pro and anti apoptotic functions of ros 7
Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
... apoptosis I.1.b Apoptotic machinery I.2 Reactive oxygen species and apoptosis I.2.a Pro and Anti- Apoptotic functions of ROS I.2.b Rac1 and superoxide anion production 11 I.2.c Superoxide anion and inhibition ... via the Fenton reaction I.2.a Pro and Anti- Apoptotic functions of ROS Over the past decade or so there has been a paradigm shift in the understanding of ROS and the functions they can play in the ... Bid that is a pro- apoptotic member of Bcl-2 family Pro- and antiapoptotic proteins of this group form heterodimers and block each other’s activity Expression levels of these proteins can be controlled...
Ngày tải lên: 16/09/2015, 08:31
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... Binding of 14-3-3 protein to the plasma membrane H+-ATPase AHA2 involves the three C-terminal residues Tyr946-Thr-Val and requires phosphorylation of Thr9 47 J Biol Chem 274 , 3 677 4–3 678 0 5 870 C Viotti ... (AHA1 isoform): site and mechanism of action on H+-ATPase activity differ from those of 14-3-3 proteins Plant J 31, 4 87 4 97 De Michelis MI, Papini R & Pugliarello MC (19 97) Multiple effects of lysophosphatidylcholine ... guard cells of broad beans involves protein kinase-mediated phosphorylation of Ser and Thr residues in the C-terminus of the pump and 14-3-3 binding [ 17] Much less is known about in vitro and in vivo...
Ngày tải lên: 19/02/2014, 07:20
Fabrication of a porous polyimide membrane using a silicon nanowire array as a template
... hexagonal cross sections of the nanowires were transferred to the pores (Fig 4d) Further optimization of the described process is expected to increase the ranges of the pore diameters and densities ... nanowires with various diameters of ~50, 90, 150 and 220 nm synthesized from Au nanoparticles with diameter of ~ 30, 50 100 and 150 nm, respectively Fig a, b, and c show polyimide membranes fabricated ... with O2 plasma, and (d) silicon etching with XeF2 A schematic of a cross sectional view is shown under each SEM image Fig SEM images of porous polyimide membranes with pore density of (a, b) ~ pore/100...
Ngày tải lên: 16/03/2014, 15:10
SCAFFOLDING AN EFL (ENGLISH AS A FOREIGN LANGUAGE) ''''EFFECTIVE WRITING'''' CLASS IN A HYBRID LEARNING COMMUNITY pot
... 72 Table 3.2 Strengths of Data Collection Methods 76 Table 3.3 Summary of the Data Collection and Schedule 76 Table 3.4 Action Research Cycle, Phases, and Steps 77 Table ... environments as well and, as a result, online course offerings spanning all kinds of disciplines (Palloff & Pratt, 2007a) and across various sectors of society (Lock, 20 07) are being developed ... formation of an education community of practice, “providing opportunities and mechanisms for teachers of all levels to overcome their isolation and make more effective use of time spent on professional...
Ngày tải lên: 19/03/2014, 07:20
Báo cáo khoa học: Identification of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx
... Materials and methods Antibodies The antibodies used were: anti- Dlg (sc-9961) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti- MEK2 (ab325 17) , anti- MEK1 (ab32091), anti- Raf1 (ab1 876 1), anticalreticulin ... PDZ2 domain of hDlg and the C-terminal RTAV sequence of MEK2 are required for the optimal interaction of the two protein partners Coimmunoprecipitation of endogenous hDlg and MEK2 proteins To ... permits the formation of multiprotein complexes, stable subcellular localizations of interacting partners and the coordination of their activities MAGUK contain a number of protein–protein interaction...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
... sequence of PCaP1 (PCaP11) 27) and the other with a modified N-terminal 27- residue sequence, in which Gly2 was replaced by Ala (PCaP11) 27 ⁄ G2A) and Green fluorescence of PCaP11) 27- GFP 1) 27 ⁄ G2A ... positions of PCaP1 (36 kDa) and CBL4 ( 27 kDa) Translation products were detected by immunoblotting with anti- PCaP1 (middle panel) and anti- (His)6 (right panel) IgG Molecular masses (kDa) of the standard ... component and accounts for 0. 079 % and 0.029% of the total membrane protein from shoots and roots, respectively, of A thaliana seedlings (Fig 2) The aim of this study was to clarify the mechanism of...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
... MgCl2, 25 mM sucrose, and 0.1 mM EDTA (pH 7. 4) Fluorescences of BIPM (A and B) and FITC (C and D) were measured with K+-activated phosphatase (A and C) and Na+-dependent ATPase (B and D) under pressures ... lL of mM soy-PtdCho liposome (see below), 100 lL of tritiated water (3 .7 MBqámL)1), and 40 lL of 40 mM Tris/HCl buffer, pH 7. 5, were mixed and pressurized at 37 °C for 15 A 200-lL aliquot of ... expected to alter the environment of transmembrane proteins in the contact surfaces of proteins and water, and/ or protein and lipid bilayer: an increase in the thickness of the lipid bilayer partially...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles A possible role in membrane secretion pptx
... blocked and then sequentially incubated with the rabbit anti- (GST–MFG-E8) serum and peroxidase-conjugated goat anti- (rabbit IgG) Ig The protein bands probed with the peroxidase-labeled antibody ... containing 150 mM NaCl and mM EDTA], and placed in centrifuge tubes The mixtures were layered successively with mL of 60% (w/v), mL of 30% (w/v) and mL of 5% (w/v) sucrose in buffer A, and centrifuged ... localization of MFG-E8 depending on the C-domains COS -7 cells were transfected with plasmids containing MFG-E8-L (A and B), MFG-E8-S (C and D), DC1 (E and F) and DC2 (G and H) or empty plasmid (I and...
