position specific posterior lattices

Ngày tải lên: 31/03/2014, 03:20

... published evidence for the in vivo specificity of bacitracin (or potential products) for PDI inhibition. Our results clearly show that bacitracin in vitro is not a specific inhibitor of PDI, but that ... 0.05 Bacitracin is not specific for PDI A R. Karala and L. W. Ruddock 2456 FEBS Journal 277 (2010) 2454–2462 ª 2010 The Authors Journal compilation ª 2010 FEBS Bacitracin is not a specific inhibitor ... many have been shown to catalyze thiol–disulfide exchange reactions. However, their specific roles, substrate specificities and mechanisms of cooperation with other catalysts and chaperones in the...

Ngày tải lên: 16/02/2014, 14:20

... and  6-fold that of nNOSred (9.0 s )1 ). Calculated values for specificity constants (k cat ⁄ K m ) reveal that CPR has the greatest specificity (10.6 · 10 5 m )1 Æs )1 ) for AqCbl, with MSR (4.1 ... MSR FMN semiquinone (FMN sq ) or FMN hydroquinone (FMN hq ) to the hMS-bound cob(II)alamin [19]. Specifically, the mid- point potential values for FMN ox ⁄ sq and FMN sq ⁄ hq are respectively 380 ... Reactivation of hMS was not observed when MSR was replaced by nNOSred or CPR, highlighting the need for specific protein–protein interactions between MSR and hMS. Reactivation of hMS was found to be dependent...

Ngày tải lên: 18/02/2014, 08:20

... immunoprecipitation (ChIP) assays were performed using SW1353 cells and a specific Sox9 antibody. DNA samples were analyzed by PCR using specific primer pairs generating frag- ments spanning the first (Sox9_1) ... differentiation of the mesenchymal cells to chondrocytes causes a change in ECM composition. Chondrocytes express cartilage- specific type II, type IX and type XI collagen, proteoglycans, aggrecan, and ... embryos were performed using a specific polyclonal antiserum against AP-2e [31] (Fig. 2A). AP-2e was detected in hypertrophic areas of the cartilage. To verify the specificity, we stained sec- tions...

Ngày tải lên: 18/02/2014, 08:20

... fasciculata; CU-NH, pyrimidine -specific ribonucleoside hydrolases; Ec, Escherichia coli; IAG-NH, purine -specific inosine- adenosine-guanosine nucleoside hydrolases; IG-NH, 6-oxo-purine -specific inosine-guanosine ... charac- terization defines SsCU-NH as a pyrimidine -specific NH. This enzyme was completely inactive towards adenosine and guanosine. SsCU-NH, in analogy with Ec-Yeik enzyme, is specific for uridine and cytidine and ... server [62] was used to perform predictions on type and position of secondary structure elements on SsCU-NH, whereas the analysis of type and position of secondary structure elements on the templates...

Ngày tải lên: 18/02/2014, 17:20

... that Lyve-1 expres- sion in liver sinusoidal ECs, reported as a marker of differentiated organ -specific ECs [32] and a potential diagnostic marker of liver cancer and cirrhosis [30], is regulated ... [36]. These results suggest that growth factor signals were transduced properly via endothelium -specific receptors in these cells. Mes- enteric BECs and LECs (Fig. 5A,B) were also obtained by the ... mesenchymal fibroblast cells, even though a conditional genetic modification such as endothelium -specific knockouts can provide a partial solution to this problem. Therefore, the isolation and maintenance...

Ngày tải lên: 18/02/2014, 17:20

... 2000 0 0.05 0.1 0.15 0.2 0.25 0 5000 10 000 0 0.1 0.2 Position of gene in the ranking Density of occurence "high rank" "low rank" ;position Density Genes involved in immune response Fig. ... then analysed regarding its position in the rank. This procedure was repeated 100 times. It turned out that the test group members were strongly present in the top positions of the rank, and this ... 20 000 10 20 30 40 E2F ETS1 HIF-1 HNF4 NFkB Number of genes Number of genes Position in rank Position in rank 0 10 0 10 000 5000 0 10 0 10 0 10 0 10 5 5 5 5 5 Cross-validation...

Ngày tải lên: 18/02/2014, 18:20

... Reed M, Wang P & Tegtmeyer P (1997) Reciprocal interference between the sequence -specific core and nonspecific C-terminal DNA binding domains of p53: implications for regulation. Mol Cell Biol ... target sites possessing a single GG motif at various positions may differ significantly (more details will be published elsewhere). Altered sequence-nonspecific interactions of the p53 protein with DNA ... the nonspecific cisPt-DNA [34]. Furthermore, we were interested in whether the presence of cisplatin adducts within DNA stretches flanking the p53DBSs affects the ability of p53 to bind the specific...

