production of 7 amino cephalosporanic acid biochemie germany austria

... soybea1ns contain lower amounts of lysine, which is one of Microbial Production of Amino Acids in Japan 77 the essential amino acids for those livestocks The estimated amount of l-lysine produced in ... to belong to the genus Corynebacterium 73 Microbial Production of Amino Acids in Japan Table Amino acid production in Japan and the world in 1996 Amino acid Method Japan Glycine l-Alanine dl-Alanine ... putative component of a biotin-containing enzyme complex and has something to Microbial Production of Amino Acids in Japan 75 with fatty acid metabolism They reported that the disruption of this gene...

Ngày tải lên: 26/10/2013, 02:20

... Journal 276 (2009) 72 65 72 78 ª 2009 The Authors Journal compilation ª 2009 FEBS 72 71 Origin of rBAT and 4F2hc within the GH13 a-amylase family 72 72 ˇ ˇ ˇek M Gabrisko and S Janec FEBS Journal 276 (2009) ... 276 (2009) 72 65 72 78 ª 2009 The Authors Journal compilation ª 2009 FEBS ˇ ˇ ˇ M Gabrisko and S Janecek Origin of rBAT and 4F2hc within the GH13 a-amylase family FEBS Journal 276 (2009) 72 65 72 78 ... Journal 276 (2009) 72 65 72 78 ª 2009 The Authors Journal compilation ª 2009 FEBS 72 69 Origin of rBAT and 4F2hc within the GH13 a-amylase family ˇ ˇ ˇek M Gabrisko and S Janec Fig Evolutionary tree of...

Ngày tải lên: 18/02/2014, 13:20

... 3-hydroxyanthralinic acid differs from that in the pathway for 4 -amino3 -hydroxybenzoic acid The N-terminal amino acid sequence of the purified enzyme did not show significant levels of identity to sequences of 2-aminomuconate ... reaction mixture consisted of 2.9 mL of 100 mM sodium/potassium phosphate buffer (pH 7. 5), 0.1 mL of mM 4 -amino- 3-hydroxybenzoic acid, and 0.05 mL of the crude extract (35 75 % ammonia sulfate saturation) ... (B) The reaction mixture consisted of 2.9 mL of 100 mM sodium/potassium phosphate buffer (pH 7. 5), 0.1 mL of mM 4 -amino- 3-hydroxybenzoic acid, 0.1 mL of purified 4 -amino- 3-hydroxybenzoate 2,3-dioxygenase...

Ngày tải lên: 19/02/2014, 16:20

... 87. 1 ^ 22.2 (20) 114.9 ^ 21.5 (5) 71 .6 ^ 9.4 (8) 84.3 ^ 15.5 (4) 59.6 ^ 7. 8 (6) * 73 .1 ^ 10.6 (5) 120.0 ^ 1.6 (3) 58.1 ^ 7. 5 (6) * 62.8 ^ 4 .7 (4) 61.9 ^ 13.5 (6) 72 .5 ^ 24.0 (6) 75 .9 ^ 20.6 (7) ... alignments of 219 serpins showed that residue 53 is a Ser in 92% of the cases; residue 56 is a Ser in 74 % of the cases; residue 186 an Asn in 87% of the cases; and residue 334 a His in 80% of the ... ^ 4.4 (7) * 64 .7 ^ 14.2 (9) * 83.8 ^ 14.2 (6) 113 .7 ^ 28.0 (10) † 129.5 ^ 21.1 (3) 84.9 ^ 5.9 (3) 91.0 ^ 13.3 (3) 56.1 ^ 6.3 (3) * 1 27. 1 ^ 6.2 (3) † 129.6 ^ 2.5 (3) † 73 .1 ^ 5 .7 (3) 57. 8 ^ 5.3...

