protein molecular weight calculator mass spectrometry

Ngày tải lên: 24/08/2014, 09:55

... 39–46. 9 Sinz A (2006) Chemical cross-linking and mass spec- trometry to map three-dimensional protein structures and protein- protein interactions. Mass Spectrom Rev 25, 663–682. 10 Lutter LC & ... chemical cross-linking and mass spectrometry. J Mass Spectrom 43, 185–195. 14 Pimenova T, Pereira CP, Schaer DJ & Zenobi R (2009) Characterization of high molecular weight multimeric states ... mass spectrometry for protein interaction analysis: application to a calmodu- lin ⁄ target peptide complex. Anal Chem 77, 495–503. 19 Tang XT, Munske GR, Siems WF & Bruce JE (2005) Mass spectrometry...

Ngày tải lên: 15/03/2014, 00:20

... activation of a low -molecular weight protein tyrosine phosphatase by adenine. Biochemistry 39, 1234–1242. 39 Xu H, Xia B & Jin C (2006) Solution structure of a low -molecular- weight protein tyrosine ... low molecular weight phosphotyrosine- protein phosphatase. J Biol Chem 271, 1278–1281. 10 Tailor P, Gilman J, Williams S, Couture C & Mustelin T (1997) Regulation of the low molecular weight phosphotyrosine ... [5], has also been Keywords low -molecular- weight protein tyrosine phosphatase (lmwPTP); phosphatase regulation; protein oligomerization; signaling pathways; supramolecular proenzyme Correspondence M....

Ngày tải lên: 16/03/2014, 02:20

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Ngày tải lên: 05/09/2013, 10:15

... Commun. Mass Spectrom. 2008; 22: 2993–2998 DOI: 10.1002/rcm 2994 L. Zhu et al. Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry Liang ... fast response. Mass spec- trometry-based methods are of particular interest for the on- line analysis of reactions, 1 due to their high sensitivity and high specificity. Tandem mass spectrometry ... organic chemistry. 2,3 Although direct infusion electrospray ioniz- ation spectrometry (ESI-MS) 4–9 and membrane introduction mass spectrometry (MIMS) 10–12 are gaining popularity in this field, both...

Ngày tải lên: 14/02/2014, 08:20

... protein identification result. Using the iTRAQ labeling mass spectrometry, a total of 438 proteins were identified by our proteomic platform. To better understand the roles of these identified proteins, ... eight patients. Functional classification of proteins identified in CRC Proteins identified by mass spectrometry were classified by subcellular location and molecular function (Fig. 3). To better understand ... S100A8 and S100A9. Figure 3B shows the molecular function categorization of the proteins iden- tified in CRC patients. Regarding major molecular functions, the proteins were mostly associated with binding...

Ngày tải lên: 16/02/2014, 15:20

... of ApoC1 as well as some protein properties that suggest the functional significance of this residue change. They Keywords apolipoprotein C1; mass spectrometry; polymorphism; protein lipid contact ... inten- sity ratios to estimate relative protein abundance depends on equal crystallization of the protein in the matrix and equal ionization of the proteins in the mass spectrometer. This assumption ... polymorphism of apolipoprotein C1 FEBS Journal 273 (2006) 4707–4715 ª 2006 The Authors Journal compilation ª 2006 FEBS 4711 A functional polymorphism of apolipoprotein C1 detected by mass spectrometry Matthew...

Ngày tải lên: 19/02/2014, 05:20

... by MALDI-TOF MS. The measured molecular mass of the purified product was 11 799.70 ± 2, which concurs closely with activated (Pyr)-ONC (M23L), which has a theoretical molecular mass of 11 801.60. Contribution ... intensities of the peaks corresponding to Gln-ONC (M23L) (theoretical molecular mass 11 818.87) and Pyr-ONC (M23L) (theoretical molecular mass 11 801.60), and expressing it as the fraction of hydrolyzed (Met1)-ONC ... thermostability. Protein Expr. Purif. 17, 169–181. 19. Mozhaev, V.V., Heremans, K., Frank, J., Masson, P. & Balny, C. (1996) High pressure effects on protein structure and function. Proteins 24,...

Ngày tải lên: 19/02/2014, 12:20

... oligosaccharides by com- bined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry. J. Am. Soc. Mass Spectrom. 13, 670–679. 15. Domon, B. & Costello, ... (see below), having the same molecular mass with one Fuc in each of the 3- and 6-branches. Comparison of the product-ion spectra of the singly and doubly charged molecular ion precursor (Fig. ... contaminant in DFiLNO as the precursor (B). 1176 H. Kogelberg et al.(Eur. J. Biochem. 271) Ó FEBS 2004 mass spectrometry and 1 H-/ 13 C-nuclear magnetic resonance spectroscopy. Evidence for a new core...

