pombe mutants with long cls

... each one boil down to, figure out, go through with, come down with, get around to, look forward to, come off, get out of, monkey around with, come up with, go after, put on, doze off, go back on, ... parents died, my grandparents let me live with them What did my grandparents do? 16 Mr Young told some people about the secret information What did Mr Young with the secret information? 17 I saw ... perfect: The tree has fallen over Remember that have can be combined with pronouns as 've: They've never heard of him and has with nouns and pronouns as's: The tree's fallen over He's never heard...

Ngày tải lên: 01/11/2013, 15:20

... 4-ddma-atc and rescreened for induction with 0.4 lm atc and without inducer on MacConkey agar plates We screened 17 600 colonies with mutations in helix a1, 29 840 with mutations in helix a4 and 3900 ... combination of mutations resulting in 4-ddmaatc specificity with those yielding revTetR in the same polypeptide did not yield efficient mutants with combined activities [9] Thus, these properties of ... repression TetRi2-E15A L17V shows repression to 12.5% with 4-ddma-atc and to 58% with atc, thus showing only fivefold improved repression with 4-ddma-atc over atc A similar result is found for...

Ngày tải lên: 20/02/2014, 01:20

... wild-type hMnSOD, with an accompanying 10-fold drop in catalytic activity [23] Interestingly, replacement of Gln143 with a number of different residues, even the conservative replacement with Asn, resulted ... visual inspection Mutants that clearly displayed more rapid growth than Q143A hMnSOD mutants in the paraquat-containing media were selected for further characterization, and other mutants were discarded ... catalysis compared with Q143A hMnSOD: each had kcat ⁄ Km values enhanced by approximately four-fold to fivefold (Table 2) For these mutants, the rate of catalysis was still low compared with the wild-type,...

Ngày tải lên: 07/03/2014, 11:20

... decorin mutants to type VI collagen Type VI collagen was digested with pepsin and immobilized on CM5 chips Surface plasmon resonance measurements were performed with decorin, different decorin mutants, ... applied for h or h at 37 °C After extensive washing with blocking solution, bound proteoglycans were solubilized with 0.1 M NaOH and neutralized with 0.1 M HCl prior to scintillation counting KD ... fibroblasts 293 cells transfected with human wild-type decorin cDNA and human skin fibroblasts were metabolically labelled with [35S]methionine After immunoprecipitation with a monospecific antibody,...

Ngày tải lên: 07/03/2014, 16:20

... we constructed a variety of mutants as well as wild-type enzyme These included mutants with Asp residues in the two active-site DTG motifs changed to Ala residues and mutants lacking the propeptide, ... immunoblotting with polyclonal antibodies to mature rat cathepsin E or rat pro-cathepsin E (Fig 1) The transfected cells gave two intense bands with apparent molecular masses of 46 and 42 kDa with antibodies ... cathepsin E and its mutants T Tsukuba et al Fig Immunofluorescence microscopy of wild-type cathepsin E and its mutants in the transfected HEK-293T cells (A) At 48 h after posttransfection with wild-type...

Ngày tải lên: 16/03/2014, 14:20

... inactive mutant V82A–D25N with patients [8–10] We have reported crystal structures of PR, single mutants in complex with substrate analogs CA-p2 and p2-NC, and double mutants with CA-p2 at resolutions ... interactions of P2¢ with Gly27¢ and Asp29¢ Complex with CA-p2 analog Fig Superposition of the wild type HIV-1 protease (PR), PR with the V82A mutation (PRV82A) and PRV82A in complex with p2-NC.The ... interacted with PR (Fig 5A) Compared with p2-NC, the CA-p2 analog lacked an acetyl group at P4 and cannot form the same van der Waals interactions with PR However, the CA-p2 analog with P2¢ Glu...

