production characterization and evaluation of bio nanoparticles

... effect of the hybrid gene transfer vector 33 Production, Characterization and Evaluation of Bio- nanoparticles CHAPTER PRODUCTION, CHARACTERIZATION, AND EVALUATION OF BIO- NANOPARTICLES 34 Production, ... Delivery Efficiency of Bio- nanoparticles 54 2.3.5 In Vitro Transduction Efficiency of Baculovirus-based Bionanoparticles 58 2.3.6 Zeta Potential of Baculovirus-based Bio- nanoparticles ... Formation and Characterization 88 3.3.2 Encapsulation of Baculovirus with Fiber 92 3.3.3 Transduction Ability of Bio- microfiber 95 3.3.4 Therapeutic Efficiency of Bio- microfiber...

Ngày tải lên: 12/09/2015, 09:10

... kind of Rice-fluid was proved to be devoid of bactericidal activity against strains of E coli, S aureus, S epidermidis, E faecalis and P aeruginosa, and strains of K pneumoniae and strains of K ... observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively (data ... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of the...

Ngày tải lên: 31/10/2012, 17:03

... between the 9th and 10th carbons of each 18-carbon chain Physicochemical characterization of the cationic lipids in lamellar assemblies with and without plasmid DNA and in media of various ionic ... laboratory and the structureactivity data collected for the various series contribute to the long-term goals of understanding the mechanism of lipofection, and the rational design of pharmaceutically ... amount of chloroform and loaded onto a silica gel column (26 ã 2.8 cm) The column was eluted sequentially with 100 mL chloroform, and 1, 3, 5, 7, 8, 9, 10, 12 (300 mL) and 15% methanol chloroform...

Ngày tải lên: 23/03/2014, 07:20

... only three of the nine HIV-1 genes and relies on four separate transcriptional units for the production of transducing particles This system offers significant advantages for its biosafety and allows ... NP-40, and lysed by three cycles of freezing and Ó FEBS 2002 2766 P Di Natale et al (Eur J Biochem 269) thawing Lysates were cleared by centrifugation at 6200 g for in an Eppendorf microfuge and ... increased enzymatic activity, both in the absence of polybrene (means of 6, 22 and 117 nmolÆh)1Æmg)1) or in the presence of polybrene (means of 20, 32, and 155 nmolÆh)1Æmg)1) The IDUA activity obtained...

Ngày tải lên: 24/03/2014, 04:21

... and Spm, and the highest percentages of Put were found in s-ivNAP Recovery of Spm, the major constituent of l-ivNAP, progressively increased with the ivNAP size In contrast, recovery of Put and ... et al ivNAPs of the molecular masses of several coeluting compounds On the other hand, attempts to confirm the proposed molecular formulas by means of ‘soft’ MS techniques (MALDI-TOF and ESI-MS, ... derivatizing the single standard polyamines (aliquots ranging between 0.125 and 0.5 lg for Put and Spd, and between 0.5 and lg for Spm) Each standard sample was run in triplicate, and the mean value...

Ngày tải lên: 29/03/2014, 23:20

... prediction in locomotion Journal of Biomechanics 1981, 14(11):793-801 Crowninshield RD, Johnston RC, Andrews JG, Brand RA: A biomechanical investigation of the human hip J Biomech 1978, 11(1-2):75-85 ... group doff was 32 mm, whereas in group it was set at 0.83*dm, (and since dm ranged between 46 and 49 mm, doff therefore ranged between 38 and 41 mm) The Fap was applied in three phases of 1000 ... University of British Columbia, Vancouver, Canada, 4Department of Mechanical and Aerospace Engineering, Carleton University, Ottawa, Canada and 5Department of Mechanical Engineering, University of British...

Ngày tải lên: 20/06/2014, 04:20

... mass spectroscopy and elemental analysis The IR spectra of compounds (e) and (f) showed the absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, ... Compounds (a) and (d) showed 85.44% and 92.13% protection at µM level, 92.94% and 94.76% protection at 10 µM level, and 94.69% and 96.39% protection at 15 µM respectively, hr after the induction of oxidation ... AA was also determined and compared with those of the synthesised compounds (Fig 3) The antioxidant activity of BHA and AA was still lower than that of the compounds (a) and (d) In general, the...