Ngày tải lên: 24/03/2014, 03:21
Cell Membrane: The Red Blood Cell as a Model ppt
... Nonerythroid Protein 4.1 Isoforms 69 Actin 71 Other Minor Skeletal Proteins 72 The p55 Protein 72 Adducin 72 Dematin (Protein 4.9) 73 Tropomyosin 74 Tropomodulin 74 Other Membrane Proteins 74 Structure of ... Ankyrin 1 17 Functions of Ankyrin 1 17 Erythroid and Nonerythroid Ankyrins 118 Protein 4.2 118 Protein Chemistry of Protein 4.2 118 Functions of Protein 4.2 120 Binding Properties of Protein 4.2 ... Gene 126 Protein 4.2 Gene in Mouse Red Cells 1 27 Tissue-Specific Expression of the Mouse Protein 4.2 Gene and the Pallid Mutation 1 27 VII VIII Contents 7. 1 7. 2 7. 2.1 7. 2.2 7. 2.3 7. 3 7. 3.1 7. 3.2...
Ngày tải lên: 29/06/2014, 11:20
Báo cáo y học: "Plasma gelsolin as a biomarker of inflammation" doc
... bactericidal activity of cationic endogenous antimicrobial peptides and exogenous antibiotics In fact, gelsolin was first investigated as an inhaled therapeutic agent to decrease the viscosity of airway ... patients with cystic fibrosis Appropriately dosed infusions of recombinant human gelsolin can diminish evolving injury or reduce mortality rates in animal models of hyperoxia, burns, and sepsis [9-11] ... of the initial insult and subsequent complications in patients identified by marked hypogelsolinemia as being at substantial risk for delayed multiorgan dysfunction could provide a directed and...
Ngày tải lên: 09/08/2014, 13:22
báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc
... ZmPti1a5protein expression and kinase activity ZmPti1a protein expression and kinase activity (A) Immunodetection of ZmPti1a protein in various tissues at different developmental stages of maize Proteins ... molecular size of the ZmPti1a protein of approximately 41 kDa is visible in extracts from mature pollen Faint bands could be detected in protein of staminate spikelets at anthesis (0 dba) and pollinated ... cells as a Ca2+-dependent process Plant Physiol 1985, 77 :544-551 Resh MD: Fatty acylation of proteins: new insights into membrane targeting of myristoylated and palmitoylated proteins Biochim Biophys...
Ngày tải lên: 12/08/2014, 05:20
Báo cáo y học: "Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients" pptx
... behalf of the Working Group on Sepsis-Related Problems of the European Society of Intensive Care Medicine Intensive Care Med 1996, 22 :70 7 -71 0 18 Bone RC: Let's agree on terminology: definitions of ... interval, 0 .71 –0. 97) and that for the SOFA score is 0 .76 (95% confidence interval, 0.61–0.92) attempting to determine a 'normal reference range' in Taiwanese medical students revealed a mean of 57. 1 ... 0 .76 (95% confidence interval, 0.61–0.92) The optimal cutoff value for plasma DNA to predict ICU mortality and hospital mortality was 1 27 ng/ml This cutoff value gave a sensitivity of 92% and...
Ngày tải lên: 12/08/2014, 23:23
Báo cáo y học: "Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines" pot
... cells and staining with anti- CD40, anti- CD80, anti- CD83, antiCD86, anti- CD11c or anti- HLA-DR or anti- CCR7 in fluorescence-activated cell sorter buffer (PBS supplemented with 3% fetal calf serum and ... Biotechnology, Santa Cruz, CA), and (iii) 1:2,000 dilution of mouse anti- Gag p 27 antibody, obtained through the National Institutes of Health AIDS Research and Reference Reagent Program (Germantown, MD) ... of IL-12 in the stimulation of IFN-g production, proliferation of T cells, and generation of cytotoxic T lymphocytes [23], using LMP1 as an adjuvant should result in increased DC activation and...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: "A mouse plasma peptide atlas as a resource for disease proteomics" ppt
... assembly Of the set of 13 ,77 9 distinct peptides, 9, 170 ( 67% ) were identified with at least spectra Cys-containing, Trp-containing, and Lys-containing peptides represented 3 ,70 9 ( 27% ), 2,0 67 (15%), and ... range of prostate specific antigen Detection of low abundance proteins results from a combination of extensive fractionation of intact proteins using an orthogonal two-dimensional system of AX and ... sample processing protocols, other database search algorithms and analysis of disease models The identified proteins span an estimated logarithm range of abundance encompassing prostate specific antigen,...
