... cleft, the putative catalytic zinc being at the bottom of the cleft and a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8]. Grad- ual depletion of the ... Novagen. Expression of Pt-gdh under the control of araB promoter was induced for 4 h at 30 °C by the addition of 0.2% ara- binose when the A 600 of the growing culture reached 0.5. The cells from a 1-L ... tested, i.e. from 0.05 to 1 mm. The influence of Zn 2+ on the pH stability of GdhA is most evident after incubation (1 h, 55 °C) at pH 3.5, where, in the presence of the metal ion at 0.1 mm, there was...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster ppt
Ngày tải lên: 29/03/2014, 08:20
Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... c, with contributions from the formyl groups and from the geranyl side chain expected from heme a and a 3 . In addition to the signals of the hemes, the reorgan- ization of the polypeptide backbone ... and on this basis the contributions of the reorganization of the polypeptide backbone, of individual amino acids and of the hemes c, a and a 3 upon electron transfer to /from the redox active centers ... particular problem of the assignment in the difference spectra is the superposition of signals from different constituents of the oxidase, which can lead to the possibility of multi component...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... of the structure of NADP + -bound FeR revealed that the nicotinamide moiety of NADP + faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... direction along the b10 of the other mono- mer, forming an anti-parallel intermolecular b sheet (Fig. 2A). The subsequent residues of the loop (Ala7– Pro13) turn into the interior of the dimer interface, and ... fulgidus FeR is one of the candidates for the ferric ion-binding residue based on the structure of the mercury-bound derivative, although a ferric ion was not found at the site of either the native or...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo Y học: The membrane-bound [NiFe]-hydrogenase (Ech) from Methanosarcina barkeri : unusual properties of the iron-sulphur clusters docx
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
Ngày tải lên: 23/03/2014, 15:20
Thermal properties of the vernacular buildings envelopes: the case of the "Sassi di Matera" and "Trulli di Alberobello
Ngày tải lên: 05/09/2013, 14:58
Tài liệu Psychometric properties of the quality of life scale Child Health and Illness Profile-Child Edition in a combined analysis of five atomoxetine trials pdf
... allowing the assessment of the patient’s QoL both from the parent’s and from the patient’s perspective. The possibility to assess QoL from different perspectives is a promising character- istic of ... mainly consists of items from the sub- domains IRA and TA, which together form the Risk avoid- ance domain. High loadings of the second factor came almost exclusively from the EC domain. The third ... 10.1007/s12402-011-0066-y also for the change from baseline. The same was found to be true for the Comfort domain regarding the correlation of the domain with the total score. The Achievement domain, the Satisfaction...
Ngày tải lên: 12/02/2014, 19:20
Tài liệu Actions Against Abuse of the Global Financial System: Report from G7 Finance Ministers to the Heads of State and Government docx
... ongoing initiatives in the FATF and the OECD, the Report of the OFCs Working Group of the Financial Stability Forum (FSF) has made recommendations to enhance OFCs’ observance of international standards ... characterized by the high mobility of funds and the rapid development of new payment tools. To secure the benefits of the international financial system, we, the Finance Ministers of the G-7 countries, ... programs and the integrity of the IFIs themselves. Thus, we urge the IMF and the World Bank to continue to conduct an authoritative review of their financial procedures and controls and those of recipients,...
Ngày tải lên: 17/02/2014, 21:20
Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the (s i , ... 2009 The Authors Journal compilation ª 2009 FEBS parameters and, for clarity of exposition, we present the analysis of the Src cycle in terms of Eqns (6–8). Intrinsic regulatory properties of the ... steady states of the Src cycle. When there are three intersections, the steady state O 1 at the lower branch of the Z-shaped curve (i.e. low Src activity) and the state O 3 at the upper branch...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro- teins. The ... comparable with the two-metal-ion mechanism of both RNA and DNA synthesis [17]. In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plas- mid pIT3 isolated from ... is located at the bottom of the Zn-stem). However, a MD simulation on the Rep245 model showed that the absence of the two disulfide bridges did not affect the overall protein fold. The Zn-binding...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium doc
... transfer from CDH to cytochrome c, Phe166, which is located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the ... spectra of heme in CDH (A) and cytochrome c (B) for comparison of the isosbestic points. The spectra of the oxi- dized (solid line) and reduced (dashed line) forms are compared in the range of 450–650 ... This was expected from the thermodynamic result that the redox potential of heme in F166Y was lower than that of WT. However, when the electron transfer rate k (s )1 ) and the driving force DG...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... years ago and supports the notion of a universal occurrence of hemocyanins in the Myriapoda [15,16]. At least three hemocyanin subunits were present at the time of divergence of the Chilopoda and ... the determination of the N-terminal sequences, C. Hunzinger for the MALDI-TOF analyses, C. Bache and J. Hermanns for their help with the cloning experiments, and J. R. Harris for correcting the ... kDa, the isoelectric points (pI) range from 5 to 6.5 (Fig. 2). MALDI-TOF analyses of tryptic peptides show that the spots represent four distinct subunit types, as deduced from the pattern of identical...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Out of the Shadows African American Baseball from the Cuban Giants to Jackie Robinson ppt
... was good, for the owners both of the Negro team and the local team. The attendance for the annual game was often the highest of the year. ✶ Parallel with serious Negro ball were the black clown ... Introduction Jerry Malloy 1 The Birth of the Cuban Giants: The Origins of Black Professional Baseball Lee Lowenfish 15 When All Heaven Rejoiced: Branch Rickey and the Origins of the Breaking of the Color Line ... that imitated the standards of the white community that rejected them, while maintaining the unique African American heritage that was the root cause of that exclusion. In the birth of the Cuban...
Ngày tải lên: 21/02/2014, 06:20
Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... ielded from solution by the rest of the protein. There is no correlation between the complex stability and the rate of its formation. The Ni(II) for Cu(II) exchange rates f or peptides are o f the ... the rapid formation of the Cu(II) species, and there is little assistance from the buffer components. There is no accel- eration for DAHKam, compared to DAHam, in accord- ance with the lack of ... albumins. On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained. These data can be very useful in further studies of the toxicolo- gically...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... inserted between the third letter of one codon and the first letter of the following codon. Molecular modeling of the C. sepium b-amylase The amino-acid sequence of the b-amylase from C. sepium exhibits ... built for the b-amylase from C. sepium using the X-ray coordinates of the soybean b-amylase (Fig. 4). According to the Ramachandran plot of this model the f and c angles of most of the residues ... sequence of the protein is underlined. The arrowheads indicate the positions of the intron sequences. (B) Comparison of the amino-acid sequences of b-amylase from C. sepium (this work) with those from...
Ngày tải lên: 22/02/2014, 07:20
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