programmed cell death in fetal oocytes

... requires protein synthesis ANT1-silencing induces ADF cell apoptosis and a non-apoptotic cell death modality similar to paraptosis To gain more insight regarding the observed reduction in cell viability, ... to propidium iodide staining (data not shown), indicating that the plasma membrane is maintained intact, allowing us to exclude primary necrosis as the principal cell death modality Moreover, ... ANT1-silencing induces glioblastoma cell death A Lena et al Because of their multitask nature, ANTs are involved in several aspects of cell metabolism, as well as in cell survival ⁄ death processes,...

Ngày tải lên: 29/03/2014, 09:20

... Slovenia Jing Yin and Kevin S W Tan Staurosporine-induced programmed cell death in Blastocystis In 4th International Conference on Anaerobic Protists, 12 – 16 May 2008, Taoyuan, Taiwan Binhui Wu, Jing ... cell death in complete absence and independent of caspases in vitamin D-induced apoptosis of the breast cancer cell line MCF-7 (Mathiasen et al., 2002) 1.2.2 Type II cell death – autophagic cell ... Types of cell death .9 1.2.1 Type I cell death – apoptosis 10 1.2.2 Type II cell death – autophagic cell death 16 1.2.3 Type III cell death – necrosis 21 1.3 Programmed...

Ngày tải lên: 16/10/2015, 15:35

... actin cortex under the membrane of blebs in filamin-deficient blebbing cells has not been examined in blebs of cells undergoing cell death Although the proteins involved in the execution of blebbing ... tropomyosin and the actin-bundling protein, a-actinin Finally, myosin is recruited and is concentrated in a few distinct dots along the cortex [4] When examined by scanning electron microscopy in detergent-extracted ... can induce apoptosis by suppressing integrin-mediated cell adhesion and survival signalling [53], and can inhibit the association of talin head domain with integrin to suppress the integrin–Cdc42...

Ngày tải lên: 29/03/2014, 08:20

... 10.000 cells was analyzed Immunoblots Cells were collected and lysed in TNE buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% v/v NP40, mM EDTA) containing 10 μg/ml pepstatin/aprotinin/leupeptin, mM ... expressed in distinct cellular compartments The carboxyterminus of wildtype Bcl-2 contains a stretch of hydrophobic amino acids (the "insertion sequence") that has been proposed to anchor proteins in ... Zhao L, Wang X: Receptor interacting protein kinase-3 determines cellular necrotic response to TNF-alpha Cell 2009, 137:1100-1111 Zhang DW, Shao J, Lin J, Zhang N, Lu BJ, Lin SC, Dong MQ, Han J:...

Ngày tải lên: 09/08/2014, 10:20

... cell death in the lace plant [10], in induced cell death in lace plant protoplasts [26], and also during induced cell death by osmotic stress in tobacco suspension cultures [33] Increases in transvacuolar ... implication in victorin binding and induced cell death Plant J 2002, 29:295-312 Yu XH, Perdue TD, Helmer YM, Jones AM: Mitochondrial involvement in tracheary element programmed cell death Cell death ... our findings implicate the mitochondria as playing a critical and early role in developmentally regulated PCD in the lace plant Background Programmed cell death in plants Programmed cell death...

Ngày tải lên: 11/08/2014, 11:21

... cells Cell culture The human alveolar epithelial cell line A549 was maintained in RPMI 1640 medium (Nipro, Osaka, Japan) containing 10% heat-inactivated fetal bovine serum (Biological Industries, ... replicated in flow-cytometry analyses of infected A549 cells (Fig 6a and 6b) 40 Figure growth of L pneumophila in A549 cells Intracellular Intracellular growth of L pneumophila in A549 cells A549 cells ... < 0.05 Results Intracellular growth and cytotoxicity of L pneumophila in A549 cells First, we verified the infection and intracellular growth of L pneumophila in A549 cells Intracellular growth...

