protein concentration and peptide pattern

... GLP-2, PHM, GRF and GIP GLP-1 and GLP-2 are similar peptides, with molecular masses of 3299 Da and 3768 Da, respectively Both peptides have His-Ala as the N-terminal dipeptide, and DPP4 cleavage ... producing peptides of 3090 Da and 3558 Da for GLP-1 and GLP-2, respectively Both peptides were inefficient FAP substrates, with half-lives of 22 h and 19 h, respectively (Fig 5) PHM and GRF were ... proteases, namely the cell surface glycoproteins DPP4 (EC 3.4.14.5) and fibroblast activation protein- a (FAP), and the intracellular proteases dipeptidyl peptidase (DPP8) and dipeptidyl peptidase (DPP9)...

Ngày tải lên: 14/03/2014, 23:20

... 20-fold and fourfold enrichments in the cell interior for the MAP-conjugate and the naked PNA, respectively (related to the external concentration and a ratio of about 10 lL cell volumeÆmg protein) 1 ... FACS and CLSM by providing absolute quantitation of internalized PNA CLSM revealed extensive fluorescence in the cytosol and nucleus of CM and CHO cells after exposure to both the naked PNA I and ... the peptide tag and the relatively long time required for the wash and trypsination processes, respectively, might have biased the results In addition to the information provided by CLSM and...

Ngày tải lên: 23/03/2014, 13:20

... L1 (H76-H78 and protein L1) and the L7/L12 stalks (H43-H44, the loop of H95, called also the sarcin-ricin site, and proteins L10, L11 and L12) H76-H78 are directly connected to H75, and, from the ... strandsÕ) and the A- and the P-loops (that are the stem loops of helices H80 and H92) The second (middle) shell includes helices H80 and H92, the stems of the A- and the P-loops, H74 and H90 The outer ... with protein L32 and with helices H35 and H35a, TAO introduces striking conformational rearrangements in the exit tunnel [60] by flipping the tip of the b-hairpin of protein L22 (Fig 6) Protein...

Ngày tải lên: 23/03/2014, 17:22

... concentration was between 100 and 2000 pg/mL Plot a standard curve of absorbance as a function of initial protein concentration and use it to determine the unknown protein concentrations (see Notes ... at 1N concentration Standards: Use a stock solution of standard protein (e.g., bovine serum albumin fraction V) containing mg/mL protein in distilled water stored frozen at -2OOC Prepare standards ... is a function of protein concentration and incubation time, the protein content of unknown samples may be determined spectrophotometrically by comparison with known protein standards A further...

Ngày tải lên: 10/04/2014, 11:03

... procedures generally used m protein chemistry m terms of senstttvtty and acceptance of solvents, detergents, and buffers, They must be able to handle impure samples and complex mixtures Last, ... K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry/Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York 16 Hogeland, K E , Jr and Demzer, M L (1994) ... the CID spectra and the presence of gaps m the fragmentation patterns vary greatly from peptide to peptide Generally, of course, the interpretabihty of the CID spectra of these peptides will decrease...

Ngày tải lên: 11/04/2014, 10:11

... metabolism-relevant ligands Click here data file bio-ligands Ligands and theand2 of bio-ligands and binding ATP, ligands Additionalfor file 1ownerships of metabolism-relevantADP and block numbers of for proteins ... ACD-SC-derived ordinary molecules; Ligands, sc-PDB-derived ligands; Ligands (≤ 3), ligands covering ≤ folds; Ligands (4-9), ligands covering 4-9 folds; Ligands (≥ 10), ligands covering ≥ 10 folds Implications ... ligand (L) Figure Power-law behaviors of ligand -protein binding Power-law behaviors of ligand -protein binding The number of ligands (N) decays with an increase in the number (L) of (a) domains and...

Ngày tải lên: 14/08/2014, 08:20

... of proteins Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the molecular weight (m/z) and relative concentration (intensity) of each protein ... cancer patients and healthy volunteers was collected and analyzed A panel of differentially expressed proteins was advocated for biomarkers of diagnosis for colorectal cancer Materials and methods ... mixture was aspirated and spotted onto the gold-coated ProteinChip array After air-drying for minutes at room temperature, protein crystals on the chip were scanned with the ProteinChip (Model PBS...

