prepared as a bride adorned for her husband

Ngày tải lên: 05/09/2013, 14:58

Ngày tải lên: 05/09/2013, 15:28

... followed by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned into ... [43–49]. Acknowledgements The authors thank Drs Masato Yasui, Sadakazu Aiso and Masaaki Matsuoka for support; Dr Andrew P. McMahon for providing the full-length mou- se Shh cDNA; Dr Neil Cashman for providing ... COS7 cells affecting the 5E1 epitope. Gup1 acts as a negative regulator for N-terminal palmitoylation of Shh Mammalian Gup1 has been described in the gene data- base cited above as a homolog of...

Ngày tải lên: 18/02/2014, 16:20

... physiological rather than an artifact of lysis. These results also support, using a visual rather than molecular biochemical approach, the proposal that PMA triggers increased interaction between PLD1 and ... plasma membrane, which is also required for NADPH activation [9,10]. Once NADPH oxidase is activated, it generates H 2 O 2 , which can func- tion to kill intracellular bacteria and play pro-apopto- tic ... lysate was spun down at 50 000 g for 15 min. The supernatant was mixed with pre-equilibrated anti-HA affinity matrix and rocked at 4 °C for 2 h. The lysate ⁄ matrix was pelleted and washed three times...

Ngày tải lên: 20/02/2014, 01:20

... growth NITZS54 Nitzschia Bacillariop hyceae 8 1003 ASU3004 Amphora Bacillariop hyceae 9 593 FRAGI2 Fragilaria Bacillariop hyceae 6 304 AMPHO27 Amphora Bacillariop hyceae 38 235 ... others. Thus, fewer site- specific studies are required for microalgae than, for example, tree farming. Also, microalgae grow much faster than higher plants and require much less land areas. ... step. After stirring the sample using magnetic stirrer bar for 5 h and ultrasonicated for 30 min, the samples were centrifuged at 3000 rpm for 10 min. The solid phase was separated carefully...

Ngày tải lên: 21/02/2014, 10:20

... NITZS54 Nitzschia Bacillariop hyceae 8 1003 ASU3004 Amphora Bacillariop hyceae 9 593 FRAGI2 Fragilaria Bacillariop hyceae 6 304 AMPHO27 Amphora Bacillariop hyceae 38 235 MAKARA, TEKNOLOGI, ... increase from about 5 to 6.4 and constant around this value which was the same pH range as that using lower CO 2 flow rate. As the growth recovered at the same time during the gradual increase ... utilizing an algal biomass before degradation. There are several ways to make biodiesel, and the most common way is transesterification as the biodiesel from transesterification can be used...

Ngày tải lên: 21/02/2014, 10:20

... because of an imbalance in the activity of tau kinases and phospha- tases [3]. One particular tau kinase pertinent to metal dyshomeostasis in AD is glycogen synthase kinase-3 (GSK3). GSK3 has recently ... ablated [41]. Amyloid-b After the 4.5 kDa Ab peptide was identified as a major component of the amyloid plaques in AD brain [42,43], global AD research focused on this peptide as a causative agent ... Furthermore, an increase in extracellular metals can catalyse Ab oligomerization and aggregation, and the amyloid plaques that subsequently form may then exacerbate intracellular metal deficiency by...

Ngày tải lên: 07/03/2014, 10:20

... measured by the Bradford method [51] using a Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA) with bovine c-globulin as a standard. Thyroglobulin (669 kDa), catalase (232 kDa), BSA (67 kDa) and ... weight) )1 at stage 1, which was less than half of that in ovary at stage 1, and remained at a similar value to stage 4. Testes at stage 2 were not analyzed because of a lack of samples. We calculated the ... sea urchin ) implications of its role as a zinc transporter for gametogenesis Tatsuya Unuma 1 , Kazuo Ikeda 2 , Keisuke Yamano 3 , Akihiko Moriyama 4 and Hiromi Ohta 5 1 Japan Sea National Fisheries...

Ngày tải lên: 16/03/2014, 05:20

... the performance of rosetta using multiple sequence align- ment information and global measures of hydrophobic core formation. Proteins 43, 1–11. 11 Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, ... NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr, Sect D 54, 905–921. 30 Laskowski RA, Rullmann JAC, MacArthur MW, Kaptein R & Thornton JM (1996) AQUA and ... Free induction decay values with on- and off-resonance protein saturation were recorded in alternative fashion. Subtraction of the 1D STD spectra was achieved via phase cycling. Pro- tein resonance was suppressed...

Ngày tải lên: 16/03/2014, 23:20

... the later examples. Variables constitute an additional device available in DATR but are assumed to have the status of abbreviations. Despite their syntactic similarities, DATR and PATR differ ... THE DATR LEXICON It has been shown above that DATR theories can serve as a lexicon for a PAT R system where the lexemes are represented as DATR nodes and the returned values of queries are ... evaluation. As a declarative language, DATR is independent of the procedural evaluation strate- gies embodied in particular DATR-implementa- tions. Nevertheless, DATR theories like (4) may...

