polymeric micelles for formulation of anti cancer drugs
nanotechnology for cancer therapy, 2007, p.810
... and Robert Langer Section Polymeric Micelles 315 Chapter 17 Polymeric Micelles for Formulation of Anti- Cancer Drugs 317 Helen ... observed in normal tissues by administration of cytotoxic cancer chemotherapeutic agents In addition to the utility of polymeric micelles for delivery of anti- cancer agents,32–35 these nanovectors are ... including polymer conjugates, polymeric nanoparticles, and polymeric micelles Polymeric conjugates that have been investigated for use as nanovector systems for delivery of anti- tumor agents include...
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Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx
... processed even in the absence of exogenous ATP The yield of pTic22 import in the absence of ATP varied between 20–50% of that in the presence of ATP The import of pMGD1 was efficient only at FEBS ... mature forms are indicated by arrow heads A graphical presentation is shown of the influence of thermolysin pretreatment of chloroplasts on the import of pTic22, pMGD1 and pSSU in the presence of ... analyser) of five independently performed experiments for each protein For import, intact chloroplasts were used that were either pretreated or not treated with mg of thermolysin per mg of chlorophyll...
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Báo cáo khóa học: Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide doc
... 2004 for in vitro transcription, in vitro translation and isolation of chloroplasts were as described [19] Expression of GFP-fusion constructs in vivo For transient expression in plant tissues of ... location of targeted proteins in living tissue avoids the potential artefacts of in vitro systems, in particular their lack of specificity Furthermore, the stability of GFP ensures that problems of ... unambiguous means of testing the effect of altering the components of the targeting and import process Acknowledgements We thank Dr Ian Small (INRA, Evry, France) for the GFP plasmids and Professor Colin...
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Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... triggers the insertion of a portion of domain as b-hairpins, which contribute to the formation of a transmembrane pore [34,35] Although domain might play a role for pore formation, our results ... specificity and affinity of toxin fragments for cholesterol We first examined the binding specificity of the toxin fragments to cholesterol by immunostaining with anti- htoxin antibody on lipid-developed ... serum albumin was required for the renaturation of r-d4, resulting in failure in analysis of the toxin structure On the other hand, our procedures for the purification of recombinant domain are...
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drug targeting, strategies, principles, and applications
... Analysis of Antibody Activity The antibody activity of the IT is compared to that of the free antibody and the activity of the IT is therefore expressed as a percentage of the activity of the free antibody ... 4.2 Derivatization of Antibody for Disulfide Linkage Applying more of a more concentrated solution of antibody will eliminate the need for this concentration step, i.e., mL of 10 mg/mL solution ... with the number of mols of antibody, the number of mols of RNase conjugated per mol of antibody can be determined 16 The reaction must be driven to completion by a large excess of RNase, because...
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Nanosized magnetofluorescent fe3o4–curcumin conjugate for multimodal monitoring and drug targeting
... spectrum of pure Cur, IR spectra of OLF–Cur and CSF–Cur showed a significant change (peak form and position) in the range of 3600–3500 cm−1 , which was assigned to the vibration of –OH group of Cur ... 106 cells were incubated with 0.05 mg MNPs for 2–15 h, then treated with either anti- human CD14 antibody (Bio Legend) or actins antibody (Invitrogen) for taking LSCM images 2.2 Fe3 O4 –Cur conjugate ... 2) In the case of CSF–Cur, IR spectra demonstrated the fingerprint band shift of bending vibration of ı(N–H) from 1638 to 1681 cm−1 , indicating binding of iron ions to NH2 group of CS (Fig 3)...
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Báo cáo y học: "Nonimmunoglobulin E–Mediated Immune Reactions to Foods" pptx
... disease and dermatitis herpetiformis, antibodies to TTG and IgA antigliadin and antiendomysial antibodies correlate with expression of the disease However, diagnosis is often confirmed by biopsy ... immediateness of IgE-mediated reactions, the onset of the symptoms of FPIES is delayed from to 10 hours, with a median of hours after the ingestion of food Symptoms typically start with emesis that often ... documentation of typical abnormalities (villous atrophy and cellular infiltrate) that are reversed by the elimination of gliadin from the diet Most patients produce IgA antigliadin and antiendomysial antibodies.7...
