production and purification from plants fig 1a

... translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was performed from cell lysates ... antibodies and the 57 kDa band was again detected (Figure 5, lanes to 5) The anti-ricin antibodies also detected other minor bands visible in the non-retained and wash fractions (Figure 5, lanes and ... p24 6xHIS RTB p24 Figure of recombinant proteins expressed in E coli and immunodetection Purification Purification of recombinant proteins expressed in E coli and immunodetection Purification was...

Ngày tải lên: 12/08/2014, 04:21

... offering a cleaner environment 1.2 H2 production and purification needs In thermochemical processes for hydrogen production from fossil fuels, separation and purification is a critical technology ... methanol production (8%) and in the chemical and metallurgical industries (4%) With greater emphasis placed on environmental sustainability, energy cost and security (both for stationary and transport ... of Colloid and Interface Science 314 (2007) 589–603 Fig Hydrogen and helium permeance (with the feed of 1:1 hydrogen and helium mixture) through a 200 nm thick Pd/Ag membrane before and after...

Ngày tải lên: 13/05/2014, 01:01

... expressing PPV VP2 in vitro and to purify the fusion protein Materials and methods 2.1 Cells, virus, and reagents Strain 20-06 of PPV was isolated from a dead fetus delivered from a sow diagnosed with ... contributions GY and HZ carried out the molecular studies, and drafted the manuscript SC participated in the design of the study and conceived of the study, and participated in its design and coordination ... plasmids pFastpVP2 and recombinant bacmid The recombinant plasmids pFastpVP2 and B-pFastpVP2 were identified by BamHI and SalI enzyme digestion The fragments were about 1800 bp and 4775 bp, respectively,...

Ngày tải lên: 11/08/2014, 21:21

... Tyrosinase enzymes and their genes have previously been characterized from bacteria, fungi, plants and mammals The most extensively investigated fungal tyrosinases, from both a structural and a functional ... Secreted tyrosinase from Trichoderma reesei by relatively low production yields of the enzymes In this article, the cloning, production and characterization of a novel tyrosinase from the filamentous ... and use in applications We have reported here for the first time the production, purification and characterization of a novel tyrosinase from the well-known protein producer T reesei The high production...

Ngày tải lên: 19/02/2014, 06:20

... separated from each other by CCC Since K2 and K4 were close to K3 and K5, respectively, curcumol (2) and germacrone (3), and curzerene (4) and 1,8-cineole (5) were hardly separated from each ... or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents systems, the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ... H3-7), 1.21 (6H, s, H3-9 and H3-10 ), 1.36 (1H, m, H-4), 1.45 (2H, m, Ha-3 and Ha-5), 1.47 (2H, m, Ha-2 and Ha-6 ), 1.61 (2H, m, Hb-2 and Hb-6 ) and 2.00 (2H, m, Hb-3 and Hb-5 ) The 13C NMR spectrum...

Ngày tải lên: 07/03/2014, 21:20

... Invitrogen (Paisley, UK) and New England Biolabs (Ipswich, MA, USA) Pfu Turbo and T4 DNA ligase were purchased from Invitrogen and Stratagene (Amsterdam, the Netherlands), respectively For heterologous ... dehydrogenase from Pyrococcus furiosus R Machielsen and J van der Oost TTE2405) and a TDH from E coli (44% identity, tdh) [15,16] These sequences were used to make an alignment (Fig 1) Highly ... strands Production and purification of ADH E coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a single colony was used to inoculate mL Luria–Bertani medium with kanamycin and...

Ngày tải lên: 16/03/2014, 14:20

... et al Nitrile-forming proteins from plants and insects Table Nitrile and epithionitrile formation from different glucosinolates in the presence of myrosinase, ESP and NSP in vitro Assays were performed ... Nitrile-forming proteins from plants and insects M Burow et al Fig Predicted protein structure of A thaliana Ler ESP Amino acid sequence of A thaliana ESP (GenBank accession number AAL14623) and Kelch motif ... Nitrile-forming proteins from plants and insects 10 11 12 13 14 We thank Andrea Bergner for technical assistance, Michael Reichelt for providing intact glucosinolates, and the Max Planck Society...

