... cleft, the putative catalytic zinc being at thebottomofthe cleftand a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8]. Grad-ual depletion ofthe ... Novagen.Expression of Pt-gdh under the control of araB promoterwas induced for 4 h at 30 °C by the addition of 0.2% ara-binose when the A600 of the growing culture reached 0.5. The cells from a 1-L ... tested, i.e. from 0.05 to 1 mm. The influence of Zn2+on the pH stability of GdhA is most evidentafter incubation (1 h, 55 °C) at pH 3.5, where, in the presence ofthe metal ion at 0.1 mm, there was...
... c, with contributions fromthe formyl groupsand fromthe geranyl side chain expected from heme a anda3. In addition to the signals ofthe hemes, the reorgan-ization ofthe polypeptide backbone ... andon this basis the contributions ofthe reorganization of the polypeptide backbone, of individual amino acids and of the hemes c, a and a3upon electron transfer to /from the redoxactive centers ... particular problem ofthe assignment in the differencespectra is the superposition of signals from differentconstituents ofthe oxidase, which can lead to the possibility of multi component...
... ofthe structure of NADP+-bound FeRrevealed that the nicotinamide moiety of NADP+faces the re-side ofthe isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... direction along the b10 ofthe other mono-mer, forming an anti-parallel intermolecular b sheet(Fig. 2A). The subsequent residues ofthe loop (Ala7–Pro13) turn into the interior ofthe dimer interface,and ... fulgidusFeR is one ofthe candidates for the ferric ion-bindingresidue based on the structure ofthe mercury-boundderivative, although a ferric ion was not found at the site of either the native or...
... allowing the assessment ofthe patient’s QoL both fromthe parent’sand fromthe patient’s perspective. The possibility to assessQoL from different perspectives is a promising character-istic of ... mainly consists of items fromthe sub-domains IRA and TA, which together form the Risk avoid-ance domain. High loadings ofthe second factor came almostexclusively fromthe EC domain. The third ... 10.1007/s12402-011-0066-yalso for the change from baseline. The same was found tobe true for the Comfort domain regarding the correlation of the domain with the total score. The Achievement domain, the Satisfaction...
... ongoing initiatives in the FATF and the OECD, the Report ofthe OFCs Working Group ofthe Financial Stability Forum (FSF) has made recommendations to enhance OFCs’ observance of international standards ... characterized by the high mobility of funds and the rapid development of new payment tools. To secure the benefits ofthe international financial system, we, the Finance Ministers ofthe G-7 countries, ... programs and the integrity ofthe IFIs themselves. Thus, we urge the IMF and the World Bank to continue to conduct an authoritative review of their financial procedures and controls and those of recipients,...
... presenting the pulse of Src activity in the plane of the inactive and active fractions, siand s1. If the duration ofthe stimulus exceeds the critical value, the trajectory in the (si, ... 2009 The Authors Journal compilation ª 2009 FEBSparameters and, for clarity of exposition, we present the analysis ofthe Src cycle in terms of Eqns (6–8).Intrinsic regulatory propertiesofthe ... steady states ofthe Src cycle. When there are threeintersections, the steady state O1at the lower branch of the Z-shaped curve (i.e. low Src activity) and the stateO3at the upper branch...
... analyses ofthe predictedamino acid sequence showed that the C-terminal half of the RepA ofthe pIT3 plasmid is sequence-similar to the helicases ofthe phage-encoded superfamily III pro-teins. The ... comparablewith the two-metal-ion mechanism of both RNA andDNA synthesis [17].In a previous study, we reported the findings of ananalysis ofthe complete sequence ofthe cryptic plas-mid pIT3 isolated from ... is located at the bottomofthe Zn-stem). However, a MD simulationon the Rep245 model showed that the absence of the two disulfide bridges did not affect the overallprotein fold. The Zn-binding...
... transfer from CDH to cytochrome c, Phe166, which is located in the cytochromedomain and approaches one of propionates of heme, was mutated to Tyr,and the thermodynamic and kinetic propertiesofthe ... spectra of heme in CDH (A) and cytochrome c(B) for comparison ofthe isosbestic points. The spectra ofthe oxi-dized (solid line) and reduced (dashed line) forms are compared in the range of 450–650 ... Thiswas expected fromthe thermodynamic result that the redox potential of heme in F166Y was lower than that of WT. However, when the electron transfer rate k(s)1) and the driving force DG...
... years agoand supports the notion of a universal occurrence of hemocyanins in the Myriapoda [15,16]. At least threehemocyanin subunits were present at the time of divergence of the Chilopoda and ... the determination ofthe N-terminal sequences, C.Hunzinger for the MALDI-TOF analyses, C. Bache and J. Hermannsfor their help with the cloning experiments, and J. R. Harris forcorrecting the ... kDa, the isoelectricpoints (pI) range from 5 to 6.5 (Fig. 2). MALDI-TOFanalyses of tryptic peptides show that the spots representfour distinct subunit types, as deduced fromthe pattern of identical...
... was good, for the owners both of the Negro team and the local team. The attendance for the annual game wasoften the highest ofthe year.✶Parallel with serious Negro ball were the black clown ... IntroductionJerry Malloy 1 The Birth ofthe Cuban Giants: The Origins of Black Professional BaseballLee Lowenfish 15 When All Heaven Rejoiced: BranchRickey and the Origins ofthe Breaking of the Color Line ... that imitated the standards ofthe whitecommunity that rejected them, while maintaining the unique AfricanAmerican heritage that was the root cause of that exclusion. In the birth of the Cuban...
... ielded from solution by the rest ofthe protein. There is no correlation between the complex stability and the rate of its formation. The Ni(II) for Cu(II) exchange rates f or peptides are o f the ... the rapid formation ofthe Cu(II) species, and there is littleassistance fromthe buffer components. There is no accel-eration for DAHKam, compared to DAHam, in accord-ance with the lack of ... albumins. On the other hand, the directthermodynamic and kinetic characterization of Ni(II)binding at site I in HSA and BSA was obtained. Thesedata can be very useful in further studies ofthe toxicolo-gically...
... inserted between the third letter of one codon and the first letter ofthe following codon.Molecular modeling of the C. sepiumb-amylase The amino-acid sequence ofthe b-amylase from C. sepiumexhibits ... built for the b-amylase from C. sepium using the X-ray coordinates ofthe soybeanb-amylase (Fig. 4). According to the Ramachandran plot of this model the f and c angles of most ofthe residues ... sequence of the protein is underlined. The arrowheads indicate the positions ofthe intron sequences. (B) Comparison of the amino-acid sequences of b-amylase from C. sepium (this work) with those from...