... washings (30 seach) in ddw; one washing (30 min) in 12 mm AgNO3underlamp illumination; washing in ddw; very fast washing in 0.28 mm Na2CO3 and 1% formaldehyde; and washing in ddw and store-developed ... fol-lowing washings were done: one washing (30 min) in 50%methanol and 12% acetic acid; two washings (10 min each) in 10% ethanol and 5% acetic acid; one washing (10 min) in 3.4 mm K2Cr2O7 and 3.2 ... d,l-homocysteine(200 mgặkg)1ặday)1) in the drinking water with NaCl Piinjections (n ẳ 8); and (d) d,l-homocysteine (200mgặkg)1ặday)1) in the drinking water with AS101(1.5 lgặg)1) injections...
... Thesefindings indicate that elevated collagen type II, collagen type I and YKL40 mRNA expression levels are early and sensitivemeasures of ACLT-induced joint instability independent of acertain ... manyparts of the joint and, in keeping with this, col II and YKL40expression rose in all locations after ACLT in our study. Sur-prisingly, we detected significant differences in col II and col I expression ... sensi-tive and quantitative molecular analysis of the response ofarticular cartilage to increased joint instability and alteredmechanical loading, and thus to gain more insight into thevery...
... play a role in RA, includingextracellular signal (mitogenic)-regulated protein kinase(ERK)1/2; the stress-activated protein kinases, also called c-Jun NH2-terminal kinases (JNKs); and the p38 ... path-way is involved in the TNF-α-mediated increase in Cyp7bactivity in human FLS. Signaling pathways that mediate theeffects of TNF-α include mitogen-activated protein kinases(MAPKs) and nuclear ... mitogen-activated protein kinase inhibitorsPD98059 and SB203580 had no effect. In addition, inhibitionof Cyp7b mRNA expression and activity was observed withSN50, PSI and SP600125, suggesting that NF-κB and...
... protein involved in mRNA splicing andprotein synthesis*YDR194c MSS116 Mitochondrial RNA helicase, required for splicing of group II introns*YDR296w MHR1 Involved in repair, recombination and ... GFP-tagged proteinin mitochondriaThe list indicates systematic and standard names of genes required for protein translation activity in class I and III pet mutants. The cellular roles of the proteins ... according to data from the Saccharomyces Genome Database [19] and manual annotation. Red indicates mitochondrial proteins, green known extra-mitochondrial proteins, grey unknown proteins, and...
... the24-h changes in plasma prolactin levelsin female lactating rabbitsFigure 124-h changes in plasma prolactin levelsin female lac-tating rabbits. Groups of 6–7 animals maintained with their ... rhythms in plasma prolactin, growth hormone, thyro-tropin, insulin, follicle-stimulating hormone, luteinizing hor-mone and testosterone in rats. Life Sci 2000, 66:1969-1977.11. Esquifino AI, ... neu-roendocrine and circadian stimulus like the stimulationof nipples by the lactating pup affect the 24-h changes in gonadotropin and prolactin release?; (ii) could thechanges in circulating hormone levels...
... containing serine/threonine phosphatase inhibitors and one containing tyrosine phosphatase inhibitors) wasalso used. Protein ki nases were digested wi th lysyl endo-peptidase and trypsin and ... Purifi-cation and fusion proteins were expressed in Escherichiacoli. The kinase assay was carried out incubating with dif-ferent types of protein phosphat ase inhibitors in order toincrease the levels ... The mass and lists of fragment masses for each peptide are scanned against protein sequencedatabases, resulting in a list of identified peptides and proteins. The lists of proteins and their...
... Abnormalities in the dopamine system in schizophrenia may lie in altered levels of dopamine receptor-interacting proteins. Biol Psychiatry 2004, 56:427-40.14. Stoof JC, Kebabian JW: Opposing roles ... type. Since PC12 cells arecommonly used as a dopaminergic model, in order toinvestigate involvement of NCS-1 in dopaminergic intra-cellular signaling pathways, we established PC12 cell lineoverexpressing ... do not change levels of both proteins in rat's brain. However, since NCS-1 and DARPP-32 levels are not altered in wild type rats, we treated wild type PC12 cells (PC12 WT) and PC12 cells...
... protein intake. Actual protein intake was com-parab le to perceived protein needs (p = 0.16) and to theFigure 2 Actual Protein Intake. The RDI, maximum beneficial levelof protein intake, and ... Protein metabolism during intensive physical training in the young adult. Am JClin Nutr 1975, 28:29-35.12. Tarnopolsky MA, MacDougall JD, Atkinson SA: Influence of protein intake and training status on ... differed in terms of protein content. Actual protein intake was quantified using 3-day food records and nutrient analysis. Single samplet-tests were used to compare protein intake and perceived protein...
... proliferation, invasion into living cells and starting apoptosis, a defect in the synthesis of apolipoprotein B resulting in accumulation of lipids inside the cell, and downregulation of the total protein ... tried to find a possible link between the elevated serum levels of neurokinin A and anti-ribosomal P protein antibodies in autism. In this work, patients with elevated serum neurokinin A levels ... levels of neurokinin A and anti-ribosomal P protein antibodies was also studied. Methods: Serum neurokinin A and anti-ribosomal P protein antibodies were measured in 70 autistic children in...
... 1Decreased levels of soluble amyloid β -protein precursor and β-amyloid protein but increases of intrathecal axonal degradation products and TGF-β in patients with cerebral lupus. (a) Cerebrospinal ... SLE and age-matchedcontrols were evaluated clinically, with magnetic resonanceimaging of the brain and CSF analyses. Levels of tau, amyloidprecursor protein (APP), β-amyloid protein (Aβ42), and transforming ... without MRI-verified brain pathology. Thisfinding, together with increased intrathecal levels ofTGF-β, indicates an ongoing destructive parenchymatousprocess in the SLE brain. In addition, the present...
... the protein bands of interest. The same amount of protein was loaded onto each lane.Figure 3Content of osteogenic protein 1 proteinin synovial fluid samplesContent of osteogenic protein 1 protein ... The inclusion ofother catabolic agents, such as proinflammatory cytokines and proteinases, in such analyses may provide a better under-standing of the processes occurring in the articular joint.ConclusionTaken ... theacquisition, analysis and interpretation of the data andin draft-ing the manuscript. MM was involved in sample acquisition and analysis of the data. BK was involved in the development and adaptation...
... steady-state levels were increased in gun2 (allele of hy1 - required for phytochromobilinsynthesis from heme) [35], gun4 (mutant in the Proto-porphyrin I X- and Mg-Protoporphyrin IX-binding pro-tein) ... precursorproteins. Plant J 2001, 27:59-65.66. Bradford MM: A rapid and sensitive method for the quantitation ofmicrogram quantities of protein utilizing the principle of protein- dyebinding. Analyt ... targeting det erminants for chloroplasts and mito-chondria are usually located at the N-terminus of the protein; therefore, a fusion protein with GFP fused tothe C-terminus of TSPO was made. Using...