Ngày tải lên: 12/08/2014, 15:21

... Group IL Interleukin JNK N-terminal Kinases K-rasKirsten Rat Sarcoma LDH Laktatdehydrogenase MAPK = ERK Mitogen-activated protein kinase MAP4K1 Mitogen-activated protein kinase kinase kinase kinase ... Polymerase Chain Reaction Programmed cell death Platelet-Derived Growth Factor Phosphoinositid-3-Kinasen Phorbol Myristate Acetate papillary Renal Cell Carcinoma Retinsọure-Rezeptor Renal Cell Carcinoma ... immunhistochemische Fọrbungen nachzuweisen sind, klinisch effektiv verwertbar 31 1.6 Das Programmed cell death Protein Das Programmed cell death 4, kurz Pdcd4, ist ein Protein, das wọhrend der Apoptose hoch...

Ngày tải lên: 19/11/2015, 14:18

... apoptotic cell death in response to various stimuli To investigate a potential involvement of MAPK in ionizing radiationinduced cell death, we employed specific chemical inhibitors of MAPK As shown in ... examine the kinetics of the apoptotic cell death induced by ionizing radiation in human cervical cancer cells, we treated HeLa cells with 10 Gy radiation, and analyzed induction of apoptotic cell ... apoptotic cell death by fluorescence activated cell sorting (FACS) analysis with Annexin V staining Figure 1A shows that there is a time-dependent increase in apoptotic cell death, reaching approximately...

Ngày tải lên: 07/03/2014, 05:20

... autophagic cell death is a separate form of programmed cell death that is distinctly different from apoptosis In keeping with this interpretation, intensive irradiation led to up to 30% cell death in ... undifferentiated cells in the developing retinal tissue Inhibition of protein synthesis induced in the immature retina various post-translational, mitochondria-dependent pathways of cell death In one pathway ... autophagic cell death upon treatment with tamoxifen More recent work showed that in apoptotic cell death induced by tyrphostin A25 in the human colon cancer cell line HT29/HI1, early stages of the death...

Ngày tải lên: 07/03/2014, 15:20

... partly stained (Fig 6G,g) Discussion After the onset of spinning, ASGs undergo cell death in response to 20E treatment in vitro, but they not so before spinning [6] Although such differences in responsiveness ... to remain with the floss This finding indicates that the GOD enzyme in the ASG inner cavity was released with the silk at the early spinning stage Because the onset of spinning on day is induced ... Nongenomic action of an insect steroid hormone in steroid-induced programmed cell death Mol Cell Endocrinol 263, 18–28 Kakei M, Iwami M & Sakurai S (2005) Death commitment in the anterior silk gland...

Ngày tải lên: 15/03/2014, 00:20

... or in combination with other BOPs, has been implicated in melanoma cell death, arising from MEK1 ⁄ inhibition, in several other studies [44–46] It is interesting to note that in all cases MEK inhibitor-induced ... relapse on imatinib therapy have point mutations in the BCR–ABL kinase domain, including the T315I gatekeeper mutation that impairs imatinib binding [58,59] Accordingly, new therapies are being tested ... importance in cytokine-mediated survival in murine haematopoetic progenitor cells [61] Expression of BCR–ABL in inter6056 leukin-3-dependent Baf-3 cells represses BIM and allows interleukin-3–independent...

Ngày tải lên: 16/03/2014, 00:20

... 4671 JA and cell death C Reinbothe et al prodomains from caspases Interestingly, the mycotoxin and sphingosine analogue fumonisin-B1 is a powerful inducer of singlet oxygen-dependent PCD in animals ... and defence protein synthesis in the early stage and to shut-down protein synthesis in the terminal stages preceding or correlating with cell death Implication of JA in cell death regulation ... be involved in the regulation of cell death in cet plants [93,94] JA action on mitochondria links oxidative damage to cell death Mitochondria play an active role in cell death regulation in animals...

Ngày tải lên: 16/03/2014, 02:20

... isoleucine), which renders p53 nonfunctional [18] The cell line was maintained in serial passages in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 10 lgÆmL)1 insulin, ... extensively induce cell death in the human breast cancer cell line L56Br-C1 This cell line carries a germ-line mutation (1806 C fi T) in the BRCA1 tumour Fig Propidium iodide-stained nuclei of L56Br-C1 cells ... L56Br-C1 cells, the DENSPM-induced increase in SSAT activity was not as extensive as in any of those two cell lines The depletion of the polyamine pools was however, similar in all three cell lines...

