prospects for high throughput structure determination by x ray crystallography
Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
Ngày tải lên :
07/03/2014, 17:20
... established by way of peroxidase activity assays and NMR spectroscopy Results Structure determination and overall structure of the various ferricyt c-550 models For the M100K variant two X- ray datasets ... group An X- ray dataset was collected for the wt protein ˚ from a single crystal at room temperature to 1.90 A resolution Structure determination by molecular replacement was straightforward by using ... As part of the present work the structure of cyt c-550 from P versutus has been determined by X- ray crystallography, allowing for a comparison in terms of overall structure and heme environment...
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Báo cáo Y học: Determinants of the inhibition of a Taiwan habu venom metalloproteinase by its endogenous inhibitors revealed by X-ray crystallography and synthetic inhibitor analogues pdf
Ngày tải lên :
08/03/2014, 23:20
... are grouped in a superfamily of metzincin which exhibits some typical structural features, such as the Met-turn and active-site consensus HExxHxxGxxH sequence [15–17] Some organisms and mammalian ... (1998) Crystallography & NMR system: a new software suite for macromolecular structure determination Acta Cryst D 54, 905–921 41 Engh, R.A & Huber, R (1991) Accurate bond and angle parameters for X- ray ... Tschesche, H., Huber, R & Bode, W (1995) X- ray structures of human neutrophil collagenase complexed with peptide hydroxamate and peptide thiol inhibitors Implications for substrate binding and rational...
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Báo cáo khoa học: Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf
Ngày tải lên :
23/03/2014, 07:20
... used for sulfate structures [open for sulfate_1* (see Experimental procedures), filled for sulfate_3], green triangles for PAH complexes (open for PAH_1, filled for PAH_2), a magenta square for ... favoured only for the former ligands for reasons that remain unclear (see above) The results for the single Mg trajectory (obtained by removing PEP and the site II metal from the PEP complex structure) ... different After exploring the neighbourhood of the starting conformation for approximately ns, there is a transition (Fig 3B) and the protein explores an area of much higher values for both EV1 (centred...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production ... particularly attractive for high- throughput production of proteins and selective isotope labelling starting from relatively expensive isotope labelled amino acids A straightforward combinatorial ... particular promise for accelerated studies of proteins by NMR spectroscopy by assigning residue type information to every amide cross-peak observed in [15N]-HSQC spectra We anticipate that high yield...
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Báo cáo lâm nghiệp: "Relationships between the intra-ring wood density assessed by X-ray densitometry and optical anatomical measurements in conifers. Consequences for the cell wall apparent density determination" pps
Ngày tải lên :
08/08/2014, 01:22
... lumen models Let X = · x = width of the double cell wall: s = R · T – [(R – X) · (T – X) ], s = X · (T + R) – X2 , s / S = cell wall proportion If drx = the wood density measured by x ray: apparent ... apparent density of the cell wall = drx / cell wall proportion = drx · S/s = drx · R · T / [X · (T + R) – X2 ] = drx · R · T / [X · (T + R – X) ] 2.3.2 Hexagonal model ST = area of measurement ... again, if drx = the wood density measured by x ray: apparent density of the cell wall = drx / cell wall proportion = drx · S/s = drx · H2 / (H2 – H’2) The use of rectangular and hexagonal models...
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![bioinformatics for high throughput sequencing [electronic resource]](https://media.store123doc.com/images/document/14/y/gu/medium_Yb8L87hXir.jpg)
bioinformatics for high throughput sequencing [electronic resource]
Ngày tải lên :
29/05/2014, 14:33
... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors ... molecular events by imaging Featured sequencing technologies include: GS FLX by 454 Life Technologies/ Roche, Genome Analyzer by Solexa/Illumina, SOLiD by Applied Biosystems, CGA Platform by Complete ... MG-RAST, a high- throughput system built to provide high- performance computing to researchers interested in analysing metagenomic data 1.5 What is Next? The increasing range of high- throughput...
