preparation of poly a mrna from bladder and mgh samples

Mycobacterium bovis, BCG modulation of gene expression in bladder cancer

Ngày tải lên : 11/09/2015, 10:06

... SuperArray’s pathway specific OligoArrays……… 46 2.2.4.5.1 Preparation of poly A+ mRNA from bladder and MGH samples ……………… 46 2.2.4.5.2 Linear amplification of poly A+ mRNA and preparation of Biotin-16-UTP ... Advanced bladder cancer Stages T2 and T3 of cancer development are still localised within the bladder while the T4 stage denotes the advanced stage of bladder cancer, where cancer cells metastasize and ... bladder sparing strategies are used to manage patients with advanced metastatic disease or other medical complications, such as cardiovascular disease 1.2 BCG immunotherapy of superficial bladder...

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PURIFICATION OF ORGANIC CHEMICALS pot

Ngày tải lên : 05/03/2014, 21:20

... If it is not satisfactory then add Ac20 and a drop of conc H2SO4 and heat at 50° for l0min Then add anhydrous NaOAc ( c a 3g/ lOOg of liquid) and fractionate Note that it forms an azeoptrope with ... include H20, acetamide, NH40Ac and NH3 Anhydrous CaS04 and CaC12 are inefficient drying agents Preliminary treatment of acetonitrile with cold, satd aq KOH is undesirable because of base-catalysed ... 200g of freshly activated alumina (which had been reactivated by heating at 250° for 4h) The decanted solvent was again shaken with activated alumina, followed by five batches of 100-15Og of anhydrous...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... Acetonitrile and acetone of chromatographic grade were purchased from Merck (Darmstadt, Germany) The essential oil of Curcuma wenyujin was purchased from Zhejiang Ruian Pharmaceutical (Ruian, China) The ... yielding nearly 50 mL of the stationary phase eluted after hydrodynamic equilibrium was attained, and the maintenance of the stationary phase was also improved The purity of the target components from ... (peak area of target compound)/(total peak area of all peaks) Table The retention times (RT) and MS data of six isolated components Peak Compound RT (min) MS dataa) Curdione Curcumol Germacrone...

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PURIFICATION OF LABORATORY CHEMICALS pot

Ngày tải lên : 14/03/2014, 20:20

... Preparation and standardisation of alumina Preparation of other adsorbents Silica Diatomaceous earth Charcoal Cellulose Supelco products Flash Chromatography (FC and HPFC) Paired- ion Chromatography ... PRECAUTIONS ASSOCIATED WITH THE PURIFICATION OF LABORATORY CHEMICALS SOME HAZARDS OF CHEMICAL MANIPULATION IN PURIFICATION AND RECOVERY OF RESIDUES Perchlorates and perchloric acid Peroxides Heavy ... chromatography Liquid chromatography 17 17 (GC or gas-liquid Adsorption chromatography Thin layer chromatography (TLC) Paper chromatography Column chromatography Graded adsorbents and solvents Preparation...

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purification of carbon nanotubes

Ngày tải lên : 20/03/2014, 13:06

... homogeneity of CNT samples can be evaluated by standard deviations of the oxidation temperature and metal content obtained in several separate TGA runs [29] The real difﬁculty is qualitative or quantitative ... 2006;128(30):9902–8 [89] Coleman JN, Dalton AB, Curran S, Rubio A, Davey AP, Drury A, et al Phase separation of carbon nanotubes and turbostratic graphite using a functional organic polymer Adv Mater 2000;12(3):213–6 ... changes of single-wall carbon nanotubes and single-wall carbon nanohorns caused by heat treatment Carbon 2003;41(6):1273–80 Koshio A, Yudasaka M, Iijima S Disappearance of inner tubes and generation...

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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

Ngày tải lên : 29/03/2014, 09:20

... substrates in Arabidopsis thaliana Plant J 25, 487–498 22 Sakuraba H, Kawakami R, Takahashi H & Ohshima T (2004) Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis J Bacteriol ... microorganisms Methods Enzymol 113, 83–89 17 Sung MH, Tanizawa K, Tanaka H, Kuramitsu S, Kagamiyama H & Soda K (1990) Puriﬁcation and characterization of thermostable aspartate aminotransferase from ... VosWM & van der Oost J (2002) Molecular analysis of the role of two aromatic aminotransferases and a broad-speciﬁcity aspartate aminotransferase in the aromatic amino acid metabolism of Pyrococcus...

