postmortem lung biopsy for obtaining lung tissue in congenital diaphragmatic hernia
Long term comparison of four techniques for obtaining nasal symmetry in unilateral complete cleft lip patients a single surgeons experience
... nasal webbing, obvious cleft ala deformity); (2) poor; (3) fair (oval with indentation); (4) Volume 126, Number • Techniques in Primary Cleft Rhinoplasty Fig Postoperative nasal retainer for group ... nasoalveolar molding alone without primary rhinoplasty It would appear that if a surgeon did not perform primary rhinoplasty for various reasons, nasoalveolar molding alone could obtain results similar ... Cutting CB Presurgical nasoalveolar molding in infants with cleft lip and palate Cleft Palate Craniofac J 1999;36:486–498 Grayson BH, Cutting CB Presurgical nasoalveolar orthopedic molding in...
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Novel method for sputum induction using the Lung Flute in patients with suspected pulmonary tuberculosis pdf
... use of the Lung Flute included mild sore throat after blowing into the device in four patients (12%) and hyperventilationrelated symptoms in three patients (9%), including dizziness in two (6%), ... the clinical use of the Lung Flute in diagnosis of TB The device may represent a new technology for sputum induction for the diagnosis of pulmonary TB The Lung Flute for sputum induction was invented ... recommended in patients suspected of having pulmonary TB.13 But, most patients feel discomfort of throat during the inhalation of irritant hypertonic saline In clinical practice, single induced sputum...
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Báo cáo y học: "Proteinase-3 as the major autoantigen of c-ANCA is strongly expressed in lung tissue of patients with Wegener’s granulomatosis" pptx
... little-known issue, we examined the PR-3 expression in normal lung and lung tissue of WG patients, as the lung is the most frequent organ involved in WG [15] In normal lung tissue, mainly pneumocytes type ... PR-3 mRNA increase compared with normal lung tissue Similar to our findings in normal lung tissue, we could demonstrate in lung tissue of WG patients that PR-3 is mostly expressed in pneumocytes ... size for PR-3 mRNA), especially in lung tissue We found just a very weak signal in the heart and brain, and could not detect a band in liver tissue (Supplementary Fig 1) In situ hybridization for...
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Báo cáo khoa học: "Recommendations for implementing stereotactic radiotherapy in peripheral stage IA non-small cell lung cancer: report from the Quality Assurance Working Party of the randomised phase III ROSEL study" pps
... motion into account However, similar to the reasoning given for the ITV to PTV margin, a minimum margin of mm should be used for the incorporation of the other uncertainties Organs at risk definition ... definition of internal target volumes using slow CT alone or in combination with thin-slice CT under breath-holding conditions during the planning of stereotactic radiotherapy for lung cancer Radiother ... the lung receiving more than 20 Gy (Figure 5), it is clear that a type B algorithm will result in higher values, due to the fact that the change in lateral scattering in lung tissue is taken into...
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Báo cáo y học: " Bronchoalveoloar lavage fluid cytokines and chemokines as markers and predictors for the outcome of interstitial lung disease in systemic sclerosis patients" pps
... severity was taken into account [3] The authors concluded that BAL findings add only limited prognostic information in SSc-related interstitial lung disease in addition to HR-CT scans and lung- function ... CCL3, were increased in idiopathic ILD as well as in SSc-associated ILD, indicating an important, but nonspecific contribution of these chemokines in lung fibrosis As tested here for SSc patients, ... Effect of IL2-Bax, a novel interleukin-2-receptor-targeted chimeric protein, on bleomycin lung injury Int J Exp Pathol 2005, 86:279-288 39 Sharma SK, MacLean JA, Pinto C, Kradin RL: The effect of an...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
... allowed for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids ... supporting the proteomic results Notably, nine cases of LCNEC including four used for the proteomic experiments Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 ... in LCC had small areas with neuroendocrine tendency in the tissue Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 structure as pointed...
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Báo cáo y học: " Anaesthesia for serial whole-lung lavage in a patient with severe pulmonary alveolar proteinosis: a case report" pdf
... WLL was performed with the patient in the supine position on the operating table One -lung ventilation of the left lung was commenced just before initiation of lavage of the right lung Under fibreoptic ... tidal volume during one -lung ventilation is crucial to detect fluid leakage into the ventilated lung An increase in airway pressure or decrease in tidal volume may indicate a reduction in compliance ... Anaesthesia for WLL is undoubtedly hazardous: the use of one -lung ventilation for broncho-alveolar instillation and drainage of large volumes of fluid in the setting of pre-existing respiratory...
