pi3k akt signalling pathways and its modulation by the a3 receptor
Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx
Ngày tải lên :
07/03/2014, 17:20
... procedures) were separated by centrifugation and analysed, in parallel, by 1H NMR spectroscopy and by TLC Consumption of the substrates and production of the final metabolites (succinate and acetate) were ... protein) were then further incubated with their substrate for h Samples of the incubation were taken at 1, and h, and extracellular and intracellular media were separated by centrifugation and analysed ... nm and by succinate and acetate assays from 1H NMR spectra, respectively Maltodextrins of DP > were clearly metabolized by the cells, as shown by the following that (i) their presence allowed the...
- 12
- 406
- 0
Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
Ngày tải lên :
24/03/2014, 04:21
... ngÆlL)1 and 3.0 pgÆlL)1 The concentrations of the experimental samples were calculated by comparison with the standard curves Background fluorescence was removed by setting a noise band The number ... the figures The times of exposure were the same for Fig 2A and B The reproducibility of the Western blot quantification was analyzed using mouse gastrocnemius and tibialis muscle mitochondria The ... access to water The animals were killed by cervical dislocation, and tibialis anterior, gastrocnemius and soleus muscles were carefully dissected and kept on ice In fasted animals and the respective...
- 7
- 535
- 0
Báo cáo y học: "The gene expression profile of preclinical autoimmune arthritis and its modulation by a tolerogenic disease-protective antigenic challeng" ppt
Ngày tải lên :
12/08/2014, 18:20
... [53] Furthermore, CXCR3 and its ligands are involved in the selective recruitment of Th1 effector cells into the sites of tissue inflammation [54, 55] The gene for the receptor for another chemokine, ... manuscript and helped in designing the experiments CL and MTT performed data analysis and helped in writing a part of the Methods section KDM designed the study, interpreted the data and wrote the manuscript ... influenced by Bhsp65 These genes showing altered expression encoded the leukocyte-specific markers and receptors, cytokines/receptors, chemokines/ receptors, adhesion molecules, components of the complement...
- 48
- 317
- 0
Báo cáo khoa học: Binding of the viral immunogenic octapeptide VSV8 to native glucose-regulated protein Grp94 (gp96) and its inhibition by the physiological ligands ATP and Ca2+ Ming Ying and Torgeir Flatmark pot
Ngày tải lên :
30/03/2014, 11:20
... Mr,analyte and Mr,ligand are the relative molecular mass of the analyte and ligand, respectively; n is the number of analyte-binding sites on the protein; (A) is the fraction of active sites on the ... signal (Fig 2C), and the response isotherm gave a [A]0.5 of 168 lm Although Grp78 ⁄ BiP and Grp94 are the two major recipients of the pool of ATP translocated into the lumen of ER [24] the effects ... that Grp94 and six other ER proteins were selectively bound to an affinity column of denatured histone and specifically eluted by ATP, and that the release of Grp94 was further stimulated by Ca2+...
- 10
- 304
- 0
Báo cáo y học: " Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein" ppsx
Ngày tải lên :
12/08/2014, 23:22
... first PCR, the exon1 sense strand partially hybridizes with the exon2 antisense strand and the exon1 antisense strand with the exon2 sense strand Then "Amplification PCR" resulted in the accumulation ... translation of the mono-Glob RNA is cap dependent As expected, the 7m-GTP cap analog reduced by fold the translation of the capped mono-Glob RNA in the RRL (lanes and in top and bottom panels) and had ... translation of the mono-Tat RNAs was not altered by the addition of the 7m-GTP cap analog (Fig 3E, top and bottom panel, lanes 6–9) Taken together, these results show that in the RRL the HIV-1 Tat...
- 18
- 240
- 0
Báo cáo khoa học: PI3K⁄Akt signalling-mediated protein surface expression sensed by 14-3-3 interacting motif pot
Ngày tải lên :
07/03/2014, 00:20
... infection activates the pro-survival PI3K ⁄ Akt pathway and thereby extends the lifespan of infected macrophages [36] Our results suggest that the PI3K signalling pathway may play further roles in ... for wildtype GPR15 The enhancement by these treatments was efficiently blocked by the PI3K inhibitor LY294002, which supports the concept that PI3K activation is necessary for the growth factor-induced ... Kir2.1-RKRSWTY and 14-3-3 (Fig 3C, lanes and 3) The inhibitors for either PI3K (wortmannin) or Akt (API-2), but not MEK inhibitor (PD98059), reduced the induction of the surface expression by fetal...
