pro and anti apoptotic functions of ros
Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
... apoptosis I.1.b Apoptotic machinery I.2 Reactive oxygen species and apoptosis I.2.a Pro and Anti- Apoptotic functions of ROS I.2.b Rac1 and superoxide anion production 11 I.2.c Superoxide anion and inhibition ... via the Fenton reaction I.2.a Pro and Anti- Apoptotic functions of ROS Over the past decade or so there has been a paradigm shift in the understanding of ROS and the functions they can play in the ... Bid that is a pro- apoptotic member of Bcl-2 family Pro- and antiapoptotic proteins of this group form heterodimers and block each other’s activity Expression levels of these proteins can be controlled...
Ngày tải lên: 16/09/2015, 08:31
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... guard cells of broad beans involves protein kinase-mediated phosphorylation of Ser and Thr residues in the C-terminus of the pump and 14-3-3 binding [17] Much less is known about in vitro and in vivo ... does not resemble any protein of known function, but it is probably the first identified member of a new family of plant regulatory proteins, as at least five A thaliana genes and many expressed sequence ... Vmax and apparent Km values were, respectively, 1.20 ± 0.04 lmolÆmin)1Æmg protein)1 and 0.35 ± 0.05 mM in the absence and 1.37 ± 0.06 lmolÆmin)1Æmg protein)1 and 0.13 ± 0.02 mM in the presence of...
Ngày tải lên: 19/02/2014, 07:20
Fabrication of a porous polyimide membrane using a silicon nanowire array as a template
... hexagonal cross sections of the nanowires were transferred to the pores (Fig 4d) Further optimization of the described process is expected to increase the ranges of the pore diameters and densities ... nanowires with various diameters of ~50, 90, 150 and 220 nm synthesized from Au nanoparticles with diameter of ~ 30, 50 100 and 150 nm, respectively Fig a, b, and c show polyimide membranes fabricated ... with O2 plasma, and (d) silicon etching with XeF2 A schematic of a cross sectional view is shown under each SEM image Fig SEM images of porous polyimide membranes with pore density of (a, b) ~ pore/100...
Ngày tải lên: 16/03/2014, 15:10
SCAFFOLDING AN EFL (ENGLISH AS A FOREIGN LANGUAGE) ''''EFFECTIVE WRITING'''' CLASS IN A HYBRID LEARNING COMMUNITY pot
... formation of an education community of practice, “providing opportunities and mechanisms for teachers of all levels to overcome their isolation and make more effective use of time spent on professional ... heartfelt and sincere gratitude to Professor Kar-Tin Lee and Associate Professor Rod Nason for their encouragement, patience and invaluable guidance Their kind, constant and thoughtful assistance meant ... in the K-12 environments as well and, as a result, online course offerings spanning all kinds of disciplines (Palloff & Pratt, 2007a) and across various sectors of society (Lock, 2007) are being...
Ngày tải lên: 19/03/2014, 07:20
Báo cáo khoa học: Identification of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx
... permits the formation of multiprotein complexes, stable subcellular localizations of interacting partners and the coordination of their activities MAGUK contain a number of protein–protein interaction ... PDZ2 domain of hDlg and the C-terminal RTAV sequence of MEK2 are required for the optimal interaction of the two protein partners Coimmunoprecipitation of endogenous hDlg and MEK2 proteins To ... Materials and methods Antibodies The antibodies used were: anti- Dlg (sc-9961) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti- MEK2 (ab32517), anti- MEK1 (ab32091), anti- Raf1 (ab18761), anticalreticulin...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
... 16085204) from the Ministry of Education, Sports, Culture, Science, PROBRAIN, and Technology of Japan, and a grant from the Global Research Programme of the Ministry of Science and Technology, South ... positions of PCaP1 (36 kDa) and CBL4 (27 kDa) Translation products were detected by immunoblotting with anti- PCaP1 (middle panel) and anti- (His)6 (right panel) IgG Molecular masses (kDa) of the standard ... component and accounts for 0.079% and 0.029% of the total membrane protein from shoots and roots, respectively, of A thaliana seedlings (Fig 2) The aim of this study was to clarify the mechanism of...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
... MgCl2, 25 mM sucrose, and 0.1 mM EDTA (pH 7.4) Fluorescences of BIPM (A and B) and FITC (C and D) were measured with K+-activated phosphatase (A and C) and Na+-dependent ATPase (B and D) under pressures ... expected to alter the environment of transmembrane proteins in the contact surfaces of proteins and water, and/ or protein and lipid bilayer: an increase in the thickness of the lipid bilayer partially ... pressure of 100± 220 MPa causes dissociation of a and b subunits, disassembly of transmembrane a helices, and a separation in the contact surface of membrane and protein due to the thickening and...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles A possible role in membrane secretion pptx
... blocked and then sequentially incubated with the rabbit anti- (GST–MFG-E8) serum and peroxidase-conjugated goat anti- (rabbit IgG) Ig The protein bands probed with the peroxidase-labeled antibody ... elution pro les of MFG-E8 and its mutants were monitored by ELISA ELISA plate was coated directly with each fraction, and the antigens were detected by using the rabbit anti( GST–MFG-E8) serum and ... localization of MFG-E8 depending on the C-domains COS-7 cells were transfected with plasmids containing MFG-E8-L (A and B), MFG-E8-S (C and D), DC1 (E and F) and DC2 (G and H) or empty plasmid (I and...
