plant growth and development

... homeostasis and normal growth and development (Hanfrey et al., 2002) SAMDC expression varies spatially and temporally during plant growth and development SAMDC expressed strongly in actively dividing and ... embryogenesis of eggplant (Sharma and Rajam, 1995; Yadav and Rajam, 1998), carrot (Noh and Minocha, 1994) and rice (Bajaj and Rajam, 1995) and rhizogenesis of tobacco (Altamura, 1994) and poplar (Hausman ... 50 2.5.2 Plant defense to herbivory 52 2.5.3 Plant growth and development 53 Interaction between PAs, ethylene and GABA 56 Materials and Methods 59 Plant materials 59 3.1.1 Mustard 59 3.1.2 Arabidopsis...

Ngày tải lên: 12/09/2015, 10:19

... Restriction endonucleases generate ends that facilitate mixing and matching GAATTC CTTAAG GAATTC CTTAAG EcoRI cut G AATTC CTTAA G G AATTC CTTAA G Mix and ligate G AATTC CTTAA G Recombinant molecules G AATTC ... into bacteria • Lambda phage and P1 phage can carry large fragments of DNA – 20 kb for lambda – 70 to 300 kb for P1 • M13 phage vectors can be used to generate single-stranded DNA YAC vectors for ... Denature, anneal primers, and synthesize new DNA: duplex molecules of desired product PCR, cycle 5: exponential increase in product Cycle 5: Denature, anneal primers, and synthesize new DNA: 14...

Ngày tải lên: 13/03/2014, 18:39

... higher plants Plant Physiol Biochem 2004, 42(4):265-271 May MJ, Vernoux T, Leaver C, Montagu M, Inze D: Glutathione homeostasis in plants: implications for environmental sensing and plant development ... 44(4):481-512 Oguntunde P: Whole -plant water use and canopy conductance of cassava under limited available soil water and varying evaporative demand Plant and Soil 2005, 278:371-383 El-Sharkawy MA, ... plants: Molecular and genetic perspectives Physiol Plant 2001, 112(2):152-166 Belknap WR, Garbarino JE: The role of ubiquitin in plant senescence and stress responses Trends plant sci 1996, 1(10):331-335...

Ngày tải lên: 12/08/2014, 05:20

... polymerase (Syntol, Moscow, RF); and 5pmol of both forward and reverse primers and 3,5 pmol probe The real-time PCR run was 10 at 95°C, and 50 repeats of 20 sec at 95°C and 50 sec at 62°C All samples ... kit, protein A and phenol-chloroform extraction method – molecular size standards; 2, and 10 – PCR-positive serum from patient M; 3, and 11 – PCR-positive serum from patient P; 4, and 12 – PCR ... (chlamydial 16 S rRNA and omp1) using DNA extracted from human plasma by phenol-chloroform method – molecular size standards; 2, and – PCR positive serum from patient M.; and – PCR positive serum...

Ngày tải lên: 26/10/2012, 08:57

... template and then sequenced Finally, two cDNAs were obtained and designated HRT1 and HRT2, respectively DNA sequencing analysis Materials and methods Plant materials and RNA isolation Latex and various ... AB061234 and AB064661 respectively The ORFs of HRT1 and HRT2 encode 290 and 284 amino acid residues (87.3% identity) with predicted molecular masses of 33.2 and 32.8 kDa respectively Hydropathy and ... 50 °C and at 72 °C with a preheat at 95 °C and a 10 final extension at 72 °C using primers, S1 (5¢-GCAAATGCAACTGGA AGCGG-3¢) and A1 (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) for amplification of HRT1, and primers...

Ngày tải lên: 07/03/2014, 21:20

... visible and expression increases steadily in the regenerating tissue after 13, and 18 h, (C) and (D), respectively After 24 h, the ppod1 expressing area is not further expanding (E), and after ... mucous cells (fm), and granules without diaminobenzidine-staining as a control (D) Scale bars are mm in (A), 20 lm in (B), and 1.5 lm in (C) and (D) considered to be aged cells, and they contain ... diaminobenzidine and H2O2 As can be seen in Fig 5A, a major and a minor activity exist in the animal Only the major activity (band I) is present in the feet of the animals Therefore, band I was regarded...