- 12
- 244
- 0
Báo cáo khoa học: Characterization of testis-specific serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc
Ngày tải lên :
23/03/2014, 11:20
... detergents and only piridinium betain and CHAPS not abolish its activity Taken together these results established the conditions for the in vitro kinase reactions with TSSK3 in further experiments ... S166 may either be part of the T168 recognition motif or may potentially be autophosphorylated and thereby replace the requirement for T168 phosphorylation The kinase activity of these mutants ... activity [33] Therefore, it is thought that the role of PDK1 in the activation of other kinases is governed by its cellular location For example, in insulin-induced activation of PKB ⁄ Akt the insulin-induced...
- 14
- 374
- 0
ESTROGENIC ACTIVITY LEVEL OF NAKDONG RIVER BASIN AND ITS CONTROL BY WATER TREATMENT PROCESSES
Ngày tải lên :
05/09/2013, 08:40
... dichloromethane and distilled water after filtration by a glass fiber filter (Whatman GF/B, previously rinsed with MeOH and dried) The SPE column was eluted by dichloromethane, and then the samples ... ethinylestradiol) Upon binding an active ligand, the occupied receptor then binds to the estrogen-response sequences (EREs) and interacts with transcription factors and other transcriptional components to ... substances and activity is distributed over the whole middle and downstream excluding the upstream of Andong Relative estrogenic activity of estrogenic substances in the Sewage treatment plants and...
- 6
- 484
- 0
Cadmium Removal by the Hydroponic Culture of Giant Reed (Arundo donax) and Its Concentration in the Plant
Ngày tải lên :
05/09/2013, 10:17
... circulated by a liquid pump To avoid the direct effects on the removal of cadmium and phosphorus by parts other than the rhizome, the water level of the cultivation pot was kept lower than the top of the ... to design the multi-functional wetland The purposes of this study are thus to clarify the dynamics of cadmium removal by giant reed, and investigate its concentration in plant body The dynamics ... 25°C The composition of the culture broth shown in Table was basically the same as that in the previous study (Sagehashi et al., 2009) The volume of the total culture broth was set as L and was...
- 7
- 701
- 0
Expansion and its realization in the short story “the law of life” by jack london
Ngày tải lên :
29/01/2014, 14:44
... between the writer and the readers, and the other between the characters (the main character and his son) In this story, the writer plays the role of the narrator using neutral and daily lexis The ... symmetrical And the sentences “Joe watched TV, and Mary washed the clothes” and “Mary washed the clothes and Peter cleaned the floor” imply “Joe watched TV, and Peter cleaned the floor” In parataxis, the ... a Rheme The Theme is the element which serves as the point of departure of the message, and the Rheme is the part in which the Theme is developed According to Eggins (1994: 275) the Theme typically...
- 43
- 921
- 0
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... stefin A and stefin B at pH and variant of stefin B and the P79S mutant of the variant at both pH and pH Each panel is a plot of heat change on ligand addition (kcalÆmole)1) against the ligand ⁄ stefin ... A binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macromolecule ratio From the binding isotherm values for the reaction ... was prepared by collecting the corresponding peak from the Superdex 75 column, then, the monomer was incubated (see above) in the presence of Cu2+ and without Cu2+, at pH and pH The mobile phase...
- 14
- 586
- 0
Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx
Ngày tải lên :
20/02/2014, 01:20
... where kA and k0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k2 and k1 are the rate constants of the inhibition of the bilitranslocase– ... [A] tested; k2 ⁄ k1, the value of the intercept in the Scrutton and Utter plot, where k2 and k1 are the rate constants of the inhibition of either the bilitranslocase-ligand complex or free bilitranslocase, ... electrophoretic mobility exhibited by the carnation petal and the rat liver carriers is the same The antisequence anti-bilitranslocase Igs The antibodies (A and B) used to obtain the above summarized results...
- 15
- 589
- 0
Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx
Ngày tải lên :
08/03/2014, 08:20
... mgÆmL)1), and dashed lines show the DSC curves for peptides CB and CB1 at 20 lM and CB3 at 50 lM in the absence of liposomes Dotted and thin solid lines represent the thermal curves for CB and CB1 ... preceded visible effects in the case of CB and the vesicles were more rapidly disrupted by CB1 than by the parent peptide These observations may suggest that the more hydrophilic and cationic CB1 is ... the specificity of the responses observed Peptide-induced responses were measured by SPR, and results were collected and analysed using the manufacturer’s software and protocols A diagram of the...