Ngày tải lên: 19/02/2014, 05:20

... stationary growth phase, suggesting that I C is specifically associated with the vacuolar membranes rather than the other cellular membranes. The lipid composition of subcellular membranes in the yeast ... about the content of phosphoinosi- tides in vacuolar membranes and the variation of phospholipid compositions at different growth phases is available. Thus, it remains to be resolved if the tar- geting ... phosphoinositides. The relocation of I C leads us to propose a working model in which I C in the cytoplasm is specifically targeted to anionic phospholipid molecules in the vacuolar mem- branes during the stationary...

Ngày tải lên: 19/02/2014, 05:20

... acid triads for serine protease. Arrowheads indicate the positions and direc- tions of the PCR primers. Primers F1a, F1b, and F1c were specific for exons 1a, 1b, and 1c, respectively. The sequences ... are described in Experimental procedures. (B) Tissue -specific expression of alternative KLK11 transcripts. RT-PCR was performed using primers specific for each alternative transcript, F1a, F1b, or F1c, ... and are expressed in a tissue -specific manner [23]. Human and mouse 5¢ alternative transcripts of KLK6 ⁄ neurosin display identical genomic organization and tissue -specific expression. We propose...

Ngày tải lên: 19/02/2014, 06:20

... volume and a biochemical composition as found in slow-twitch muscle. Also with respect to ATP content in a resting muscle, which is apparently regulated by the muscle-type specific mitochondria, TA ... by 2D high-performance thin layer chromatography (HPTLC) on silica gel HPTLC plates (Merck). The position of cardiolipin on the plates was identi- fied by chromatography of a cardiolipin standard ... c. On the other hand, the up-regulation of F1-ATPase b was clearly smaller and comparable to the composition of SOL mitochondria, indicative of a regulated mech- anism, as discussed below. In addition,...

Ngày tải lên: 19/02/2014, 07:20

... solvent during the nonspecific chitosanolysis by papain and reported lactic acid to be a better solvent owing to the hydrolyzing action of the former [28]. Contrary to this, the specific activity of ... enzyme notably showed a much higher specific activity towards proteolysis of casein (4.14 U) as compared to 1.15 U towards chitosan depolymerization. Although the specificity of pronase was much lower ... on chitosanolysis by pronase Preliminary studies indicated that chitosan could be degra- ded nonspecifically by proteases such as pepsin, papain, protease, etc. It was observed that chitosanolysis...

Ngày tải lên: 19/02/2014, 12:20

... 3¢-noncoding region of the viral RNA is specific and requires the 3¢-poly(A) tail. J. Biol. Chem. 268 , 26093–26098. 36. Banerjee, R. & Da sgupta, A. ( 2001) Specific interaction o f hepa- titis ... than the plus- strand 3¢ UTR. Mapping of the specific NS5B-binding sequence on the 3¢ UTR From the a bove experimental r esults, it i s d erived that a specific element recognized by the viral RNA ... report shows that the CSFV RdRp originating from expression in prokaryotic cells has high template specificity. Replacement and deletion in the middle part of the viral 3¢ UTR has little influence...

Ngày tải lên: 19/02/2014, 16:20

... FACS those cells specifically labeled with destabilized intrabody. The observed stabilization effect upon binding of the short-lived anti-b-gal scFvs may be useful for improving specific imaging ... (Programme ‘Prote ´ omique et Ge ´ nie des Prote ´ ines’) and the Association de la A P. Sibler et al. Specific antigen detection with destabilized intrabody FEBS Journal 272 (2005) 2878–2891 ª 2005 FEBS ... binding-defective 7A-d1-EGFP proteins (Fig. 5G), indicating that the binding of the destabilized scFv allows the specific detection of low amounts of intracellular target. While performing these experiments, we...

Ngày tải lên: 19/02/2014, 18:20

... sub- strates with tail(s), no tails and small linear synthetic substrates as well as direction -specific substrates (see Fig. 5A–J). The sequences and details of these oligo- nucleotides have been ... used in this study are shown in Fig. 5A–J. All the M13 substrates (Fig. 5A–F) including direction specific substrates (Fig. 5I and J) and small synthetic oligonucleotide substrates (Fig. 5G and H) ... kit. 1736 T N. Phan et al. (Eur. J. Biochem. 270) Ó FEBS 2003 A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction Tuan-Nghia...

Ngày tải lên: 20/02/2014, 11:20

... are sugar -specific proteins with multiple combining sites capable of agglutinating cells or precipitating glyco- conjugates [1]. Lectins may recognize specifically the whole sugar [2], a specific ... The lectin from Scylla serrata was NeuGc specific [24] and that from Cancer antennarius was 9-O-NeuAc and 4-O-NeuAc specific [12,28]. Lectins with defined specificity for different kinds of sialic ... O-acetyl NeuAc specificity of P. jacquemontii lectin is sufficiently evident from its inhibition and hemagglutin- atin study. The lectin is unique from that of other sialic acid- specific lectins....

Ngày tải lên: 21/02/2014, 00:20

Ngày tải lên: 21/02/2014, 01:21

Ngày tải lên: 21/02/2014, 15:20

Ngày tải lên: 22/02/2014, 07:20

Ngày tải lên: 06/03/2014, 02:21