... washings (30 s each) in ddw; one washing (30 min) in 12 mm AgNO 3 under lamp illumination; washing in ddw; very fast washing in 0.28 mm Na 2 CO 3 and 1% formaldehyde; and washing in ddw and store-developed ... fol- lowing washings were done: one washing (30 min) in 50% methanol and 12% acetic acid; two washings (10 min each) in 10% ethanol and 5% acetic acid; one washing (10 min) in 3.4 mm K 2 Cr 2 O 7 and 3.2 ... d,l-homocysteine (200 mgặkg )1 ặday )1 ) in the drinking water with NaCl P i injections (n ẳ 8); and (d) d,l-homocysteine (200 mgặkg )1 ặday )1 ) in the drinking water with AS101 (1.5 lgặg )1 ) injections...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo y học: "Early and stable upregulation of collagen type II, collagen type I and YKL40 expression levels in cartilage during early experimental osteoarthritis occurs independent of joint location and histological grading" ppt
... These findings indicate that elevated collagen type II, collagen type I and YKL40 mRNA expression levels are early and sensitive measures of ACLT-induced joint instability independent of a certain ... many parts of the joint and, in keeping with this, col II and YKL40 expression rose in all locations after ACLT in our study. Sur- prisingly, we detected significant differences in col II and col I expression ... sensi- tive and quantitative molecular analysis of the response of articular cartilage to increased joint instability and altered mechanical loading, and thus to gain more insight into the very...
Ngày tải lên: 09/08/2014, 06:22
Báo cáo y học: " Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b" doc
... play a role in RA, including extracellular signal (mitogenic)-regulated protein kinase (ERK)1/2; the stress-activated protein kinases, also called c- Jun NH 2 -terminal kinases (JNKs); and the p38 ... path- way is involved in the TNF-α-mediated increase in Cyp7b activity in human FLS. Signaling pathways that mediate the effects of TNF-α include mitogen-activated protein kinases (MAPKs) and nuclear ... mitogen-activated protein kinase inhibitors PD98059 and SB203580 had no effect. In addition, inhibition of Cyp7b mRNA expression and activity was observed with SN50, PSI and SP600125, suggesting that NF-κB and...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo y học: "Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisia" pot
... protein involved in mRNA splicing and protein synthesis *YDR194c MSS116 Mitochondrial RNA helicase, required for splicing of group II introns *YDR296w MHR1 Involved in repair, recombination and ... GFP-tagged protein in mitochondria The list indicates systematic and standard names of genes required for protein translation activity in class I and III pet mutants. The cellular roles of the proteins ... according to data from the Saccharomyces Genome Database [19] and manual annotation. Red indicates mitochondrial proteins, green known extra-mitochondrial proteins, grey unknown proteins, and...
Ngày tải lên: 09/08/2014, 20:20
Báo cáo y học: "Effect of litter separation on 24-hour rhythmicity of plasma prolactin, follicle-stimulating hormone and luteinizing hormone levels in lactating rabbit does" pdf
... the 24-h changes in plasma prolactin levels in female lactating rabbitsFigure 1 24-h changes in plasma prolactin levels in female lac- tating rabbits. Groups of 6–7 animals maintained with their ... rhythms in plasma prolactin, growth hormone, thyro- tropin, insulin, follicle-stimulating hormone, luteinizing hor- mone and testosterone in rats. Life Sci 2000, 66:1969-1977. 11. Esquifino AI, ... neu- roendocrine and circadian stimulus like the stimulation of nipples by the lactating pup affect the 24-h changes in gonadotropin and prolactin release?; (ii) could the changes in circulating hormone levels...
Ngày tải lên: 10/08/2014, 09:20
Báo cáo y học: "Discovering and validating unknown phosphosites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools" doc
... containing serine/threonine phosphatase inhibitors and one containing tyrosine phosphatase inhibitors) was also used. Protein ki nases were digested wi th lysyl endo- peptidase and trypsin and ... Purifi- cation and fusion proteins were expressed in Escherichia coli. The kinase assay was carried out incubating with dif- ferent types of protein phosphat ase inhibitors in order to increase the levels ... The mass and lists of fragment masses for each peptide are scanned against protein sequence databases, resulting in a list of identified peptides and proteins. The lists of proteins and their...
Ngày tải lên: 10/08/2014, 09:22
Báo cáo khoa hoc:" Lack of effects of typical and atypical antipsychotics in DARPP-32 and NCS-1 levels in PC12 cells overexpressing NCS-1" doc
... Abnormalities in the dopamine system in schizophrenia may lie in altered levels of dopamine receptor-interacting proteins. Biol Psychiatry 2004, 56:427-40. 14. Stoof JC, Kebabian JW: Opposing roles ... type. Since PC12 cells are commonly used as a dopaminergic model, in order to investigate involvement of NCS-1 in dopaminergic intra- cellular signaling pathways, we established PC12 cell line overexpressing ... do not change levels of both proteins in rat's brain. However, since NCS-1 and DARPP-32 levels are not altered in wild type rats, we treated wild type PC12 cells (PC12 WT) and PC12 cells...
