preparation of the substrate

... assessment. Institute of Energy RURAL RENEWABLE ENERGY WEEK IN HANOI EXPERIENCE OF THE INSTITUTE OF ENERGY IN THE PREPARATION OF THE RENEWABLE MASTER PLAN Presented by: Nguyen Duc Cuong Institute of Energy Hanoi, ... MW Geothermal energy and other types (tide, ocean waves ) ¾ Potential of geothermal energy resources: 200- 340 MW. ¾ The renewable energy type such as tide and sea waves are in the stage of potential ... energy, and geothermal energy. 9 So far, even the high potential of RE resources, their contribution in electricity production in Vietnam is still negligible. 9 The Government of VN recognized...

Ngày tải lên: 24/01/2014, 03:20

... sub- contract from The Of ce of Biomass Program, within the DOE Of ce of Energy Efficiency and Renewable Energy. Tom Desmet holds a fellowship of the Insti- tute for the Promotion of Innovation through ... presumably, contain the sites of O-glycosylation, but we have not identified the specific site(s) or the form of the O-glycans. The CD spectrum of Cel74A looks a lot like that of an unordered protein ... comparing the activity of Cel74A on 0.1 mm XXXG, determined as described above, in the presence and absence of 2 mm inhibitor. The inhibitors were not hydro- lyzed in the time course of these experiments...

Ngày tải lên: 19/02/2014, 05:20

... underscoring the importance of the interactions between the Tyr OH and the side chains of Asn174 and Asp148 in TEV protease (Fig. 4A). The importance of these interactions is fur- ther supported by the ... effects of the mutations. Hence, the results are consistent with the predicted role for these residues (based on crystal structure) in the interaction with substrate. The loss of activity of the ... modeling, the Arg side chain of the substrate may partially compensate for the loss of the Lys side Table 1. Comparison of the specificity of TEV and TVMV proteases. The relative specificity constants...

Ngày tải lên: 19/02/2014, 16:20

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Ngày tải lên: 05/09/2013, 16:11

... / VNU Journal of Science, Mathematics - Physics 25 (2009) 1-7 6 the magnetic squareness M r /M s reduced with J because of the presence of the Fe 3 Pt. Of cause, the kink in the magnetic ... of the samples. J is the current density; t on is the deposition time of a pulse; Fe, Pt are the atomic percent of Fe and Pt in the samples, respectively; H c is the coercivity; M s is the ... with diameter of 1.3 cm acted as both the cathode and ultrasound emitter (Sonics VCX 750). The electroactive part of the sonoelectrode was the planar circular surface at the bottom of the Ti horn....

Ngày tải lên: 13/02/2014, 03:20

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... by Southern analysis. The rluD::cat strain had a full deletion of both rluD and yfiH, together with their annotated pro- moter sequences. The inserted cat cassette had a distance of 90 nt from the ... induces reorganization of the RNA [21]. An ability of the RNA substrate to adopt the alternative fold with a reverse Hoogsteen base pair is used by RluA to recognize its substrate [21]. It is...

Ngày tải lên: 18/02/2014, 16:20

... rigid aromatic side chain at the P2 position of the substrate in the case of papain [26,41]. Ervatamin-B shows Pro specificity at the P2 position of the substrate. The S2 subsite of ervatamin-B is lined with ... proton from the catalytic Cys pres- ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The first stage of catalysis is mediated by the highly active thiolate ion of the Cys. Biochemi- cal ... erva- tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent (Fig. 2B). On the other hand, the P3...

Ngày tải lên: 18/02/2014, 16:20

... with the substrate aglycone but do not participate in the binding of the substrate glycone. Therefore, an implication of these results is that the interactions with aglycone do not affect the ... Thus, the effect of the alteration of the glycone structure in the stabil- ity of the ES à complex (DDG à ) for the wild-type and mutant Sfbgly was determined using the k cat ⁄ K m for the hydrolysis ... degree of polymerization equal to 1. Linear free energy relationships The effect of the alteration of the substrate structure on the stability of the complex ES à was evaluated using the follow- ing...

Ngày tải lên: 18/02/2014, 17:20

... phosphorylation of the wild-type. Relative phosphorylation expressed as a relation of the percentage of dThd phosphorylation of the corresponding mutant is given in paren- theses. The substrate concentration ... treatment of cancer and viral diseases because they catalyse the addition of the first phosphate group to the nucleoside analogue. This primes the nucleoside for further phosphorylation to the corresponding ... true, a profound effect on the catalytic rate should be evident from these mutations. Binding of the substrates to the enzyme should not be altered to the same extent, as reflected by the lower...

Ngày tải lên: 19/02/2014, 02:20

... wild-type enzyme, whereas the K22E mutant protein failed to form the covalent adduct. On the other hand, the K22V mutant protein requires the presence of UDPNAG for the formation of the adduct indicating ... wall biosynthesis are attractive targets for the development of new drugs [1]. The biosynthesis of the cell wall component peptidoglycan (or murein) commences with the transfer of the intact ... binding. The loop region of the upper domain moves towards the active site of the enzyme, thus shielding the substrates (and ligands) from bulk solvent. A detailed thermodynamic study of this...

Ngày tải lên: 19/02/2014, 13:20

... 74% of the cases; residue 186 an Asn in 87% of the cases; and residue 334 a His in 80% of the cases [3]. In addition, residue 54 is a Pro in 89% of the cases. The importance of the identity of the residues ... [4–6]. The subsequent RCL insertion into b sheet A therefore results in an < 7-nm translocation of the proteinase from the position of its initial encounter with the RCL to the other pole of the ... we reach the conclusion that the substitutions in the shutter region affect the rate of latency transition by affecting the rate of passage of the RCL through the gate region. Based on the amino- acid...

Ngày tải lên: 22/02/2014, 07:20

... different flavonoids. The addition of a flavone (apigenin), of a flavanonol (taxifolin) and of a flavan-3-ol (epicatechin) (Fig. 8) did not alter the EPR spectra of the enzyme indicating the absence of binding to the ... definition of some of the catalytic steps. Binding of the various flavonols to the active site metal centre is found not to require the presence of dioxygen and occurs without formal reduction of the ... reaction is rather unusual in that it involves the cleavage of two carbon–carbon bonds and the concomitant production of carbon monoxide. The stoichiometry of the process is such that 2 mol of substrate...

Ngày tải lên: 22/02/2014, 07:20

... For comparison, the amount of GroEL was determined in the same extracts using anti-GroEL Ig. It has been previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock ... respectively. In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts of the purified UP12 were separated ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not have any detectable effects on the sensitivity to mitomycin C, unlike in the case of...

Ngày tải lên: 22/02/2014, 07:20

... translocation of UDP- glucose pyrophosphorylase 1 to the plasma membrane, where it increases cell wall glucan synthesis at the expense of glycogen storage. In the absence of PSKs, glycogen rather than ... (data not shown). On the basis of the known functions of the PASKIN-related FixL oxygen sensor in bacteria and the PASKIN orthologues in yeast, and considering the lack of any obvious phenotype ... microarray of 1176 peptides in the presence of [c- 33 P]ATP. The magnified inset shows an example of the results obtained after detection by phosphorimaging. (B) Peptide sequences of the 30 most...

Ngày tải lên: 06/03/2014, 00:21