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Báo cáo khoa học: " A new therapeutic strategy for lung tissue injury induced by influenza with CR2 targeting complement inhibitior" ppt
... above studies indicate that through inhibiting complement activation, excessive inflammatory reaction in lung tissues induced by influenza can be inhibited, and as a result, lung tissue injury can ... activation by inhibite the activity of C3/C5 convertase Influenza viral lung injury and complement activation Many studies have proved that excessive inflammatory injury in lung tissues induced by influenza ... will induce severe inflammation, and results in indirect hazard, which may induce inflammation again, damage the lung, and finally result in fatal pneumonia and acute respiratory tract infection...
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Báo cáo y học: "Iron deposition and increased alveolar septal capillary density in nonfibrotic lung tissue are associated with " ppt
... PH or indicators for further clinical work-up in IPF patients, mainly using the sections of surgical lung biopsies that had been performed for the diagnosis It has been thought that PH in IPF ... angiogenesis in nonfibrotic lung tissues [19-21] A greater angiogenic response to IL-8 was detected in UIP lung tissue than in control tissue [22] A study shows that TGF-beta1-induced and hypoxia-induced ... remaining gross specimens stored in the surgical pathology files for a complete microscopic examination of existing tissues for all surgical biopsies We also thoroughly examined explanted lungs...
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Báo cáo y học: "Cigarette smoke promotes dendritic cell accumulation in COPD; a Lung Tissue Research Consortium study" pdf
... COPD lung tissue procured through the Lung Tissue Research Consortium (LTRC) In addition, complimentary studies were conducted using relevant in vitro human and in vivo murine models to determine ... was quenched by placing slides in a 1% hydrogen peroxide solution in methanol for 30 minutes Tissue slides were then washed (1× Dako Wash Buffer) and immunostaining performed using a commercially ... and analyzed for HO-1 and Bcl-xL protein using relevant antibodies as described in methods β-Actin protein levels were determined as an internal control to ensure equal protein loading Representative...
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Báo cáo y học: " Deregulation of apoptosis mediators'''' p53 and bcl2 in lung tissue of COPD patients" ppt
... Immunostaining for p53 and bcl2 was carried out using standardized protocols Tissue samples were fixed in 10% formalin and embedded in paraffin μm thick serial tissue sections, were obtained and ... b-actin, which was used as internal control, was performed using standard protocols In detail, lung tissue specimens from all subjects were homogenised in order to obtain the corresponding protein ... immunostaining, additional tissue blocks were fixed in 10% formalin for at least 24 hours After fixation, each tissue block was embedded in paraffin and sections μm thick were cut following routine procedures...
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Báo cáo y học: " Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease" docx
... albumin Human serum albumin (HSA) is a single nonglycosylated polypeptide containing 35 cysteine residues all involved in the formation of stabilising disulphide bonds except 34cysteine In plasma, ... baseline levels of HSA Human lung tissue from 38 individuals classified using the GOLD guidelines was homogenised and adjusted for total protein HSA levels were measured in lung homogenates using ... 14.0 for Windows (SPSS, Chicago, Illinois, USA), data are given in Table For the purposes of this study ex-smokers were defined as that had given up smoking for ≥3 years to ensure for smoking...
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Báo cáo y học: " Elemental analysis of lung tissue particles and intracellular iron content of alveolar macrophages in pulmonary alveolar proteinosis" ppt
... by employing in- air microparticle induced X-ray emission (in- air micro-PIXE) analysis for particles and intracellular iron in lung tissue specimens combined with Berlin blue staining for iron ... of normal lung of the margin of tumor was used for the analysis Tissues were subjected to in- air micro-PIXE analysis and Berlin blue staining for iron In- air micro-PIXE analysis For in- air micro-PIXE ... morphology indicated by P surronded by S-containing surfactant Serial sections of lung tissue with Berlin blue staining showed iron-rich alveolar macrophages In contrast, intracellular Fe in a macrophage...