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Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
Ngày tải lên :
18/06/2014, 18:20
... positive X ∗ and F (X ∗ ) We write • avoids expensive numerical approximation of steady states and can be scaled to highdimensional models X= X J∗ = G (X ∗ ) L (X ∗ ) gX − X X X Also in larger models ... log G ∂ log L where gX := ∂ log X |X= X ∗ and X := ∂ log X |X= X ∗ are so-called elasticities, a term mainly used in eco∗ ∗ nomics The prefactors G (X ) and L (X ) denote perX∗ X capita gain and loss ... of X ∗ one can use dynamical systems theory and evaluate the Jacobian of (1) at X ∂G ∂L J∗ = − X X =X X X =X Conclusion The simple example already shows that generalized modeling For expressing...
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Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
Ngày tải lên :
20/06/2014, 01:20
... positive X ∗ and F (X ∗ ) We write • avoids expensive numerical approximation of steady states and can be scaled to highdimensional models X= X J∗ = G (X ∗ ) L (X ∗ ) gX − X X X Also in larger models ... log G ∂ log L where gX := ∂ log X |X= X ∗ and X := ∂ log X |X= X ∗ are so-called elasticities, a term mainly used in eco∗ ∗ nomics The prefactors G (X ) and L (X ) denote perX∗ X capita gain and loss ... of X ∗ one can use dynamical systems theory and evaluate the Jacobian of (1) at X ∂G ∂L J∗ = − X X =X X X =X Conclusion The simple example already shows that generalized modeling For expressing...
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Báo cáo toán học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" ppt
Ngày tải lên :
20/06/2014, 21:20
... positive X ∗ and F (X ∗ ) We write • avoids expensive numerical approximation of steady states and can be scaled to highdimensional models X= X J∗ = G (X ∗ ) L (X ∗ ) gX − X X X Also in larger models ... log G ∂ log L where gX := ∂ log X |X= X ∗ and X := ∂ log X |X= X ∗ are so-called elasticities, a term mainly used in eco∗ ∗ nomics The prefactors G (X ) and L (X ) denote perX∗ X capita gain and loss ... of X ∗ one can use dynamical systems theory and evaluate the Jacobian of (1) at X ∂G ∂L J∗ = − X X =X X X =X Conclusion The simple example already shows that generalized modeling For expressing...
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Báo cáo hóa học: " Determination of InN/Diamond Heterojunction Band Offset by X-ray Photoelectron Spectroscopy" docx
Ngày tải lên :
21/06/2014, 11:20
... effect measurement in the InN/sapphire film The XPS measurements were performed on a PHI Quantera SXM instrument with Al Ka (energy 1486.6 eV) as the X- ray radiation source, which had been carefully ... VBM stands for valance band maximum The In3d5/2 spectra for the InN and InN/diamond samples, the C1s spectra for the diamond and InN/diamond samples, and the valence band photoemission for both ... http://www.nanoscalereslett.com/content/6/1/50 Page of Table XPS CL spectra fitting results and VBM positions obtained by linear extrapolation of the leading edge to the extended base line of the VB spectra Sample...
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Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt
Ngày tải lên :
09/08/2014, 22:23
... LPC X Cer CL 10 X LCL 2 XX DAG 13 PE 22 XX PE-O LPE 35 X X PG 10 XX PI 13 XX PS 10 XX SM TAG 25 a X X X XX X X X X X X X X X X X X X MFQL queries identifying species of these lipid classes are presented ... LipidXplorer Lipid classa Number of identified species Number of signature ions FAb FAOb HGb NLb MSc X X d X X PC PC-O 13 17 4 XX X LPC X Cer CL 10 X LCL 2 XX DAG 13 PE 22 XX PE-O LPE 35 X X PG ... Therefore, we next tested if the alignment accuracy was affected by the complexity of the analyzed lipid mixtures and by chemical noise To this end, we compared lipid species identified by LipidXplorer...
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Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes" pdf
Ngày tải lên :
09/08/2014, 22:24
... nucleotide file It performs automatic IS-associated ORF identification using IS-associated transposase and transpositionrelated (for example, regulatory) gene models (provided by ISfinder) for ‘.fasta’ ... will evolve with time and experience Exploitation of ISsaga Genome context One useful feature of ISsaga is that it supplies the genome context (that is, flanking genes) for each annotated Page of ... such studies in two ways: firstly by enriching the ISfinder database by high throughput annotation of completely assembled and scaffold-based genomes; and secondly by direct analysis of the metagenomes...
