... user locates the positions ofthe implanted markers From these positions the system reconstructs the 3D geometry ofthe implanted markers and determines the shifts necessary to bring them into alignment ... verify the accuracy ofthe planned dose calculation Due to these limitations, the treatment ofthe emergency patient on these systems generally requires performance ofthe standard workflow of CT ... near the target Two markers, cm away from each other and placed close enough to the target anatomy so that they could be observed within the field of view ofthe x-ray localization system at the...
... determining the cycle number at which the change in the fluorescence ofthe reporter dye (ΔRn) crossed the threshold To synchronize each experiment, the baseline was set automatically by the software ... 1.2.2 software to synchronize among experiments The threshold cycle (CT) was determined by the cycle number at which the change in the fluorescence ofthe reporter dye (delta Rn) crossed the threshold; ... showing the slope for Cb Page of reaction was -3.38 with R2 value of 0.9986 (Figure 1B) The slope and R value of standard curves for other reactions are listed in Table Specificity ofthe qRT-PCR...
... compare the results ofthe standard culture method of isolated field and reference strains with those ofthe multiplex real-time PCR Results Specificity ofthe multiplex real-time PCR A total of 128 ... were synthesized by a commercial company (Operon, Germany) The dual-labeled probes were prepared by labeling reporter dyes to the 5′-terminus and quencher dyes to the 3′-terminus of synthesized ... confirmed to be in the absence of Salmonella spp by means ofthe standard cultural method [1] The DNA of these samples were extracted by three extraction methods and subjected to the multiplex real-time...
... signals were detected, demonstrating the specificity ofthe duplex system Using the same concentrations ofthe primers and probes for EEEV and WEEV, the sensitivity ofthe duplex real-time RT-PCR system ... EEEV compared with the single system In order to improve the sensitivity ofthe duplex system, we further optimized the concentrations ofthe primers and probes for EEEV First, the assay was conducted ... http://www.virologyj.com/content/7/1/284 Page of Figure Analytical sensitivity ofthe duplex real time RT-PCR assay based on the 10-fold dilution series ofthe in vitro-transcribed RNA The amplification blot for EEEV(A) and the associated...
... Moreover, the microcystin-degrading bacteria in the biofilm ofthe biological treatment facility were quantified The bacteria were present in the biofilm throughout the year, suggesting that the biofilm ... in the biofilm in autumn at the practical biological treatment facility, but there is little information about the dynamics ofthe bacteria in lake water and other water bodies Therefore, further ... present in the biofilm ofthe practical biological treatment facility throughout the year, and the abundance ratio ofthe microcystin-degrading bacteria increased to approximately 0.005% ofthe total...
... With these basic tools and an understanding of how to apply them, they can solve most of their control problems themselves What they not need is the theory and mathematics that usually surround the ... thermocouple are known, then the magnitude ofthe e.m.f generated will be a measure ofthe temperature ofthe other junction This other junction is called the hot junction The e.m.f generated for ... that modulates the flow of process fluid through the valve; (b) the seat is the material (metal or soft polymer) that the closure member contacts to shut off the flow ofthe process fluid The control...
... economies of scale as they pool their AP teacher talents with other school districts throughout the state of Wisconsin; • Begin to “level the playing field” of postsecondary preparation for their ... and Mathematics, which were condensed into sixweek, intensive courses Students enrolled in one ofthe four courses, and at the end ofthe coursework, took the appropriate AP exam I took the English ... However, in the summer of 1991 I was fortunate enough to be able to attend a pilot program at the University of Iowa called the Advanced Placement Academy The Academy consisted of AP classes in English,...
... voltage in volts, i is the current in amperes, and R is the resistance in ohms The unit bel, named in honor of Alexander Graham Bell, is defined as the common logarithm ofthe ratio of two power, Px ... gx(t) The signal gain can be expressed in dB as Px gain 10 log10 20 log10 g, Py A:38 since the power is a function ofthe square ofthe voltage (or current) as shown in (A.36) As the ... of an FIR filter can be described as the inner (or dot) product ofthe coefficient and signal vectors, and the result is a scalar y(n) If we further define the coefficient vector a a1 a2 Á...
