properties of dna and native nucleic acid based nanostructures
DETERMINATION OF DNA AND OF OTHER NUCLEIC ACID FRACTIONS
... determination of dna in Saccharomyces cerevisiae with 10 ml of water (10 min) (C) The sample was extracted for IS with ml of a solution containing 0-4 mol of perchloric acid -(- 4-0 mol of acetic acid/ litre, ... preparatory to analysis for nucleic acids Vol 86, 1980] 173 trevelyan: determination of dna in Saccharomyces cerevisiae TABLE II Content of Total, Nucleic Acid, and Nucleolide Purinc in Baker's ... Journal of the Institute of Brewing, 1966, 52, 184 17 Trcvelyan, W E., Journal of the Science of Food and Agricui~ lure, 1975, 26, 1673 18 Trevelyan, W E., Journal of the Science of Food and Agricullure,...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... presence and absence of DNA (A) Effect of pressure on the dlFG coat protein at room temperature was analyzed in the absence of nucleic acids (m) and in the presence of 34-bp DNA poly(GC) (.) and yeast ... presence of both DNA and bis-ANS decreased the stability of the protein (Fig 7B) Effects of high temperature on the secondary and tertiary structure of dlFG and sc-dlFG in the absence and presence of ... nm) Nucleic acid- binding assays The effect of the presence of nucleic acids on the stability of dlFG was analyzed during pressurization The protein was incubated with poly(GC) DNA, yeast tRNA and...
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Báo cáo hóa học: " Initial susceptibility and viscosity properties of low concentration e-Fe3 N based magnetic fluid" pdf
... holder is in the shape of a cylinder and a ratio between the height and diameter equal to Due to the low concentration of the particle in the samples, and the high aspect ratio of the cylinder, the ... some amount of large particles, and the shape of which differs essentially from spherical The shape anisotropy of non-spherical particles will hinder the free rotation of the particles and therefore ... size of dTEM = 14 ± nm Volume fraction of solids Magnetic measurement The concentration of the MF samples is determined as following method First we measure the mass M of a certain volume VF of...
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Synthesis and Physical Properties of Polyester Amides Derived from Lipid-Based Components
... α-amino acids, diols and fatty dicarboxylic acids 124,164 80 to82 240 to 250 6.7 to 32.8 Copolymer Random combination of polyester and nylon BAK 1095 Caprolactam (Nylon 6) Butanediol, Adipic acid ... (5%) 155 Features Derived from oleic acid Linseed oil, diethanolamine DEA Anticorrosive coatings and diacid From fatty acids Pongamia glabra oil, DEA and diacid 275 (10%) Anticorrosive coatings ... applications and adipic acid Gallic acid, amino acid 249-305 (5%) 141-168 180 Highly branched, aromatic 120 Potental antibacterial activities Castor/soyabean oil, DEA, adipic acid and 260 Zn(OH)2...
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DESIGN, SYNTHESIS AND STUDY OF DNA-TARGETED BENZIMIDAZOLE-AMINO ACID CONJUGATES
... the elucidation of the structure of DNA and an understanding of the role of DNA in the life cycle of a cell The interaction of small molecules with DNA is also a prolific area of study because ... Dogma of Molecular Biology” 1.2 Primary structure of DNA 1.3 A•T and G•C Watson-Crick base pairs of DNA 1.4 Structures of A, B, and Z -DNA 1.5 Structure of the ... to understand the structure of DNA itself Figure 1.1 The “Central Dogma of Molecular Biology.” 1.2.2 Structure of B-Form DNA DNA is composed of two phosphodiester-linked nucleotide strands that...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... combination of vectors: pcDNA3, pcDNA3 ⁄ IL-1+ARE, pcDNA3 ⁄ IL-1)ARE, pcDNA3 ⁄ MCPIP, MCPIP ⁄ IL-1+ARE, MCPIP ⁄ IL-1)ARE and pcDNA3 ⁄ MCPIP The pcDNA3 vector served as a control for the experiment, and ... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands)...
