protein bovine serum albumin

... chemistry of human serum albumin Nature 358: 209–214, 1992 DC Carter, JX Ho Structure of serum albumin Adv Protein Chem 45:155–203, 1994 A Kondo, K Higashitani Adsorption of model proteins with wide ... contact between a single protein molecule and the clay surface at different pH and ionic strengths Conformational Changes on Adsorption of a Soft Protein, Bovine Serum Albumin a Description of ... involved in interactions of proteins with hydrophilic mineral surfaces An example is given by the higher affinity of a hydrophobic methylated derivative of bovine serum albumin (BSA) for the hydrophilic...

Ngày tải lên: 11/08/2014, 15:20

... incubating protein with ligands in a : ratio Complex III was prepared by mixing the protein, NADP+ and inhibitor in a ratio of : : 10 Crystals were grown in hanging drops constructed from lL of protein ... > ⁄ Mosaicity (°) R-sym (%)a ˚ Wilson B (A2) No of protein residues ⁄ solvent molecules R-work b ⁄ R-free c (%) ˚ Average B (A2) Overall ⁄ protein ⁄ solvent rmsd ˚ Bond lengths (A) ⁄ bond angles ... Systematic analysis of conformational domain motions in proteins from conformational change: new results on citrate synthase and T4 lysozyme Proteins 30, 144–154 Hanau S, Dallocchio F & Rippa M...

Ngày tải lên: 19/02/2014, 05:20

... 8.0 Bound proteins were eluted by a linear gradient from to m NaCl in the same buffer Fractions containing PelB were pooled and concentrated (Filtron Technology Corp.; 30-kDa cut-off) Protein concentrations ... brisolvens from the bovine rumen J Gen Microbiol 131, 20532058 33 Wojciechowicz M, Heinrichova K & Ziolecki A (1982) An exopectate lyase of Butyrivibrio brisolvens from the bovine rumen J Gen ... treatment of the cell-free extract, followed by anionexchange chromatography, during which the protein was eluted at 0.6 m of NaCl Analysis of PelB by gel ltration resulted in a peak with an...

Ngày tải lên: 20/02/2014, 03:20

... TL (2006) Protein Protein interactions among Helicobacter pylori Cag proteins J Bacteriol 188, 4787–4800 Mossey P, Hudacek A & Das A (2010) Agrobacterium tumefaciens type IV secretion protein VirB3 ... CagA-positive strains, was shown to encode proteins with sequence similarity to Agrobacterium tumefaciens Vir proteins, and it was further demonstrated that these proteins are necessary for inducing ... compilation ª 2011 FEBS 1205 Cag protein functions W Fischer Table Overview of characteristics and functions of cagPAI-encoded proteins Gene designations and protein sizes are in accordance with...

Ngày tải lên: 06/03/2014, 00:21

... lacks four direct contacts with protein atoms made by the hydroxyl groups of the ribityl chain in the other structures Moreover, several direct contacts with the protein chain by the nitrogen atoms ... methods for building protein models in electron density maps and the location of errors in the model Acta Crystallogr A47, 110119 Kleywegt GJ & Jones TA (1998) Databases in protein crystallography ... (%) Overall I r Rsym overall (%)a Wilson plot (A2) Renement Resolution range (A) Non hydrogen protein atoms Non hydrogen inhibitor atoms Solvent molecules Solvent ions Rcryst overall (%)b Rfree...

Ngày tải lên: 07/03/2014, 11:20

... physiological role of the PAT2 transporter is currently not known We previously reported the PAT2 protein expression pattern in the mouse brain [10] and demonstrated that it is highly expressed ... possible insertion of PAT2 into the neuronal plasma membrane and we proposed PAT2 as a candidate protein for the still missing Na+-independent low affinity glycine transport system in the central ... other tissues than the nervous system, cellular and subcellular expression pattern of the PAT2 protein have not been examined so far, although the mRNA is expressed in various organs such as...

Ngày tải lên: 07/03/2014, 16:20

... For analysis of bound proteins, the last pellets were eluted using 0.2 M glucose, M NaCl, 1.5 M urea or H2O before loading onto SDS–polyacrylamide gels (10% acrylamide) for protein analysis and ... buffer and gently re-suspended for removal of unbound proteins A final wash was performed with 10 mM phosphate buffer pH 6.0 Elution of bound proteins was achieved by addition of vol water, followed ... terminator was found after the stop codon of cel48C, acting as a signal structure for protein synthesis termination The protein deduced from cel48C contained 1091 amino acids and showed a predicted molecular...

Ngày tải lên: 08/03/2014, 02:21

... indispensable for productive protein translocation Several of the mutated precursor proteins associate strongly with the membrane in the absence of efficient export It is possible that the proteins are associating ... References Cline K, Ettinger WF & Theg SM (1992) Protein- specific energy requirements for protein transport across or into thylakoid membranes Two lumenal proteins are transported in the absence of ... C, Berks BC & Palmer T (2000) TatD is a cytoplasmic protein with DNase activity No requirement for TatD family proteins in sec-independent protein export J Biol Chem 275, 16717–16722 10 Jack...

Ngày tải lên: 16/03/2014, 04:20

... mitochondrial trifunctional protein and its inactivation study for medicine development Biochim Biophys Acta 1784, 1742–1749 33 Fersht A (1999) Structure and Mechanism in Protein Science: A Guide ... Automated protein model building combined with iterative structure refinement Nat Struct Biol 6, 458–463 46 Jones TA, Zou J, Cowan SW & Kjeldgaard M (1991) Improved methods for building protein ... seen that the binding mode is the same as that for OPP; furthermore, the structural changes of the protein part described for the OPP complex are seen as well in the Ac-OPP complex Due to low occupancy...

