production of highly sialylated monoclonal antibodies

... and probed with a panel of anti-MUC16 monoclonal antibodies Arrows indicate migration of 250 kDa molecular weight marker and identity of antibody used is shown on the right of each blot We have ... CA125 in spent media of MOVCAR-2 cells We therefore tested a panel of ten anti-MUC16 antibodies that we recently generated using the novel ABL-MYC technology All of these antibodies were able ... weight of MUC16 requires the use of extensive molecular biological approaches to study the importance of this mucin in the pathogenesis of ovarian cancer In addition, a thorough study of MUC16...

Ngày tải lên: 20/06/2014, 07:20

... to 1,000 nm) region of the spectrum [20] The advantage of NIR dyes is that they enable reasonable tissue penetration of exciting and emitted lights, while the amount of autofluorescence is negligible ... saline [PBS] instead of human serum For analysis of stability, samples were diluted threefold in PBS prior to HPLC analysis Biodistribution For evaluation of the biodistribution of 89Zr-cetuximab/ ... for this assay, and did not alter upon coupling of of eq of dye HPLC analysis confirmed that there was no difference in the conjugation efficiency of IRDye800CW to cetuximab/ bevacizumab or 89...

Ngày tải lên: 20/06/2014, 21:20

... experimental models of arthritis, therefore representing potential therapeutic targets [1] A specific therapeutic approach is the direct targeting of CD4+ T cells by use of monoclonal antibodies (mAbs) ... sake of simplicity, we refer to the mAb RIB5/2 as accelerating (although this term is applicable only to the onset of AA) and the mAbs W3/25 and OX35 as ameliorating Affinity of the monoclonal antibodies ... binding sites of W3/25 and OX35 are situated in the CC region of domain D1 and the BC region of domain D2 of the CD4 molecule, respectively [23], we could roughly localize the epitope of RIB5/2 in...

Ngày tải lên: 09/08/2014, 03:24

Ngày tải lên: 11/09/2015, 09:56

... Western blot of 14C10 mAb binds to E protein of DV1 Figure 7.10 Western blot and native gel of 14C10 mAb against DIII of E protein of DV 138 139 xiii Figure 7.11 Graph of binding affinities of 14C10 ... Sequences of IgG genes extracted from EBV-infected B cells suggest reactivation of SHM 5.3 Upregulation of AID in EBV infected B cells 117 119 Chapter – Production of neutralizing recombinant human monoclonal ... Mechanism of 14C10 mAb 153 7.9 ADE profiles of 14C10 mAb 155 7.9.1 Development of ADE among the DV serotype 155 7.9.2 Development of ADE among the antibody subclasses 157 7.10 Protective capacity of...

Ngày tải lên: 11/09/2015, 09:56

... hundreds of thousands of cases of DHF each year and without appropriate treatment, mortality can be over 20 % About 95 % of cases occur in children under 15 years of age and at least 5% of these ... by components of the adaptive immune system in DV immunopathology The hallmark of pathogenesis of DHF/DSS is the loss of endothelial integrity, which is assumed to be the result of an abnormal ... secretion and production of other cytokines This complex interactive network of induction results in further increases in the levels of cytokines and other chemical mediators Cytokines often exhibit...

Ngày tải lên: 11/09/2015, 09:56

... syassa tolbonummi rof desu saw leg rehto eht dna )A( GBC htiw deniats saw leg eno ,ED-2 retfA EGAP-SDS %5.21 yb dewollof ,)mc ,7-4 Hp( pirts yrd GPI gnisu gnisucof cirtceleosi yb demrofrep saw ED-2 ... deifirup gI hsifkcor htiw syassa tolbonummi ni niahc thgil eht rof cificeps erew )6-7C9R ,1-4H11R( senolc dna niahc yvaeh eht rof cificeps erew )cte ,9-4B7R ,8-4B7R( senolc 91 ,sbAM eht fO senolc ... secnerefeR noitadnuoF gnireenignE & ecneicS aeroK eht fo margorP hcraeseR cisaB eht morf )0-24200 -000-1002-10R( tnarg a yb detroppus saw krow sihT stnemgdelwonkcA esu larutluciram rof seniccav dna...