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báo cáo khoa học: "Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells" ppt
... (forward), 5’-GAAGATGGTGATGGGATTTC-3’ (reverse) The amplification conditions were as follows: denaturation at 94℃ for 15 min; 40 cycles of 94℃ for 40 s, 56℃ (for Bcl-xL) and 60℃ (for GAPDH) for ... radioresistance of human CRC Introduction Colorectal cancer, one of the most prevalent cancers in the world, is the second most common malignancy and the second leading cause of cancer related ... overexpression of Bcl-xL was correlated with prognosis of colorectal cancer (CRC) patients The aim of this study was to investigate the association of Bcl-xL expression with invasion and radiosensitivity of...
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Báo cáo y học: "CD134 as target for specific drug delivery to T cells in adjuvant arthritis" pps
... measured For analysis of the interaction of CD4+ T cells and liposomes by confocal microscopy, activated A2b cells were incubated with 50 nmol of the different liposomal formulations in medium for ... Subsequently, cells were stained with anti- CD4-FITC, anti- CD134-biotin/streptavidin-PE and anti- TCR-αβ-PerCP, or with anti- CD45RA-FITC, anti- CD134biotin/streptavidin-PE and anti- TCR-αβ-PerCP Cell-associated ... [3H]thymidine incorporation in the presence of antigen or Con A divided by [3H]thymidine incorporation in the absence of antigen or Con A) of triplicate wells For logistic reasons, ILN single-cell...
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Báo cáo y học: "Magnetically retainable microparticles for drug delivery to the joint: efficacy studies in an antigen-induced arthritis model in mice" doc
... of stained particles was observed visually Efficacy of microparticles in antigen-induced arthritis in mice To confirm that all mice were correctly immunised to mBSA, the levels of anti- mBSA antibodies ... All of the groups presented an adequate immune response of the levels of anti- mBSA antibody in the serum compared with an AIA-positive control mouse, with values generally superior to 80% of the ... This extended number of conditions, which led us to the use of a rather limited number of animals per group, Page of 10 (page number not for citation purposes) was necessary for at least two reasons...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
... efforts have been made to improve the anticancer value of cisplatin [14-17] Naturally occurring compounds from diets or medicinal plants are good candidates for increasing cisplatin’s anticancer ... for the treatment of soft tissue sarcoma Clin Cancer Res 2009, 15(10):3472-83 16 Sun Q, Sakaida T, Yue W, Gollin SM, Yu J: Chemosensitization of head and neck cancer cells by PUMA Molecular cancer ... individually or combination of saikosaponin and cisplatin for 30 μM of DHE (A) or μM of CM-H2DCFDA (B) was added 30 before collecting cells The fluorescent intensities of 10,000 cells were analyzed...
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báo cáo khoa học: " The Washington Needle Depot: fitting healthcare to injection drug users rather than injection drug users to healthcare: moving from a syringe exchange to syringe distribution model" pps
... programs For example, the monthly statistics form for needle distribution from the VCH carries an official "performance target" of 90% written at the top of the form The separation of syringe ... first to know, for example, if there is a "bad" batch of drugs on the street, if there is a new hotspot for used syringes, and what the specific needs are for themselves as users and for their peers ... taneously engage in the process of salvaging the same amount of needles The process of exchanging syringes is meant to enforce a kind of personal responsibility for people with addictions This...
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Báo cáo sinh học: " Anti-metastatic effects of viral and non-viral mediated Nk4 delivery to tumours" doc
... vectors have been utilised for Nk4 gene therapy of cancer [9-11], the short lived expression in tumours associated with these vectors may Page of (page number not for citation purposes) Genetic ... generated by cloning of the Nco1, Xba1 fragment of pGL3 (Promega) containing the firefly luciferase gene, by blunt end ligation in the EcoRI, Xba1 region downstream of the CMV promoter of AAV-MCS cloning ... were measured at regular intervals and mice of each group were culled at time points during the trial for analysis of lung metastases The effects of AAV particles on tumour growth are detailed...
