predesigned three dimensional self assembly of proteins crystal engineering

... the design of protein vectors that are capable of self- assembly with nucleic acids The key principle behind our design of the protein vectors is the use of nucleic acids which encode proteins that ... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... SynGene The results of measurements are presented on Figure Self- assembly of protein vectors with their DNAs and affinity separation In vitro synthesis and self- assembly of the SAPV-84, SAPVAlb5...

Ngày tải lên: 11/08/2014, 00:21

... the promiscuity of S100 proteins Therefore, systematic studies of the conformational changes and oligomerization of S100 proteins will be of critical importance in the development of potential therapeutics ... et al Natural and amyloid self- assembly of S100 proteins Amyloid formation by S100A8 ⁄ A9 proteins Recently, we have found a new amyloidogenic property of S100A8 ⁄ A9 proteins, implicating them ... Natural and amyloid self- assembly of S100 proteins A Ca2+ G Fritz et al C E S100B HII HI S-100 EF-hand F S100A12 Ca2+ B D 90° HIV G HIII S100A8/A9 EF-hand Fig Structure of S100 proteins (A,B) Calcium-driven...

Ngày tải lên: 23/03/2014, 03:20

... periodicity of 1.26 nm are clearly visible in STM images of the h-BN phase (The image was taken with a bias voltage of VB = 1.8 V and a set tunneling current of IT = nA.) The corrugation in STM images of ... for a variety of molecules [26] Since changing the preparation temperature allows to tune precisely the relative weights of the two phases, one can control the process of self- assembly, which ... narrower In summary, we report the discovery of a tunable system of two self- assembling nanostructures The structures are very appealing because of their 1D characters and their directional orthogonality...

Ngày tải lên: 22/06/2014, 22:20

... 1.5.2 Applications of self- assembled technique 35  1.5.3 Self- assembly of thiolated cyclodextrins 37  1.6 Objectives 38  Chapter 2    Perfunctionalization of -cyclodextrins ... configuration/view of quartz crystal microbalances with a holder and connector 28  Figure 1.10 AT-cut of a quartz crystal from which the metal coated QCM quartz crystals are produced and an end on crystal ... sections of this chapter, approaches for chiral discrimination and separation, working mechanism of quartz crystal microbalance, preparation of perfunctionalized -cyclodextrins and self- assembly...

Ngày tải lên: 11/09/2015, 10:15

... Templated self- assembly of porous alumina: Phenomenology Experimental Details Results and discussion 6.4.1 Templated self- assembly by patterning the underlying substrate 6.4.2 Templated self- assembly ... formation Other factors affecting stability of the AllAAO interface and the self- assembly process are also presented Second, templated self- assembly (TSA) of AAO pores, ordered over wafer-scale areas ... Effect of the wavelength of a perturbation on its growth rate For templated self- assembly, voltages that correspond to the fastest growing wavelength equal to D1 and D2 still lead to self- assembly...

Ngày tải lên: 20/03/2014, 13:11

... Mn-doped ZnO HMSs in view of morphology transformation A lot of EG molecules are adsorbed on the (001) surface of the ZnO crystals and block the crystal growth along both sides of the c-axis Apart ... development of a more controllable and repeatable synthetic route for RTFM Mn-doped ZnO nano/microstructures is crucial to their practical applications The fabrication of hierarchical and self- assembly ... topic in recent material research fields [14-16] Self- assembly and/or self- aggregation are fundamental mechanisms by which different nanoparticle assembly motifs or even close-packed periodic structures...

Ngày tải lên: 20/06/2014, 20:20

... Mn-doped ZnO HMSs in view of morphology transformation A lot of EG molecules are adsorbed on the (001) surface of the ZnO crystals and block the crystal growth along both sides of the c-axis Apart ... development of a more controllable and repeatable synthetic route for RTFM Mn-doped ZnO nano/microstructures is crucial to their practical applications The fabrication of hierarchical and self- assembly ... topic in recent material research fields [14-16] Self- assembly and/or self- aggregation are fundamental mechanisms by which different nanoparticle assembly motifs or even close-packed periodic structures...

