phyllanthus for its anti cancer properties

... processed for immunofluorescence as previously described (Arciniegas et al., 2005) using the following antibodies: a mouse monoclonal antibody (mab) anti- human integrin β3 (GPIIIa, CD61) and a mab anti- chicken ... polyclonal antibody (pab) phospho-FAK (Tyr397) (Sigma-Aldrich) Negative controls were performed by omitting the primary antibody incubation step or by using non immune serum in place of primary antibody ... caveolae, rich in cholesterol and sphingolipids that serve as a platform for a numerous signaling molecules and compartmentalize them for optimum function Caveolin-1, a major protein constituent of...

Ngày tải lên: 27/06/2014, 17:20

... double-staining the sections with an anti- a-smooth muscle actin antibody (dilution 1:900, clone 1A4, Sigma, Saint Louis, Missouri) and anti- human von Willebrand factor antibody (vWF, dilution 1:900, ... morphometry were performed as described [27] The formalin-fixed and paraffin-embedded lung tissues were subject to sectioning to yield μm thick sections Elastica staining was performed according ... was performed using standard protocols Briefly, paraffinembedded tissue sections were dewaxed, rehydrated and incubated with 0.05% w/v toluidine blue for 2-3 minutes MC density was quantified...

Ngày tải lên: 12/08/2014, 13:22

... oxygen concentration was analyzed continuously before it reached the neonate’s endotracheal tube Nitric oxide was initially administered at 20 ppm for at least h If there was no response while the ... potential of HFOV to reduce the need for extracorporeal membrane oxygenation (ECMO) A number of reports identify a number of infants who, although referred for ECMO, survived without using this ... [12–14] In this study ECMO was not used as an alternative therapy for the INO non-responders because it was not available in our hospital for neonates It has been observed that some infants who showed...

Ngày tải lên: 12/08/2014, 18:20

... strategies for the treatment of acute pancreatitis [7,18,19] To date, it remains unclear whether prophylactic antibiotic treatment or on-demand antibiotic therapy is more beneficial Therefore, we ... significantly by early prophylactic antibiotic treatment but not delayed antibiotic treatment on-demand • Prophylactic antibiotic treatment is superior to treatment on-demand Thus, antibiotics are recommended ... number not for citation purposes) Available online http://ccforum.com/content/12/6/R141 did not show any bacterial infection In these experiments, all animals that received prophylactic antibiotics...

Ngày tải lên: 13/08/2014, 11:23

... used the current 'gold standard' methods to quantify biventricular contractile performance and cardiac loading conditions in an experimental model for acute PHT as well as in healthy animals with ... ± 0.06 7.47 ± 0.04 Values are shown for baseline and in pulmonary hypertension before inhalation and minutes after inhalation of either iloprost or control For the complete experimental time course, ... obviates the need for homeometric autoregulation and allows the right ventricle to return to its baseline (lower) contractile state RV, right ventricular Ventricular afterload was quantified as effective...

Ngày tải lên: 13/08/2014, 11:22

Ngày tải lên: 19/02/2014, 00:20

Ngày tải lên: 09/03/2014, 12:20

... subdivided as follows: For states better than being dead, the value of the health state x/t is bounded by 1.0 for perfect health and zero for states as bad as being dead For states worse than ... is the mean TTO value for each health state and x is a vector of dummy explanatory variables (x∂λ) for each level λ of dimension ∂ of the simplified CAMPHOR classification For example, x31 denotes ... λ = (I don't feel very dependent) For any given health state x∂λ will be defined as follows: x∂λ = 1, if for this state dimension ∂ is at level λ x∂λ = 0, if for this state, dimension ∂ is not...

Ngày tải lên: 18/06/2014, 19:20

... management approach for SScl vasculopathy is outdated and that the widespread use of calcium channel antagonists for RP is probably inappropriate It now appears preferable to use agents for which there ... Functional Class II are awaited The combined vasodilator/antiproliferative/ antifibrotic activity of ERAs raises the exciting prospect for management of peripheral vasculopathy in SScl Last year, the RAPIDS-1 ... them attractive candidates for treating RP Case reports are encouraging but controlled trials are required These approaches depart from the previously dominant strategy for both PAH and RP, which...

Ngày tải lên: 09/08/2014, 06:23

... The PCR program for chymase was initiated by a denaturation step at 94°C, followed by 35 cycles of 94°C for 30 s, 63°C for 30 s and 72°C for min, and a final extension at 72°C for PCR products ... difluoride (PVDF) membranes (Millipore, Bedford, USA) Primary antibodies used were chymase monoclonal antibody (1:1000; Chemicon, Temecula, USA), TGF-b1 polyclonal antibody (1:500; Santa Cruz Biotechnology, ... chymase PCR (738 bp) were (forward) 5’CTG AGA GGA TGC TTC TTC CTG C-3’ and Page of 10 (reverse) 5’-AGA TCT TAT TGA TCC AGG GCC G-3’ [23] Primers for b-actin PCR (194 bp) were (forward) 5’-CCT GTA TGC...

Ngày tải lên: 12/08/2014, 11:21

... 95°C for 30 s, 59°C for 30 s and 72°C for min, and a final extension at 72°C for 10 min; and for b-actin was began with a denaturation step at 95°C, followed by 35 cycles of 95°C for 30 s, 55°C for ... program for p27kip1 was started by a denaturation procedure at 95°C, followed by 35 cycles of 95°C for 30 s, 51°C for 30 s and 72°C for min, and a final extension at 72°C for 10 min; for Skp-2 ... nitrocellulose filter membranes (Millipore, Bedford, USA) The primary antibodies were p27kip1 antibody (1:1000; Millipore, Bedford, USA), Skp-2 polyclonal antibody (1:50; Abcam, Cambridge, UK) and...