Ngày tải lên: 07/08/2014, 14:20

... development Biol Reprod 1994, 50,390-400 Gordon, I and Lu, K H Production of embryos in vitro and its impact on livestock production Theriogenology 1990, 33, 77-87 10 Gordon , I Laboratory production of ... J and Lu, K H Effects of different oestrus stages of ovaries and sizes of follicles on generation of bovine embryos in vitro Theriogenology 1990, 33,335 Taylor, C and Rajamahendran, R Effect of ... C and Suzuki, T Effect of presence of CL on ovary on the oocyte number, cleavage rate and blastocyst production in vitro in cattle Theriogenology 1995, 43, 169 Eyestone, W H and First, N L Characterization...

Ngày tải lên: 07/08/2014, 17:22

... test at the level of 5% + Callusing at the basal end Table III Analysis of variance for the effects of BAP, Ads, NAA and IAA on production of multiple shoots (A) and average number of shoots per ... 1b) Analysis of variance for the effects of cytokinins and auxins on production of multiple shoots and average number of shoots/explant was presented in Table III The interaction of two cytokinins ... were excised and cultured on half-strength MS basal salts supplemented with 0, 0.25, 0.5, 1.0 and 1.5 mg/L of IAA, IBA and NAA alone or in combination for induction of rooting In case of rooting...

Ngày tải lên: 08/08/2014, 01:21

... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant ... proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by...

Ngày tải lên: 09/08/2014, 14:22

... http://www.virologyj.com/content/8/1/26 Page of 120 100 80 60 40 20 Cont SNC SNH Concentration (ug) Figure Anti-HCV activity of chloroform and n-hexane fraction of Solanum nigrum Huh-7 cells were incubated with HCV serum and 100 ... for characterization of antiviral compounds Extracts were weighed and their %age yield was calculated Stock solution preparation 50 mg of each dried plant extract was suspended in one ml of Dimethyl ... plate was sealed and kept at 37°C in an atmosphere of 5% CO2 for 24 h After the herbal extracts treatment was over, removed the media and test compounds 100 μl fresh media and 20 μl of MTT solution...

Ngày tải lên: 11/08/2014, 21:21

... indicated by the high peak of synergy above the flat plane of additivity (Fig 2A and 2B) and overall synergy volumes of 181 μM2% and 267 μM2%, respectively (Table 1) The combination of FTC+EFV was moderately ... and FTC-TP [30]; and (2) DEC or DEC-like complex formation by TFV-terminated DNA and HIV-1 RT in the presence of the FTC-TP or EFV, and by FTC-MP-terminated DNA and HIV-1 RT in the presence of ... employees of Gilead Sciences, Inc and shareholders of the company 11 Authors' contributions JYF designed and conducted the biochemical assays and drafted the manuscript JKL carried out a portion of...

Ngày tải lên: 12/08/2014, 23:21

... confirmed by WB (Figure 3) Repressors of transcription SIN3/HDAC SIN3/HDAC is composed of SIN3A, SAP30, SAP18, HDAC-1 AND -2 and RbAp46/48 and remarkably, all of Page of 18 (page number not for citation ... (KPNA2, KPNB1, XPO1, RANGAP1 and RAN) and microfilaments/tubule (TUBB3, TUBBA2, NUMA1 and DNCL1) LAMINA The nuclear lamina lines the INM and is composed of lamins and NE lamin binding proteins ... digestion Briefly gel bands were washed with sequential additions of ammonium biocarbonate and acetonitrile (ACN) buffers and cysteine residues were reduced and alkylated using DTT and IAA Samples were...

Ngày tải lên: 12/08/2014, 23:21

... 1988;Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion of 3T3-L1 cells and production of unorthodox laminin complex J Biol Chem (31):16163-16169 116 Mitsi ... of the intracellular environment Curr Opin Struct Biol (1):114-119 28 Zimmerman SB, Minton AP 1993;Macromolecular crowding: biochemical, biophysical, and physiological consequences Annu Rev Biophys ... 2008;Expression and hydroxylamine cleavage of thymosin alpha concatemer Journal of biomedicine & biotechnology :736060 63 Hanada K, Dennis JE, Caplan AI 1997;Stimulatory effects of basic fibroblast...