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Báo cáo y học: "Custom astrocyte-mediated vasomotor responses to neuronal energy demand" pptx
... loss of glutamate during neurotransmission and provides a potential TCA substrate for synthesis of γ-aminobutyric acid and production of ATP Lactate is also shuttled from astrocytes to neurons for ... PGT-mediated exchange of intracellular lactate for extracellular PGE2 These findings establish lactate as a novel operator of the astrocyte vasomotor switch and are consistent with the ability of lactate ... dominated near the low end of physiological PO2 in brain slices Determination of where the switch point for dilation is may rest with characterizing how low PO2 must fall before ATP production is...
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Advanced control strategies for automatic drug delivery to regulate anesthesia during surgery
... 113 5.8 Performance of MEC controller for 17 patients 114 5.9 Performance of PID controller for 17 patients 115 5.10 IAE for all the 17 patients for set-point change ... Page 6.8 Performance of MPC and PID for sensitive and insensitive patients for the set-point changes during the maintenance period 163 6.9 Average performance of MPC and PID for the set-point ... Schematic representation of the MPC scheme for regulation of BIS 52 3.4 Schematic representation of basic concept of MPC 54 3.5 Setup of a feedback controller for hypnosis regulation...
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Báo cáo sinh học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" potx
... 2007, 4:88 Background Local delivery of genes to vascular wall is a promising approach for the treatment of a number of vascular disorders [1] As a target organ for gene transfer, the vasculature ... investigate the action of regulatory factors of vascular endothelium [17] The cells were transduced at a multiplicity of infection (MOI) of 100 in the presence or absence of mM butyrate, a histone ... percentage of GFP+ cells are indicate above of each bar Cells were transduced with 100 MOI of BacFLT-GFP or BacCMVGFP Data are from four independent experiments ± SD Page of 12 (page number not for...
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Báo cáo hóa học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" docx
... 2007, 4:88 Background Local delivery of genes to vascular wall is a promising approach for the treatment of a number of vascular disorders [1] As a target organ for gene transfer, the vasculature ... investigate the action of regulatory factors of vascular endothelium [17] The cells were transduced at a multiplicity of infection (MOI) of 100 in the presence or absence of mM butyrate, a histone ... percentage of GFP+ cells are indicate above of each bar Cells were transduced with 100 MOI of BacFLT-GFP or BacCMVGFP Data are from four independent experiments ± SD Page of 12 (page number not for...
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Báo cáo y học: "Blood autoantibody and cytokine profiles predict response to anti-tumor necrosis factor therapy in rheumatoid arthriti" pps
... Expansion of RA antigen microarrays for profiling autoantibodies in RA sera To further develop previously-described RA autoantigen microarrays [14], we expanded the number of peptide and protein autoantigens ... development of antigen microarrays and application of these arrays to characterize autoantibody phenotypes associated with a variety of autoimmune diseases [13] We further developed RA antigen microarrays, ... of RA, we reasoned that characterization of spectra of serum autoantibodies and cytokines, rather than characterizing the entire serum proteome, might yield tractable biomarkers for guiding antiTNF...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... value of 0.094 for the CRP(cAMP)2 complex, and no substantial changes in the shape of the emission spectra of the donor have been observed, this justies the lack of the alteration of the Forster ... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... binding of cAMP to the anti- cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85, derived from the crystal structure of CRP(cAMP)2...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... appreciable perturbation of the AMP/ATP ratio Despite our ignorance of the upstream mechanisms, a process for ÔfeedforwardÕ activation of b-oxidation is of physiological interest For example, it could ... dominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling This dominance of the fatty acid effect on AMPK activity provides an explanation for ... means ± S.E.M of 6–12 independent measurements a,b, indicate P < 0.01, < 0.001 for effects of palmitate vs the control (at 60 min); c,d, indicate P < 0.05, P < 0.01 for comparison of 60 with 20...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25