pm scl 75a pm scl 75c and pm scl 100 reactivity in elisa
Báo cáo y học: "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity" doc
Ngày tải lên :
09/08/2014, 06:23
... Guth and colleagues [12] showed that arginine-to-serine mutations in VHCDR3 of SN5-18 ablate binding to chromatin The single arginine-to-serine mutation in B3(R27aS)VL did not ablate binding to ... to bind dsDNA In particular, reversion of R27a to serine (R27aS) in B3Vλ CDR1 resulted in a significant reduction in dsDNA binding, indicating the importance of this arginine at the binding site ... suggests that binding is stabilised by interaction of dsDNA with three arginines in B3, one each in the complementarity-determining region (CDR1) and CDR2 of the light chain and another in CDR2 of...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... lymph node proteins revealed a radioactive band of 25 kDa in liver and bands of 30 and 56 kDa in thoracic lymph nodes (Fig 5C) No radioactive protein bands could be detected in spleen samples ... deposited in the airways of mice and metabolized The labelling method involves the incorporation of a selenocysteine residue and the gamma-emitter selenium-75 (75Se) within an engineered C-terminal...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... are no clashes of amino acid side chains in the core of the structure, and most mutations occur on the protein surface The FAD binding groove is conserved with most ligand binding residues identical ... misfolding of the short Erv1p because FAD binding and in vitro enzyme activity are unchanged Changing C30 or C33 to serine drastically increases the amount of observed dimers Probably, the remaining ... The C-terminal FAD binding domain of Erv1p and Erv2p are conserved Partial structural information for the homologous Erv2p is available in the databases [11] The crystallized protein is a 119...
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Báo cáo hóa học: "Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma" potx
Ngày tải lên :
18/06/2014, 16:20
... matured with TNF-a, IL-1b, IL-6 and prostaglandin E2 (PgE2), pulsed with a mixture of 20 MHC class I and II-binding peptides and administered subcutaneously Disappointingly, the DC arm had a response ... fixed and permeabilized with the Intrastain kit, and stained for intracellular IFN-a with an anti-IFN-g-APC antibody Cells were resuspended and stored for up to days in PBS 1% Cytofix before being ... cryopreserved in liquid nitrogen The cryopreserved product was transferred to the clinical site with continuous temperature monitoring The vaccine was administered within one hour of thawing and reconstituting...
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Báo cáo hóa học: " Are quantum dots ready for in vivo imaging in human subjects?" docx
Ngày tải lên :
22/06/2014, 18:20
... use in clinical applications such as in vivo imaging in human subjects Modeling studies have revealed that two spectral windows exist for QD imaging in living subjects, one at 700–900 nm and ... maintains the native ligands on the QDs and uses variants of amphiphilic diblock and triblock copolymers and phospholipids to tightly interleave the alkylphosphine ligands through hydrophobic interactions ... antagonist) containing peptides were conjugated to QD705 (emission maximum at 705 nm) and QD705-RGD exhibited high affinity integrin avb3 specific binding in cell culture and ex vivo In vivo NIR fluorescence...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
Ngày tải lên :
11/08/2014, 00:22
... Biosciences), which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation ... biodistribution is independent of the routes of administration, such as intravenous injection [13-15,18,53,75,78,79], intraperitoneal injection [26], intratracheally instillation [77], and inhalation ... Nanjing, Nanjing University (Nanjing, China) The streptavidinIRDye 800CW was purchased from Li-Cor Biosciences (Lincoln, NE, USA) The main chemicals, including EDC, HEPES, glutaraldehyde and...
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báo cáo khoa học: "BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer" ppsx
Ngày tải lên :
11/08/2014, 00:23
... cancer of mm in diameter and IVIS imaging system and Magnetic Resonance Imaging, investigated the feasibility of as-prepared nanoprobes for non-invasive in vivo targeted dual modal imaging of gastric ... polarization rate of the surrounding molecules, and resulting in the decrease of stokes displacement, finally resulting in a blue shift in the emission spectra Similarly, magnetic intensity of as-prepared ... modal imaging such as fluorescent imaging and magnetic resonance imaging, and own an obviously specific targeting ability toward a gastric cancer tissues with mm in diameter during 0.5 h and 12...