Ngày tải lên: 24/03/2014, 03:21
Cell Membrane: The Red Blood Cell as a Model ppt
... Ankyrin 117 Functions of Ankyrin 117 Erythroid and Nonerythroid Ankyrins 118 Protein 4.2 118 Protein Chemistry of Protein 4.2 118 Functions of Protein 4.2 120 Binding Properties of Protein 4.2 ... the Cytoplasmic Domain of Band 84 Anion Exchange Channel by the Transmembrane Domain of Band 84 Lateral and Rotational Mobility of Band 85 Blood Type Antigens and Band 85 Band in Nonerythyroid ... that is: band (a-spectrin), band (b-spectrin), band 2.1 (ankyrin), band (anion exchanger 1: AEÀ1), band 4.1 (protein 4.1), band 4.2 (protein 4.2), band (actin), band (gly- 1.4 Commencement of Membrane...
Ngày tải lên: 29/06/2014, 11:20
Báo cáo y học: "Plasma gelsolin as a biomarker of inflammation" doc
... bactericidal activity of cationic endogenous antimicrobial peptides and exogenous antibiotics In fact, gelsolin was first investigated as an inhaled therapeutic agent to decrease the viscosity of airway ... patients with cystic fibrosis Appropriately dosed infusions of recombinant human gelsolin can diminish evolving injury or reduce mortality rates in animal models of hyperoxia, burns, and sepsis [9-11] ... of the initial insult and subsequent complications in patients identified by marked hypogelsolinemia as being at substantial risk for delayed multiorgan dysfunction could provide a directed and...
Ngày tải lên: 09/08/2014, 13:22
báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc
... ZmPti1a5protein expression and kinase activity ZmPti1a protein expression and kinase activity (A) Immunodetection of ZmPti1a protein in various tissues at different developmental stages of maize Proteins ... molecular size of the ZmPti1a protein of approximately 41 kDa is visible in extracts from mature pollen Faint bands could be detected in protein of staminate spikelets at anthesis (0 dba) and pollinated ... EcoRI PCR product (c2i) of the c2 gene [56] which contained 16 bp of the 3' border of exon 1, the entire intron 1, and 39 bp of the 5' border of exon was cloned into the EcoRI site of pBluescriptKS-...
Ngày tải lên: 12/08/2014, 05:20
Báo cáo y học: "Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients" pptx
... optimal cutoff value for plasma DNA to predict ICU mortality and hospital mortality was 127 ng/ml This cutoff value gave a sensitivity of 92% and a specificity of 80% for ICU mortality and a sensitivity ... significance (data not shown) Prognostic ability of plasma DNA ROC curves were calculated for the use of plasma DNA as a predictor of either ICU death or hospital death and for the SOFA score to predict ... predictive of death with a sensitivity and a specificity of 100% and 81%, respectively [12] A prognostic value has also been shown in patients with acute myocardial infarction [11] and acute stroke...