Ngày tải lên: 07/08/2014, 18:21

... repeated injections of massive doses of antibodies and (iii) the restrictive costs of in vitro produced proteins certified for human use Moreover, injection of massive doses of mAbs results in ... bioavaibility of these therapeutic agents that are often detrimental to the efficacy of treatments It is, therefore, important to investigate whether in vivo production of therapeutic antibodies ... neurodegenerative disease upon production of a neutralizing mAb [10] thereby demonstrating the therapeutical interest of the approach Neutralizing anti-HIV and anti-HCV antibodies, some of which are currently...

Ngày tải lên: 14/08/2014, 19:22

... 38873898 ê 2005 FEBS S N Fedosov et al Mapping of transcobalamin using antibodies Fig Binding of the antibodies to CNBr cleavage peptides of TCy CNBr peptides of TCy were fractionated by HPLC and subjected ... and 3891 Mapping of transcobalamin using antibodies S N Fedosov et al Fig Binding of antibodies to the proteolytic fragments of human TC from yeast and plants (a) Fragments of TCy (Left) Coomassie ... absence of lm TCp11 + TCp12 [A320ETIP and TQEII RLSW(326427)] The C-terminal peptides blocked the 3889 Mapping of transcobalamin using antibodies S N Fedosov et al Table Specicity of monoclonal...

Ngày tải lên: 20/02/2014, 01:20

... developed Two antibodies showed clear recognition of plasma purified C4BP, MoAb 15 and 44 We therefore decided to proceed with more detailed analysis of these two antibodies All monoclonal antibodies ... measured and the concentration of antibody was calculated using an extinction coefficient (1%, cm) of 12.5 Antibodies were stored at °C after addition of 0.02% NaN3 Antibodies were tested using ... elucidate the influence of CCP2 on protein S binding, we made the effort to raise monoclonal antibodies against CCP1-2 with the aim to use them in structure–function analysis Two of 12 antibodies, MoAb...

Ngày tải lên: 17/03/2014, 09:20

... Enzymatic actions of P multocida toxin Results Analysis of enzymatic actions of PMT with Gaq Q209E-specific monoclonal antibodies According to a previous study [16], the deamidation of Ga by PMT results ... characteristics of PMT have not been analyzed as a result of the lack of an easily-administered assay to detect activity of the toxin In the present study, we developed rat monoclonal antibodies that ... Fig Isolation of Gaq Q209E-specific antibodies (A) Alignment of amino acid sequences of the switch region in the a subunits of mouse heterotrimeric GTPases by CLUSTALW Sequences of synthetic oligopeptides...

Ngày tải lên: 22/03/2014, 16:20

... Percentage of H-chain to sercin-1 0.0 4.4 ± 0.7 2.9 ± 0.1 a Copy numbers of mRNAs of L-chain and H-chains of the recombinant IgG, and sericin-1 per 10 ng of total RNA The indicated values are 10)5 of ... feasibility of using transgenic silkworms for the mass production of recombinant mAbs Silkworms have been used for the Production of mouse mAb by transgenic silkworms manufacture of silk in the ... proteins The absence of a-1,6-fucose comprises yet another reason supporting the use of this system in the production of mAbs The absence of a-1,6-fucose enhances the activity of antibody-dependent...

Ngày tải lên: 23/03/2014, 04:20

... RESULTS Monoclonal antibodies recognize epitopes in the N-terminal (ATPase) domain and in the C-terminal domain of MalK, respectively Monoclonal antibodies were prepared against the MalK subunit of ... differ from those of soluble MalK Fab fragments of 5B5 and 4H12 only slightly inhibit ATPase activity of MalK and of proteoliposomes Having established the binding properties of the mAbs, we analyzed ... ratios of 1.5 : and : 1, respectively (molecular mass of MalK, 40 kDa; molecular mass of complex, 171 kDa) In the case of proteoliposomes, this actually corresponds to a 12-fold molar excess of mAbs...

