preparing protein probe for overlay analysis

... bovine DNA probes for the screening of chromosomal abnormalities In this work, we isolated and cloned seven repetitive probes, one of which hybridised to a limited number of chromosomes This probe ... 11 bands were cloned DNA sequence analysis revealed 90 - 95% identity with Bos taurus repetitive sequences (satellite or SINE) for five of the seven probes One probe (AF124263) revealed no homology ... hybridised with the W5 probe b A 59XXt4.10 cell line hybridised with the E1A probe c A 59XXt4.10 cell line hybridised with the H1A probe d A normal 60XX cell line hybridised with the H1A probe Apart from...

Ngày tải lên: 09/08/2014, 18:21

Ngày tải lên: 09/12/2013, 18:15

... Pick a point on the drawing for placement of the note – just above 45º x 02 Note that the dimension automatically switch to their symbolic form Note the parametric symbol for the chamfer d22 Be sure ... view style Select Hidden for Display style OK 11 Pick the right and isometric views, make sure it is boxed in red Right-click, and select Properties Select No Hidden for Display style OK 12 Add ... Done/Return Move the note if necessary 28 Redefine the Shaft for Correct Dimensioning It is a common to dimension the axial locating dimension for the snap ring groove from the end of shaft rather than...

Ngày tải lên: 12/12/2013, 12:15

... infrared gas analysis Measurements were performed during the first h 508 of the light phase, representing the time period of photosynthetic activity until plants were harvested for analysis of ... electrode For starch extraction, pellets for ethanol extraction were solubilized by heating them to 95 °C in 0.5 m NaOH for 30 After acidification with m CH3COOH, the suspension was digested for h ... search method for bound constrained gloabal optimization J Glob Optim 39, 197–219 34 Schmidt H & Jirstrand M (2006) Systems Biology Toolbox for MATLAB: a computational platform for research in...

Ngày tải lên: 14/02/2014, 22:20

... compensation for holding these instruments in the form of interest rate remuneration and monetary services The monetary services compensate the depositors for the interest they forego by holding ... banks has considerable implications for the macroeconomy The examination of money and credit developments for the purposes of monetary analysis provides information on the functioning of banks’ ... (see Box entitled “Multiplier analysis of the effect of monetary policy on money supply”) The supply of money – bank behaviour and the implications for monetary analysis See Brunner, K and Meltzer,...

Ngày tải lên: 16/02/2014, 11:20

... et al., 1996) is a probabilistic model for information retrieval and is one of the most popular and effective algorithms used in information retrieval For ease of reference, we incorporate the ... comparison of event models for naive bayes text classification 1394 G Mishne 2005 Experiments with mood classification in blog posts In 1st Workshop on Stylistic Analysis Of Text For Information Access Fabrizio ... other class and subtracting the two values Therefore, the weight of term 1388 Table 2: SMART notation for inverse document frequency variants For ease of reference we also include the BM25 idf...

Ngày tải lên: 20/02/2014, 04:20

... sentiment analysis The success of delta idf weighting in previous work suggests that incorporating sentiment information into VSM values via supervised methods is helpful for sentiment analysis ... latent semantic analysis Journal of the American Society for Information Science, 41:391–407, September R E Fan, K W Chang, C J Hsieh, X R Wang, and C J Lin 2008 LIBLINEAR: A library for large linear ... graphical models for statistical language modelling In Proceedings of the ICML, pages 641–648 G Paltoglou and M Thelwall 2010 A study of information retrieval weighting schemes for sentiment analysis...

Ngày tải lên: 20/02/2014, 04:20

... Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material Information, descriptions ... temperature of -5oC, and a narrow ID torch to maintain plasma stability Performance The ICP-MS sample introduction setup for the analysis of volatile organic solvents such as isopropyl alcohol (IPA) ... addition, which is optimized for each different sample type With this setup, the analysis of organics is stable and reproducible Figure shows a 4-hour stability plot for a series of trace elements...

Ngày tải lên: 05/03/2014, 21:20

... small businesses  Uniform SBIR Portal for Information and Solicitations For the Small Business Innovation Research program, there is no one form or database for applying for the program and submitting ... Application for 7(a) Loans Analyze Automated Credit Decision Model for 7(a) Loan Program Analyze Uniform SBIR Portal For Information and Solicitations Analyze Single Uniform Certification for SBA ... expect that information gathered will also inform the regulatory process review generally Information on the SBJA Tour can be found at www.sba.gov/jobsacttour IV Current Agency Efforts Already...

Ngày tải lên: 07/03/2014, 02:20

... Several drops of chloroform were further added, and the resultant solution was kept at C for days The solution was then evaporated under reduced pressure, and the precipitate formed was collected ... 531.3 Elemental analysis calculated (%) for C112H164O72N6ặ2CuCl2: C, 44.59; H, 5.44; N, 2.79 Found: C, 44.85; H, 5.72; N, 3.04 Redox iodometric titration of copper (II) was also performed to establish ... molặL)1; [2] = 4.00 ã 10)5 molặL)1; pH 6.00 $ 10 and remained constant for at least h Hence, the reaction was left to proceed for 10 min, and the uorescence was then measured at room temperature...