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armarego - purification of laboratory chemicals 5e

Ngày tải lên : 04/06/2014, 15:52

... Silylation of Glassware and Plasticware Safety Precautions Associated with the Purification of Laboratory Chemicals Some Hazards of Chemical Manipulation in Purification and Recovery of ... used as an antioxidant) Although it was only an artefact of the separation procedure it has been isolated as an apparent component of vitamin K preparations, extracts of plant lipids, algae, livers, ... OV-17 and Carbowax-20M The use of capillary columns in gas Chromatography for analysis is now routinely carried out An extensive range of packed and capillary columns is available from chromatographic...

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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot

Ngày tải lên : 18/06/2014, 18:20

... MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft ... centrifugation in a table top Eppendorf centrifuge DNA extraction and quantitative real-time TaqMan PCR Extraction of DNA and determination of viral DNA copies for HHV- 6A was performed as previously ... Fig 2A and 2B, a considerable amount of protein containing material from both HHV- 6A and mock preparations was detected in the top fractions of the iodixanol gradient while the bottom fractions...

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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx

Ngày tải lên : 20/06/2014, 01:20

... MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft ... centrifugation in a table top Eppendorf centrifuge DNA extraction and quantitative real-time TaqMan PCR Extraction of DNA and determination of viral DNA copies for HHV- 6A was performed as previously ... Fig 2A and 2B, a considerable amount of protein containing material from both HHV- 6A and mock preparations was detected in the top fractions of the iodixanol gradient while the bottom fractions...

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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc

Ngày tải lên : 20/06/2014, 01:20

... NP bac NP 3' NP bac Oligonucleotide Primer Sequence TTTCAGAATTCGGATCCACCAGTCTTTATAAAGGGGTTTAT GGTACCAAGCTTTCAGTCATAGCAATCTTCTACTAATATAAATATCTCT TTTCAGAATTCGGATCCGGCACATTCACATGGACACTG GGTACCAAGCTTTCAGCTATGTCTTCCCCTGCCTCTCCAT ... GGTACCAAGCTTTCAGCTATGTCTTCCCCTGCCTCTCCAT TTTCAGAATTCAGTGCCTCAAAGGAAATAAAATCCTTTTTGTGGACACAATCTTTGAGGAG GGAATTATCTGGTTAC GGTACCAAGCTTTCAGTTACAGAACGACTCTAGGTGTCGATGT Note REN sites are underlined, and stop ... Branco, Alex Matschiner, and Darryl B Sampey are co-founders and sole members of the Board of Directors of BioFactura, Inc., and have received salary and other compensation from the company, such as...

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Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes" doc

Ngày tải lên : 20/06/2014, 01:20

... T7 RNA polymerase leads to the synthesis of run-off transcripts with the sequence: AGUAGAAACAAGGGUGUUUUUUCCCGGGAAUUCGGAUCCACACCCUGCUUUUG CUand AGCAAAAGCAGGGUGUGUGGAUCCGAAUUCCCGGGUAAAAAACACCCUUGUUUCUACU, ... of arrow represents the relative rate of each process At 37°C, polymerase-vRNA complexes are stable and catalyse the synthesis of mRNA and low amounts of cRNA Polymerase-cRNA complexes show a ... was clear that as the incubation temperature increased from 31°C to 39°C the amount of replicative RNA products (c- and vRNA) decreased and a greater accumulation of mRNA was observed This change...

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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

Ngày tải lên : 21/06/2014, 02:20

... investigated and benzyloxylactone, achieved from a norcamphor derivative, emerged as an important precursor for the insect antifeedant azadirachtin (Gagnon et al 1994,; Gagnon et al 1995b) A series of ... reproducible and reliable methods for separation and purification are required for the accurate characterization of these enzymes The availability of efficient cofactor recycling strategies for NADH-regeneration ... NdeI at the N-terminus and XhoI at the C-terminus (NdeI_2,5-DKCMO_fw: 5’- GGAATTCATATGAAA TGCGGATTTTTCCATACCCC-3’; 2,5-DKCMO_XhoI_rv: 5’- CCGCTCGAGTCAGCCCATTCGAACCTT3’) After initial denaturation...

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purification of some heavy metals from paper making

Ngày tải lên : 20/07/2014, 12:47

... include Avicennia marina[10],Kandelia candel[11 ]and Aegiceras corniculatum[12] Shuangtai estuary wetland is the final barrier of Shuangtai into Bohai Sea Water quality of Liaohe river has deteriorated, ... obtained as follows: multi-element standard solutions containing 56 species of metals (national standard sample from National Standard Material Net), concentrated H2SO4, concentrated HNO3 and concentrated ... and its heavy metal content has exceeded national surface water standards.[13] Research on the reduction of copper(Cu),zinc(Zn),lead(Pb) and cadmium(Cd) in paper-making wastewater by Shuangtai...