Ngày tải lên: 12/08/2014, 23:23
Báo cáo y học: "Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines" pot
... of IL-12 in the stimulation of IFN-g production, proliferation of T cells, and generation of cytotoxic T lymphocytes [23], using LMP1 as an adjuvant should result in increased DC activation and ... dilution of mouse anti- CD40 polyclonal antibody (C-20, Santa Cruz Biotechnology, Santa Cruz, CA), and (iii) 1:2,000 dilution of mouse anti- Gag p27 antibody, obtained through the National Institutes of ... staining with anti- CD40, anti- CD80, anti- CD83, antiCD86, anti- CD11c or anti- HLA-DR or anti- CCR7 in fluorescence-activated cell sorter buffer (PBS supplemented with 3% fetal calf serum and 0.02% sodium...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: "A mouse plasma peptide atlas as a resource for disease proteomics" ppt
... range of prostate specific antigen Detection of low abundance proteins results from a combination of extensive fractionation of intact proteins using an orthogonal two-dimensional system of AX and ... sample processing protocols, other database search algorithms and analysis of disease models The identified proteins span an estimated logarithm range of abundance encompassing prostate specific antigen, ... to characterize splice isoform products When the protein products of alternative splice isoforms differ in structure, we expect that the physical properties of these proteins, in particular their...
Ngày tải lên: 14/08/2014, 08:21
Development of chick chorioallantoic membrane as a biological testing membrane
... ACKNOWLEDGEMENTS My deepest gratitude and sincere appreciation to my supervisors, Assoc Prof Chan Lai Wah and Assoc Prof Paul Heng Prof Chan has been the epitome of dedication and excellence in her steadfast ... of (a) haemorrhage and (b) embryotoxicity .80 Figure 20 Appearance of CAM (a) after the application of 30 mg/kg of propranolol and (b) after application of 7.5 mg/kg of propranolol 80 Figure ... indicate the rate and amount of drug absorption Another approach of assessing absorption involves the evaluation of the permeation profile of the drug through the CAM Table Comparison of composition...
Ngày tải lên: 11/09/2015, 10:00
Evaluation of poly (ethylene glycol) grafting as a tool for improving membrane performance
... Isometric view of AFM of unmodified membrane 74 5.18B Isometric view of AFM of membrane modified by bulk approach 74 5.18C Isometric view of AFM of membrane modified by drop wise approach 75 5.19 ... irradiation of PET in the presence of bi-functional media resulted in an increase of surface modulus, lower expansion, and lower soft/melt temperatures indicating surface crosslinking and a decrease of ... higher production rates can be achieved 1.2 Modes of Filtration Water purification is a rigorous process which requires removal of a large number of impurities of varying size, shape and solubility...
Ngày tải lên: 05/07/2016, 14:26
Lab Linux phan I, II Installing Linux as a Server
... www.athena.edu.vn Chọn nhóm software nên trái chọn nhóm software bên phải chọn Optional packages hiển thị danh sách software Thực chọn|bỏ chọn để cài đặt hay gỡ bỏ software Chọn Close chọn ... www.athena.edu.vn Chọn nhóm software nên trái chọn nhóm software bên phải Chọn Optional packages hiển thị danh sách software Thực chọn|bỏ chọn để cài đặt hay gỡ bỏ software Biên soạn: Nguyễn Trí ... Management Tool (dùng giao diện đồ họa); - Vào menu Applications chọn Add/Remove Software (nếu bạn dùng command line có thề dùng lệnh : system-config-packages) Màn hình Package Manager Biên soạn:...
Ngày tải lên: 13/09/2012, 10:21
Báo cáo y học: " Self-reported sickness absence as a risk marker of future disability pension. Prospective findings from the DWECS/DREAM study 1990-2004"
... conducted in 1990, and featured a random sample drawn from the Central Population Register of Denmark of 9653 people aged 18-59 Of these, 8664 participated in the survey (response rate 90%) Of these, ... decades: An upper cut-off point of 45 years of ages was chosen to ensure a study population considerably younger than the official retirement age, and to ensure a maximum age of 59 during follow-up: ... multi-register based and prospective study Scand J Public Health 2003;31:246-54 Szubert Z, Sobala W Current determinants of early retirement among blue collar workers in Poland Int J Occup Environ...
Ngày tải lên: 26/10/2012, 10:03
Báo cáo y học: "An Avian Connection as a Catalyst to the 1918-1919 Influenza Pandemic"
... layers of viral enveloping: one layer is composed of cholesterol, and the other layer of two proteins: hemagglutinin [HA] and nueraminidase [NA] Both proteins are recognized by antibodies The two proteins ... different H and different N antigens have been identified Type A influenza viruses are made up of various combinations of H and N antigens but only a few of these combinations: H1N1, H2N2, H3N2 and H5N1 ... believed to be the source of many of the influenza viruses and are responsible for their reemergence prior to pandemics [8, 12, 20] With the difficulties of antigenic shift, drift, and animal reservoirs,...
Ngày tải lên: 02/11/2012, 11:12