preparation of hydrogel substrates with tunable

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Ngày tải lên: 22/06/2014, 00:20

... duration of activation and the increase of fluorescence in- tensity inside of the nuclei of DU145 cells. Figure 2 visualizes the degree of the DNA damage in nuclei as a result of DNA strand ... (red – of the fusion protein KRED-Lamin B1 and green – derived from dNTP functionalized with ATTO 633) are hardly detectable inside of the nuclei transfected with pKillerRed-Lamin B1 without ... control. Illumination of the nuclei Before illumination, the solution of nuclei (410 6 ) was pipetted in a concentration of 410 6 nu- clei pro ml onto Petri dishes with a glass bottom. The...

Ngày tải lên: 25/10/2012, 11:15

... compliance with the guide for the care and use of laboratory animals from the Ministry of Science and Technology of the People’s Republic of China. Instruments HIFU ablation was performed with ... treatment of prostate hypertrophy. In 1956, HIFU was first introduced to the treatment of human solid car- cinomas [4]. The treatment of hepatocellular carci- n o m a ( H C C ) with HIFU was ... (for treatment of tumors of the liver, kidney, breast, uterus, pancreas and bone) or in the rectum (for treatment of the pros- tate), and are designed to enable rapid heating of a target tissue...

Ngày tải lên: 25/10/2012, 11:18

... resolution of the OAF, optimal management of complications, including patient discomfort, and good regeneration of soft tissues. Clinical significance: The principal advantage of the use of autologous ... reconstruction of soft tissues and to assure the preservation of the bone graft from epithelial connection. Conclusions: This surgical procedure showed a good stability of the bone grafts, with a complete ... elevation of the sinus membrane without disruption impossible 2 . Clinical Significance The principal advantage of the technique de- scribed here is the use of autologous bone graft with an...

Ngày tải lên: 25/10/2012, 11:48

... phenol-chloroform DNA extraction pro- tocol. TaqMan PCR analysis revealed that human serum of patients with acute coronary syndrome may contain genetic markers of C. pneumoniae with bacterial ... bodies of C. pneumoniae with typical electron microscopic structure can be isolated from the serum samples of the patients with acute coronary syn- drome. Furthermore, using combination of bacterio- logical ... relevant levels of IgG for C. pneumoniae were found in 34.2% of the patients with ACS, whereas IgA positive were seen just in 19.7% of the ACS patients. Simultaneous detection of increased...

Ngày tải lên: 26/10/2012, 08:57

... successful coupling of the RGD to the surface of the liposomes. Assay of particle sizes and morphological ob- servation of RGD-M-LCL The average size of liposomes processed by the microfluidizer was ... toxicity of RGD-LCL without matrine on cells, Bcap-37 cells were also treated with RGD-LCL of different concentrations (0.3125 mg/mL, 0.625 mg/mL, 1.25 mg/mL, and 2.5 mg/mL), and RGD-M-LCL of different ... Lyophilization of RGD-M-LCL and measurement of its particle size RGD-M-LCL was freeze-dried by a cryoprotec- tant of sugar in a sugar-lipid quality ratio of 2.0 (22), then was redissolved with DMEM....

Ngày tải lên: 26/10/2012, 08:57

... principal mechanism of action of all NSAIDs is the inhibition of the biosynthesis of prostaglandins by blocking the activity (selectively and/or non-selectively) of two isoforms of cyclooxygenase ... grade. Preparation of CD Solutions An aqueous solution of each CD was prepared by dissolving accurately weighed CDs (180 mg of β-CD and 300 mg of HP-β-CD) in 10 ml of distilled water with the ... and COX-2) [6-8]. With the exception of aspirin, NSAIDs inhibit synthesis of prostaglandins (mediators of inflammation) via competition with arachidonic acid for the two isoforms. Generally,...

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Ngày tải lên: 05/09/2013, 09:08

... RefreshIndividual() 1.6 Take Care of Error Handling with Bound Controls When dealing with database tasks, you are going to get runtime errors. In .NET, we call the various types of errors you can get exceptions. ... it so errors are handled gracefully within the application using bound controls? Technique Error handling is one of the most important aspects of working with data, between when a user is ... the Click event of the command button called btnSave. You will surround the SaveRecord routine call with a Try Catch End Try block. If an exception occurs, the Show method of the MessageBox...

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Ngày tải lên: 02/11/2013, 13:11

... your results with the instructor before proceeding. Dissolve 0.010 mole of sulfanilic acid (anhydride) in about 50 ml of a solution of sodium carbonate containing 0.010 to 0.0125 moles of sodium ... weigh the quantities of solid reagent very carefully to the accuracy of 0.05 g or better. In this experiment you will have to calculate for yourself some of the amounts of needed reagents. After ... solution of N,N-dimethylaniline (0.010 mol) in 0.010 mol of acetic acid in a 25 ml Erlenmeyer flask. Now add the dimethylaniline acetate solution slowly with constant stirring to the suspension of...

Ngày tải lên: 12/12/2013, 21:16

... circuits with hundreds of gates on a chip. These chips were called MSI (Medium Scale Integration) chips. With the advent of LSI (Large Scale Integration), designers could put thousands of gates ... program will not return a value. The sizeof operator used in the loop for i returns the size of the array a in bytes. For this case sizeof(a)=28 sizeof(int)=4 The cout statement in C++ is used ... sake of simplicity, in this book, we will refer to all design tools as EDA tools. With the advent of VLSI (Very Large Scale Integration) technology, designers could design single chips with...

Ngày tải lên: 21/01/2014, 17:20

... process is done with the assistance of EDA tools. Logic synthesis tools convert the RTL description to a gate-level netlist. A gate-level netlist is a description of the circuit in terms of gates ... to analyze the design in terms of functionality, performance, compliance to standards, and other high-level issues. Behavioral descriptions are often written with HDLs. [2] [2] New EDA tools ... probability of hitting a functional bug at a later time in the gate-level netlist or physical layout is minimized. ã Designing with HDLs is analogous to computer programming. A textual description with...

Ngày tải lên: 21/01/2014, 17:20

... parts of a design. N ew verification languages have also gained rapid acceptance. These languages combine the parallelism and hardware constructs from HDLs with the object oriented nature of ... user to write custom C code to interact with the internal data structures of Verilog. Designers can customize a Verilog HDL simulator to their needs with the PLI. [ Team LiB ]  [ Team ... speed and complexity of digital circuits have increased rapidly. Designers have responded by designing at higher levels of abstraction. Designers have to think only in terms of functionality....

Ngày tải lên: 21/01/2014, 17:20