Ngày tải lên: 25/10/2012, 11:18

... become greater Most organic matter in water is being dissolved and consists of protein, carbohydrate, fat and humic substances and so on And furthermore, about 70 to 80% of total dissolved organic ... the middle and lower layer Namely, the tendency of statistics between DOC and UVA254 in middle layer and lower layer was similar As a result, condition of both DOC and UVA254 at middle and bottom ... through the whole period and that of middle and lower layers in wet season were 2.696 and 2.686 respectively REFERENCES Aki, S and T Shirasuna, (1973) Investigation and analysis of turbid flows...

Ngày tải lên: 05/09/2013, 08:40

... hóa protein thể:  Tiêu hóa hấp thu protein động vật dày đơn: Dưới tác động nhóm enzyme protease, protein thức ăn thủy phân thành polypeptide, oligopeptide cuối thành amino acid Sự thủy phân protein ... monomer cấu tạo nên protein Trong tự nhiên có khoảng 250 axit amin protein thể sống chứa 20 axit amin gọi axit amin sinh protein (proteinogenic amino acids) Hình dạng đặc tính protein quy định trình ... dạng liên kết peptide Mạch Mạch bên Hình ạch bên khung chuỗi polypeptide HƢƠNG QUÁ TRÌNH PHÂN GIẢI PROTEIN, AXIT AMIN, PEPTIDE 4.1 QUÁ TRÌNH PHÂN GIẢI PR TEIN Thủy phân đường phân giải protein phổ...

Ngày tải lên: 07/12/2013, 22:07

... (1979) and Viljoen and Du Toit (Viljoen 1981; Viljoen and Du Toit 1985), who studied litter size and growth rates in Epixerus, Funisciurus, Heliosciurus, Myosciurus, Paraxerus, and Protoxerus and ... al 1966; 16, Bhat and Mathew 1984; 17, Sahi and Sharma 2004; 18, Tien 1966; 19, Grinell and Storer 1924; 20, Stapp and Mautz 1991; 21, Tate 1947; 22, Thomas 1900; 23, Ando and Shiraishi 1984a; ... 54%; Figs 3C and 3D), and annual output (litter mass  litters/year) and maternal mass (n ¼ 40, P , 0.0005, R2 ¼ 70%; PIC: P , 0.0005, R2 ¼ 45%; Figs 3E and 3F) Only taxa, Sciurini and Marmotini,...

Ngày tải lên: 13/02/2014, 16:20

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into ... residues, and provided evidence that these novel protein protein interaction core modules play a pivotal role in regulating dendrite growth and branching of cerebellar granule cells and hippocampal ... study revealed the structural and functional determinants of the protein protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module...

Ngày tải lên: 14/02/2014, 19:20

... Rbx1, and one of a family of F-box proteins, Fbw7 [17] Glycogen synthase kinase-3b phosphorylates serine and ⁄ or threonine residues near the ubiquitination site in SREBP-1 and SREBP-2, and SREBP ... enzyme for bile acid synthesis, and apolipoprotein A-1, a high-density lipoprotein protein The basic helix–loop-helix leucine zipper domain in SREBPs binds to the ligand-binding domain in LRH-1, ... 2008 FEBS 625 Protein interaction and SREBPs R Sato ing proteins, SCAP and importin-b, play an important role in determining the fate of SREBPs In the nucleus, multiple nuclear proteins form...

Ngày tải lên: 18/02/2014, 13:20

... IgG-binding protein L and SH2/SH3 domains for binding to their ligands (IgG and a phosphotyrosyl peptide, respectively) [63–66] A variation on this idea was to combinatorially design proteins de ... behavior of stable, native-like proteins differs from unstructured polypeptides, and the consequences of this can be used to sort polypeptide libraries for native-like proteins We will discuss the ... what makes proteins ÔtickÕ and to engineer proteins with native-like properties In this review we discuss combinatorial approaches toward understanding protein structure and stability In the ideal...

