phenotyping cardiac gene therapy in mice
gene therapy in cardiovascular disease
Ngày tải lên :
10/04/2014, 22:11
... generating increased collateral flow But documented clinical success in reducing angina scores and improving myocardial perfusion in otherwise untreatable patients has been observed In a porcine ... cardiovascular gene therapy Hum Gene Therapy 10:3031–3044, 1999 Mann MJ, Gibbons GH, Tsao PS Cell cycle inhibition preserves endothelial function in genetically engineered rabbit vein grafts J Clin Invest ... 200 GENE THERAPY IN CARDIOVASCULAR DISEASE Mann MJ, Whittemore AD, Donaldson MC Preliminary clinical experience with genetic engineering of human vein grafts: Evidence for target gene inhibition...
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gene therapy in the treatment of cancer
Ngày tải lên :
10/04/2014, 22:11
... molecules in the interleukin family, which have similar effects and have also been studied, including interleukin (IL-4), interleukin (IL-7), and interleukin 12 (IL-12) IL-12 is a heterodimer consisting ... translational initiation site or a splicing site on the gene (Fig 10.4) This binding represents an antigene approach that would inhibit genetic information flow (DNA–RNA–protein) The antigene approach ... Lipofection 250 GENE THERAPY IN THE TREATMENT OF CANCER now in development Such inhibition results not only in growth inhibition in vitro but also results in growth inhibition of tumors in animal models...
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Chapter 065. Gene Therapy in Clinical Medicine (Part 1) doc
Ngày tải lên :
07/07/2014, 01:20
... Main Viral Vectors Features Retroviral Lentiviral Adenoviral AAV Hum Foamy Vir advantages gene transfer in gene transfer in effective dividing cells in few gene expre transduced transducing inflammatory ... steps in which the donated DNA enters the target cell and begins expression is referred to as transduction Gene delivery can take place in vivo, in which the vector is directly injected into the ... inflammatory in both div tissues various tissues responses, and nondiv nonpathogenic cells Main disadvantages Theoretical Might risk of insertional induce mutagenesis (occurred in oncogenesis some...
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Chapter 065. Gene Therapy in Clinical Medicine (Part 2) pdf
Ngày tải lên :
07/07/2014, 01:20
... from J Gene Med New Jersey, Wiley, 2006.) Gene Transfer for Genetic Disease Gene transfer strategies for genetic disease generally involve gene addition therapy This approach most commonly involves ... using integrating vectors in hematopoietic cells must include a plan for monitoring sites of insertion and clonal proliferation Strategies to overcome this complication have included employing ... "suicide" gene cassette in the vector, so that errant clones can be quickly ablated; or using "insulator" elements in the cassette, which can limit the activation of genes surrounding the insertion...
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Chapter 065. Gene Therapy in Clinical Medicine (Part 3) docx
Ngày tải lên :
07/07/2014, 01:20
... by mutations in several different genes; ~15% of cases of LCA are due to a mutation in a gene, RPE65, encoding a retinal pigment epithelial protein In dogs with a null mutation in RPE65, sight ... knockdown of VEGF Gene Therapy for Cancer The majority of clinical gene transfer experience has been in subjects with cancer (Fig 65-1) As a general rule, a feature that distinguishes gene therapies ... and will likely be tested in upcoming trials Administration of an AAV vector expressing factor IX to the liver in humans with hemophilia resulted in therapeutic circulating levels at the highest...
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Chapter 065. Gene Therapy in Clinical Medicine (Part 4) ppt
Ngày tải lên :
07/07/2014, 01:20
... with immune-enhancing genes encoding cytokines, chemokines, or co-stimulatory molecules Sustained clinical responses provide evidence that the transduced cells can act as a vaccine In a related approach, ... transduced with genes encoding a T cell receptor–like molecule, with a tumor antigen–binding domain fused to an intracellular signaling domain to allow T cell activation, thereby converting normal ... particular value in treating minimal residual disease after other anticancer modalities Gene transfer strategies have also been developed for inhibiting tumor angiogenesis These have included constitutive...