Ngày tải lên: 19/02/2014, 12:20

... stereospecificity at C5 of coenzyme F 420 for F 420 H 2 oxidase from methanogenic Archaea as determined by mass spectrometry Henning Seedorf 1 ,Jo ă rg Kahnt 1 , Antonio J. Pierik 2 and Rudolf K. Thauer 1 1 ... nicotinamide adenine dinucleotide-linked oxidoreductases: electron impact and field desorption mass spectrometry. Anal Biochem 101, 413–420. 22 Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer ... of [5- 1 H]F 420 and [5- 2 H]F 420 as revealed by the relative intensities of the 772 and 773 Da mass peaks (Fig. 2Cc). The results are consistent with FprA catalyzing the oxidation of (5S)- [5- 2 H 1 ]F 420 H 2 to...

Ngày tải lên: 20/02/2014, 03:20

... Techniques for the Analysis of Organic Chemicals by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Petrochemical Authors Ed McCurdy & Don Potter Agilent Technologies ... Cheadle Royal Business Park Manchester, SK8 3GR UK Abstract Inductively Coupled Plasma Mass Spectrometry (ICP- MS) is used for the routine monitoring of trace and ultra-trace metal contaminants ... technique is available to any Agilent ICP-MS system fitted with the ShieldTorch System and a mass flow controller capable of adding oxygen to the plasma. In routine operation, automatic switching...

Ngày tải lên: 05/03/2014, 21:20

... in protein analysis because of its inherently ultra-high mass- resolving power and mass accuracy. For instance, protein ligand [57], protein carbohydrate [58,59], protein peptide [60], and protein RNA ... soft ionization mass spectrometry. Int J Mass Spectrom 216, 1–27. 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry. Mass Spectrom Rev 23, ... monoisotopic peak is often undetectable for proteins > 10 kDa, except for isotope-depleted proteins [67]. Hence, the molecular masses reported hereafter refer to the masses calcula- ted from the most...

Ngày tải lên: 07/03/2014, 17:20

... least reliable mass isotopomer fractions were considered in the flux analysis. Discussion In the work described, we examined the extent to which mass isotopomer measurements of biomass hydrolysates, after ... time profiles of the (M + 0) and (M + 1) mass isotopomer fractions of selected tributyl dimethyl silyl (TBDMS)-amino acid fragments. M depicts the molecular weight of a fragment, i.e. all its atoms ... and PEP Fig. 3. Time profiles of the M + 0 and M + 1 mass isotopomer fractions of selected TBDMS-amino acid fragments. Mdenotesthe molecular weight of a fragment, i.e. all its atoms are in their...

Ngày tải lên: 08/03/2014, 08:20

... model; native mass spectrometry; protein RNA binding; protein RNA cleavage Correspondence R. Dı ´ az-Orejas, Centro de Investigaciones Biolo ´ gicas, Departamento de Microbiologı ´ a Molecular, ... the Gaussian charge state envelope of the bound protein. The percentage of protein bound to RNA was the ratio between the value of bound protein and the total protein present in the sample. The relative ... analysis of macromolecular complexes. These protein complexes are detected at different mass- to-charge ratios ( m ⁄ z), separated by differences in their time-of-flight inside the mass analyser....

Ngày tải lên: 16/03/2014, 03:20

... corresponding to alternative sites of proteolysis; one fragment has a molecular mass of 32582 Da and the second has a molecular mass of 32739 Da. As expected, after incubation with methyl- malonyl-CoA, ... the ACP-TE di-domain showed two peaks, one with a molecular mass of 39506 Da corresponding to the unloaded form and the other with a molecular mass of 39604 Da, cor- responding to the form loaded ... derivative. AT ACP-TE Methyl- malonyl adduct Unloaded forms Methyl- malonyl adducts Unloaded form adduct 33 200 32 80032 400 39 500 39 600 39 70039 400 Mass (Da) Mass (Da) Fig. 3. The electrospray mass spectra pro- duced by the AT and ACP-TE fractions derived from the extension...

Ngày tải lên: 16/03/2014, 03:20

... treatment, indicating that an intramolecular disulfide bond might have formed within the loading didomain (the mass accuracy for the 56 kDa protein would not allow us to detect the 2 Da mass decrease due to ... based on accessible and simple mass spectrometry equipment rather than advanced FT-ICR-MS. Nevertheless, the technology can achieve mass accuracy within 0.01% for proteins up to 79 kDa and so can ... release isolated domains even when the protein was treated with up to 2 m urea with the aim of partially unfolding the protein. To check whether other proteinases could digest module 2, ela- stase...

Ngày tải lên: 16/03/2014, 18:20