... 3.5 Synthesis of Mannoside Glycan Units of PIMs 3.5.1 Synthesis of Dimannoside of PIMs The planned glycan synthesis commenced with Schmidt glycosylation of the acetimidate donor 30 with the acceptor ... synthesis of a series of mannoside glycans of PIM-1, PIM-2, PIM3, PIM-4, PIM-5 and PIM-6 all with terminally differentiated protecting groups that would facilitate future tagging of desired linkers for ... necessary for LiAlH4 based reductive formation of hindered disubstituted allene from propargylic ethers Chapter concentrated on modified methods for the synthesis of aldehyde intermediates, coupling with...
Ngày tải lên: 10/09/2015, 15:50
... blue G-250 Lane 1, lg of factor X; lane 2, 0.3 lg of factor X; lane 3, lg of factor IX; lane 4, lg of prothrombin; lane 5, lg of protein C; lane 6, lg of protein S; lane 7, lg of Gla-domainless factor ... Briefly, 50 lL of human plasma was incubated with lL of sample at 37 °C for Then, 50 lL of AlexinÒ (purified rabbit brain cephalin) was added and incubated for 1.5 Finally, a 50 lL aliquot of CaCl2 ... method for the permethylation of carbohydrates Carbohydr Res 131, 209–217 40 Yu SY, Wu S & Khoo KH (2006) Distinctive characteristics of MALDI-Q/TOF and TOF/TOF tandem mass spectrometry for sequencing...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... with the calculated monoisotopic [M-2CO2-H]– mass of 1840.42 Da) The mass shift of Da (MR ) M) confirms the formation of the two disulfide bridges However, comparison of the retention times of ... total of 256 summed scans were collected with a relaxation delay of 1.3 s All 1H and 1H-13C correlation assignments were performed using FELIX 2000, which is part of the INSIGHT suite of programs ... treated with 900 lL of trifluoracetic acid in the presence of 50 lL thioanisole, 25 lL EDT and 25 lL DI water at room temperature for 1.5 h After precipitation and washing of the cleaved peptide with...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
... Taiwan, for the use of the MALDI-QTOF instrument This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, CREST of the ... separated into two fractions, glycan B1 and glycan B2, with a molar ratio of 17 : (data not shown) Each of the two minor fractions with GU(ODS) of 8.8 and 11.3 gave a single peak on the amide-silica ... respectively The GU(ODS) and GU(amide) of these four glycans are summarized in Table On the basis of the GU data of glycan A, i.e GU(ODS) of 8.8 and GU(amide) of 5.6, the reference compound 010.1F,...
Ngày tải lên: 23/03/2014, 17:22
Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
... of hexose to the C4 of aldohexonic acid is postulated The proposed structure for HX is Gal(b1–4)GluconA (gluconic acid), which may be interpreted as the C1-oxidized form of lactose A glycan with ... (Fig 2, s4) Glycans with reducing-end hexoses In addition to the N-glycan-derived signals, the LC-MS ⁄ MS data provided evidence for the presence of a group of oligosaccharides of composition H0–3N0–1S0–2 ... truncated versions thereof for most of these oligosaccharides, decorated with up to two Neu5Ac Di-sialyl motifs (Neu5Ac linked to Neu5Ac) were also observed Structural characterization of these oligosaccharides...
Ngày tải lên: 29/03/2014, 09:20
Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... galectins [24]; (b) tested as ligand for surface receptors of different types of tumor cells; and (c) injected into circulation with monitoring of the time course of biodistribution The data set on ... SD) are given for the complete set of analytical data with at least four different experimental series for each sugar receptor The extent of total binding (s) was reduced by that of binding which ... types of sugar receptor in this assay system In contrast, the consideration of the data for the N-glycan with bisecting GlcNAc (B) with reductions in affinity underscores the sensitivity of this...
Ngày tải lên: 30/03/2014, 16:20
New Insight into IELTS Student book with answers 2008 Answers - Part 5 out of 5.pdf
Ngày tải lên: 07/08/2012, 11:48
History Of Modern Biotechnology II
... similarity with the following issue Table Transport properties of various forms of moulds Form of element Transport to element within broth Transport within element Mechanical strength of element ... forms of Acremonium chrysogenum The model consists of balances among these morphological forms, and a number of kinetic equations for growth and product formation and for substrate consumption with ... distribution of the substrate is essential for high process performance Aerobic microorganisms are often used for production; they have to be supplied with oxygen as well Therefore, the fluid dynamics of...
Ngày tải lên: 08/08/2012, 09:49
History of Modern Biotechnology I - Springer
... ethanol Later, Chaim Weizmann of the UK applied the butyric acid bacteria, used for centuries for the retting of flax and hemp, for production of acetone and butanol His use of Clostridium during World ... not compete with chemical means for solvent production, it did provide a base of experience for the development of large scale cultivation of fungi for production of citric acid after the First ... number of British chemists, but the instability of the substance frustrated their efforts Eventually, a study began in 1939 at the Sir William Dunn School of Pathology of the University of Oxford...
Ngày tải lên: 08/08/2012, 09:52
09 Physical and Chemical Characteristics of DDGS revisions.
... (lightness of color) and b* (yellowness of color), with an increase in a* (redness of color) Similar results were also reported by Ganesan et al (2005) Table The Effect of the Rate of Solubles ... they evaluated color in batches of DDGS where approximately 0, 30, 60 and 100% of the maximum possible of syrup was added to the mash before drying Actual rates of solubles addition to the mash ... increasing amount of surface area of an ingredient that is exposed and available for digestive enzymes to act upon Mixing efficiency – a more uniform particle size in a mixture of ingredients will...
Ngày tải lên: 08/08/2012, 10:03
Use of DDGS in Swine Diets
... kg a, b Means within row with unlike superscripts are different (P < 05) Means within row with unlike superscripts are different (P < 10) c, d At the end of the feeding portion of this study, ... 1997; Pork Industry Handbook, 1998) As a result of limited information of feeding DDGS to sows, current recommendations for DDGS inclusion for use of DDGS in sow diets are somewhat different The ... basis as a source of supplemental amino acids at levels up to 44.2% of the diet for gestating sows Monegue and Cromwell (1995) compared reproductive performance of sows fed a fortified corn-soybean...
Ngày tải lên: 08/08/2012, 10:03