Ngày tải lên: 07/03/2014, 05:20

... be seen to perform moderately complementary, where EEG scores best for arousal, peripheral for valence and MCA for liking Of the different rating scales, valence classification performed best, ... training sets First, the features for the given modality are extracted for each trial (video) Then, for each participant, the F1 measure was used to evaluate the performance of emotion classification ... concern: physiological signals analysis for emotion assessment and brain-computer interaction, multimodal interfaces for blind users, data hiding, multimedia information retrieval systems Anton...

Ngày tải lên: 07/03/2014, 14:20

... and conformational analysis of the 2P-containing duplexes, the substitution of 2P for C could be a good probe for DNAprotein interactions in the major groove of DNA Substitution of 2P for C appears ... nucleotide synthesis, S Klimas auskas for M.HhaI, A S Bhagwat for plasmid pT71 used for construction of a hybrid plasmid carrying the gene for M.EcoRII and O V Kirsanova for help on purication of M.EcoRII ... AdoHcy for 15 at room temperature and 10 at C The reaction mixtures ể FEBS 2004 2-Pyrimidinone as a probe for DNA methyltransferase (Eur J Biochem 271) 2393 were analysed by 8% native PAGE for h...

Ngày tải lên: 07/03/2014, 15:20

... search for in the error typology, and then search backwards or forwards for error annotations of that type It is possible both to search for specific errors deep in the typology, and to search for ... LAST; a flexible tool for annotation of bilingual segments, specifically intended for error analysis of MT B LAST facilitates the error analysis task, which we believe is vital for MT researchers, ... HTER annotations for more information We created B LAST with the goal that it should be flexible, and allow maximum freedom for the user, 57 System Overview B LAST is a tool for human annotations...

Ngày tải lên: 07/03/2014, 22:20

Ngày tải lên: 08/03/2014, 00:20

... to transform syntactic as well as morphological variations of names into a normalized name form Basic transformations can be achieved via pattern matching (regular expressions) while for more ... transformations a linguistic parser, yielding a syntactic analysis, would be needed For example, the names glyceraldehyde-3-phosphate and 3-phospho-Glyceraldehyde could both be normalized to the form ... than some other format, because SMILES themselves use the concept of a graph for representing the molecular structures (Weininger, 1988) For example, the graph solution determined for pentan-2,3-diol...

Ngày tải lên: 08/03/2014, 01:20

... arises for immature animals on poor quality forage, to what extent can growth be increased by providing nutrients for the rumen and extra protein for absorption? The provision of more protein for ... demand for food for humans and feed for livestock Grain for livestock, will have to compete with increasing demand for grain to produce industrial alcohol The latter arises from the demand for industrial ... obvious associated errors Response to escape protein of young ruminants given low digestibility forage Credit for the discovery of the need for escape protein in the diets of producing ruminants...

Ngày tải lên: 08/03/2014, 23:20

... elastic modules for 3-phase composite plate The elastic modules for 3-phase composite are calculated step by step by 2-phase model as in [5,6,8] We also carried out the experiments for 3-phase composite’s ... plate’s center is given in Table Table Comparison between analysis and experiment for bending deflection 3-phase composite Concentrate force 1kN 3kN 6kN 10kN Ultimate deflection 7,6 8,1 6,57 6,0 ... + 60% AKAVINA polyester (sample type B) Experiment* Analysis Error % Experiment* Analysis Error % Note: *is the average result after tests for one sample type From the result in Table 1, we can...

Ngày tải lên: 14/03/2014, 13:20

... as unbound protein (U), washed samples (W), eluted GST–MP (E) and purified GST (G) Lane M, protein molecular mass markers Arrows indicate the position of purified proteins (B) Protein protein interaction ... concentration (Fig 5B,C) The formation of salt bridges and hydrogen bonds crucial for protein protein interactions is dependent on pH Variation in pH may also lead to conformational changes that ... ELISA for monitoring protein protein interactions rMP was dialysed against assay buffer (20 mm Tris ⁄ HCl, pH 8) The stability of the protein in the assay buffer was checked by storing the protein...

Ngày tải lên: 15/03/2014, 00:20

... some effort) Structural information can be subsequently downloaded by the users as a text-formatted file For a structure with the accession code 9xyz, the corresponding file would be 9xyz.pdb (For ... collected for phasing For refinement, it is most important to collect a complete data set to the resolution limit of Protein crystallography for non-crystallographers diffraction, whereas for phasing ... Ramakrishnan C & Ramachandran GN (1965) Stereochemical criteria for polypeptide and protein chain conformations II Allowed conformation for a pair of peptide units Biophys J 5, 909–933 Brunger AT,...

Ngày tải lên: 16/03/2014, 06:20