Ngày tải lên: 18/03/2014, 02:20

... carnitine palmitoyltransferase I, mitochondrial carnitine acylcar- nitine translocase and carnitine palmitoyltransferase II [5–7]. In case of the straight-chain and 2-methyl-branched chain FAs, ... oxidation Beta-oxidation is the preferred way of oxidizing FAs. In principle, each FA can be b-oxidized, including straight- and branched-chain FAs, as well as mono- and polyunsaturated FAs. There ... arachidonic acid x-hydroxylase in human liver and kidney with a higher substrate specificity for arachidonic acid than the already established arachi- donic acid x-hydroxylase CYP 4A1 1 [44,45]. The formation...

Ngày tải lên: 22/03/2014, 16:21

... 1105–1111. 10 Tamada Y, Nakao K, Nagayama Y, Nakata K, Ichikawa T, Kawamata Y, Ishikawa H, Hamasaki K, Eguchi K & Ishii N (2002) p48 Overexpression enhances interferon-mediated expression and activity of ... maximal pathway activation and an increase in signal amplitude [3,4]. Furthermore, alterations in the activity of a kinase or phosphatase may affect the speed of signalling pathway activation. However, ... theoretical analysis and experimental data generation reveals IRF9 as a crucial factor for accelerating interferon a- induced early antiviral signalling Tim Maiwald 1, *, Annette Schneider 2, *, Hauke...

Ngày tải lên: 23/03/2014, 03:20

... Absence Cyanidium caldarium Chloroplast DNA Absence Absence Presence 45% (2e)30) Absence Bacillariophyceae (diatoms) Thalassiosira pseudonana Nuclear DNA ? ? Absence Presence 35% (4e)10) Chloroplast DNA Absence ... Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Nishida K, Yoshida Y et al. (2004) Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature ... Absence Chloroplast DNA Absence Absence Absence Absence Higher plant Oryza sativa Nuclear DNA Presence 82% (1e)88) Presence 69% (2e)44) Absence Absence Chloroplast DNA Absence Absence Absence Absence I. Enami...

Ngày tải lên: 23/03/2014, 15:21

... incomplete ms an account of the structure of a natural language, e.g. a TAG grammar wW contain ~th an active and a passive form of the same verbal sutx:ategurization pattern, without an theory-mediated ... orientation makes systemic grammars more immediately useful than, for example, tramffotmationai generatb,+ grammars or even procedurally oriented AI fogmali-qa~s |of language such as ATN's. ... Grammars, or "TAG's', (Josh/, Levy & Takahash/ 1975; Josh/ 1983; Kroch & Josh/ 1965) we~ developed as an al~ma~ive to the aandard tyntac~ formalisms that are ,,_~'~...

Ngày tải lên: 24/03/2014, 02:20

... Kurukh KUS Kusaiean LAO Lao LAV Latvian LIN Lingala LIT Lithuanian LUA Luba-Lulua LUO Luo MAC Macedonian MAD Madurese MLG Malagasy MAY Malay MAL Malayalam MLT Maltese MAR Marathi MAH Marshallese MEN ... Arabic ARM Armenian ASM Assamese AZE Azerbaijani BAM Bambara BAK Bashkir BAQ Basque BEL Belarusian BEM Bemba BEN Bengali BER Berber BIK Bikol BOS Bosnian BUL Bulgarian BUR Burmese CAT Catalan CEB Cebuano NYA ... Ethiopia, Gabon, Gambia, Ghana, Guinea, Guinea Bissau, Kenya, Lesotho, Liberia, Madagascar, Malawi, Mali, Mauritania, Mauritius, Mozambique, Namibia, Niger, Nigeria, Reunion, Rwanda, Sao Tome and...

Ngày tải lên: 25/03/2014, 10:41

... putative signal peptide was constructed as follows. The DNA fragment was amplified from GmPDIM cDNA by PCR using the primers 5¢-GACGACGACAAGATGC ACGCACTCTATGGAGC-3¢ and 5¢-GAGGAGAAGC CCGGTTCATAGCTCATCCTTGCTTGAAG-3¢. ... soybean cotyledons during matu- ration. (A) GmPDIM mRNA was quantified by real time RT-PCR. Each value was standardized by dividing the value by that for actin mRNA. Values are calculated as a percentage ... Mini kit. Quantifica- tion of mRNA was performed by real-time RT-PCR with a Thermal Cycler DiceÔ Real Time System (TaKaRa Bio Inc.). The forward primer 5¢-CGGAACCAAAACATGC TAACATTTTC[FAM]G-3¢ (Invitrogen,...

Ngày tải lên: 30/03/2014, 04:20

... IL2RA gene are associated with age at diagnosis in late-onset Finnish type 1 diabetes subjects. Immunogenetics 2010, 62:101-107. 36. Kawasaki E, Awata T, Ikegami H, Kobayashi T, Maruyama T, Nakanishi ... cellular and molecular mechanisms of its action on Treg cells. We then move on to describe how this data can be translated to applica- tions for human autoimmune diseases by using IL-2 as a therapeutic ... 2003, 171:3348-3352. 13. Kumanogoh A, Wang X, Lee I, Watanabe C, Kamanaka M, Shi W, Yoshida K, Sato T, Habu S, Itoh M, et al: Increased T cell autoreactivity in the absence of CD40-CD40 ligand interactions: a role...

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 18/06/2014, 19:20