Ngày tải lên: 22/02/2014, 07:20

... Role of arginine 285 in the active site of Rhodotorula gracilis d -amino acid oxidase A site-directed mutagenesis study J Biol Chem 275 , 2 471 5–2 472 1 Massey V & Hemmerich P (1 978 ) Photoreduction of ... kinetic mechanism of pig kidney d -amino acid FEBS Journal 276 (2009) 4921–4932 ª 2009 The Authors Journal compilation ª 2009 FEBS 4931 Oxygen reactivity of D -amino acid oxidase 25 26 27 28 29 E Rosini ... 239, 39 27 3934 Porter DJ, Voet JG & Bright HJ (1 977 ) Mechanistic features of the d -amino acid oxidase reaction studied by double stopped flow spectrophotometry J Biol Chem 252, 4464–4 473 Pollegioni...

Ngày tải lên: 16/03/2014, 02:20

... 0.9490 P41212 82. 17 60 .73 90 120 1.9 35412 18054 97. 9 (86.1)a 15.0 (2.9)a 3.9 (32.4)a 72 .95 76 . 97 90 90 2.8 6 175 4 5424 92.3 (84.6)a 7. 8 (2.6)a 9.2 ( 37. 3)a 23.1 24.2 25.9 33.9 0.0 07 1. 27 0.009 1.56 ... mouse guanylate kinase J Biol Chem 277 , 3023630243 FEBS Journal 274 (20 07) 33633 373 ê 20 07 The Authors Journal compilation ê 20 07 FEBS 3 371 Substrate specicity of E coli CMP kinase A Oteru et al ... of the original molecule and part of its hydrogen- FEBS Journal 274 (20 07) 33633 373 ê 20 07 The Authors Journal compilation ê 20 07 FEBS 33 67 Substrate specicity of E coli CMP kinase A Oteru et al...

Ngày tải lên: 16/03/2014, 10:20

... case of r11c, however, seven out of 12 amino acids at the C-terminus of r11a are not conserved, including the amino acid to be modified (Phe in the case of r11a compared with Leu in the case of ... modification, three amino acids from the C-terminus However, the I2 superfamily may lack a recognition signal, and, consequently, the same pattern of conversion of l -amino acids into d -amino acids might ... confirmed to have a d -amino acid, and synthetic or expressed versions with both l -amino acids and d -amino acids at the correct locus in order to predict when and where d -amino acids may be found...

Ngày tải lên: 16/03/2014, 22:20

... Prod Rep 19, 76 1– 77 2 Pollegioni L, Piubelli L, Sacchi S, Pilone MS & Molla G (20 07) Physiological functions of d -amino acid oxidases: from yeast to humans Cell Mol Life Sci 64, 1 373 –1394 Umhau ... oxidase J Biol Chem 277 , 275 10– 275 16 Sacchi S, Rosini E, Molla G, Pilone MS, Ghisla S & Pollegioni L (2004) Modulating d -amino acid oxidase substrate specificity: production of an enzyme for analytical ... determination of all d -amino acids by directed evolution Protein Eng Des Sel 17, 5 17 525 Pollegioni L, Porrini D, Molla G & Pilone MS (2000) Redox potentials and their pH dependence of d -amino acid oxidase...

Ngày tải lên: 28/03/2014, 23:20

... total amount of lactic acid in the medium and the buffering capacity of the medium are known The amount of undissociated lactic acid is then further related to the total amount of lactic acid LaHtot ... Journal of Food Microbiology 73 (2002) 239–250 Table Parameter values for the model of Nicolaı et al (1993) for L lactis ¨ SL05 in BHIYEG medium at a temperature of 35 °C and an initial pH of 6 .7 Parameter ... Experimental validation of a 250 K.M Vereecken, J.F Van Impe / International Journal of Food Microbiology 73 (2002) 239–250 model for combined growth and metabolite production of lactic acid bacteria In:...

Ngày tải lên: 05/05/2014, 08:44

... :etontooF 253.0 ± 805.0 )lm/h · gµ( CUA 5.615 ± 5.152 )gk/h/l( F/totLC 1.064 ± 1.082 )gk/l( F/dV 133.0 ± 277 .0 )h( 2/1t 603.0 ± 3 57. 0 )h( a,2/1t 321.0 ± 291.0 )lm/gµ( xamC 5 47. 0 ± 286.2 )h( xamt 75 7.0 ... demrofrep LA1050-PV rof alumrof eninala dica onima eht yb ytilibaliavaoib fo tnemevorpmi ehT etadidnac gurdorp wen a rof yrotcafsitas erew amsalp ni noitartnecnoc fo ecnanetniam eht dna ,mrof ... :etontooF tot 2/1 d le 663.0 ± 446.0 )h( TRM 838.0 ± 518.1 )lm/ h · gµ( CMUA 855.0 ± 479 .2 )lm/h · gµ( CUA 361.1 ± 178 .6 )gk/h/l( LC 470 .3 ± 146.4 )gk/l( V 213.4 ± 860.6 )lm/gµ( C 452.0 ± 74 4.0...