Ngày tải lên: 23/03/2014, 11:20

... OCR process and that are ready for spellchecking and layout Final Final Documents that are in final format and can be returned Production and management of electronic documents - From hardcopy ... the documents, and producing the final version Production and management of electronic documents - From hardcopy to electronic document - page The process It is possible to scan and OCR in a single ... them and make notes by hand, or you can send the file to your colleagues, so that they can update and resend it to you Anyway, it’s important to update the spreadsheet regularly Production and...

Ngày tải lên: 24/03/2014, 03:20

... [D-erythro-6-(1’,2’,3’-trihydroxypropyl)-pterin] production [6] and trp degradation [7,8] In vitro evidence demonstrated that both IFNb and IFNg induce neopterin production [9] and activate the enzyme indoleamine ... IFNb -1a at a dose of 44 or 22 mcg, sc three times weekly for years To minimize adverse effects, IFNb -1a was titrated as follows: 8.8 mcg at weeks and of therapy, 22 mcg at weeks and 4, and, for ... as an internal standard The coefficient of variation of intra- and interassay determinations for trp and kyn was below 5% Recovery of trp and kyn was determined by measuring trp and kyn in 20 μl...

Ngày tải lên: 18/06/2014, 19:20

... [D-erythro-6-(1’,2’,3’-trihydroxypropyl)-pterin] production [6] and trp degradation [7,8] In vitro evidence demonstrated that both IFNb and IFNg induce neopterin production [9] and activate the enzyme indoleamine ... IFNb -1a at a dose of 44 or 22 mcg, sc three times weekly for years To minimize adverse effects, IFNb -1a was titrated as follows: 8.8 mcg at weeks and of therapy, 22 mcg at weeks and 4, and, for ... as an internal standard The coefficient of variation of intra- and interassay determinations for trp and kyn was below 5% Recovery of trp and kyn was determined by measuring trp and kyn in 20 μl...

Ngày tải lên: 20/06/2014, 03:20

... 1,2-monooxygenase from P putida NCIMB 10007 in Escherichia coli followed by simplified purification via affinity chromatography and characterization of the enzyme Material and methods Enzymes, chemicals and ... media Pfu+-polymerase was obtained from Roboclon (Berlin, Germany) and dNTPs from Roth (Karlsruhe, Germany) Restriction enzymes were obtained from New England Biolabs (Beverly, MA, USA) For SDS-PAGE ... DNA -purification from PCR, the MinElute PCR -purification Kit by Qiagen (Hilden, Germany) was used Furthermore the Miniprep Kit from Qiagen was used for plasmid purification HisTrap mL FF columns and Sephadex...

Ngày tải lên: 21/06/2014, 02:20

... of maize cobs and groundnut shells in the ratio of 1:1, 1:2, 2:1, 1:3 and 3:1 were leached and the hydrolyzed samples were filtered The resulting filtrate was analyzed for glucose and the best ... Collection and Processing of Substrate Used Maize cobs and groundnut shells were collected in polythene bags from the farm of Government Technical School, Kontagora, Niger State, Nigeria and transported ... glucose yield with time as shown in Tables and Results and Discussion Acid hydrolysis of maize cobs and groundnut shells at different acid concentrations and at ambient temperatures showed an increase...

Ngày tải lên: 06/07/2014, 18:20

... antigens HLA-DRB1*0404 and HLA-DRB1*0401 with disease susceptibility and severity [16,17], and by the high-level expression of MHC class II molecules and both CD28 ligands, CD80 and CD86, in the inflamed ... controls expression of type interleukin-10 receptor and from on Cell surface from on CD4+ T cells from patients with rheumatoid arthritis (RA) and from healthy controls (HC) Peripheral blood mononuclear ... (PB) CD4+ T cells from RA patients Materials and methods Patients and samples The total patient population consisted of 32 patients with RA (25 women and seven men; mean ± standard deviation age,...

