principles of electrophysiological in vitro measurements
Tài liệu Principles of development in biology docx
Ngày tải lên :
16/02/2014, 21:20
... bird wing developed independently from the bat wing Bats descended from a long line of nonwinged mammals, and the structure of the bat wing is markedly different from that of a bird wing One of ... involves the creation of new individuals Sex involves the combining of genes from two different individuals into new arrangements Reproduction in the absence of sex is characteristic of organisms that ... areas in which such mathematical modeling has given biologists interesting insights into some underlying laws of animal development The mathematics of organismal growth Most animals grow by increasing...
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Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc
Ngày tải lên :
07/03/2014, 15:20
... combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of ... blocking of certain nitrosation sites Surprisingly, we did not observe any changes in the TOCSY NMR spectra upon the addition of GSH (data not shown), which is indicative of no specific binding ... cytotoxic and DNA-damaging effects of nitric oxide [29] So, while the binding of NO to GSH in vivo does not obviously prevent NO from interacting with MT2, we have shown that in vitro it suppresses...
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Báo cáo hóa học: " The effect of acyclovir on the tubular secretion of creatinine in vitro" pdf
Ngày tải lên :
18/06/2014, 16:20
... concentration of [2-14C] creatinine in the apical compartment in the absence of quinidine Creatinine transport was significantly (p < 0.05) inhibited in the presence of quinidine at 30 minutes The ... creatinine in the presence or absence of acyclovir (22 to 89 μM) In contrast, there was a decrease in the concentration of [2-14C] creatinine in the apical compartment in the presence of quinidine ... Creatinine continued to increase following acyclovir discontinuation Creatinine returned to normal within week Elevated urea Mild proteinuria High urea Urinary a1-microglobulin and albumin Creatinine...
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Báo cáo sinh học: " Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" pptx
Ngày tải lên :
19/06/2014, 08:20
... reaction Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for ... Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course of I7L-mediated ... detection of protein In vitro cleavage assay Reactions were performed at the indicated temperature in a final volume of 20 µL containing µL of substrate, 13 µL of enzyme extract, and µL of 20 mM...
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báo cáo hóa học:" Development of an in vitro three dimensional loading-measurement system for long bone fixation under multiple loading conditions: a technical description" potx
Ngày tải lên :
20/06/2014, 01:20
... cortical bone longitudinal modulus of elasticity increases with increase in strain rate by a factor of 1.5 for strain rate of 1500 compared to 1.0 (1/sec), thus explaining in part the greater stiffness ... time in storage], test loading [strain] rate, relative stiffness of the potting-fixtures-testing machine [Kpft] to actual stiffness of the specimen [Ksa], fixture constraints Figure 6of the A) intact ... potting of the ends could be used to test a specimen in all three modes of loading: axial compression, torsion and bending In addition the bending fixtures were capable of applying uniform 4-point...
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báo cáo hóa học:" Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" docx
Ngày tải lên :
20/06/2014, 04:20
... reaction Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for ... Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course of I7L-mediated ... detection of protein In vitro cleavage assay Reactions were performed at the indicated temperature in a final volume of 20 µL containing µL of substrate, 13 µL of enzyme extract, and µL of 20 mM...
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Báo cáo toán học: " Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" doc
Ngày tải lên :
20/06/2014, 20:20
... Dulbecco's minimum essential medium [DMEM]: Ham's F-12 media containing 10% heat inactivated foetal bovine serum [FBS], mM L-glutamine and 1% penicillin streptomycin [Penstrep] (all purchased from Invitrogen ... a number of factors including an increase in the coating diameter upon addition of the PEG moiety [33], thus increasing the stability of the nanoparticles and further shielding the cells from ... [1] In the field of tissue engineering, magnetic nanoparticles have been previously reported for many applications including cellular labelling, sorting and monitoring, targeted in- vivo therapeutic...
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báo cáo hóa học:" Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" potx
Ngày tải lên :
21/06/2014, 17:20
... a number of factors including an increase in the coating diameter upon addition of the PEG moiety [33], thus increasing the stability of the nanoparticles and further shielding the cells from ... ability of various assays in discriminating the subtle difference in the level of effect those nanoparticles could impose to cells Another consideration in assessing the sensitivity of the cell ... Lglutamine and 1% penicillin streptomycin [Penstrep] (all purchased from Invitrogen Ltd., Renfrew, UK) RAW264.7 cells (kindly donated by Prof Colin Watts and Dr Alan Prescott, College of Life...