Ngày tải lên: 07/08/2014, 23:22

... (IL) -7 induces rapid activation of Pyk2, which is bound to Janus kinase and IL-7Ralpha J Biol Chem 2000, 275 :70 60 -70 65 Loeser RF, Forsyth CB, Samarel AM, Im HJ: Fibronectin fragment activation of ... nitrate levels in media from explants treated with IL -7 (Figure 5b) Effects of age and OA on chondrocyte production of IL -7 Media was IL -7 collected 48 hours after changing to serum-free conditions ... together, the combination completely blocked IL -7 production (Figure 5a) This suggested that chondrocyte IL -7 production was a result of the combined effects of IL-1 and IL-6 To test this hypothesis,...

Ngày tải lên: 09/08/2014, 10:23

... J5/6; MIP-1α, K5/6; MIP-1γ, L5/6; RANTES, A7/8; SDF-1, B7/8; TCA-3, C7/8; TECK, D7/8; TIMP-1, E7/8; TIMP-2, F7/8; TNF-α, G7/8, sTNF R1, H7/8; sTNF R2, I7/8 Positive controls are located at positions ... ( 27% ) by TMP of the LPSinduced accumulation of MCP-1 in serum (Fig 8B) The effects of TMP on the growth of RAW 264 .7 cells Experiments summarizing the effects of TMP on the growth of RAW 264 .7 ... effect of TMP was not selective for production of PGE2 As shown in Figs 1C and 1D, 25 μM TMP inhibited the LPS-induced production of PGF2α and PGI2/prostacylin (as measured by production of the...

Ngày tải lên: 11/08/2014, 08:22

... identical contiguous amino acids For proteins from 39 to 79 amino acids in length, removal of each amino acid increased the number of pairs sharing only identical contiguous amino acids by less than ... one member of a protein pair was from 29 to 79 amino acids, the cumulative number of protein pairs meeting only the eight -amino- acid criterion, but not the >35% identity over 80 -amino- acid criterion, ... under 80 amino acids Effect of2 Effect of Sequence length on 8-mer-only hits for pairs with one protein under 80 amino acids Relationship between amino- acid length and the cumulative number of 8-mer-only...

Ngày tải lên: 13/08/2014, 13:22

... metabolism of amino acids This has been referred to as cross-pathway control since the induction of genes important for the biosynthesis of virtually all amino acids is independent of which amino acid ... synthesis of vitamins that are important cofactors for enzymes in pathways related to amino acids Expression of several genes encoding amino acid permeases are induced by Gcn4p 25 following amino acid ... target genes of Gcn4p focused on amino acid biosynthetic genes (76 ) These have been expanded by microarray studies in cells starved for branched-chain amino acids (78 ) or histidine (79 ) The latter...

Ngày tải lên: 24/08/2014, 12:49

... 4 .7 6.0 7. 7 9.5 430 76 5 8 67 1,3 17 1003 5.2 6.1 6.8 7. 2 338 668 963 1, 676 1004 5.5 6.6 8.0 10.2 316 542 71 3 75 5 1005 3.9 5.3 7. 1 9 .7 378 698 ,028 1,6 97 4.9 6.0 7. 7 9.4 373 6 67 939 ,393 Average ... mg/24 Df subjects Plasma Acid Number subjects hr Acid Uric of ml mg/lOO 14 354 ± 67 6.0 ± 22 3 37 ± 48 10 5.2 ± 28 352 ± 72 5.6 ± 37 331 ± 28 5.8 ± 75 20 392 ± 66 13 4 .7 ± 800 600 0.05) higher ... containing 0 -75 g of protein and, at the highest protein level, 0-8 g of added yeast ribonucleic acid in order to of urinary uric acid per milligram of purine added to the diet in the form of foods...