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báo cáo khoa học: " HAPIscreen, a method for high-throughput aptamer identification" pptx
Ngày tải lên :
11/08/2014, 00:23
... SELEX, each against target premiRs, constituting mixtures M1 and M2, were performed on the automated workstation For each SELEX, μM of the RNA library was heated at 80°C for min, cooled at 4°C for ... Bordeaux, France 3Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France Authors’ contributions ED carried out, designed and analyzed the SELEX experiments ST designed and performed ... [35,36] SELEX was performed against two mixtures M1 and M2 of three human premiRs each (a, b, c and x, y, z, respectively) as described in Materials and Methods At the end of seven SELEX rounds,...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
Ngày tải lên :
13/08/2014, 13:20
... response by itself, it can be considered as a highly specific CXCR4 antagonist [26-28] It was demonstrated that two aspartate residues at positions 171 and 262 of CXCR4 are crucial for the high- affinity ... used for the antiviral evaluation of CCR5 or CXCR4 antagonists As shown in Figure 1, all three cell lines highly expressed the primary HIV-1 receptor CD4 In addition, U87.CD4.CCR5.CXCR4 cells expressed ... easily infectable by X4 strains but, unexpectedly, not by R5 strains CCR5- or CXCR4-transfected U87.CD4 cells are also widely used, and are even Page of 13 (page number not for citation purposes)...
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Báo cáo y học: "A method for high-throughput gene expression signature analysis" ppt
Ngày tải lên :
14/08/2014, 16:21
... assay for highthroughput gene expression profiling on universal array matrices Genome Res 2004, 14:878-885 Gene Expression Omnibus [http://www.ncbi.nlm.nih.gov/geo/] NetAffx [http://www.affymetrix.com/analysis/index.affx] ... spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes Wells were emptied by centrifugation, as before A volume of 15 μl of a HotStarTaq DNA Polymerase mix (Qiagen, ... measure- We next considered the extent to which LMF could recapitulate the gene expression signature discovered with microarrays Estimates of the extent of differential expression reported by both...
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Structure of hibiscus latent singapore virus determined by x ray fiber diffraction
Ngày tải lên :
10/09/2015, 15:53
... solved by X- ray FD Surprisingly, the TMV CP structure was solved at 2.4 Å resolution by X- ray crystallography method (Bhyravbhatla et al., 1998) But for other Tobamovirus structures X- ray crsyatllography ... Bian for providing the X- ray fiber package for data reduction and structure refinement I also express my thanks to Dr Toshiro Oda for his support and technical expertise in solving the virus structure ... Figure 4.14 Stereo view of HLSV axial intersubunit carboxyl-carboxylate interactions at high radius region x 62 Figure 4.15 Stereo diagram of HLSV carboxyl-carboxylate interactions at the low radius...
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Development and application of advanced proteomic techniques for high throughput identification of proteins
Ngày tải lên :
16/09/2015, 08:30
... Y., Uttamchandani, M & Yao, S.Q Microarray: a versatile platform for highthroughput functional proteomics Comb Chem High Throughput Screen 9, 203-212 (2006) x List of Tables Table 2.1 Proteins related ... spotted with high density on the array, information of the whole genome of an organism can be virtually procured in a single experiment, making it an ideal tool for high- throughput exploration ... library, followed by facile identification of the enzyme-encoding genes with the decoding DNA microarray We envision that Expression Display will be potentially applicable for high- throughput characterization...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
Ngày tải lên :
07/03/2014, 17:20
... statistics for the CTXS-Xyl5-Me complex are summarized in Table Atomic co-ordinates for the crystal structures of TRX I, TRX II, CTX, and CTXS-Xyl5-Me complex can be found Table X- ray diffraction ... CTXS-Xyl2-Me complex were not detected within [S-Xyl2-Me]initial ẳ 5200 lM) Protein Inhibitor Ka ã 10)3 (M)1) TRX I S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me ... decreased in the series b-d-xylohexaose (Xyl6) > b-d-xylopentaose (Xyl5) > b-d-xylotetraose (Xyl4) > b-d-xylotriose (Xyl3), with no detectable afnity for b-d-xylobiose (Xyl2), which is consistent...
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