... A benefit of separating the functionality ofthe Dispatcher consumer, the Run-time Scheduler determines the priority at from the Priority Queues is to allow the implementation of which the event ... supply these types of events (Section 4.2 the correct scheduling priority, relative to the consumers of discusses typed-filtering) Once again, the use of an Event these events Channel makes it possible ... consumer2 show the number of secs the dead- teristics Only the operation’s priority is needed, so the schedlines were missed In other words, consumer0 held the CPU uler can determine how the operation...
... database, there are errors in the use ofthe g r a m m a r code system; for example, Figure illustrates the code for the first sense ofthe noun "promise" I prOmisenl DICTIONARY Once the information ... (central) senses ofthe definition vocabulary together with a classification ofthe relationships between these senses and domain specific concepts, the LDOCE definition of a new word sense often contains ... definition of a word sense in order to produce some representation ofthe sense definition in terms of senses of words in the restricted vocabulary This representation could then be combined, for the...
... intervals Figure shows how the power spectral density (PSD) ofthe oxygen signal changes with time The value ofthe PSD at frequency f is essentially the square ofthe magnitude ofthe corresponding Fourier ... caused the appearance of a cluster of repeated crossings ofthe section In these cases, we averaged the crossing times in a cluster Calculation ofthe cycle time based on the absolute maximum of ... This smoothed signal, which we denote by P40 , was used to normalize the other signals from the mass analyzer in order to correct for long- term drift in the response ofthe instru ment The quantity...
... use of non-standard ports and protocol field values, etc All of these are indicative of different security events and are subject to further correlation Because these tools are also capable of ... all ofthe systems have been patched for the vulnerability or have a non-vulnerable Service Pack level, thus reducing the overall impact ofthe attack and setting an appropriately lower level of ... virtualization spreads in the enterprise One ofthe biggest issues facing security professionals is “virtual sprawl,” or lack of visibility into what is running Given the ease of deployment associated...
... hours The bottomleft shows system statistics, including the total number of tweets, the number of seconds since system start and the average data rate The bottomright table shows trending words of ... occurs The dashboard gives a synthetic overview of volume and sentiment for the candidates, but it is often desirable to view selected tweets and their sentiments The dashboard includes another ... Another crucial feature of this page is that clicking on one ofthe tweets brings up an annotation interface, so the user can provide his/her own assessment ofthe sentiment expressed in the...
... With these basic tools and an understanding of how to apply them, they can solve most of their control problems themselves What they not need is the theory and mathematics that usually surround the ... thermocouple are known, then the magnitude ofthe e.m.f generated will be a measure ofthe temperature ofthe other junction This other junction is called the hot junction The e.m.f generated for ... that modulates the flow of process fluid through the valve; (b) the seat is the material (metal or soft polymer) that the closure member contacts to shut off the flow ofthe process fluid The control...
... In line with these data, the extent ofthe increased expression of these genes was strictly dependent on the number of spiked gp100280–288 specific CTL Most importantly, these results indicate ... also used at the same concentration Moreover, in order to further support the specificity ofthe WB assays, we comparatively evaluated in the same amounts of WB multimer Data from the two donors ... might of decisive relevance in the performance of qRT-PCR and critically affect immune monitoring methods based on the analysis of cytokine gene expression, particularly in the context of field...
... insufficient number of cells Accuracy The accuracy of an analytical method describes the closeness of mean test results (detected) obtained by the method to the true value (expected) ofthe analyte Accuracy ... guide for others in the field to validate these and other similar assays for assessment of patient T cell response These methods can be applied not only to cancer vaccines but to other therapeutic ... under precision -long term inter-assay performance assessment) Precision The precision of an analytical method describes the closeness of agreement (degree of scatter) between a series of measurements...
... designed within the region ofthe COG1F-G1NVR and COG2F-G2NVR PCR products Design was based on a annealing temperature of at least 60°C and a minimum of mismatches between the probe and the other genotypes ... all five ofthe different genotypes of GI stool panel (GI/1, GI/2, GI/3, GI/4 and GI/6) and showed no cross reactivity with any ofthe GII NoV The GII assay detected all Genotypes ofthe Genogroup ... 15 with the genotypes present in the stool panel (Fig 3) The lack of cross reactivity allows these probes to be left on the membrane and as they are designed to anneal to PCR products at the same...