Ngày tải lên: 14/07/2015, 09:44

... Table 7. 1 Michael Addition of Oxindole 7- 1a to MVK 7- 2a Catalyzed by Amino Acid- derived Phosphines 311 Table 7. 2 Solvent Screening for the Michael Additions 312 Table 7. 3 Substrate Scope of the ... Oxindoles Catalyzed by Amino Acid- derived Bifunctional Phosphines 3 07 7.1 Introduction 308 7. 2 Results and Discussion 310 7. 2.1 Reaction Optimization 310 7. 2.2 Substrate Scope 313 7. 2.3 Mechanistic ... state model Scheme 5.1 173 [4+2] Annulations of isatin-derived alkene 5-7a catalyzed by mono amino acid- derived phosphines Scheme 5.2 172 202 The [4+2] annulation of 5-7a and 5-4a catalyzed by...

Ngày tải lên: 09/09/2015, 10:06

... lactic acid 1.3 Production of lactic acid 1.3.1 Chemical synthesis 1.3.2 Fermentation 1.3.3 Lactic acid microorganisms 1.3.3.1 Lactic acid bacteria 1.3.4 Metabolic pathways of lactic acid bacteria ... IV LIST OF TABLES Table Microorganisms used in the recent work of biotechnological production of lactic acid Table Recent investigation of xylose utilizing strains in the lactic acid production ... simplified flowchart of the potential products and technology 17 Fig 7A Time courses of lactic acid fermentation using glucose as the carbon source 23 Fig 7B Time courses of lactic acid fermentation...

Ngày tải lên: 16/10/2015, 11:57

... (mg/L) BOD (mg/L) CODCr (mg/L) pH 17 27 57 17 27 57 1020 960 880 800 580 1000 280 250 220 140 1020 960 870 79 0 580 1000 280 250 220 130 1360 1460 1280 860 390 370 350 210 2550 2500 2300 2100 1500 ... reaction at 250 C and MPa Journal of Water and Environment Technology, Vol.1, No.2, 2003 acid, % of glycolic acid and % of formic acid were detected in The amount of malic acid decreased, and was converted ... partial destruction of MCAA by hydrolysis, production of biodegradable glycolic acid and decomposition of glycolic acid to other organic acids were reported [11] Based on the results of ion chromatography,...

Ngày tải lên: 05/09/2013, 08:40

... order from Chemical Soft R&D Inc (Kyoto, Japan) 20 mg of each of the chemicals is dissolved into L of distilled water and mg-Cl/mg-C of sodium hypochlorite was added at pH 7. 0±0.2 After 24 hours, ... Research, 35 (7) , 16 27- 1634 Takanashi H., Kishida M., Hidaka Y., Kofune H., Ohyama K., Nakajima T and Ohki A (20 07) Mutagenicity of chlorinated agricultural chemicals, Proceeding of The 2nd IWA-ASPIRE ... observed in the mass spectrum, production of trichloro-3-methylphenol (TC3MP) in the sample of chlorinated fenitrothion was inferred To confirm the production of TC3MP, 3M4NP was chlorinated...

Ngày tải lên: 05/09/2013, 10:15

... Time of heatsetting[s] 15 30 45 60 90 120 240 PLA Warp[RT] % 58.05 53.44 56 .71 54 .73 55.41 51.83 56.89 PLA Weft[RT] % 44 .71 51.19 47. 22 48 .77 44. 57 45.90 50.22 PLA Mean 51.38 52.31 51. 97 51 .75 ... 2013, pp .79 7-806 Figure Effect of time of heatsetting on the tensile Extension EM [%] in Weft direction of knitted Ingeo™ Poly (lactic acid) [PLA] Figure Mean [Ā] of effect of time of heatsetting ... Table Linearity of load extension curve LT [-] Time of heatsetting [s] 15 30 45 60 90 120 240 LT Warp 0.963 0 .78 7 0.882 0.903 0 .78 7 0.820 0.915 LT Weft 0.915 0.935 0.865 0.828 0 .77 5 0 .78 3 0.925 LT...

Ngày tải lên: 05/09/2013, 16:10