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Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa
Ngày tải lên :
13/08/2014, 09:21
... coding regions (pSVR∆GP-CMVGFP) and also the generation of a self inactivating (SIN) vector (pSVR∆SIN-CMVGFP) created by additional deletion in the 3' UTR This will be copied into the 5'LTR during ... packaging relationship between SIV and HIV-2 Interestingly, the major packaging determinant of both HIV-2 and SIV has been shown to be upstream of the major splice donor [9,10] and by inference ... encompassing gag and pol sequences) was discarded The remaining fragment was religated and formed pSVR∆GP CMVGFP was obtained from SalI digestion of pSVR∆-CMVGFP and ligated into the SalI linker...
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Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc
Ngày tải lên :
13/08/2014, 13:20
... detected an increasing NAD(P)H autofluorescence representing an intracellular inadequate oxygen supply Both changes are accompanied by increasing markers of liver cell injury Future therapeutic interventions ... participated in animal procedures and in drafting the paper All authors approved and read the final manuscript Acknowledgments This work was supported by the Interdisziplinäre Zenrum für klinische ... towards a reduction in liver oxygen supply 6.0 h I/R, mice were further randomized to a group treated with a continuous infusion of ET-1 (70 pmol/min., i.v.) starting 15 minutes prior to microscopy...
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Báo cáo y học: "Human T-cell leukemia virus type 2 post-transcriptional control protein p28 is required for viral infectivity and persistence in vivo" ppt
Ngày tải lên :
13/08/2014, 05:20
... needed to understand the function of p28 Such studies will entail identifying the functional domains of the protein involved in localization, protein interactions, and RNA binding as well as precisely ... which introduced a stop codon at amino acid of the p28 ORF and had no affect on the overlapping Tax and Rex amino acid sequence We initially determined whether knocking out p28 altered Tax and/ or ... transfectants containing the desired proviral clone were isolated following incubation in 24-well culture dishes in medium containing mg/ml Geneticin (Gibco, Carlsbad, CA) Following a 4–5 weeks...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
Ngày tải lên :
08/08/2014, 17:20
... TFF1 and WISP2 Furthermore, the list contained several genes involved in the estrogen response including Phase I estrogen metabolizing enzymes (CYP1A1 and CYP1B) and Cyclin D1, a competitive inhibitor ... through 1) up-regulating proteins involved in estrogen metabolism (specifically through increasing the CYP1A1/1B1 ratio), and 2) decreasing BRCA1 inhibition thus permitting its inhibition of estrogen ... were grown in RPMI 1640 supplemented with 10% fetal bovine serum, penicillin, and streptomycin (Gemini Bio-Products, West Sacramento, CA) and incubated at 37˚C with 5% CO2 Iodine and estrogen...
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Báo cáo y học: ""Shock and kill" effects of class I-selective histone deacetylase inhibitors in combination with the glutathione synthesis inhibitor buthionine sulfoximine in cell line models for HIV-1 quiescence" doc
Ngày tải lên :
12/08/2014, 23:21
... replication in U1 and ACH-2 cells, and toxicity in uninfected JurPotencies Potencies of different HDACIs in terms of activation of HIV-1 replication in U1 and ACH-2 cells, and toxicity in uninfected ... HDACIs with increased selectivity and decreased toxicity Finally, we are currently searching for novel γ-glutamyl-cysteine synthetase inhibitors acting in the nanomolar range and displaying lower ... far from being fully understood [26] In general, oxidative stress tilts the balance of HAT/HDAC activity towards increased HAT activity and DNA unwinding, thus facilitating the binding of several...