Ngày tải lên: 21/06/2014, 17:20

... nonoverlapping trees of all the coefficients of the wavelet transform of the image The tree structure is illustrated in Figure for three levels of decomposition in each direction Each of the coefficients ... different parts of the image, enabling the possibility of high-quality region of interest (ROI) and the possibility of discarding unused portions of the image An unused portion of the image could ... modifications are required The problem of error resilience is developed in [15] on a block-based version of 3D-SPIHT A general review of these modifications and a comparison of performances is provided in...

Ngày tải lên: 22/06/2014, 00:20

... nanostructure like ‘‘cigarette’’ were synthesized by self- assembly of the multiple layers of nanoparticles on the top of the nanowires The incorporation of self- assembled nanoparticles between the nanowire ... Nanoscale Res Lett (2007) 2:34–39 pores of membrane Therefore, the chemical modification spatially is realizable on the exposed top of nanowires by self- assembly method Mallouk et al have prepared ... aluminium substrate and obtain the Au/ Cu/AAO membrane Fabrication of nano ‘‘Cigarette’’ by self- assembly The site-specific anchoring of nanoparticles on nanowires to form the novel structures-nano...

Ngày tải lên: 22/06/2014, 22:20

... induction of selfassembly of ensembles by interaction of silver nanoparticles produce a highly ordered replica of uniform nanostructures The most prominent systems have been triblock copolymers of the ... the fascinating self- assembly Scheme Schematic illustration of large-scale nanostructuring-fabrication with a triblock copolymer 123 Nanoscale Res Lett (2007) 2:119–122 behaviour of amphiphilic ... to Fig Formation of nanostructured micelles, aggregates and vesicles in the mixture of cubic and hexagonal phases of EO20PO70EO20 induced by silver nanoparticles TEM micrograph of vesicles and...

Ngày tải lên: 22/06/2014, 22:20

... With the use of unenhanced scan images, the HU values of the AO, CVC, PV, and liver were measured in the determined cine scan location (Table 1) Regions of interest (ROI) were Three- dimensional ... scan images, the region of interest (ROI) was set up in the center of the aorta, and then a time-attenuation graph was constructed from the ROI use of cine CT; 2) the use of a retrospective reconstruction ... consuming Ultrasound is an expedient method for imaging of the hepatic vasculature, but disadvantages Three- dimensional CT angiography of the canine hepatic vasculature 411 Fig Average pixel...

Ngày tải lên: 07/08/2014, 23:22

... performed with µL of 30 ng/µL of genomic template DNA, µL of µmol/L each of HEX-HFE-2F primer and H63DR primer (Table 1), µL each of 10 mmol/L dNTPs, 0.75 µL of 50 mmol/L of MgCl2, 2.5 µL of 10× PCR ... rapid investigation of self- assembly mechanisms In addition, this re-hybridisation enables the formation of duplexes made from a sample and a set of DNA references – i.e DNA self- assembly within ... expect then that the sensitivity of the combined methods will be close to 100% We are now applying this method as part of a study of the application of DNA self- assembly based mutation detection...

Ngày tải lên: 11/08/2014, 00:22

... nanofibers: a versatile scaffold for the preparation of self- assembling materials Proc Natl Acad Sci 2002, 99:5133-5138 Hartgerink JD, Beniash E, Stupp SI: Self- assembly and mineralization of ... for hrs This type of turbidity was not seen in PBS or phosphate-citrate buffer This Figure Solubility profile of AF-1 in varying pH and buffer conditions Solubility profile of AF-1 in varying ... the presence of rodlike and worm-like structures were seen in cryo-TEM micrographs (data not shown) Studies on the self assembly of certain surfactants have described the interplay of theoretical...

Ngày tải lên: 11/08/2014, 00:22

... surface three- dimensional reconstruction (of the coronal plane) correspond with bottom right picture, and is framed with a white arrow (instead of white pixel) tion demonstrated the presence of active ... pixel (placed in the center of each image) always correspond with the same space point of the three orthogonal planes, and is located referring HM, between UT (at the level of the uterine scar) and ... scan (in multiplane acquisition mode) of the uterus-hematoma-bladder complex Three- dimensional transvaginal ultrasound scan (in multiplane acquisition mode) of the uterus-hematomabladder complex...