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Chapter 065. Gene Therapy in Clinical Medicine (Part 5) ppt
Ngày tải lên :
07/07/2014, 01:20
... recombinant proteins are recent examples Gene therapeutics, which entered clinical testing in the early 1990s, are well along in the course of development, and are likely to become increasingly ... Taking History from Subjects Enrolled in Gene Transfer Studies Elements of History for Subjects Enrolled in Gene Transfer Trials What vector was administered? Is it predominantly integrating ... manuscript Further Readings Hacein-Bey-Abina S et al: LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-XI Science 302:415, 2003 [PMID: 14564000] Lin E, Nemunaitis...
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Báo cáo y học: " Gene therapy in animal models of rheumatoid arthritis: are we ready for the patients" pdf
Ngày tải lên :
09/08/2014, 01:24
... transgene expression in the joint was high [48], but interestingly the transgene expression also increased with joint inflammation [49] Clinical efficacy of local gene therapy with recombinant ... activity in murine collagen-induced arthritis Gene Ther 2003, 10:19501960 Yin G, Liu W, An P, Li P, Ding I, Planelles V, Schwarz EM, Min W: Endostatin gene transfer inhibits joint angiogenesis ... overexpressed (using recombinant proteins or gene transfer, or in transgenic mice) in animals, including mice, rats and rabbits [9] There is consensus that TNF is a potent proinflammatory mediator,...
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Báo cáo y học: "The 3rd International Meeting on Gene Therapy in Rheumatology and Orthopaedics" pot
Ngày tải lên :
09/08/2014, 07:20
... in the meeting, Haim Burstein (Targeted Genetics Inc., Seattle, WA, USA) described the first human clinical trial using AAV in arthritis gene therapy John Mountz (University of Alabama, Birmingham, ... experiments in horses in which allogeneic chondrocytes were transduced with equine insulin-like growth factor-1 or human bone morphogenetic protein (BMP)-7, incorporated into a fibrin clot, and ... methods for delivering proteins efficiently into cells by attaching protein transduction domains to their amino-termini One such protein transduction domain is derived from the TAT protein of HIV; Jeffrey...
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Báo cáo khoa học: "Changes in pulse pressure variability during cardiac resynchronization therapy in mechanically ventilated patients" pps
Ngày tải lên :
13/08/2014, 03:20
... studies using invasive blood pressure measurement Conclusion Our findings indicate that changes in cardiac performance have a significant influence on respiratory fluctuations in PP This interaction ... recording Investigators who determined cardiac output invasively or by echocardiography found a median increase of 8% and mean increases of 10% and 15%, respectively, during biventricular pacing ... decrease in PP variation after fluid loading) [1] In our patients with severely impaired cardiac function, PP variation remained (even during biventricular pacing) below the threshold value indicating...
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Báo cáo y học: "Genomic analysis of metabolic pathway gene expression in mice" pps
Ngày tải lên :
14/08/2014, 14:21
... expressed genes and how many of the eQTLs specific for the 77 pathway genes defined above mapped to each interval In general, assuming under the null hypothesis that the genomic location of genes influencing ... with genetically altered mice, we have studied a heterogeneous population where the genetic variations leading to the phenotypes and the changes in gene expression are all naturally occurring In ... originating from an F2 intercross carried out between mouse strains C57BL/6J and DBA/2J This intercross encompassed a two-phase study as described in Colinayo et al [28], using 142 female F2 mice...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
Ngày tải lên :
14/08/2014, 19:22
... (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each other (Figure 5) Within the ... purposes) Genetic Vaccines and Therapy 2004, 2:7 http://www.gvt-journal.com/content/2/1/7 Figure Bioluminescence imaging of nude mice carrying HSV-TK-Luc expressing U87MG gliomas Bioluminescence imaging ... protein known to be overexpressed in cancer cells [27], and (4) coelenterazine is much more expensive than D-Luciferin Iyer et al [28] examined noninvasive imaging using PET and BLI in CD-1 mice...