Ngày tải lên: 16/03/2014, 04:20

... barley serine proteinase inhibitor Protein Eng 2, 27–38 32 Laskowski, R.A., MacArthur, M.W., Moss, D.S & J.M.Thornton (1993) PROCHECK: a program to check the stereochemical quality of protein structure ... constants in proteins Biochem Biophys Res Commun 113, 967–974 31 Nilges, M., Gronenborn, A.M., Brunger, A.T & Clore, G.M (1988) Determination of three-dimensional structures of proteins by simulated ... single-quantum NMR spectroscopy of aqueous solutions J Biomol NMR 2, 661–665 27 Wuthrich, K (1986) NMR of Proteins and Nucleic Acids John Wiley & Sons, New York 28 Davis, D.G & Bax, A (1985) Assignment...

Ngày tải lên: 17/03/2014, 11:20

Ngày tải lên: 17/03/2014, 19:20

... (1999) Miniaturized proteins: the backbone cyclic proteinomimetic approach J Mol Biol 292, 421429 32 Murray JK & Gellman SH (2007) Targeting protein protein interactions: lessons from p53 MDM2 Biopolymers ... protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expression Biochemistry 44, 1155511566 31 Kasher R, Oren DA, Barda Y & Gilon C (1999) Miniaturized proteins: ... tetramers and associates with LEDGF p75 protein in human cells J Biol Chem 278, 372381 45 Turlure F, Devroe E, Silver PA & Engelman A (2004) Human cell proteins and human immunodeciency virus...

Ngày tải lên: 22/03/2014, 16:21

... structure the two protein chains form a swapped dimer with two globular functional units which are composed of residues 1–35 from one protein chain and residues 38–65 from another protein chain (Figs ... DNA binding is dominated by stacking interactions between nucleobases and aromatic protein side chains for both proteins Based on this observation and on binding assays using a set of heptapyrimidines, ... amphipathic protein surface Several residues participating in ligand binding are located in regions designated as RNP motifs I and II, which can also be found in other RNA-binding proteins [26,27]...

Ngày tải lên: 23/03/2014, 09:21

... translocation of proteins across membranes Biochem J 347, 601–612 56 Nouwen N, Tommassen J & de Kruijff B (1994) Requirement for conformational flexibility in the signal sequence of precursor protein J ... antibacterial peptides Curr Drug Targets 3, 101–106 19 Otvos L Jr (2005) Antibacterial peptides and proteins with multiple cellular targets J Pept Sci 11, 697–706 20 Park CB, Yi KS, Matsuzaki K, Kim ... alpha-helices J Mol Biol 284, 867–873 Lau SY, Taneja AK & Hodges RS (1984) Synthesis of a model protein of defined secondary and quaternary structure Effect of chain length on the stabilization...

Ngày tải lên: 23/03/2014, 10:21

... lane 2, protein standards – phosphorylase a (98 kDa), BSA (67 kDa), ovalbumin (45 kDa), a-chymotrypsin (25 kDa) had a specific activity of 63 U:mg21 (Table 1) In SDS/ PAGE analysis the protein ... Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227, 680–685 32 Lowry, O.H., Rosenbrough, N.J., Farr, A.L & Randall, R.J (1951) Protein measurements with ... preparation was loaded on a DEAE-Sephadex column (50 Â 200 mm) equilibrated with buffer A and bound protein was eluted with M NaCl in the same buffer Following concentration to 25 mL on an Amicon...

Ngày tải lên: 24/03/2014, 04:21

Ngày tải lên: 30/03/2014, 09:20

... 3H-labelled UTP (0.5 lL of 50 CiÆmmol)1, mCiÆmL)1) and different concentrations of antibiotics Protein biosynthesis was assayed by incubating 10 lL of plasmolyzed cells in a total volume of 50 ... solution of trichloroacetic acid in water was added and the mixtures were incubated at °C for 30 (for protein biosynthesis assays, after the addition of trichloroacetic acid, samples were preincubated ... few minutes, in the same manner as the rifampicin control, while no effect is observed on DNA or protein synthesis Thus, the specificity of action observed with purified enzymes (Table 1) is confirmed...

Ngày tải lên: 30/03/2014, 15:20

... designed for the docking of small to medium sized organic molecules into protein binding sites [36] During the docking procedure, the protein is considered as rigid, whereas the ligand conformation is ... resulting from the response of the protein to the QA-reduction Hence, these absorption bands are indicative for the interaction between the quinone and the protein binding pocket Therefore, structural ... identical leading to the same protein environment formed predominantly by the side chains of HisM219, IleM223, MetM262 and IleM265 (Table 3) We have constructed hypothetical protein- ligand complexes...

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 01/04/2014, 10:08

... 2.1.7 Strong electrophiles, alkalis, acids, oxidants, or reductants that destroy tissue, DNA, or proteins 18 2.2 How to measure toxicity 18 2.2.1 Endpoints 18 2.2.1.1 Endpoints ... convince me that the fly has increased the nutritional value of the soup by adding vitamins and proteins, I will not have the soup Our comprehension of food quality and a pleasant environment ... and Klaassen (2001) 2.1.1 Enzyme inhibitors The toxicant may react with an enzyme or a transport protein and inhibit its normal function Enzymes may be inhibited by a compound that has a similar,...

Ngày tải lên: 03/04/2014, 12:11