Ngày tải lên: 30/03/2014, 20:20

... associated only with higher satisfaction with somatic health and psychological health The patients in the present study were probably more affected by pain than patients with long term pain in ... may not represent patients with long- term pain in general Like life satisfaction, it has repetitively been shown that HRQL is comparatively low among patients with longterm pain, and also that ... domains and correlated the sum to satisfaction with life as a whole, we found that among the patients with chronic pain, satisfaction with family life and with sexual life showed the strongest correlations...

Ngày tải lên: 18/06/2014, 19:20

... × Z2 , and we visualize it as a parallelogram Q in three dimensions with Z3p along the x-axis, Z5q along the y-axis and Z2 along the z-axis, and keep in mind that opposite x and y edges are identified ... true order of magnitude of T (D) than the upper bound Tiling the integers with a long period is equivalent to tiling a long finite cycle in a non-periodic way, as the following easy lemma claims ... that there exists A ⊆ {0, , D} and B ⊆ Z with P(B) = k and A ⊕ B = Z It has been noted by some authors (e.g [2]) that no tilings A ⊕ B = Z are known with P(B) > 2D and A ⊆ {0, , D} So apparently...

Ngày tải lên: 07/08/2014, 07:21

... wi and wj differ.) Two variations of our problem have been studied The first is concerned with finding a longest circuit in Qn having a prespecified minimum run length m, where mrl(n) < m < n This ... additional separation requirement which provides the circuit with error-correcting capability Given d ≤ m < n, one typically seeks a longest circuit W in Qn satisfying (1) and additionally ||i ... circuit in Qa × Qb Remark The (essentially unique) 5-bit Gray code C with mrl(C) = is a special case of the above construction, with a = and b = Here S is the stream S(π1 , π2 , π3 , π4 ) where...

Ngày tải lên: 07/08/2014, 07:21

... patients with RA [4] After an induction scheme with intravenous infliximab infusions given at weeks 0, 2, and 6, infliximab is typically administered at a dosage of mg/kg every weeks in combination with ... attrition of infliximab therapy in patients with longstanding refractory RA over a 4-year period, (b) document the reasons for discontinuation, (c) describe the long- term course of disease activity, ... intensification with infliximab in patients with rheumatoid arthritis Ann Pharmacother 2005, 39:2021 Stern R, Wolfe F: Infliximab dose and clinical status: results of studies in 1642 patients with rheumatoid...

Ngày tải lên: 09/08/2014, 08:22

... patients with a test result outside the reference range No significant differences in laboratory parameters were observed between patients treated with and without Table 3: Number (%) of patients with ... were not significantly different between patient groups treated with or without ABC [8,9] We focussed on HIVinfected patients with long- term use of cART Our findings showed no differences in inflammation ... http://www.aidsrestherapy.com/content/7/1/9 tion markers between HIV-infected patients treated with long- term cART with or without ABC In the current study, we hypothesized that VEGF, an important factor in...

Ngày tải lên: 10/08/2014, 05:21

... of cold blood cardioplegia Mitral valve replacement with a prosthetic valve (Mosaic Ultra Porcine Valve, 27 mm) and tricuspid annuloplasty with a prosthetic ring (Duran Ancore Annuloplasty Band, ... Surgeons, mediastinitis accounted for a quarter of patients with major infections, and the most common clinical predictors associated with mediastinitis were a body mass index of 30 to 40 kg/m2 ... replacement via right thoracotomy approach for prevention of mediastinitis in a female patient with long- term uncontrolled diabetes mellitus: a case report Journal of Cardiothoracic Surgery 2010,...

Ngày tải lên: 10/08/2014, 09:22

... the long- term prognosis of patients with IVL [13,14] Our patient is rare in that he has pulmonary IVL and remains in complete remission more than 24 months after chemotherapy This favorable long- term ... lymphoproliferative disease within small vessels; therefore, strong FDG accumulation in the lower lung field may suggest the presence of many tumor cells spreading hematogenously within pulmonary capillary ... 18-fluorodeoxyglucose positron emission tomography-guided transbronchial lung biopsy in a man with long- term survival: a case report Tomiharu Niida1, Kikuo Isoda1*, Koji Miyazaki1, Soichiro Kanoh2,...