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PRINCIPLES OF CONDUCT IN ELECTRONIC COMMERCE pdf
Ngày tải lên :
28/06/2014, 12:20
... In our electronic commerce activities, we shall be guided by the following principles Scope of the Electronic Commerce Principles These Principles apply to all our Electronic Commerce These Principles ... Principles apply in their entirety General principles of Electronic Commerce The aim of these Principles is to promote trust in Electronic Commerce and therefore, we subscribe to the following: ... information regarding certification and/or authorisation of services/products; and information regarding the agency from which such qualification has been obtained; § the principal features of...
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January 2001 PRINCIPLES OF CONDUCT IN ELECTRONIC COMMERCE ppt
Ngày tải lên :
28/06/2014, 18:20
... In our electronic commerce activities, we shall be guided by the following principles Scope of the Electronic Commerce Principles These Principles apply to all our Electronic Commerce These Principles ... Principles apply in their entirety General principles of Electronic Commerce The aim of these Principles is to promote trust in Electronic Commerce and therefore, we subscribe to the following: ... information regarding certification and/or authorisation of services/products; and information regarding the agency from which such qualification has been obtained; § the principal features of...
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principles of epidemiology in public health practice|_part2 pot
Ngày tải lên :
12/08/2014, 06:20
... breathing problems and shock Inhalation anthrax is often fatal Eleven of the mail-related cases were inhalation; (45%) of the 11 patients died Intestinal: Initial signs of nausea, loss of appetite, ... appetite, vomiting, and fever are followed by abdominal pain, vomiting of blood, and severe diarrhea Intestinal anthrax results in death in 25% to 60% of cases While most human cases of anthrax result ... on the skin after handling infected livestock or contaminated animal products Skin infection begins as a raised itchy bump that resembles an insect bite but within 1-2 days develops into a vesicle...
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principles of epidemiology in public health practice|_part3 pptx
Ngày tải lên :
12/08/2014, 06:20
... following? A Eliminating the reservoir B Eliminating the vector C Eliminating the host D Interrupting mode of transmission E Reducing host susceptibility 24 Which term best describes the pattern of ... Summarizing Data Page 2-37 EXAMPLE: Finding the Interquartile Range Find the interquartile range for the length of stay data in Table 2.8 on page 2-17 Step Arrange the observations in increasing ... www.adultpdf.com Summarizing Data Page 2-26 The midrange (midpoint of an interval) Definition of midrange The midrange is the half-way point or the midpoint of a set of observations The midrange...
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Báo cáo y học: "Attenuation of fibrosis in vitro and in vivo with SPARC siRNA" ppsx
Ngày tải lên :
12/08/2014, 12:20
... fibrosis in skin and reduced inflammation in lungs induced by bleomycin HE stains of mouse skin tissues (Figure 3-1) showed that four-week injections of bleomycin induced significant fibrosis in skin ... reduction of collagen in the lung tissues of bleomycin-induced mice (Figure 4-2) In bleomycin-induced mice, collagen content of lung tissues was 3.6-fold higher than that in salineinjected control ... herein were the very popular bleomycin-induced skin and pulmonary fibrosis in mice Subcutaneous injection and intratracheal instillation of siRNAs were used for tissue-specific treatments of skin...
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Báo cáo y học: " Cyclic hydrostatic pressure and cotton particles stimulate synthesis by human lung macrophages of cytokines in vitro" ppsx
Ngày tải lên :
12/08/2014, 14:20
... Poly(I:C) induces a dose dependent influx of inflammatory cells into the airways of mice Poly(I:C) induces a dose dependent influx of inflammatory cells into the airways of mice Mice were administered ... of mouse tracheas to poly(I:C) in vitro increases the expression of bradykinin B1 and B2 receptors on the smooth muscle and confers AHR to bradykinin [25] Notably, inhibition of the bradykinin ... acetylcholineinduced AHR following allergen sensitization and challenge [26] In contrast, AHR to histamine following parainfluenza-3 infection in guinea pigs was inhibited by a bradykinin B2 receptor...