Ngày tải lên: 11/09/2015, 09:16

... cancer cells Page of 10 Results Synthesis and characterization of Cu-loaded polymeric nanoparticles Carboxylate-functionalized, acrylate-based nanoparticles were synthesized via standard microwave-assisted, ... through the slow leaching of metal ions from the particle core [19-21,25] Increased ROS production is capable of inducing biological damage and has been linked to a variety of disease states including ... Figure Characterization of CuCNPs (A) SEM image of CuCNPs, (B) EDX spectra of CNPs before (left) and after (right) addition of Cu, (C) XPS spectra for CuCNPs (right) and control particles containing...

Ngày tải lên: 21/06/2014, 02:20

... absence of UV irradiation: a ZnO nanoparticlefree (control), b 2.5 lg/mL of ZnO nanoparticles, c 5.0 lg/mL of ZnO nanoparticles and d 10.0 lg/mL of ZnO nanoparticles; b The 2.5 10 Concentration of ... c ZP5 ZnO nanoparticles; d ZP5 ZnO nanoparticles and daunorubicin; e ZP6 ZnO nanoparticles; f ZP6 ZnO nanoparticles and daunorubicin; g ZP7 ZnO nanoparticles; h ZP7 ZnO nanoparticles and daunorubicin ... of SMMC-7721 cells exposure to ZP5 ZnO nanoparticles a in the absence of UV irradiation: a ZnO nanoparticlefree (control), b 2.5 lg/mL of ZnO nanoparticles, c 5.0 lg/mL of ZnO nanoparticles and...

Ngày tải lên: 21/06/2014, 18:20

... distribution of the hydrodynamic diameter of bare Gnps and Gnps conjugated antibiotics (b) represents the intensity distribution of the hydrodynamic diameter of the free Gnps and the antibiotics conjugated ... bactericidal activity of the antibiotics Using the combined reducing property of antibiotics and borohydride, antibiotics were conjugated with Gnps The interaction between antibiotics and Gnps is likely ... Preparation of Conjugated Gold Nanoparticles using Antibiotics as Template The combined reducing property of sodium borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was...

Ngày tải lên: 22/06/2014, 06:20

... distribution of the hydrodynamic diameter of bare Gnps and Gnps conjugated antibiotics (b) represents the intensity distribution of the hydrodynamic diameter of the free Gnps and the antibiotics conjugated ... bactericidal activity of the antibiotics Using the combined reducing property of antibiotics and borohydride, antibiotics were conjugated with Gnps The interaction between antibiotics and Gnps is likely ... Preparation of Conjugated Gold Nanoparticles using Antibiotics as Template The combined reducing property of sodium borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was...

Ngày tải lên: 22/06/2014, 19:20

... effect of [PDDA] on sizes and zeta-potentials of DODAB BF/AmB/CMC assemblies at mM DODAB, 0.005 mM AmB and mg/mL CMC is on Figure The region of PDDA concentrations for size minimization and high ... in zeta-potential and a decrease of % of cell viability (Table 2) Table summarized the different properties of assemblies at low and high P One should notice that coverage of a drug particle ... purposes) Journal of Nanobiotechnology 2008, 6:6 http://www.jnanobiotechnology.com/content/6/1/6 Table 2: Sizing, zeta-potential and antifungal activity of drug, cationic lipid, and polyelectrolyte(s)...

Ngày tải lên: 11/08/2014, 00:22

... synthesis and characterization of lipid shell and polymer core nanoparticles (LPNPs) are discussed in Chapter while the synthesis and characterization of transferrin conjugated lipid shell and polymer ... studied Nanoparticles of PLGA as the core and various lipids as the shell were produced by nanoprecipitation method Transferrin was used as the targeting ligand Series of characterization of the nanoparticles ... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude...

Ngày tải lên: 09/10/2015, 11:18