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Tài liệu PHYSICAL, CHEMICAL, AND MECHANICAL PROPERTIES OF BAMBOO AND ITS UTILIZATION POTENTIAL FOR FIBERBOARD MANUFACTURING docx
... utilization The physical and mechanical properties of bamboo vary with the age of the bamboo and the height of the culm [Chauhan 2000] In general, SG and the properties of bamboo drop from the ... characteristics and mechanical properties of bamboo Dissertation, Chinese Academy of Forestry, Beijing, China pp 147 Yusoff, M.N.M, A Abd.Kadir, and A.H Mohamed 1992 Utilization of bamboo for pulp and paper ... on the utilization of bamboo fibers to MDF This chapter investigated the effects of age of bamboo fibers and the resin content level on the physical and mechanical properties of the manufactured...
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Báo cáo khoa học: Human telomeric G-quadruplex: structures of DNA and RNA sequences ppt
... G-tracts of one strand and only the 3¢-end G-tract of the other strand [30] (Fig 3D) The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one strand ... conformations of guanines along two consecutive G-tracts are 5¢-syn-anti-anti-3¢ and 5¢-syn-syn-anti-3¢ for one strand and 5¢-syn-syn-anti-3¢ and 5¢-syn-anti-anti-3¢ for the other strand of the dimer; ... DNA A Long human telomeric DNA and RNA sequences The next step toward understanding the structure of the ‘real’ telomeres is to address the question on the structure of long human telomeric DNA...
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GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH pptx
... on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB ... reverse 2-hybrid based selection of random mutations (described below), and biochemical characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork ... translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis We constructed the DNA substrate shown in Fig The DnaB helicase enters the substrate from the left by riding the 5’-single-stranded...
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Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx
... models for Arenicola TK and MiTK were constructed, based on the structures of chicken and human MiCKs Alignment of amino acid sequences of phosphagen kinases and construction of phylogenetic tree ... mitochondrial isoform (referred to henceforth as MiTK) as described below The cDNA for the cytoplasmic TK consists of 1811 bp with an ORF of 1098 bp and 5¢ and 3¢ untranslated regions of 21 and 692 bp, ... AB186411 The cDNA for the MiTK consists of 1680 bp with an ORF of 1239 bp and 5¢ and 3¢ untranslated regions of 68 bp and 373 bp, respectively The ORF codes for a protein containing 412 amino acid residues...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... lg of platelet protein and 0.5 lg of GST and GST±Rab proteins; (B) an [a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lg of GST and 0.5 lg of ... form of Rab32 (see Discussion) Fig Puri®cation and properties of wild-type and mutant Rab31 and Rab32 expressed as GST±fusion proteins Samples of platelet particulate fraction protein and of puri®ed ... the latter differed by two nucleotides and one amino -acid residue from that Fig Alignment of the deduced amino -acid sequences of Rab31 and Rab32 with those of closely related Rab proteins Multiple...
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Competitive sorption of cadmium and lead in acid soils of central spain
... accounting for the 52% and 69% of the ECEC, respectively Soils S1 and S3 had the lowest ECEC, pHw and clay content while S2 and S4 were less acidic and showed higher ECEC and clay content Soil ... kaolinite and more smectite and illite Both S1 and S2 had similar proportions of phyllosilicates in the V mm soil fraction and of goethite and haematite in the clay fraction The greater content of illite ... intervals of the estimated exponents, and the standard error of the estimate are shown in Table The average value of the exponents found for S1, S2 and S3, were 0.03 F 0.015 (standard deviation) and...
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Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx
... both in shape and in dimension to the fibrils of hTTR and sbTTR formed by heating The thick and straight morphology of heat-induced fibrils of hTTR, sbTTR and ecTRP is similar to that of amyloid fibrils ... of hTTR, sbTTR, and ecTRP Results Partial acid denaturation generates fibrils of hTTR and sbTTR Partial acid denaturation combined with turbidity assays is a method frequently used to induce and ... Stability and fibril formation of TTR and TRP Fig DSC profiles of hTTR, sbTTR, and ecTRP The melting temperatures (Tm) were 97.8 °C for hTTR, 93.0 °C for sbTTR, and 66.7 °C for ecTRP Fig Analysis of tetramer...