Ngày tải lên: 11/08/2014, 19:21

... Only the deletion of the whole NLS domain may allow for the expression of a large amount of the Cap protein However, the NLS plays an important role forming the self assembly of Cap-like particles ... The reaction of Cap-tag and nCap may with anti-PCV2 pig sera and was observed by Western blotting (Fig 3) Cleavage of His-Smt3 tag and assembly of Cap protein VLPs After the cleavage of the His-Smt3 ... virus-like particles of hepatitis E virus J Virol 2005, 20:12999-13006 doi:10.1186/1743-422X-7-166 Cite this article as: Yin et al.: Self- assembly of virus-like particles of porcine circovirus...

Ngày tải lên: 12/08/2014, 04:20

... conditions for LBL self- assembly 102 4.5.1.2 Nature of spacer layer: PEI vs PDAD 104 4.5.1.3 Effect of base on adsorption of 6F 106 4.5.1.4 Enhanced adsorption of 6F: Proposed mechanism ... hybridization by self- assembly of ex-situ hybridized PNA probe-target DNA duplex directly on charged surface The electrostatic self- assembly makes the strategy straightforward without any need of covalent ... in proteins 138 Table 6.1: Effect of solution pH on quantum yield of P3 P4, P5 153 Table 6.2: Effect of pH on aggregate size of P3¸ P4, P5 in solution 154 Table 6.3: Ksv values of...

Ngày tải lên: 14/09/2015, 08:38

... (SAMs) of molecules,7 molecular crystals,3a,8 phase separated polymers,9 and quaternary structure of proteins, 10 are a few examples of molecular self- assembly Self- assembly represent the most useful ... electron microscope (c, d) images of nano-rods formed by self- assembly of OLG1 in aqueous solution (THF: H2O) 147 Figure 5.8 XRD patterns of OLG1; powdered (▲) and self- assembly ( ) sample drop casted ... synthesis Self- assembly Figure 1.1 Schematic representations of nanostructure fabrication; lithography (a) and self- assembly (b) On the other hand, an enabling technique for nanotechnology, self- assembly...

Ngày tải lên: 14/09/2015, 14:02

... NBF4 /CH3 CN at scan rate of 50 mV s¡1 112 xv 5.1 Schematic diagram of self- assembled Au nanoparticles onto silanized ITO glass electrode and subsequent self- assembly of 5SHBT molecules (A: ... structures of thiophene 1.4 Formation of mid-gap states of polaron or bipolaron upon p-doping 1.5 Structures of or position functionalized oligothiophenes 1.6 Structures of thiophenes ... SYNTHESES AND SELF- ASSEMBLY OF NANOSTRUCTURE AS MODIFIED ELECTRODES FOR POLYTHIOPHENE PREPARATION ZHANG CHUNYAN (B Sc Nanjing University of P R China) A THESIS SUBMITTED FOR DOCTOR OF PHILOSOPHY...

Ngày tải lên: 17/09/2015, 17:17

... thickness of the films allows the use of common surface analytical techniques to determine the chemical state and composition of species at the metal surface 28 2.3.1 KINETICS OF SELF- ASSEMBLY Self- assembled ... observation of a higher S/Cu ratio with XPS analysis and a greater surface roughness with AFM microscopy analysis [55] 27 2.3 SELF- ASSEMBLY Self- assembly (SA) is described as the spontaneous formation of ... BOND 25 2.3 SELF- ASSEMBLY 28 2.3.1 KINETICS OF SELF- ASSEMBLY 29 2.3 BUILDING MULTIPLE ORGANIC INHIBITOR LAYERS ON COPPER 31 2.4 REMOVAL OF ORGANIC FILM FROM METAL...

Ngày tải lên: 13/10/2015, 16:50

... is a registered trademark of Computers and Structures, Inc SAP2000 is a registered trademark of Computers and Structures, Inc Windows is a registered trademark of Microsoft Corporation Adobe and ... selection The Properties of Object pop-up box for frames will appear as shown in Figure 10 Figure 10 Properties of Object box If the Properties of Object box is covering any part of the model in either ... loads used in this tutorial consist of dead and live static loads acting in the gravity direction For this example, assume that the dead consists of the self weight of the bridge plus an additional...

Ngày tải lên: 06/09/2012, 15:56