pressure exerted by a liquid depends on

... dissociation constant and P o is the total peptide concentration. The last term in the equation takes into account slight aggregation that may take place at higher protein concentrations. Data fitting ... (2005) RB1 gene mutation up-date, a meta-analysis based on 932 reported mutations available in a searchable data- base. BMC Genet 6, 53. 4 Burkhart DL & Sage J (2008) Cellular mechanisms of tumour ... initial and final anisotropy signals. For all reactions tested, full saturation was achieved at a 1 : 1 molar ratio of titrant, indicating a 1 : 1 stoichiometry, which validates the use of a 1...

Ngày tải lên: 22/03/2014, 21:20

Ngày tải lên: 13/08/2014, 10:20

Ngày tải lên: 13/08/2014, 20:21

... internal composition of each subcomplex in contrast to UV irradiation. In fact, it is well k nown that adaptation of cyanobacterial phycobilisomes to light by complementary chromatic adaptation ... with an electrospray ion source (ESI-MS). An advantage of this approach is that information can be collected on the initial events, which take place as this organism adapts to environmental ch anges. Ultracentrifugation ... separation allowed a quantitative estimation of the relative amount of each protein component from the area underlying each peak. This was facilitated by the fact that the biliproteins are strongly c onserved...

Ngày tải lên: 08/03/2014, 16:20

... example, and teaches abstract vocabulary through association of ideas. I.5.3. Vocabulary teaching according to the Communicative approach (CLT) Communicative Language Teaching (CLT) is an approach ... their application in translation passages from one language into the other. In this method, very little teaching is done in the target language. Instead, readings in the target language are translated ... vocabulary teaching and learning, especially in the context of VUC. I.5.1.Vocabulary teaching according to the Grammar-translation method: The grammar-translation method of foreign language...

Ngày tải lên: 29/01/2014, 00:23

... nif regulation in K. pneumoniae. K. pneumoniae strain UN4495 was used as the parental strain that carries nifLA and a nifKÂ-lacZÂ fusion on the chromosome and thus allows monitoring of NifA-mediated transcription ... used as standards. Membrane preparation Cytoplasmic and membrane fractions of K. pneumo- niae UN4495 and mutant derivatives were separated by several centrifugation steps as recently described by Klopprogge ... expression of nifLA or nifA from the tac promoter was induced by the addition of 10 l M isopropyl-b- D -thiogalactopyranoside (IPTG). b-Galactosidase assay NifA-mediated activation of transcription...

Ngày tải lên: 17/03/2014, 10:20

... Y., Kasai, Y., Inoue, A. , Baba, T. & Taira, K. (2002) Existence of efficient divalent metal ion- catalyzed and inefficient divalent metal ion-independent channels in reactions catalyzed by hammerhead ... evolution. EMBO J. 16, 3289–3302. 25. Ikawa, Y., Naito, D., Aono, N., Shiraishi, H. & Inoue, T. (1999) Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA–RNA interaction. ... to an equal volume of STOP solution, and immediately frozen at )70°C. Samples were separated on 8 M urea/5% polyacrylamide gels, followed by autoradiography. Computations To quantify RNA signals,...

Ngày tải lên: 23/03/2014, 12:20

... endo-b-galactosidase activity by HPLC The standard assay was carried out as follows. A reaction mixture (500 lL) containing an appropriate substrate and endo-b-galactosidase in 10 m M sodium acetate ... and tetrasaccharide b-glycosides 1–8 as substrates for endo-b-galactosidase by combining a glycos- idase-catalysed transglycosylation and a glycosyltrans- ferase-catalysed reaction. Endo-b-galactosidases ... pylori: analysis by matrix-assisted laser desorption/ionization mass spectrometry after degradation with endo-b-galactosidase and by fast atom bombardment mass spectrometry of permethylated undegraded...

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 03/04/2014, 18:42

... first analytical solution for the transmembrane potential in the organelle membrane induced by a time-varying magnetic field. We then analyzed factors that impact on the polarization of the organelle, ... 10 KHz, and a 1.3 mV increase at 200 KHz, respectively. The phase change caused by organelle removal was only Figure 2 Regional polarization of the cytoplasmic membra ne and the organelle membrane by ... Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, M5S 1A1 , Canada . 4 Department of Surgery, University of Toronto, Toronto, Ontario, M5S 1A1 , Canada. Authors’ contributions HY...

Ngày tải lên: 19/06/2014, 08:20

... Sendai, Japan Email: Hiroshi Oyamada - theta@qg7.so-net.ne.jp; Atsuhiko Iijima - a- iijima@med.niigata-u.ac.jp; Akira Tanaka - a- tanaka@sss.fukushima- u.ac.jp; Kazuhiko Ukai - ukai@waseda.jp; Haruo ... Haruo Toda - toda@med.niigata-u.ac.jp; Norihiro Sugita - sugita@yoshizawa.ecei.tohoku.ac.jp; Makoto Yoshizawa - yoshizawa@yoshizawa.ecei.tohoku.ac.jp; Takehiko Bando* - bando@adm.niigata-u.ac.jp * ... movies Hiroshi Oyamada 1 , Atsuhiko Iijima 1 , Akira Tanaka 2 , Kazuhiko Ukai 3 , Haruo Toda 1 , Norihiro Sugita 4 , Makoto Yoshizawa 4 and Takehiko Bando* 1 Address: 1 Division of Integrated Physiology,...