Ngày tải lên: 08/03/2014, 10:20

... L19, L22, and human mrps MRP-L43 and MRP-L49, respectively The gene for each protein was cloned into the plasmid pSHLeu to attach an S-peptide tag as described in Materials and methods and the ... (Dydr116c), Dmrpl50(Dynr022c) and Dmrpl51 (Dypr100w) were streaked on YPD and YPGE plates and incubated at 30 °C B, Strains with S-tagged MRPS8 (Dmrps8/pSHLeu-MRPS8) and with HSV-tagged MRPS8 (Dmrps8::MRPS8HSV) ... harboring Dmrps8 and Dmrps16 deletions are a-type haploid derivatives of BY4743 Strains with Dmrps35, Dmrpl1 and Dmrpl51 deletions are derived from BY4741, and those with Dmrpl50 and the HSV-tagged...

Ngày tải lên: 17/03/2014, 10:20

... R44 and K45, and K45 and W46 adjacent to the proposed catalytic centre and biotinyl-5¢-AMP binding site Chemical crosslinking of BPL and BCCP Although structures of E coli BirA and both apo- and ... aeolicus The A aeolicus bpl and bccpD67 genes were amplified from A aeolicus genomic DNA template by polymerase chain reaction using primers BPL-for and BPL-rev; and BCCPD67 and BCCP-rev, respectively ... supplemented with kanamycin (30 lgÆmL)1) and grown overnight at 37 °C and 250 r.p.m This seed culture was then used to inoculate L of fresh growth medium and grown at 37 °C to D600 ¼ 1.0 before...

Ngày tải lên: 31/03/2014, 07:20

... heme, tetrahydrobiopterin and the substrate arginine, and (b) the electron-supplying reductase domain that binds NADPH, FAD and FMN Communication between the oxygenase and reductase domains is ... determination, and activities of two bioactive peptides from the skin glands of the stony creek frog Litoria lesueuri We have not studied a member of the L lesueuri complex of frogs previously, and we ... a Millex HV ®lter unit (0.45 lm), and lyophilized This work conforms with the Code of Practice for the Care and Use of Animals for Scienti®c Purposes (1990) and the Prevention of Cruelty to Animals...

Ngày tải lên: 31/03/2014, 15:20

... at the intake and discharge of all fans and air-handling equipment to reduce vibration transmission 10 air Inertia bases (type C) are recommended for all class and fans and air-handling equipment ... at the intake and discharge of all fans and air-handling equipment to reduce vibration transmission 10 air Inertia bases (type C) are recommended for all class and fans and air-handling equipment ... at the intake and discharge of all fans and air-handling equipment to reduce vibration transmission 10 air Inertia bases (type C) are recommended for all class and fans and air-handling equipment...

Ngày tải lên: 31/03/2014, 18:20

... first-strand cDNA is then G-tailed by terminal transferase and second-strand cDNA is synthesized using an oligo(dC) primer in a PCR reaction The firststrand cDNA on beads can be isolated and reused ... position A variety of internal standards can be used Routinely, we use probes for GAPDH and β-actin, Rb, and p16 or p21 to identify the housekeeping, abundant, and rare species of mRNAs, respectively ... interests and determine whether these genes are differentially expressed Altered gene expression of certain molecules and their related receptors may shed light on the developmental, physiological, and...

Ngày tải lên: 11/04/2014, 01:04

... tables and stand assortment tables, models of quality and models of damage to stands Models of yield tables (Petráš, Mecko 2001, 2005) determine the development of mean diameter dv and standing ... 35 -100 Yield (thousands SKK) Yield in thousands Yield in thousands SKKSKK 00 500 500 Yield in thousands SKK 00 Yield (thousands SKK Yield in thousands SKK) Yield in thousands SKK 00 600 600 ... the gross and net yield of the main3stand in 11 Fig I-214 – the 27 29 31 33Age (years) of the main stand in gross and net yield Age (years) site index and age Age (years) site index and age dependence...

Ngày tải lên: 07/08/2014, 03:22

... larici-populina occurrence and the value of growth traits and biomass yield in Populus nigra The correlation between the growth traits of stem diameter and plant height and the intensity of rust ... times four plants The growth traits of stem diameter at a height of 0.5 m to the nearest mm and plant height to the nearest 0.1 m were measured at the end of the growing season In 2007, the growth ... photosynthetic efficiency, premature defoliation and increased susceptibility to other pests and diseases On the widely planted cultivar ‘Beaupré’, growth restriction due to rust was estimated to...