Ngày tải lên: 10/08/2014, 23:21

... associated with increased mortality in necrotizing fasciitis [1-5,8] Patient factors Other factors Age > 60 years Time to operative intervention (surgery delayed >24 hours correlates with relative ... and vein stripping Case Presentation A 39-year-old woman, with no co-morbidity, underwent a left-sided saphenofemoral junction ligation, long saphenous vein stripping and multiple avulsions at ... left groin and thigh with a blister anteriorly Her white cell count was initially normal (8.4 × 109/L) She was in acute renal failure (urea 17.7 mmol/L, creatinine 326 mmol/L) with a metabolic acidosis...

Ngày tải lên: 11/08/2014, 12:20

... adults with longer term mental health problems, the majority of whom have diagnoses such as schizophrenia and schizoaffective disorder Institutions that specifically provide care for people with ... shall also explore the correlation of each item with the total score (item excluded), the average correlation with other items and Cronbach's alpha with that item removed Analysis of associations ... mental illness and optimism for the future with the acknowledgement that pre-morbid functioning may not be fully regained [20] It is corroborated by recent long term outcome studies which have shown...

Ngày tải lên: 11/08/2014, 17:20

... al [3] UK** 2007 Male (with lymphoid infiltration) Epithelioid Surgery Unknown Unknown Survival without relapse Host response Allen RKA [4] Australia** 2007 Female 61 (with inflammatory response) ... (B) The median survival times for patients with an unresectable or postsurgical recurrent MPM are usually reported to be in the 6- to 12-month range with the best supportive care, and even now, ... Survival without relapse Immunological reaction? The present case Japan** 2009 Male (poorly differentiated) Epithelioid Surgery Chemotherapy years months 29 months 58 73 months Survival with re-relapse...

Ngày tải lên: 11/08/2014, 17:21

... lesion depicting persistent, progressive growth, sometimes with calcifications, is compatible with a tumor [2,3,7] Our case was compatible with the socalled odontogenic fibroma complex type (or WHO ... proliferative OE with peripheral rim of columnar cells in a primitive connective tissue stroma without hard tooth formations [23] Variably sized solid nodules of cuboidal OE conspicuously with ductlike ... aggressive and invasive, often treated with limb spearing resections Histologically, DFs are usually far less cellular, with more spindle shaped cells and with intensely collagenous stroma [12]...

Ngày tải lên: 11/08/2014, 20:20

... for viral mutants with a K70E RT mutation The K70E RT mutants were subsequently replaced by K65R RT mutants Similarly to observations with HIV-1 [53,54], when K70E and K65R RTSHIV mutants were ... implications of viral mutants during drug treatment [18] Prolonged tenofovir monotherapy of macaques infected with virulent SIVmac251 resulted in the emergence of mutants with the K65R mutation ... mutants were then rapidly replaced by K65R mutants which in the absence of drug are more replication-impaired in vitro than K70E mutants, but in the presence of tenofovir could outgrow K70E mutants...

Ngày tải lên: 13/08/2014, 09:21

... detected in serum of vaccinated compared with non vaccinated subjects (p>0.05), as well as between vaccinated adults compared with vaccinated children, compared with non vaccinated subjects (p>0.05) ... direct contact with a virus infected individual, or by virus droplets from sneezing or coughing [21] An uncomplicated case of influenza will resolve within 1-2 weeks; however, in patients with other ... correlate with resistance to infection after challenge with wild-type virus [28] Previous studies by others reported that during primary infection, IgG, IgA, and IgM can be detected within 10-14...

Ngày tải lên: 25/10/2012, 11:10

Ngày tải lên: 17/01/2014, 02:20