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Báo cáo sinh học: "Recombinant λ-phage nanobioparticles for tumor therapy in mice models" pdf
Ngày tải lên :
14/08/2014, 19:22
... The E7 gene with flanking EcoRI and Xho I sites, respectively, was synthesized by ShineGene (Shanghai ShineGene Molecular Biotech, Inc) and cloned into pUC18 vector E coli bacterial strain DH5α ... conjugated to alkaline phosphatase (Sigma, St Louis, MO) in secondary antibody solution Color was developed by incubating the membrane in alkaline phosphate buffer containing NBT (nitro blue ... sequences containing MHC class I and/or class II epitopes linked to nano-beads induce strong immunity and inhibition of growth of antigen-specific tumour challenge in mice Vaccine 2004, 23:258-266...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
Ngày tải lên :
16/03/2014, 18:20
... neuronal intranuclear inclusions (NIIs) containing the human transgene-encoded amino terminus of human huntingtin form more slowly throughout the brain tissue in R6/1 compared to R6/2 mice [29,30] ... protein; RAR, retinoic acid nuclear receptor; WHN, winged helix protein; BKLF, basic krueppel-like factor; ZF5, zinc finger domain; MYT1, zinc finger TF involved in primary neurogenesis; E2A proteins, ... affected in both strains of R6 transgenic HD mice It appeared that the amino terminus of mutant huntingtin containing an expanded polyglutamine tract caused the steady-state CB1 mRNA levels in the...
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Báo cáo khoa học: Disruption of the gene encoding 3b-hydroxysterol D14-reductase (Tm7sf2) in mice does not impair cholesterol biosynthesis pdf
Ngày tải lên :
23/03/2014, 06:20
... polypeptide 39 predicted gene, EG432995 prominin NIPA-like domain containing glutathione S-transferase, alpha (Yc2) flavin containing monooxygenase very low density lipoprotein receptor expressed ... cerevisiae) Shc SH2-domain binding protein bromodomain and WD repeat domain containing serum amyloid A cell division cycle homolog A (S pombe) heat shock 105kDa ⁄ 110kDa protein zwilch, kinetochore associated, ... containing exons 1–5 of the gene (Fig 2B) The neomycin phosphotransferase gene (neo) was inserted in a SalI restriction site generated by in vitro mutagenesis in exon The vector was linearized with NotI...
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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
Ngày tải lên :
24/03/2014, 04:21
... shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of lentiviral vector carrying the human IDUA cDNA Gene transfer vector includes ... subcloned into plasmid pRRLsinPPT.CMV.WPRE after double digestion of this vector with MscI and MluI to obtain the self inactivating (sin) gene transfer construct PRRLsinPPT.CMV IDUA.WPRE The packaging ... cross-correction of co-incubated, untransduced cells Lentiviral vectors have been shown to effectively transduce genes into a range of both dividing and nondividing cell types, including neurons, retinal cells,...
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ethical issues in molecular medicine and gene therapy
Ngày tải lên :
10/04/2014, 22:10
... distinctions in mind we can raise some additional ethics issues regarding gene therapy from a social point of view Is there anything intrinsically morally objectionable about gene therapy in any ... would support a presumption in favor of continuing the development of germline genetic engineering That is, we have argued that germline genetic engineering is not intrinsically morally objectionable ... engaged in germline genetic engineering Failing that, however, we would still have the alternative of creating multiple eight-cell embryos in vitro, genetically analyzing them, then implanting only...
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vectors in gene therapy
Ngày tải lên :
10/04/2014, 22:11
... transfer genetic information into nondividing cells in vivo In addition, expression has been maintain for up to one year Further experiments to determine if AAV vectors integrate or are maintained in ... Replication-incompetent retroviral vectors can be easily generated by deleting retroviral genes and adding gene( s) of interest Vectors can be produced in packaging cell lines that express packaging genes ... replication of an EBV origin of replication Plasmids containing these replication origins and relevent appropriate proteins activating origins are maintained longer in cells in vitro and in vivo than plasmids...
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Báo cáo sinh học: " The state of the art of adeno-associated virus-based vectors in gene therapy" ppt
Ngày tải lên :
18/06/2014, 18:20
... of expressing foreign genes in mammalian cells [8,9], in the early 1980s, AAV2 vectors have rapidly gained popularity in gene therapy applications, due to some of their distinguishing biological ... and overcome, rAAV-based gene transfer vectors still represent one of the most promising gene therapy systems and gain increasing popularity In a search for "AAV gene therapy" , 1,016 PubMed references ... most promising and distinguishing features of AAV vectors However, there are also a few drawbacks in using AAV vectors for gene therapy applications (1) Their size limits the insertion of gene expression...
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