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INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
Ngày tải lên :
02/10/2015, 17:15
... Peroxidase xiv IL-1 Interleukin-1 IL-1β Interleukin-1β IL-6 Interleukin-6 IL-8 Interleukin-8 IL-10 Interleukin-10 IL-18 Interleukin-18 i.t Intrathecal L-PGDS Lipocalin-type Prostaglandin D Synthase ... are involved in the early development of chronic pain, while astrocytes function in sustaining the pain (Vallejo et al., 2010) An interesting finding was that nerve injury induces an increase in ... neuropathic pain 1.2.3 Pro-inflammatory Cytokines The role of pro-inflammatory cytokines, namely tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), in the initiation and...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
Ngày tải lên :
02/11/2012, 11:17
... persistently HBV expressing cell line HepG2.215 and its parent cell line, a non-HBV expressing cell line HepG2 We found that cIAP1 and cIAP2 were clearly increased in the HBV expressing cells but XIAP ... carcinoma cells, lung cancer cells [7, 10 ], prostate cancer cells [24] and renal carcinoma cell line [28, 19, 44 ] Other interesting finding is that the cancer cells resistant to the radio- and ... and procaspase-7 by binding and blocking the activity of caspase-9 Increasing evidence demonstrated that IAPs are up regulated in many human tumor types and tumor cell lines [ 18, 32, 29, 39,...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins ... without and with iron Thin lines represent background staining with mouse Igs (B) Histograms show background staining with no antibody (thin lines) or with rabbit Igs (thick lines) as first step in ... [14] Interestingly, in this study, iron-treated cells showed a marked decrease in Apo B100; a protein essential for maintaining normal lipid metabolism Hepatic iron overload has been reported in...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... sequence, increasing the number of basic residues of lysine, arginine and histidine, could occur However it seemed probable that the eEF1A isoforms could act as a controlling event in maintaining the ... nuclear and cytoplasmic extracts were blotted and assayed for the nuclear proteins Egr1 and TBP The results displayed in Fig 1B clearly indicate that the proteins Egr1 and TBP were detected only in ... were incubated in buffer containing 25% glycerol, with c32P-labelled GT probe in the presence of lg poly(dIdC) and lg of CT as competitors, as described in the Materials and methods After 25 of incubation...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
Ngày tải lên :
21/02/2014, 00:20
... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... alteration in PKB protein was similar to that in PKB activity (Fig 7), suggesting that the insulininduced PKB protein was almost fully phosphorylated and activated Whether insulin was internalized...
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Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology pdf
Ngày tải lên :
06/03/2014, 09:22
... enzyme of interest should be investigated in the context of the in vivo-like medium Finally, in vivo, the enzymes are present at much higher concentrations than in typical enzyme assays, in which ... AB, Van den Brink J, Lindenbergh PA, Teixeira de Mattos MJ, Smits GJ, Daran-Lapujade PAL et al (2006) Standardization and In vivo’-like enzyme activity measurements in yeast In 2nd International ... has been used extensively to characterize fermentation in the CEN.PK113-7D strain [8,48,49] and it was the starting point for standardization in the Vertical Genomics Consortium [50,51] The high-phosphate...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
Ngày tải lên :
07/03/2014, 00:20
... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... validation of the Swi1 and Snf5 activator-binding domains is also of interest in relation to the twostep binding mechanism between Gal4 and Swi1 that has been characterized in vitro [34], and our results ... GAL2 and GAL7 regulatory regions in strains lacking either or both the Swi1 (residues 329– 655) and Snf5 (residues 2–327) activator-binding domains, 30 after galactose induction Quantifying recruitment...
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Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx
Ngày tải lên :
07/03/2014, 00:20
... injected in the gonad with 100 lL of saline solution containing dsRNA, or saline solution for the control After dsRNA injection, oysters were maintained in raceways in conditions allowing optimal ... Effects of in vivo oyvl-dsRNA injection on germ cell development in oysters, month postinjection (A) Female control, injected with saline solution Oocytes are in vitellogenesis (B) Female injected ... protein extract from each tissue was loaded into the gel A single band of about 79 kDa was detected in female and male gonads Fig In vivo dispersion of DIG-labelled oyvl-dsRNA injected into oyster...
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