Ngày tải lên: 07/08/2014, 10:21

... according to their growth and quality Poplar clones I-214 represent a group of clones with strong diameter growth and lower quality stem including the clones Blanc du Poitou, Pannonia and Gigant On ... was calculated for each clone and its quality class I–VI of logs, which characterizes their systematic component, and standard deviation that characterizes their random component, it means model ... with zero and 40% damage of stems of quality class A The structure of the assortments for the stands with average quality of stems of class B is illustrated in Figs and In these stands the proportion...

Ngày tải lên: 07/08/2014, 10:21

... Results Viru s is olatio n Two ALVs, KOAL-PET and KOAL-HD, were isolated from broiler breeder flocks One of the viruses was isolated from grandparent, and the other was from parent stocks (Table ... Science and Engineering Foundation References Arshad, S S., K How es, G S Barron, L M Smith, P H Russel, and L N Payne Tissue tropism of the HPRS-103 strain of J subgroup avian leukosis virus and ... 181-187 Bai, J , L N Payne, and M A Skinner HPRS-103 (exogenous avian leukosi virus, subgroup J ) has an env gene related to those of endogenous elements EAV-0 and E51 and an E element found previously...

Ngày tải lên: 07/08/2014, 15:20

... Streptococcus and Lactobacillus) and pH 3.0 (for Bifidobacterium), respectively, by using N HCl, and put into ml per a ml vial Streptococcus and Lactobacillus were inoculated into MRS broth (pH 2.5) and ... media and were transferred onto BL media They were incubated at 37oC for 24 hrs under anaerobic conditions, and regarded LAB as Streptococcus, Lactobacillus and Bifidobacterium by aerobic growth, ... probiotic use feces were serially diluted with the Diluent A, and spreadplated as 10−1, −3, −5, −7 onto TATAC, LBS and BS media and as 10−5, −6, −7 onto BL media [15,21] Plates were incubated...

Ngày tải lên: 07/08/2014, 18:20

... on solid Gresshoff and Doy (GD) medium (1972) and woody plant medium (WPM) (Lloyd and McCown, 1980), respectively, supplemented with 0.5 mg/l benzylamino-purine (BAP) The explants were kept in ... difficult In Quercus robur and Quercus petraea, rooted plantlets (Vieitez et al, 1985; Evers et al, 1987; San José et al, 1988, 1990; Juncker and Favre, 1989) as well as plants in soil (Meier-Dinkel, ... Selection and Improvement of Forest Reproductive Material Bologna, 2-3 June 1987, vol 2, 62-70 Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato) Planta...

Ngày tải lên: 08/08/2014, 19:21

... rice (Gomez and De Datta, 1971),wheat (Konovalov and Loshakova, 1980), Norway spruce (Gaertner, 1983) and poplar (Hansen, 1981; Zavitkovski, 1981; Bisoffi, 1988) Moreover, Cannell and Smith (1980) ... which there is no further tree height growth gradient" When drip irrigation and fertilization were sufficiently supplied both on the plot and far beyond the unplanted alley, only canopy competition ... for light can be responsible for the development of a border width and a homogeneous plot center In our case irrigation water and fertilizers were sufficiently and uniformly supplied but only on...

Ngày tải lên: 08/08/2014, 19:21

... branch length and diameter, and the angles of origin and termination were determined for both proleptic and first-order sylleptic branches All branches on a given year’s height growth increment ... on other growth determinants (canopy density, rates of photosynthesis and respiration, patterns of translocation and growth analysis) have meanwhile been collected for the same clones and will ... increment (HGI) were counted and measured Dry weight (DW) of proleptic and sylleptic branches, current terminal and the remainder of the stem were determined, as well as DW and leaf area (LA) of leaves...

Ngày tải lên: 09/08/2014, 02:21

... BTA27 and BTA29, and could be used as a standard for cytogeneticists using different banding techniques (G, R and Q) Each BAC has just been recently localised by FISH on R-banded and G-banded ... G-banded metaphase chromosomes: this GTG standard karyotype created the basis for all subsequent nomenclature efforts [5] With the development of prometaphase chromosome preparations and R-banding ... BLAST program and submitted to GenBank 2.4 Chromosomal assignment using the INRA hamster-bovine somatic cell hybrid panel The panel was constructed by Heuertz and Hors-Cayla [9] and is composed...

Ngày tải lên: 09/08/2014, 18:21