Ngày tải lên: 19/02/2014, 12:20

... among the SEA modules of MUC1 glycoproteins from different species, and is found in other membrane-tethered mucins including MUC3, MUC12, MUC16 and MUC17 and other proteins with cleaved SEA domains ... consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold The FRPG ⁄ SVVV residues of the MUC1 cleavage site, which is located in a loop between b2 and b3 strands, are illustrated ... cells and clones expressing high levels of the hybrid glycoproteins identified Figure 4A and B show western blots of M2-immunoprecipitated MUC1FDTR ⁄ MUC3-CL and MUC1FDTR ⁄ MUC12-CL glycoproteins...

Ngày tải lên: 19/02/2014, 18:20

... extended peptides (TKRP-2 and -3) are present in both species and the other peptides are nona- or decapeptides, except LemTKRP-11 which is a heptapeptide It is also noteworthy that all the LemTKRPs and ... 13 peptides are nona- or decapeptides; only the unprocessed first and the last encoded peptide are significantly longer (TKRP-2, 18 amino acids and TKRP-3, 19 amino acids) In addition, a novel peptide ... PeaTKRPs The numbering of peptides follows that for already identified peptides [14,15], and not their sequence order on the precursors Thus novel peptides 3373 Tachykinin-related peptides in cockroaches...

Ngày tải lên: 20/02/2014, 01:20

... activities of GAPP and AMPK, although the involvement of other protein kinase or phosphatases has not been ruled out Because ACC and p70S6K display a similar and parallel pattern of activation ... to activate ACC and p70S6K After 50 min, NaCl (0.9% final concentration, n ), rapamycin (Rapa, 300 nM final concentration, ) or AICAr (0.5 mM final concentration, j) were added and the cells were ... protein kinase, and lack of activation by cyclic-AMP-dependent protein kinase, studied using a specific and sensitive peptide assay Eur J Biochem 186, 123–128 Marsin, A.S., Bertrand, L., Rider,...

Ngày tải lên: 22/02/2014, 07:20

... molecules with the level and function of UCP2 and free radical production in macrophages We find that MDP induces reactive oxygen and nitrogen species production and upregulates UCP2 protein level, whereas ... complexes I and III [34] The ROS generated in mitochondria are removed by local superoxide dismutases and peroxidases and by reaction with low molecular weight reductants and sulfhydryl-containing protein ... muramyl peptides and LPS on respiration rates and RCR in murine peritoneal macrophage mitochondria in vitro (A) Oxygen consumption was measured in the presence of 100 lgÆmL)1 of MDP after 1, 2, and...

Ngày tải lên: 05/03/2014, 23:20

... rate of protein synthesis, mutations and aberrant protein biogenesis, environmental (usually heat or oxidative) stress and heterologous protein production Ageing is mostly known to induce protein ... native-like proteins passively trapped in the aggregate and instead attributed this distribution to the specific mechanisms of protein deposition and removal, and further suggested that aggregated proteins ... young and with higher growth rate) and an IB-containing one that will grow more slowly [13] Potential of bacterial inclusion bodies Fig Protein biosynthesis and aggregation under normal and stress...

Ngày tải lên: 06/03/2014, 00:20

... amyloidogenic peptides (115 of 144 peptides) and 72% of non-amyloidogenic ones (189 of 263 peptides), with a cut-off value of 0.062, from a database of 407 amyloidogenic and non-amyloidogenic peptides ... solutions Out of 16 peptides syn- Fig A schematic example of how protein aggregation and amyloid fibril formation prediction software might be used for fine-tuning and control of protein solubility ... discussed in detail by Garcı´ a-Fruitos et al in this series, and also in [41] and mutational effects on the aggregation of peptides and proteins Nat Biotechnol 22, 1302–1306 Yoon S & Welsh WJ (2004)...

Ngày tải lên: 06/03/2014, 00:20

... and is a small regulatory protein that belongs to the PII protein family GlnK homologs bind to AmtB and regulate its activity, depending on the cellular nitrogen status [14] Like other GlnK proteins, ... exponential growth phase, and the cells were then washed and resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free ... various nucleotides (ATP, ADP, AMP, and GTP) and 2-OG at different concentrations on GlnK binding to TnrA GlnK–TnrA interaction in the presence of mm ATP On the other hand, 2-oxoglutarate did not influence...

Ngày tải lên: 06/03/2014, 00:21