Ngày tải lên: 12/08/2014, 13:22

... cells were stained with primary antibodies, anti- mouse/rat CD34 (R&D Systems, Inc Minneapolis, MN) and anti- rat CD45 (BioLegend, San Diego, CA) Following incubation for 30 minutes and washing with ... rat lung: (A) GFP expression Proteins were isolated form rat lungs and Western blot was performed for analysis of GFP protein expression Quantitative data (upper panel), setting hypoxia BMC as ... groups (B) c-kit expression Proteins were isolated form rat pulmonary artery and Western blot was performed for analysis of c-kit expression Quantitative data (upper panel), setting normoxia control...

Ngày tải lên: 12/08/2014, 13:22

... Moreover, it has been reported that hydrogen-rich saline has an antiinflammatory effect [13] Therefore, we hypothesized that the antioxidant and anti- inflammatory effects of hydrogen-rich saline might ... with its anti- oxidative effect Conclusions This study shows that hydrogen-rich saline treatment ameliorates the progression of PH induced by MCT in rats, which may be associated with its anti- inflammatory ... opened, blood was taken from the heart for serum preparation, and lung and heart were processed for histological evaluation or frozen in liquid nitrogen for further analysis Drugs and materials...

Ngày tải lên: 12/08/2014, 13:22

... PBS, v/v), anti- iNOS antibody (1:500 in PBS, v/v), antiP-selectin antibody (BD Pharmingen, CD62P 1:500), anti- ICAM-1 antibody (BD Pharmingen, CD54, 1:500), anti- nitrotyrosine antibody (1:500 in ... incubation for 15 with biotin and avidin (DBA, Milan, Italy), respectively Sections were incubated overnight with anti- TNF-a antibody (1:500 in PBS, v/v), anti- IL-1b antibody (1:500 in PBS, v/v), anti- iNOS ... in PBS, v/v), or PAR antibody (1:500 in PBS, v/v) and anti- TGF-b rabbit polyclonal antibody (1:500 in PBS, v/v) Sections were washed in PBS and incubated with secondary antibody Specific labeling...

Ngày tải lên: 12/08/2014, 13:22

... staining and (b) double immunostaining (anti- a-smooth muscle actin (purple/violet color) and anti- von Willebrand factor (brown color) antibodies) were performed to assess medial wall thickness ... measures of pulmonary vascular remodelling Immunostaining with anti- proliferating cell nuclear antigen (PCNA) antibody (c) was performed to assess the in situ proliferation state of pulmonary ... by echocardiography are shown for all experimental groups Bars represent mean ± SEM One-way ANOVA with Newman-Keuls multiple comparison post-hoc test was performed for statistical analysis ***P...

Ngày tải lên: 12/08/2014, 13:22

... Willebrand factor, anti- vimentin and anti- desmin) for hour at room temperature, and then with secondary antibodies (antimouse IgG conjugated with Alexa-594 fluorescent dye and anti- rabbit IgG conjugated ... acetone for minutes followed by blocking with normal goat serum for 30 minutes as described previously [13] The cells were incubated with primary antibodies (antia-smooth muscle actin (SMA), anti- von ... immunofluorescence staining using anti- von Willebrand factor (Figure 3D), anti- a-SMA (Figure 3D), anti- vimentin (data not shown), and antihuman desmin (data not shown) antibodies The endothelial cell-specific...

Ngày tải lên: 12/08/2014, 14:20

... major biomarker for cardiovascular risk and the main stimulant for hepatic production of C-reactive protein, a compound widely used as a biomarker for atherosclerosis [13] A role for IL-6 in the ... provided Primers used for detecting RNAs for IL-6, sIL-6-R, gp130, ET-1, MCP-1, ICAM, and VCAM in the lungs are listed in table For each sample, the amplification reaction was performed in duplicate ... incubated with rat anti- mouse F4/80 antibody (diluted 1:1000; Abd Serotec) at 4°C overnight with rocking The membrane was then incubated with secondary anti- rat antibody for h at room temperature After...

Ngày tải lên: 12/08/2014, 14:20

... pressure cooking antigen retrieval; nNOS: catalogue reference 610308, BD Biosciences, UK, used at 1:400 dilution along with trypsin antigen retrieval) Bound antibody was detected using goat anti- mouse ... = mild; = moderate Separate quantification was performed for eNOS in small artery endothelium, small artery media, respiratory epithelium, alveolar macrophages Antibody dilutions were chosen ... and then treated for antigen retrieval by microwave pressure cooking or trypsin incubation The sections were then treated to block non-specific binding of primary and secondary antibodies and non-specific...

Ngày tải lên: 12/08/2014, 14:20

... the presence of types I, III and IV anti- collagen antibodies, circulating immune complexes, and the emergence of antinuclear antibodies (ANA) and anti- Scl-70 antibodies [15] Based on Sakkass works ... each, with PBS and incubated with type I or V anti- collagen mouse polyclonal antibody, diluted at 1:50 in PBS, and type III anti- collagen monoclonal antibody (Calbiochem), with a 1:50 dilution ... method (CT) was used for the quantification of gene Table Sequence and description of the genes selected for the study Gene Genbank Accession Number Primer sense (5-3) Primer antisense (5-3) Product...

Ngày tải lên: 12/08/2014, 14:20