Ngày tải lên: 19/06/2014, 10:20

... light shading] are shown along with the steady state band for each measure. Time till steady state was used as a meas- ure of the adaptation rate. Differences in day 1 versus day 2 adaptation rates ... data was sampled at 120 Hz. All other signals were sampled at 1200 Hz. Three-dimensional kinematic data was recorded using a 6-camera video system (Motion Analysis Corporation, Santa Rosa, CA) ... 0HGLDO *DVWURFQHPLXV /DWHUDO *DVWURFQHPLXV 6WHDG\6WDWH (0*506 1RUPDOL]HG )RRWVZLWFKFRQWURO 3URSRUWLRQDOP\RHOHFWULF FRQWURO  Ÿ Ÿ Ÿ Adaptation ratesFigure 7 Adaptation rates. Adaptation rates expressed as time in minutes to steady state for ankle angle correlation common variance, soleus EMG RMS,...

Ngày tải lên: 19/06/2014, 10:20

... via the anaphylatoxins/anaphylatoxin receptors and was not due to contaminants in anaphyla- toxin preparations. Analysis of NGF mRNA expression following anaphyla- toxin stimulation was also studied ... mRNA expression was increased by 3.2 fold after 2 h of stimulation by MAP-C 3a and by 2.9 fold after 4 h for MAP-C 5a. Upregulation of NGF mRNA expression induced by MAP-C 3a or MAP-C 5a was also ... 160:3543-3554. 30. Sayah S, Ischenko AM, Zhakhov A, Bonnard AS, Fontaine M: Expres- sion of cytokines by human astrocytomas following stimula- tion by C 3a and C 5a anaphylatoxins: specific increase in interleukin-6...

Ngày tải lên: 19/06/2014, 22:20

... th e wafer into an HF/AgNO 3 aqueous solution of a given concen- tration for an a ppropriate time. The reaction that takes place is a galvanic displacement reaction. A galvanic cell is established ... formation rate of SiNWs on non-patterned and on lithographically defined areas. Arrhenius plots of the etch rate of Si by MACE as a function of temperature in the case of non-patterned areas (dark ... images of SiNWs formed on the blank Si wafer and on lithographically defined areas. Cross-sectional SEM images of SiNWs fabricated on non-patterned Si areas (a) and on 100 ì 100-m 2 lithographically...

Ngày tải lên: 20/06/2014, 22:20

... Y, Saitoh Y, Sakai K, Satoh H, Odahara S, Nakano H, Nakagaki J, Shiota Y: Atomic-beam alignment of inorganic materials for liquid- crystal displays. Nature (London) 2001, 411:56-59. [3] Janning ... The polarized UV irradiation technique has many merits such as a clear image without the mura at a black level and an easy creation of multi-domain in a pixel. However, it also has a problem ... image taken on a sample before ion beam treatment. On the other hand, Figure 3b,c shows droplet images taken parallel and perpendicular to the ion beam-irradiated direction for the ion beam-treated...

Ngày tải lên: 20/06/2014, 23:20

... colloidal ink was spin-coated onto a full wafer-scale silicon substrate to form a multilayered nanosilver film (Fig. 2a) and transformed to an isolated random array of nanosilver islands by thermal annealing ... the antireflection effect was substantially large in general, and depends on the nanopillar height. The reflection nearly disappeared in the UV wavelength range and was only 17% of that of a bare ... measured in the Fig. 6 Quantitative analysis of nanosilver islands in terms of the concentration and spin speed: a average size, b coverage rate Table 1 Quantitative analysis of nanosilver islands...

Ngày tải lên: 21/06/2014, 17:20

... EXPRESS Fabrication of Densely Packed AlN Nanowires by a Chemical Conversion of Al 2 O 3 Nanowires Based on Porous Anodic Alumina Film Zhi-Hao Yuan ặ Shao-Qing Sun ặ Yue-Qin Duan ặ Da-Jian Wang Received: ... of the AlN nanowires than that of the Al 2 O 3 ones. Summary and Conclusions We have developed a convenient method for the prepara- tion of densely packed AlN nanowires based on a new strategy. ... follows: Al 2 O 3 nanowireịỵ4Al ẳ 3Al 2 Onanowireị: And then the following reaction can take place: Al 2 Onanowireịỵ2NH 3 ẳ 2AlNnanowireịỵH 2 O ỵ 2H 2 : As a result, the Al 2 O 3 nanowires are chemically converted...

Ngày tải lên: 21/06/2014, 20:20

Ngày tải lên: 05/08/2014, 09:46

Ngày tải lên: 07/08/2014, 18:21

Ngày tải lên: 08/08/2014, 01:21