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First principles optical properties of silicon and germanium nanowires
... EMA models Optical properties of SiNWs and GeNWs In Section of the present paper, we aim to point out the importance of the many-body effects on the optical response of some of the studied nanowires ... in terms of the different geometrical structure of wires grown with different orientation Finally the comparison of the optical spectra of SiNWs and GeNWs with diameters of the order of 0.8 nm, ... Optical gaps in SiNWs and GeNWs: quantum confinement and anisotropy effects In this section, we will describe the electronic properties of hydrogen passivated, free standing silicon and germanium nanowires...
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Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx
... mixture of : of E and Z isomers of 4-hydroxy-8-sphingenine has been cloned and characterized [20] A single fatty acid desaturase enzyme has been implicated in both E and Z D11 desaturation of myristoyl-CoA ... 18:3D6Z,9Z,12Z and 18:2D6a,9Z,12Z accounted for about 1.3% of all fatty acids in the cell and of which 40% was found as the acetylenic fatty acid and thus had undergone two rounds of desaturation ... 3% of 18:2D9Z,12E and 80% of a-eleostearic acid (18:3D9Z, 11E,13E) and it is probable that the relative proportion of oleate and linoleate in these seeds also determines the relative rate of...
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The properties of gases and liquids, fifth edition poling, prausnitz, o’connell
... temperature and density The correlation of a and b in terms of other properties of a substance is an example of the use of an empirically modified theoretical form Empirical extension of theory can often ... scientists and engineers often require the properties of gases and liquids The chemical or process engineer, in particular, finds knowledge of physical properties of fluids essential to the design of many ... THE PROPERTIES OF GASES AND LIQUIDS CHAPTER ONE THE ESTIMATION OF PHYSICAL PROPERTIES 1-1 INTRODUCTION The structural engineer cannot design a bridge without knowing the properties of steel and...
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Genetic Manipulation of DNA and Protein – Examples from Current Research Edited by David Figurski potx
... on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB ... reverse 2-hybrid based selection of random mutations (described below), and biochemical characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork ... translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis We constructed the DNA substrate shown in Fig The DnaB helicase enters the substrate from the left by riding the 5’-single-stranded...
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sensitivity properties of a novel no2 gas sensor based on mesoporous wo3 thin film
... process of NO2 molecules because of the capillary pore and large surface area This implies the conclusion that this type of film will offer a good sensitivity to NO2 gas The morphology of the mesoporous ... 2.0E+005 ↑H2 off 0.0E+000 1000 2000 3000 Time (sec) Fig Dependence of electrical resistance of WO3 thin films upon operating temperatures of (a) 35, 50, 70 and 100 ◦ C for ppm NO2 gas and (b) 100 ... excellent sensing properties upon exposure to low concentration of NO2 in air at low temperatures and enabled the selective detection of NO2 and H2 gases A high surface area and small crystallites...
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structural and photocatalytic properties of iron- and europium-doped tio2
... that of TiO2 , suggesting similar Fe and Ti surroundings in the doped and undoped samples In order to verify this statement, the filtered EXAFS of L Diamandescu et al / Materials Chemistry and ... membrane filters and analysed on a DANI GC 1000 gas chromatograph Fig XRD patterns of hydrothermally synthesized doped and undoped TiO2 148 L Diamandescu et al / Materials Chemistry and Physics 112 ... L Diamandescu et al / Materials Chemistry and Physics 112 (2008) 146–153 Experimental Results and discussion 2.1 Hydrothermal synthesis 147 3.1 X-ray diffraction Fe3+ - and Eu3+ -doped and -codoped...
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mechanical properties of polymers and composites nielsen doc
... Effect of MokcLil.ir Weight Effect of Cross-linking Effects of Crystallinity and Morphology Effects of Plasticizcrs and Copolymerization Effect of Molecular Orientation Effect of Strength of Intcrmolccular ... dependence on temperature and time of the properties of polymers compared to those of other materials such as metals This strong dependence of properties on temperature and on how fast the ma terial ... specialized and have not been officially recognized as standardized tests Some of these tests, however, have been standardized and are described in the publications of the American Society for Testing and...
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