preparation of lyo bcg and live bcg

... Culture and BCG preparations………………………………………………… 37 2.2.1.1 Growth and Maintenance of bladder cancer cell lines…………………………… 37 2.2.1.2 Preparation of Lyo BCG and live BCG ………………………………………… 37 2.2.1.3 Preparation ... granulomas of the lungs [166] 26 Chapter 1.6 Live versus lyophilized BCG Lyophilized BCG (lyo BCG) is a preparation of live BCG that has been freeze dried for use in clinical practice After lyophilisation, ... differences and similarities of the responses engendered by live and lyophilized BCG and evaluate their cellular mechanisms of action The interaction between BCG and urothelial cells is dynamic and besides...

Ngày tải lên: 11/09/2015, 10:06

... 101111 AcOH and diluted with 50ml of H20, (B) 5g KI in lOOml of H20 and (C) 0.3g ninhydrin in lOml of AcOH and 90ml of H20 The spray is prepared by mixing (A) and (B) with 1.5ml of (C) and stored ... impurities are trimethylacetic acid and C9 and C13 acids Dissolve 15g of acid in CCl4 (3OOml) and shake with lOml of 15N aqueous NH3 and the ammonium salt separates and is collected Acid impurities ... refluxed for 2h with 15ml of MeOH and 2ml of 98% H2SO4 (cool when mixing this soh) Pour into 10 volumes of H20 and extract with the minimum volume of CHC13 to give clear separation of phases The extract...

Ngày tải lên: 05/03/2014, 21:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

Ngày tải lên: 07/03/2014, 21:20

... adsorbents and solvents Preparation and standardisation of alumina Preparation of other adsorbents Silica Diatomaceous earth Charcoal Cellulose Supelco products Flash Chromatography (FC and HPFC) ... Salts and organic esters of some inorganic acids Sol vents 5 5 6 6 METHODS OF PURIFICATION SOLVENT EXTRACTION OF REAGENTS AND SOLVENTS AND DISTRIBUTION DISTILLATION Techniques Distillation of liquid ... REMOVAL OF TRACES OF METALS FROM REAGENTS METAL IMPURITIES DISTILLATION SCAVENGER RESINS AND OTHER SUPPORTS USE OF ION EXCHANGE RESINS PRECIPITATION Removal of lead contaminants Removal of iron...

Ngày tải lên: 14/03/2014, 20:20

... oxidation include air [40–46], a mixture of Cl2, H2O, and HCl [47], a mixture of Ar, O2, and H2O [48–50], a mixture of O2, SF6 and C2H2F4 [51], H2S and O2 [52], and steam [53] High temperature oxidation ... Direct observation Precise content of carbon and metals Diameter, quality and conductivity of SWCNTs Conductivity feature and content of SWCNTs Accurate assessment of F-G on CNTs Elemental contents, ... and metal catalyst was developed Oxidative ions and acid ions dissolved in solution can evenly attack the network of raw samples, and therefore selection of oxidant type and precise control of...

Ngày tải lên: 20/03/2014, 13:06

... description of the characterization of bacterial GGT and AGT at an enzymatic and gene level Comparison of the amino acid sequences with known enzymes showed the phylogenetic position of 1880 each ... GPT and AGT activities were derived from the single enzymes AT1, AT2 and AT3, respectively (Fig 1) GGT activity was caused by AT1 and AT2, which exhibited 11 and 60 UÆ(mg purified protein))1 of ... purification, suggesting that the four kinds of activity in CFE were derived from only the three enzymes Purified AT1, AT2 and AT3 gave single bands of 44, 42 and 45 kDa on SDS ⁄ PAGE, respectively...

Ngày tải lên: 29/03/2014, 09:20

... inhalation of vapours and absorption of liquids and low melting solids through the skin In addition to the toxicity of solvents there is also the risk of their flammability and the possibility of eye ... Usually, centrifugation is preferred because of the greater ease and efficiency of separating crystals and mother liquor, and also because of the saving of time and effort, particularly when very small ... solubility of the substance in it, and the ease with which it can subsequently be removed Preparation and Standardisation of Alumina The activity of alumina depends inversely on its water content, and...

Ngày tải lên: 04/06/2014, 15:52

... inoculum, after hpi and 1, 3, and dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected ... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained...

Ngày tải lên: 18/06/2014, 18:20

... inoculum, after hpi and 1, 3, and dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected ... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained...

Ngày tải lên: 20/06/2014, 01:20

... interests Luis M Branco, Alex Matschiner, and Darryl B Sampey are co-founders and sole members of the Board of Directors of BioFactura, Inc., and have received salary and other compensation from the company, ... instructions (New England BioLabs) The addition of dithiothreitol (DTT) was necessary to prevent aggregation and precipitation of protein before and during Factor Xa cleavage of LASV GP1-MBP and GP2-MBP ... 0.2 g/L of uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43...

Ngày tải lên: 20/06/2014, 01:20

... interactions and viral RNA synthesis The size of arrow represents the relative rate of each process At 37°C, polymerase-vRNA complexes are stable and catalyse the synthesis of mRNA and low amounts of cRNA ... methanol-chloroform [74] and RNA was isolated by phenol:chloroform extraction and ethanol precipitation Polymerase binding assays The preparation of RNA transcripts labelled internally with [α-32P] GTP and ... 6, lanes 11 and 12) At 37°C levels of mRNA decreased and vRNA increased between and h p.i (Fig 6, compare lane to 3) At 41°C, mRNA and vRNA levels increased with time (compare lanes and 5) but...