- 10
- 443
- 0
Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
Ngày tải lên :
08/03/2014, 09:20
... Tf receptors [39] Then the uptake of Yb-bound Tf by the tumor cells might be much higher than the normal cells and the uptake of iron by the tumor cells will be retarded much more than by the ... suggeststhatthenatureofthefirstkineticphaseofthereaction between Yb3+ and apo-Tf is the same as that of the reaction between Yb3+ and FeC-Tf Therefore the rapid kinetic phase should correspond to the ... shown) The binding mode and the conformational changes induced by Lu3+ and Yb3+: 1H NMR studies These experiments were carried out in order to investigate the order of lobe loading and the conformational...
- 9
- 385
- 0
symbolic space french enlightenment architecture and its legacy by richard a etlin
Ngày tải lên :
21/03/2014, 22:51
... of the building The example of the National Archives and its meaning as a building is the first major example of a modern structure with its legacy rooted to the symbolic space of the past The ... temple to the revolution and the people of France, and furthermore, it was to have a functional purpose within the government The goal of Boullee is to offer the people the feeling that their revolution ... much on the part of the actual structure of the building The description is effective in that it concentrates on the emotion of the building and the metaphorical relationship man has with the contents...
- 4
- 473
- 0
Báo cáo khoa học: "Unsupervised Discovery of Generic Relationships Using Pattern Clusters and its Evaluation by Automatically Generated SAT Analogy Questions" pot
Ngày tải lên :
23/03/2014, 17:20
... include: (1) another label of the cluster (the ‘correct’ answer); (2) three labels of other clusters among the 15; and (3) a pair constructed by randomly selecting words from those making up the various ... that capture this pair by using automated web queries Then we calculated the HITS value for each of the selected pairs and assigned them to clusters with highest HITS value The numbers of pairs ... (schoolbag, book) The three pairs randomly selected from the other 14 clusters were (war, death), (request, license) and (mouse, cat) The pair randomly selected from a cluster not among the 15 clusters...
- 9
- 390
- 0
Báo cáo khoa học: "A Stochastic Language Model using Dependency and Its Improvement by Word Clustering" ppt
Ngày tải lên :
31/03/2014, 04:20
... The terminal symbol is the attribute of a bunsetsu, represented by the product of the head of the content part and that of the function part From the attribute, a word sequence that matches the ... where the functions cont, f u n c and sign take a bun~etsu as their argument and return its content word sequence, its function word sequence and its punctuation respectively In addition, the function ... evaluating the predictive power of the models, we calculated their cross entropy on the test corpus In this process the annotated tree is used as the structure of the sentences in the test corpus Therefore...
- 7
- 352
- 0
Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
Ngày tải lên :
31/03/2014, 07:20
... new bands located between histone H1 and the core histones, which can be attributed to histones d The present study shows a significant increase in the content of p28 and p32 in the nucleus as the ... histone in the fractions was confirmed by SDS/PAGE The protein concentration was determined by the method of Lowry et al [20] using BSA as the standard In the case of histones prepared from DCA-labeled ... CaCl2, and the reaction products were resolved by SDS/ PAGE Three fluorescent bands, the positions of which were identical to those of histones H1, H2B and H3, were detected (Fig 5A) Furthermore,...
- 10
- 503
- 0
Four wave mixing in optical fibers and its applications by osamu aso , masateru tadakuma and shu namiki
Ngày tải lên :
16/07/2014, 08:55
... z is the longitudinal coordinate of the fiber, α is the attenuation coefficient of the fiber, and Ep, Eprobe and Eidler are the electric field of the pumping, probe and idler waves γ is the nonlinear ... coupler There are polarizers at the output terminal of the coupler, and the states of polarization of the pumping and signal at input into the HNL-DSF are in alignment The output was measured by an ... determined by the fiber under measurement and the line width of the pumping light source; and b) the optical power of the probe was set 25 dB lower than pumping power This was to ensure the assumption...
- 6
- 454
- 0
Báo cáo y học: "Novel anti-inflammatory role of SLPI in adipose tissue and its regulation by high fat diet" pptx
Ngày tải lên :
11/08/2014, 03:20
... 2A, B and 2C) Regulation of SLPI expression in adipocytes by inflammatory stimuli and anti-inflammatory effect of SLPI Adipocytes express both TLR2 and TLR4 and the expression of these receptors ... 0.05 Therefore, we examined whether treatment of adipocytes with peptidoglycan and LPS, ligands for TLR2 and TLR4, could alter the expression of SLPI Both peptidoglycan and LPS (Figures 3A and ... 12 weeks At the end of the experiment animals were euthanized by CO2 asphyxiation followed by cervical dislocation All animal care protocols were approved by the Purdue Animal Care and Use Committee...
- 7
- 324
- 0