Ngày tải lên: 11/08/2014, 07:21
Báo cáo y học: " Perceived protein needs and measured protein intake in collegiate male athletes: an observational study" potx
... protein intake. Actual protein intake was com- parab le to perceived protein needs (p = 0.16) and to the Figure 2 Actual Protein Intake. The RDI, maximum beneficial level of protein intake, and ... Protein metabolism during intensive physical training in the young adult. Am J Clin Nutr 1975, 28:29-35. 12. Tarnopolsky MA, MacDougall JD, Atkinson SA: Influence of protein intake and training status on ... differed in terms of protein content. Actual protein intake was quantified using 3-day food records and nutrient analysis. Single sample t-tests were used to compare protein intake and perceived protein...
Ngày tải lên: 11/08/2014, 23:21
báo cáo khoa học: " Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers" ppt
Ngày tải lên: 12/08/2014, 05:20
Báo cáo y học: "Effects of dietary phytoestrogens on plasma testosterone and triiodothyronine (T3) levels in male goat kids" pdf
Ngày tải lên: 12/08/2014, 18:22
Báo cáo khoa học: "A CC and CXC chemokine levels in children with meningococcal sepsis accurately predict mortality and disease severity" pptx
Ngày tải lên: 12/08/2014, 23:21
Báo cáo y học: " Exhaled nitric oxide and urinary EPX levels in infants: a pilot study" potx
Ngày tải lên: 13/08/2014, 13:22
báo cáo hóa học: " The possible link between the elevated serum levels of neurokinin A and anti-ribosomal P protein antibodies in children with autism" pot
... proliferation, invasion into living cells and starting apoptosis, a defect in the synthesis of apolipoprotein B resulting in accumulation of lipids inside the cell, and downregulation of the total protein ... tried to find a possible link between the elevated serum levels of neurokinin A and anti-ribosomal P protein antibodies in autism. In this work, patients with elevated serum neurokinin A levels ... levels of neurokinin A and anti-ribosomal P protein antibodies was also studied. Methods: Serum neurokinin A and anti-ribosomal P protein antibodies were measured in 70 autistic children in...
Ngày tải lên: 19/06/2014, 22:20
Báo cáo y học: "Decreased levels of soluble amyloid β-protein precursor and β-amyloid protein in cerebrospinal fluid of patients with systemic lupus erythematosus" ppsx
... 1 Decreased levels of soluble amyloid β -protein precursor and β-amyloid protein but increases of intrathecal axonal degradation products and TGF-β in patients with cerebral lupus. (a) Cerebrospinal ... SLE and age-matched controls were evaluated clinically, with magnetic resonance imaging of the brain and CSF analyses. Levels of tau, amyloid precursor protein (APP), β-amyloid protein (Aβ42), and transforming ... without MRI-verified brain pathology. This finding, together with increased intrathecal levels of TGF-β, indicates an ongoing destructive parenchymatous process in the SLE brain. In addition, the present...
Ngày tải lên: 09/08/2014, 01:23
Báo cáo y học: "Osteogenic protein 1 in synovial fluid from patients with rheumatoid arthritis or osteoarthritis: relationship with disease and levels of hyaluronan and antigenic keratan sulfate" ppt
... the protein bands of interest. The same amount of protein was loaded onto each lane. Figure 3 Content of osteogenic protein 1 protein in synovial fluid samplesContent of osteogenic protein 1 protein ... The inclusion of other catabolic agents, such as proinflammatory cytokines and proteinases, in such analyses may provide a better under- standing of the processes occurring in the articular joint. Conclusion Taken ... the acquisition, analysis and interpretation of the data and in draft- ing the manuscript. MM was involved in sample acquisition and analysis of the data. BK was involved in the development and adaptation...
Ngày tải lên: 09/08/2014, 08:22
báo cáo khoa học: " The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism" potx
... steady-state levels were increased in gun2 (allele of hy1 - required for phytochromobilin synthesis from heme) [35], gun4 (mutant in the Proto- porphyrin I X- and Mg-Protoporphyrin IX-binding pro- tein) ... precursor proteins. Plant J 2001, 27:59-65. 66. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Analyt ... targeting det erminants for chloroplasts and mito- chondria are usually located at the N-terminus of the protein; therefore, a fusion protein with GFP fused to the C-terminus of TSPO was made. Using...
Ngày tải lên: 11/08/2014, 11:21