Ngày tải lên: 20/06/2014, 01:20

... was introduced using the sites of restriction endonucleases NdeI and XhoI for cloning with the BCA-kit and a standard curve of BSA in the same buffer in a range of 2-0.005 mg/mL was used Samples ... Expression of recombinant 2,5-DKCMO in E coli BL21 was performed by cultivation at 37°C to an OD 600 of 0.5, than addition IPTG to a final concentration of 0.1 mM and shifting the culture to 20°C and ... affinity chromatography and characterization of the enzyme Material and methods Enzymes, chemicals and media Pfu+-polymerase was obtained from Roboclon (Berlin, Germany) and dNTPs from Roth (Karlsruhe,...

Ngày tải lên: 21/06/2014, 02:20

... marina[10],Kandelia candel[11 ]and Aegiceras corniculatum[12] Shuangtai estuary wetland is the final barrier of Shuangtai into Bohai Sea Water quality of Liaohe river has deteriorated, and its heavy ... absorption and distribution in wetland reed Researchers such as Weis[7], Windham[8], analyzed and compared distribution of toxic heavy metals and absorbency in reed, Aksoy[9] discussed the rules of migration ... pipes and then filtered and refrigerated Sample three well-growing strains in each pool and divide them into root, stem and leaf to be tested after processing and decomposition[14] Four kinds of...

Ngày tải lên: 20/07/2014, 12:47

... [5, 10, 20 and 40 μl of SiMAC-Nickel bead (100 mg/ml) corresponding to the final concentration of 1, 2, and mg/ml, respectively, and 5, 10, 20, 40 and 80 μl of SDS- PAGE and Western blotting ... yield, and percent of protein recovery and loss were determined for each Nickel-magnetic matrix, each Page 10 of 11 bead concentration and each recombinant protein The sum of the specific band densities ... Comparison of purification yield and protein recovery of three Nickel-magnetic matrices for His-ProT and His-Mre11 recombinant proteins Purification yield was defined as the sum of the percents of the...

Ngày tải lên: 11/08/2014, 00:23

... cultures of porcine origin The method is both labor intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and safety ... PPV-VP2 FD and PPV-VP2 FD The fragment was about 1840 bp 3.2 Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion ... produce VP2 VLPs of infectious bursal disease virus [19], and parvoviruses including PPV [14] and B19 [15] To further facilitate the use of PPV VP2 for diagnosis and vaccination, the goal of the current...

Ngày tải lên: 11/08/2014, 21:21

... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... with immobilized asialofetuin Five mg of asialofetuin were diluted in 0.1 M NaHCO3 and 0.5 M NaCl buffer, pH 8.3 and the solution was employed for immobilization of asialofetuin in Cyanogen bromideactivated-sepharose ... weight mass of the predicted translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was...

Ngày tải lên: 12/08/2014, 04:21

... between the upper band and lower band was measured using the molecular weight ladder as the standard curve, and it was found that the shift corresponded to a loss of the portion of the protein sequence ... broad overview of the immune response and inflammation to illustrate the importance of NF-κB and the far reaching effects of its regulation Also the introduction will focus on some of the biotechnological ... phenotypic effects of a complete loss of SIMPL protein and further understand its function, a KO strain of mice was produced using gene trapping technology Gene trapping is a high throughput and thus cost...

Ngày tải lên: 24/08/2014, 12:43

... chitinase caused a great loss of activity (Andersen et al., 1997; Iseli-Gamboni et al., 1998) In addition, mutation of Tyr 123 of a Zea mays chitinase and a similar tyrosine of an Arabidopsis chitinase ... rearranged after freezing and thawing, which led to the exposure of new Ca++-binding sites Binding of Ca++ to these sites caused inhibition of the antifreeze and chitinase activities of these complexes ... activity of TLPs: destabilizing target membrane on one hand, binding and/ or hydrolyzing fungal β-1,3-glucan on the other hand 1.3.3 TLP Gene Structure and Regulation A large number of cDNAs,...

Ngày tải lên: 13/11/2014, 09:25

... Abstract i CONTENTS ii INTRODUCTION MATERIALS AND METHODS 2.1 Materials 2.2 Methods 2.2.1 Preparation of fungi strain and culture medium 2.2.2 Extraction of the crude enzyme phytase 2.2.3 Phytase ... years because of the interest in such enzymes for reducing the levels of inorganic as well as organic phosphate pollution in livestock areas, and also improving nutritional values of organic phosphate ... 2.2 Methods 2.2.1 Preparation of fungi strain and culture medium Aspergillus fumigatus was maintained in PGA medium and incubated in 45°C for days Determine the centration of A fumigatus spore...

Ngày tải lên: 06/10/2015, 12:59

... mixture of chloroform-methanol and then re-extracted into hexane Menaquinones and ubiquinones contained in the crude extract were separated and purified using Sep-Pak® Plus Silica The type and concentration ... abbreviations of the type of quinone are ubiquinone: UQ, menaquinone: MK, plastoquinone: PQ and vitamin K1: K1 RESULTS AND DISCUSSION Diurnal variation of DOC concentration The diurnal variation of DOC ... variation of NH4-N concentration in pore water of tidal flat sediments with and without clams Biodegradability and molecular weight distribution of organic matter Figure shows that more than 80% of...

Ngày tải lên: 05/09/2013, 09:08