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Báo cáo y học: "Immunodetection of occult eosinophils in lung tissue biopsies may help predict survival in acute lung injury" pdf
... staining of lung tissue sections H&E staining was performed using an automated stain processing unit in the Mayo Clinic Arizona clinical histology unit Immunohistochemistry was performed using ... staining with EPX-mAb and slide served as an isotype immunoglobulin negative control for the immunohistochemistry The eosinophil infiltration of lung tissue using H&E staining was performed in ... immunohistochemistry was independently performed in an intra/inter-blinded fashion that included investigators from pathology (KL, EJ, and PG - EPX-mAb based eosinophil counts in lung tissue) ; KL and...
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Báo cáo y học: "Interstitial lung disease in connective tissue diseases: evolving concepts of pathogenesis and management" potx
... connective tissue diseaseassociated interstitial lung diseases Mediators of lung fibrosis in systemic sclerosis Interstitial lung involvement in SSc develops from an interplay of autoimmunity, in ammation, ... activation induced by insulin-like growth factor (IGF) binding protein [25] These observations identify Egr-1 as a critical intracellular mediator of lung fibrosis in humans and in mouse models Ongoing ... ineffective Stem cell therapies and novel agents including rituximab, angiotensin II inhibitors, tyrosine kinase inhibitors, PPARγ agonists, intravenous immunoglobulin, and biologicals targeting...
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Báo cáo y học: " Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection" ppt
... cells in normal lung tissues, hBD-4 immunostaining in lung tissue of chronic LRTI was observed in the bronchial lumen as well as in the cytoplasm of epithelial cells Also, IHC and ELF findings ... hBD-4 in lung Normal human lung tissue, isolated as described above for immunohistochemical studies, was heated at 95– 100°C for 10 in a 10-fold volume of water to inactivate intrinsic proteinases ... proteinase K (DakoCytomation) for 30 at 37°C and treated with 6% hydrogen peroxidase for 60 to inactivate endogenous peroxidases Nonspecific binding was inhibited by an incubation in Protein Block...
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Báo cáo y học: " CD8+ T lymphocytes in lung tissue from patients with idiopathic pulmonary fibrosis" pot
... dark brown color with counterstained cells as false blue Formal scoring (labeling Index) for each antibody was then performed in one section for each paraffin block Interobserver variability was ... = 0.02) most prominent and disabling symptom in these patients This study shows that the type of the inflammatory infiltrate in lung tissue of patients with UIP/IPF was predominantly mononuclear, ... parenchyma still remain unknown and further studies are needed for their clarification The increase in CD8+ TLs observed in lung surgical biopsies in patients with IPF appears intriguing Classically,...
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Báo cáo y học: "A role for MCP-1/CCR2 in interstitial lung disease in children" pps
... Proteins in Response to Interleukin-4 Journal of Clinical Investigation 1992, 90:1479-1485 Duncan MR, Berman B: Gamma-Interferon Is the Lymphokine and Beta-Interferon the Monokine Responsible for ... dramatic inhibition of leukocyte accumulation in murine lungs[17] Furthermore, CCR2-/mice are protected from fluorescein (FITC) or bleomycin induced lung fibrosis[18] Thus far, CCR2+ T cells in BALF ... during 1999–2004 were considered for inclusion in this study Children suspective of ILD underwent a comprehensive clinical evaluation, including patient history, physical examination, routine...
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Báo cáo y học: "Clinical risk conditions for acute lung injury in the intensive care unit and hospital ward: a prospective observational study" potx
... for the development of other clinical risk conditions Screening for ALI/ARDS diagnosis was continued for days unless another clinical insult developed, in which case follow-up was continued for ... knowledge in two ways First, our finding that most patients entering the ICU so on the day of developing their clinical insult is new, and it underscores the potential need for rapid intervention in ... risk for each clinical insult The frequency of ALI/ARDS also increased with the presence of an increasing number of clinical risk conditions (2.2%, 13.9%, and 20.8% for one, two, and three clinical...
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Báo cáo y học: "New and conventional strategies for lung recruitment in acute respiratory distress syndrome" pps
... recruitment maneuver In direct (pulmonary) lung injury, the primary structure damaged is the alveolar epithelium resulting in alveolar filling by edema, fibrin, and neutrophilic aggregates In indirect (extrapulmonary) ... areas increases, opening alveoli and improving gasexchange [22] Some authors have reported that in healthy [23], as well as in lung- injured animals [24], mechanical ventilation leading to lung ... abdominal contents and heart [22], yielding a redistribution of ventilation; (b) increased endexpiratory lung volume resulting in a reduction in stress and strain [25]; and (c) changes in regional...
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