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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps

Ngày tải lên : 11/08/2014, 00:23

... purification of His-tagged proteins and presents a major limitation for broad application of such materials In this regard, optimization and evaluation of commercially available matrices is mandatory, ... biomedicine Biomagn Res Technol 2003, 1:2 Sakamoto S, Kabe Y, Hatakeyama M, Yamaguchi Y, Handa H: Development and application of high-performance affinity beads: toward chemical biology and drug discovery ... 17 and 11 kDa was from Fermentas (St Leon-Rot, Germany) Reagents for Bradford protein assay were purchased from Bio-Rad Laboratories (Bio-Rad Laboratories, California, USA) All other chemicals...

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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx

Ngày tải lên : 11/08/2014, 21:21

... template to amplify the VP2 fragment by PCR The PPV VP2 gene was amplified with the primers PPV-VP2 FD (TATGGATCCGATGAGTCATCATCACCATCACCATAGTGAAAATGTGGAACAAC) and PPV-VP2 RV (GCGT CGACTATGAGTTAGAGTTTGTATTAG) ... obtained from Invitrogen (Carlsbad, California, USA) Prestained protein ladder was purchased from Fermentas International Inc (Burlington, Canada) Swine anti-PPV serum samples, with serum Page of ... Construction of recombinant plasmids and recombinant bacmid Genomic DNA was extracted from the cell-cultured strain 20-06 of PPV by the classical phenol-chloroform extraction method and was used as a template...

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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt

Ngày tải lên : 12/08/2014, 04:21

... immunodetection of fractions from asialofetuin affinity chromatography Analysis by Analysis by immunodetection of fractions from asialofetuin affinity chromatography Five mg of the glycoprotein asialofetuin ... triangles) Serum samples were diluted at 1/200, and levels of IgG anti-p24 were determined by ELISA Analysis was performed in duplicate and all sera samples were analyzed in triplicate and standard ... novel adjuvant-carrier On the other hand, the lack of an optimal adjuvant and carrier to enhance antiviral responses has been problematic in vaccine development against HIV [14] The use of adjuvant-carrier...

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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1

Ngày tải lên : 24/08/2014, 12:43

... 10) All of the aliquots contained a band at ~29 kDa with the exception of aliquot which appeared to be clear of any detectable contamination Based on the intensity of the bands, it appeared that ... regulation of cell cycling, and it is known to have an impact on cell adhesion and cell migration (Cardoso and Oliveira 2003; Pham et al 2003; Takeuchi and Baichwal 1995) Aberrations in its activation ... profile was measured with UV absorbance (Figure 4F) The protein content appeared to peak in aliquot and and then again in aliquot 5, and it was believed that both polyclonal antibody and SIMPL...

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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene

Ngày tải lên : 13/11/2014, 09:25

... I chitinase caused a great loss of activity (Andersen et al., 1997; Iseli-Gamboni et al., 1998) In addition, mutation of Tyr 123 of a Zea mays chitinase and a similar tyrosine of an Arabidopsis ... vacuole and have antifungal activity, while acidic chitinases are usually extracellular and show little antifungal activity It seems that extracellular chitinases are involved in generation of ... soybean and Arabidopsis contain one intron and two introns, respectively (Watanabe et al., 1999; Samac et al., 1990) Class III chitinase genes in Arabidopsis (Samac et al., 1990), Sesbania rostrata...

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purification of phytase from aspergillus fumigatus

Ngày tải lên : 06/10/2015, 12:59

... livestock areas, and also improving nutritional values of organic phosphate (phytate) (Lei and Porres, 2003) Phytases are found naturally in plants and microorganisms, particularly fungi Most of the ... purification of a high molecular phytase of the fraction 60  90% AS precipitation from A fumigatus 11 Phytase 87.7 kDa Figure SDS-PAGE analysis of phyase fractions from Phenyl sepharose and SP-Streamline ... 13(4):297-335 Greiner, Ralf, Lucineia Gomes da Silva and Sonia Couri 2009 Purification and characterisation of an extracellular phytase from Aspergillus niger 11T5 3A9 Brazilian Jf Microbiol 40:795-807...

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THE CONTRIBUTION OF CLAMS ON TIDAL FLAT PURIFICATION CAPACITY

Ngày tải lên : 05/09/2013, 09:08

... sediment weight; and t= time Analytical methods The DOC and NH4-N concentrations of pore water were measured with TOC analyzer (TOC500 0A, Shimadzu) and automatic water analyzer (AACS-III, Bran+Luebbee), ... between clams and microorganisms accelerated decomposition of organic matters in tidal flat area and increased nitrate removal capacity of tidal flat sediment Results of this study are useful ... sample also collected from 0-2 and 9-11 cm depth of sediment with (A1 ; A2 ) and without (B1; B2) clams, in order to evaluated NO3-N removal capacity and quinone analysis A replaceable ring of acrylic...

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