Ngày tải lên: 09/08/2014, 01:24

... Alternative Energy from Palm Oil and Oil Crops, Faculty of Engineering, Prince of Songkla University, Hat Yai, Thailand from production of biodiesel by transesterification of waste cooking oils and palm ... addition, 40:1 COD:TKN ratio was also investigated Figure Biogas production rate from anaerobic batch digestion Figure Cumulative biogas production from anaerobic batch digestion 3.3 The optimum ... composition is 72% for glycerol substrate [13] Figure Biogas production from anaerobic semi-continuous digestion with COD:TKN ratio of 40:1 Figure Biogas production from anaerobic semi-continuous digestion...

Ngày tải lên: 09/09/2015, 10:17

... post-induction at different time (0, 1, 2, 3, 4, and 24 h) intervals Equal volume from each samples (20 μl) was analyzed on 12% SDS–PAGE 1.3 Biomass preparation and extraction of soluble cHSPA6 The biomass ... reached basal level and the wash was collected To elute bound protein, gradient was set to 50%B (50 mM Tris, 500 mM imidazole and 500 mM sodium chloride, pH 7.5) at 0.5 ml/min and the protein was ... HSPA6 is well folded, soluble and resistant to E coli cytosolic proteases Figure Post-induction incubation vs growth in the shake flask culture 2.3 Biomass preparation and extraction of soluble cHSPA6...

Ngày tải lên: 24/10/2016, 14:24

... Ophthalmology: Delair E, Monnet D, Grabar S, Dupouy-Camet J, Yera H, Brézin AP Respective roles of acquired and congenital infections in presumed ocular toxoplasmosis Am J Ophthalmol 2008;146:851-5) • Optical ... treatment of ocular toxoplasmosis We hope that this special issue will be interesting to readers and provides researchers with timely update on various topics in this important field Conflict of...

Ngày tải lên: 03/11/2012, 11:11

... at m/z 483 [M+H]+ in the positive and an ion peak at m/z 481 [M-H]+ in the negative spectra All NMR assignments of were made carefully from HSQC and HMBC and from the comparison with those of ... horseradish peroxidases and lipoxygenase and antihepatotoxic agent [5] Compound was obtained as yellow crystals from methanolic extract of the fruits of S marianum (L.), mp 155 - 157oC and had similar ... the methylene bearing oxygen atom was at 62.94 and methoxyl group was at 55.64 All NMR assignments of were made from analysis of HSQC, HMBC spectra and by comparison with those of isolated compound...

Ngày tải lên: 07/11/2012, 15:53

... livestock production 1980-2007 (FAO, 2009) 32 Livestock in ASIAN region, a casestudy • Cambodia • Lao PDR • Thailand • Vietnam 33 Land and population Population Density Population Land area Cambodia ... its human population with out destroying the enviroment and depleting natural resources???” Land used by region and country group, 1961,1991 and 2007, (FAO, 2009) Food security The World Food Summit ... deriver from livestock products per capital 1961-2005 FAO, 2009 14 Per capita consumption of livestock products by region, country group and country 1980 and 2005 FAO (2009) 15 Meat consumption and...

Ngày tải lên: 08/04/2013, 10:35

... to its customers than other PC makers and can quickly identify, and adjust to, changing customer demand It is better able to forecast future demand and plan production schedules accordingly Once ... Teamwork and participation Supplier B Quick setups Lean Production Quality at the and orders source (jidoka) Continuous improvement (kaisen) Pull approach and kanban production control Demand forecast ... be very fast and responsive This requires quick setups for production and rapid turnaround on orders from suppliers The Internet will facilitate this aspect of JIT as well As lot and order sizes...

Ngày tải lên: 12/08/2013, 07:56

... mutagenic CBP(s) from the chlorinated fenitrothion sample and some related samples by using GC/MS in the present study MATERIALS AND METHODS Chemicals and Sample Preparation Fenitrothion and fenitrooxon ... calculated from the slope of dose-response lines (Takanashi and Urano, 1998; Kishida et al., 2010) RESULTS AND DISCUSSION Qualitative and Quantitative Information Using the quadrupole GC-MS, productions ... was scraped from the plate and the substance was eluted from the silica gel by suspending the gel three times in methanol The eluates were evaporated to dryness under a nitrogen stream and the solvent...

Ngày tải lên: 05/09/2013, 10:15