Ngày tải lên: 31/03/2014, 09:20

... lack of antibody binding [12] Here we evaluated binding of VN04-2 to a variety of H5 hemagglutinins (HA) independent of the HI assay, to determine the actual effects mutations in this region of ... receptor specificity of the hemagglutinin from an H5N1 influenza virus Science 2006, 312:404-410 Brown LE, Murray JM, White DO, Jackson DC: An analysis of the properties of monoclonal antibodies directed ... for expression of soluble HA trimers which could be purified by virtue of the carboxyl terminal hexa-histidine tag [14] Following introduction of the foldon sequence into the HA2 of A/Vietnam/1203/04...

Ngày tải lên: 20/06/2014, 01:20

... (shaking speed of 300 rpm in the presence of 12.5 g l-1 of YE and 15 g l-1 of AS) After a short Lag phase of about h, we witness an exponential phase of bacterial growth and maximum number of viable ... the production of the Bacillus sp MD2 phytase at 327 U ml-1 by fed-batch cultivation, the majority of earlier attempts with expression of Bacillus phytases in Escherichia coli have resulted in production ... equation obtained after the analysis of variance (ANOVA) provided the level of enzyme production as a function of the shaking speed (N) and the concentration (g l-1) of YE and AS (Table 4) The phytase...

Ngày tải lên: 20/06/2014, 20:20

... (shaking speed of 300 rpm in the presence of 12.5 g l-1 of YE and 15 g l-1 of AS) After a short Lag phase of about h, we witness an exponential phase of bacterial growth and maximum number of viable ... the production of the Bacillus sp MD2 phytase at 327 U ml-1 by fed-batch cultivation, the majority of earlier attempts with expression of Bacillus phytases in Escherichia coli have resulted in production ... equation obtained after the analysis of variance (ANOVA) provided the level of enzyme production as a function of the shaking speed (N) and the concentration (g l-1) of YE and AS (Table 4) The phytase...

Ngày tải lên: 21/06/2014, 19:20

... use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis) development and analysis of species specific and cross reactive monoclonal antibodies ... Naessens, J Analysis of monoclonal antibodies specific for the γδ TcR Vet Immunol Immunopathol 1996, 52, 275-283 Davis, W C., Davis, J E and Hamilton, M J 1995 Use of monoclonal antibodies and flow ... patterns of reactivity similar or identical to those in cattle The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis) Table Monoclonal...

Ngày tải lên: 07/08/2014, 15:20

... and functional mapping of epitopes on hog cholera virus with monoclonal Characterization of Antigenic Sites on the Rinderpest Virus N Protein Uusing Monoclonal Antibodies 65 antibodies J Gen Virol ... ELISA If it Characterization of Antigenic Sites on the Rinderpest Virus N Protein Uusing Monoclonal Antibodies 61 Table Mapping of antigenic sites in the N protein of RPV-LATC strain by competition ... R C., Mun, J B and Lee, N S Use of rabbit-passaged strains of the Nakamura LA Rinderpest virus for immunizing Korean cattle Bulletin of the Vet Res Lab Office of Rural Development 1962, 8(1),...

Ngày tải lên: 07/08/2014, 17:22

... level of expression of the LDM detected with MRB102A on lymphocytes was higher than Monoclonal antibodies specific for rabbit leukocyte differentiation molecules Table Reactivity of monoclonal antibodies ... AM, Naessens J Analysis of monoclonal antibodies specific for the γδ TcR Vet Immunol Immunopathol 1996, 52, 275-283 11 Davis WC, Davis JE, Hamilton MJ Use of monoclonal antibodies and flow cytometry ... population of lymphocytes in the spleen (Table 2, FC not shown) The level of expression of the LDM detected with RTH192A and BAQ44A differed on CD4 and CD8 T and B lymphocytes The level of expression of...

Ngày tải lên: 07/08/2014, 20:23

... localization of NPM1 may not be closely associated with NPM1 gene mutations probably because of NPM1 diffusion during the tissue fixation and the influence of fixatives [20] Thus, production of anti-NPM-mA ... detection of cytoplasmic dislocation of NPM1 by IHC has been performed using aspecific antibodies that bind both the NPM-wt and NPM-mA proteins In IHC assay labeling with this kind of mAbs, the ... prepare the anti-NPM-mA monoclonal antibodies (mAbs) as the primary antibody in IHC assay In 1999, Cordell et al prepared the first panel of mAbs associated with NPM1 protein, two of which recognized...

Ngày tải lên: 08/08/2014, 18:21