Ngày tải lên: 14/08/2014, 08:21
Development of chick chorioallantoic membrane as a biological testing membrane
... ACKNOWLEDGEMENTS My deepest gratitude and sincere appreciation to my supervisors, Assoc Prof Chan Lai Wah and Assoc Prof Paul Heng Prof Chan has been the epitome of dedication and excellence in her steadfast ... of (a) haemorrhage and (b) embryotoxicity .80 Figure 20 Appearance of CAM (a) after the application of 30 mg/kg of propranolol and (b) after application of 7. 5 mg/kg of propranolol 80 Figure ... Doppler head and sample 73 Figure 16 Relationship between amplitude and blood perfusion of CAM (EA 9) .74 Figure 17 Relationship between threshold and blood perfusion of CAM (EA 9) 75 Figure...
Ngày tải lên: 11/09/2015, 10:00
Evaluation of poly (ethylene glycol) grafting as a tool for improving membrane performance
... Summary and Discussion of Results Conclusions and Recommendations 105 1 07 7.1 Background 1 07 7.2 Salient Features of Method I 108 7. 3 Salient Features of Method II 110 7. 4 Comparison of Method I and ... 73 5.18A Isometric view of AFM of unmodified membrane 74 5.18B Isometric view of AFM of membrane modified by bulk approach 74 5.18C Isometric view of AFM of membrane modified by drop wise approach ... polymerization process 70 xii 5.15 Functioning of CTA in polymerization 70 5.16 Comparison of flux between unmodified and modified membranes 72 5. 17 Comparison of TOC rejection between unmodified and modified...
Ngày tải lên: 05/07/2016, 14:26
Lab Linux phan I, II Installing Linux as a Server
... www.athena.edu.vn Chọn nhóm software nên trái chọn nhóm software bên phải chọn Optional packages hiển thị danh sách software Thực chọn|bỏ chọn để cài đặt hay gỡ bỏ software Chọn Close chọn ... www.athena.edu.vn Chọn nhóm software nên trái chọn nhóm software bên phải Chọn Optional packages hiển thị danh sách software Thực chọn|bỏ chọn để cài đặt hay gỡ bỏ software Biên soạn: Nguyễn Trí ... – 0989012418 www.athena.edu.vn 7/ Hướng dẫn cài đặt phần mềm Unikey: - Cài đặt gói rpm: rpm –ivh x-unikey-0.9.2-fc3-1.i386.rpm - Thêm dòng sau vào file /etc/profile export LANG=en_US.UTF-8 export...
Ngày tải lên: 13/09/2012, 10:21
Báo cáo y học: " Self-reported sickness absence as a risk marker of future disability pension. Prospective findings from the DWECS/DREAM study 1990-2004"
... 89 14 37 30 98 45 95 45 95 33 1 07 34 106 33 1 07 41 99 29 111 38 102 36 104 Model I 95% CI 1 .77 -4.33 0.96-2.61 0.59-2.05 P 0.00 0. 07 0 .77 0.92-1.98 0.12 3.02 -7. 88 1.28-3.45 0.00 0.00 1 .72 -9.93 ... N 1026 980 71 9 1449 2003 2 171 1024 1596 1554 940 439 1 472 78 9 492 1925 686 1563 151 159 838 30 07 1040 3134 1003 3 171 1034 3140 860 3314 1029 3145 655 3518 651 3518 75 6 3413 70 1 3 470 Cases 58 ... 1.20-8 .73 1.30 -7. 30 0 .76 -4.90 OR 2 .77 1.58 1.10 1.00 1.35 1.00 4.88 2.10 1.00 4.13 3.24 3.08 1.93 1.00 0.00 0.02 0.01 0. 17 OR 2.68 1.58 1.14 1.00 1.42 1.00 5.05 2.13 1.00 3 .74 3.13 2.95 1 .74 1.00...
Ngày tải lên: 26/10/2012, 10:03
Báo cáo y học: "An Avian Connection as a Catalyst to the 1918-1919 Influenza Pandemic"
... layers of viral enveloping: one layer is composed of cholesterol, and the other layer of two proteins: hemagglutinin [HA] and nueraminidase [NA] Both proteins are recognized by antibodies The two proteins ... the CrossSpecies Transfer and Evolution of the SARS Coronal Virus Philos Trans R Soc Lond B Biol Sci 2004; 359(14 47) :10 67- 73 17 Scholtissek C Pigs as ‘Mixing Vessels’ for the Creation of New Pandemic ... different H and different N antigens have been identified Type A influenza viruses are made up of various combinations of H and N antigens but only a few of these combinations: H1N1, H2N2, H3N2 and H5N1...
Ngày tải lên: 02/11/2012, 11:12