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Báo cáo y học: " Cyclic hydrostatic pressure and cotton particles stimulate synthesis by human lung macrophages of cytokines in vitro" pptx
Ngày tải lên :
12/08/2014, 14:20
... property of adherence Cells were incubated in 20% Dulbecco's modified Eagles medium (DMEM, Invitrogen UK) + 1% Glutamine + 1% Penicillin/Streptomycin (Invitrogen UK) for hour at 37°C in 5% CO2 ... significant increases in cytokine synthesis in all three types of macrophage In addition, there appears to be a There was considerable variation in the amount by which secretion of an individual cytokine ... Ltd., UK, 0.3% in 100% methanol) for minutes before immersing in Giemsa solution (Raymond Lamb ltd., UK, 1% in pH 6.4 buffer) for a further 20 minutes Cytospins were then rinsed in pH 6.4 buffer,...
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Báo cáo y học: "Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection" potx
Ngày tải lên :
12/08/2014, 14:22
... determined in the current study, in the joint space With respect to the role of Il-1 signalling in OA, in the one human clinical study using recombinant IL-1ra as a treatment for OA, a single injection ... IL-1ra: interleukin-1 receptor antagonist; IL-1β: interleukin-1 beta; IL-4: interleukin-4; IL-6: interleukin-6; IL-10: interleukin-10; IL-13: interleukin-13; MMP: matrix metalloproteinases; NO: ... in vitro, suggesting that, if any, in vivo effects of the TNFα in the injected ACS would have been indirect It is not clear to what extent the increased levels of IL-6, OSM and lower levels of...
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Báo cáo y học: "Response to: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection – authors’ reply" ppt
Ngày tải lên :
12/08/2014, 15:22
... WJA, Creemers LB: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection Arthritis ... ACL surgery-preliminary report Int Orthop 2009, 33:413-418 Yang KG, Saris DB, Verbout AJ, Creemers LB, Dhert WJ: The effect of synovial fluid from injured knee joints on in vitro chondrogenesis ... diffrences from asymptomatic subjects in systemic markers of inflammation suggesting differences in innate immunity Paper presented at: Osteoarthritis Research Society International 2010 World Congress...
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Báo cáo y học: "Lung fibroblasts from patients with emphysema show markers of senescence in vitro" pdf
Ngày tải lên :
12/08/2014, 16:20
... and participated in the clinical characterization of patients as well as in obtaining informed consent HM provided the funding of the study and participated in the preparation of the manuscript ... replicative ageing of human endothelial cells J Cell Sci 2000, 113(Pt 20):3613-3622 Severino J, Allen RG, Balin S, Balin A, Cristofalo VJ: Is beta-galactosidase staining a marker of senescence in vitro ... covered a number of genes of diverse pathways that could be altered in emphysema or cellular senescence LOXL2 seemed of interest as involved in cross-linking collagens and elastin [24]; it has...
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Morphological hormonal and genetical analyses of early in vitro flowering in dendrobium chao praya smile
Ngày tải lên :
11/09/2015, 10:06
... conditions Effects of coconut water and sucrose on flowering induction Sporad analysis of pollinia Germination of pollen grains in vitro In vitro pollination and seed production in culture Results ... juvenile phase in orchids, and to induce flowering in vitro in order to achieve flowering in a shorter period of time To date, flowering in vitro has been successfully induced in Cymbidium (Kostenyuk ... importance of cytokinins in flowering was mainly observed in field experiments involving foliar spray or injection of cytokinins (Sakai et al., 2000; Blanchard and Runkle, 2008) The objective of this...
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DEVELOPMENT OF HUMAN IN VITRO MODELS FOR PREDICTING ORGAN SPECIFIC TOXICITY
Ngày tải lên :
30/09/2015, 06:22
... for the development of in vitro models. 19 1.4 Role of inflammation in drug-induced nephrotoxicity in humans In the development of in vitro models for the prediction of drug-induced nephrotoxicity, ... HPTC-based in vitro model for nephrotoxicology developed here employed drug-induced increases in mRNA expression levels of the pro-inflammatory interleukins (IL)-6 and IL-8 as endpoint Pro-inflammatory ... validation of endpoints suitable for in vitro prediction of drug-induced nephrotoxicity in humans 57 4.3.1 Model design and identification of suitable endpoints for in vitro nephrotoxicology...
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