precursors progenitors and stem cells in the adult cns

... detected in patients with PTB in this study may therefore indicate the contribution of NK cells in the host immune response against MTB infection The immune response after MTB infection and disease ... the patients in this study Immunity against MTB comprises of a predominant cellular response and majority of researchers have dismissed the participation of B cells in defending against the infection ... response against MTB by increasing the infection inoculum (Vordermeier et al., 1996; Maglione et al., 2007) In addition the protection of mice against MTB infection by administration of intravenous...

Ngày tải lên: 29/03/2014, 03:20

... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium ... primitive oocyte-like cells were released from their “chambers” in the ovarian surface epithelium and started to change into hypertrophic/necrotic cells (Figures and 6) and further into autofluorescent ... oocyte-like cells in the adult human ovarian surface epithelium as related to epithelial ovarian cancer These cells resembled primitive oocytes in the ovarian surface epithelium of fetal ovaries, and...

Ngày tải lên: 20/06/2014, 08:20

... line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the CIA mice The short-term presence of UC-MSCs in the spleen ... proinflammatory cytokine IL-6 Interestingly, the UC-MSCs could downregulate the IL-6 production in the transwell but not the cell-to-cell contact system (Figure 3c) both in single time point and in dynamic ... CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition, MCP-1 is a member of the CC family and could be induced by inflammatory...

Ngày tải lên: 12/08/2014, 15:22

... into an oocyte or fused with an ES cell, the underlying mechanisms remain obscure Using the ES fusion system, Smith has found an unexpected role for the homeodomain-containing protein Nanog in ... germ cells and ES cells is their epigenetic imprint status Shinohara reported that while the spermatagonial cells retain a stable androgenic imprint over long-term culture, the pluripotent cells ... by our inability to derive rat ES cells Shinohara hinted that he was making progress in applying his findings to the rat Finally, Tariq Enver (University of Oxford, UK) described a mathematical...

Ngày tải lên: 14/08/2014, 16:21

... temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma) for minutes and counterstained ... The GAG content reflected proteoglycan biosynthesis and accumulation in the matrix, and under hypoxic conditions there was a 1.9-fold increase in the total GAG per aggregate In spite of the increased ... antibody (green), and 4',6-diamidino-2-phenylindole (blue) Results showed strong staining for CD13, CD29, CD44, CD90 and CD105, weak staining for 3G5, and negative staining for low-affinity nerve growth...

Ngày tải lên: 09/08/2014, 10:20

... staining predominantly the tunica adventitia consisting of supporting tissue in the vasculature, vWF (c, d) staining predominantly the tunica intima consisting of the endothelial layer and the ... CD90, and CD105, weak staining for 3G5, and negative staining for LNGFR, STRO1, CD34, CD56, and the IgG control The FGF-2-expanded cells are morphologically different from cells expanded in the ... doublings These cells retained cell surface staining similar to the original parent population, with consistent staining for the various markers identified above (data not shown) The staining...

Ngày tải lên: 09/08/2014, 10:23

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... PJ, Wu JE, Santoro SA 1995 ;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis ... 1978 ;The relationship between the spleen colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885...

Ngày tải lên: 11/09/2015, 09:16

... (Kim et al., 1996) The first DNA-binding domain used was a fruit fly homeobox domain, followed by zinc-finger DNA binding domain and yeast Gal4 DNA-binding 17 domain (Kim and Chandrasegaran, 1994; ... developed a system of adenosine-releasing fibroblasts with inactivated adenosine kinase and adenosine deaminase These cells were transplanted into brain ventricles in a rat epilepsy model and were ... fusion proteins of a DNA-binding zinc finger domain and a double strand break-inducing nuclease domain Dimerization of nuclease domains is necessary for target cleavage The cellular machinery for...

Ngày tải lên: 26/11/2015, 09:54

... pyramidal cells of the cortex and in the hilus of the dentate gyrus, Purkinje cells and granule cells in the cerebellum Scale bar, 45 lm Fig In situ hybridization showing an ADH3 specific signal in the ... involvement of ADH1 and ADH4 in ethanol oxidation in brain tissue Regarding retinoid metabolism in the adult brain, enzymes other than ADH4 must be active, because in vivo and in vitro data indicate ... can be oxidized in brain homogenates and that catalase is involved in the accumulation of acetaldehyde in the brain [27], explaining its presence despite the absence of ADH1 and the fact that acetaldehyde...

Ngày tải lên: 21/02/2014, 00:20

... cap structure at the 50 end of the mRNA, involved in 30 UTR processing, splicing, and mRNA transport into the cytoplasm, has yet another role in translation by binding the cap-binding complex (Fig ... ball, which introduces the clinical and the ethical views of the gamete generation from stem cells, probably one of the main key points of the stem cell field in reproductive medicine, is by two ... paracrine and endocrine factors and cell-cell interaction This interaction is initiated once PGCs enter the genital ridges during their homing period and is continued until fertilization PGCs and the...

Ngày tải lên: 22/03/2014, 20:21

... capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated by the Ficoll-Hypaque ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly...

