meaning was the expression...

Ngày tải lên: 24/07/2014, 09:21

... up in the pronunciation of the state name, with northern Louisiana favoring four syllables beginning [luz-] and southern Louisiana favoring five syllables beginning [luiz-] The people of southern ... declining since the last quarter of the nineteenth century, when the oldest cohort of LAGS informants was born The use of preterit come and plural verbal -s seems to have begun to decline in the ... fronting of /u/ in the vowel system of the Virginian, none of these systems show evidence of the Southern Shift In all three vowel systems, /e/ remains to the front of /ε/, both /o/ and /u/ remain...

Ngày tải lên: 24/07/2014, 09:21