Ngày tải lên: 17/03/2014, 23:20

... our in vivo and in vitro studies was threefold: a) to demonstrate that Ruta + Ca3(PO4)2 combination therapy can eliminate intracranial cancer cells, by either inducing cell death or preventing ... result in the cancer cells becoming more sensitive to cell death than normal cells (23) The Ruta and Ca3(PO4)2 combination was capable of protecting normal B-lymphoid cells against H2O2-induced ... apoptotic machinery in spontaneously regressing swine melanoma and/or drug-induced cell death in cancer cells (8-13,24,25) Irrespective of the as-yet-unknown protective mechanism(s) operating in normal...

Ngày tải lên: 22/03/2014, 17:20

... glutamine, cysteine, and glycine It is the most abundant intracellular small molecule thiol present in mammalian cells and it serves as a potent intracellular antioxidant protecting cells from toxins ... various death domain-containing proteins, forming a complex comprising TRADD, TNF Receptor Associated Factor-2 (TRAF2), cellular inhibitor of apoptosis-1 (CIAP1), and the receptor-interacting protein-1 ... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible...

Ngày tải lên: 23/03/2014, 17:20

... dioctylphtalate on h-Bax-induced cell death kinetics WtB1 cells were grown in a commercial Yeast Nitrogen Base medium (Difco, h), or in a home-made minimal medium in the absence (j) or in the presence ... Maccarrone, M., Melino, G & Finazzi-Agro, A (2001) Lipoxygenases and their involvement in programmed cell death .Cell Death Differ 8, 776–784 18 Gu, J., Liu, Y., Wen, Y., Natarajan, R., Lanting, L & Nadler, ... dioctylphtalate, Bax-induced cell death kinetics was much slower than in Yeast Nitrogen Base medium (Fig 6) The addition of dioctylphtalate restored cell death kinetics comparable to experiments in Yeast...

Ngày tải lên: 23/03/2014, 21:20

... blocking PD-1/PD-1L interaction, speaking to the critical role of PD-1 in determining the fate of CD8+ T cells post-priming (summary of results in refs [42] and [48]) In this experimental setting, ... immunogenicity in primates (24) (25) (26) (27) (28) (29) (30) Inactivated viruses Inactivated rabies Inactivated influenza Increased neutralizing immunity in mice, cattle Increased neutralizing antibody ... mechanism explaining the effectiveness of DNA priming - heterologous boosting in achieving superior immunity in immune competent organisms Alternating DNA priming with heterologous boosting (viral...

Ngày tải lên: 18/06/2014, 16:20

... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining ... treated cells showed abnormal monopolar spindles, suggesting that the inhibition of Aurora kinase activity induced defects of mitotic spindle in VX-680 treated cells VX-680 suppresses cell growth ... concentration in NB4-R2 cells (Figure 2C), indicating ATRA failed to inhibit NB4-R2 cells growth VX-680 decreases pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied...

Ngày tải lên: 18/06/2014, 19:20

... to dopaminergic neuronal death by oxidizing dopamine to a reactive dopamine quinone [51], by increasing DNA oxidation [7], or through increased microglial activation leading to chronic inflammation ... of oxidative and inflammatory insults The positive feed back loop may continue until the additional neuronal death (~50% of the remaining of the initial death) combined with the initial loss exceed ... Serotec, Oxford, UK) Sections were incubated in 4% normal serum in PBS for 30 minutes After this blocking step, the sections were incubated overnight in PBS containing 0.025% Triton X-100, 1% normal...

Ngày tải lên: 19/06/2014, 22:20

... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining ... treated cells showed abnormal monopolar spindles, suggesting that the inhibition of Aurora kinase activity induced defects of mitotic spindle in VX-680 treated cells VX-680 suppresses cell growth ... concentration in NB4-R2 cells (Figure 2C), indicating ATRA failed to inhibit NB4-R2 cells growth VX-680 decreases pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied...

Ngày tải lên: 20/06/2014, 03:20