Ngày tải lên: 18/06/2014, 16:20

... during pyrolysis, the passing on an inert gas, such as N2 or Ar, favors the development in the carbon's porosity In this case, the flow and the gas type may affect the final properties of the ... number of works increased in the years from 2000 to 2010 The obtained carbons were mainly employed in the removal of water pollutants In the present chapter the principal methods used in the preparation ... by the weight of the original carbon on dry basis according with the following equation, (1) where W0 is the weight of the original carbon and W1 refers to the mass of the activated carbon The...

Ngày tải lên: 28/06/2014, 17:20

... of the endocrine cells within the pancreas, intestine, and stomach of the gar Gen Comp Endocrinol 1997, 106, 1-16 Guyot, E., Diaz, J P., Romestand, B and Connes, R Insulin during the early postembryonic ... PP-immunoreactive cells Insulin is synthesized in the B cells of the pancreatic islets and regulates the serum glucose levels (Hsu and Crump, 1989) The regional distribution and relative frequency of the insulin-immunoreactive ... chromogranin containing cells in the porcine gastroenteropancreatic endocrine system Jpn J Vet Sci 1987, 50, 395-404 11 Jansson, L and Sandler, S L The influence of cyclosporin A on the vascular...

Ngày tải lên: 07/08/2014, 14:23

... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht ... enircodne ni A ninargomorhc fo noitartsnomed lacimehcotsihonummI T aganawI ,N arahianaY ,F otaS ,H ikhO ,Y otomihsaH 01 892-782 ,25 ,9891 lotyC lotsiH hcrA saercnap enircodne eninac eht ni II ninargoterces ... yltnacifingis a ,noitidda nI ]5[ esaesid s’nhorC dna ]5[ sitiloc evitareclu ,]41[ romut ,]61[ noitapitsnoc cinorhc htiw stneitap fo mutcer eht ni desaercni osla yeht dna ]43[ asocum cinoloc ni...

Ngày tải lên: 07/08/2014, 18:21

... viability in vitro and in vivo [4,12,39], but there are few reports on autologous SCNT in pigs The objective of the present study was to examine the feasibility of producing autologous porcine SCNT ... derived from adult skin fibroblasts The data were analyzed using the general linear model procedure in the Statistical Analysis System version 9.1 software (SAS Institute, USA), followed by the least ... cell cycle is an important factor in the development of SCNT embryos [6,38] Cells in the G0/G1 phases are superior to cells in other phases of the cycle in terms of their ability to support NT embryo...

Ngày tải lên: 07/08/2014, 20:23

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using the ... points post-SCI, at the start of treatment The scores for the ASC group increased to 1, 3.6 and 4.6 points at 3, and weeks, respectively (Fig 4) Scores in the C and V groups remained below point ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells...

Ngày tải lên: 07/08/2014, 23:22

... proliferation and the cell cycle progression in nucleus pulposus cells, (2) determine if c-Myc transcription inhibitor obstructed the effect of TGF1, and (3) determine the role of ERK1/2 in stabilizing the ... treatment The cell cycle distribution in TGF1treated cells (Figure 5b) indicates a large increase in cells in the S phase, associated with the suppression of p21 and p27 which belong to the Cip/Kip ... Competing interests The authors declare that they have no competing interests 14 Authors' contributions TN and DS conceived of the study and performed the experimental work DS and JM participate in...

Ngày tải lên: 09/08/2014, 01:22

... when the body is threatened by infection or injury In this setting T cells will become activated, and will recruit other T cells and other inflammatory cells and mediators to the site As the response ... fate of the TR cells induced at the site is not known — whether these cells exist only transiently then die, whether they persist in the body as TR cells, or whether they return to their previous ... involution, and antigen exposures, the development of CD4+CD25+ TR cells may occur in the periphery in man Induction of CD4+CD25+ TR cells in the periphery The induction of TR cells that resemble the...

Ngày tải lên: 09/08/2014, 01:24

... NK and THP-1 cells, the role of IL-15 and IFN-γ (a) IL-15 and β2 integrins are involved in the intercellular contact with and THP-1 cells, the role of IL-15 and IFN-γ THP-1 cells that induces the ... supporting the importance of intercellular contacts in the pathogenic mechanisms underlying RA Indeed, the importance of these cell-cell interactions in the synthesis and release of pro-inflammatory ... CD48, than in monocytes that express both CD48 and CD244 Thus, our findings show that NK cells can engage and stimulate monocytic cells, resulting in the synthesis of TNF This finding opens the possibility...

Ngày tải lên: 09/08/2014, 08:22

... saline for 20 minutes and stored in 70% ethanol until processed for paraffin embedding Proteoglycan production in the ECM was visualised by toluidine blue staining (Sigma, St Louis, MO; 0.1% in ... and assayed for sulphated proteoglycan production using the DMB assay [26] Scoring of immunohistochemistry and toluidine blue staining Scoring of slides from immunohistochemical staining of cellsurface ... capable of differentiating into adipocytes, chondrocytes and osteoblasts, and, more recently, have been shown to differentiate into insulin-, somatostatinand glucagon-expressing cells [13] AD-MSC...

Ngày tải lên: 09/08/2014, 10:23